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The Sensitivity Pattern of Extended Spectrum Beta Lactamase-Producing Bacteria Against Six Antibiotics that Routinely Used in Clinical Setting Kuntaman, Kuntaman; Santoso, Sanarto; Wahjono, Hendro; Mertaniasih, Ni Made; Lestari, Endang S.; Farida, Helmia; Hapsari, Rebriarina; Firmanti, Stefani Candra; AS, Noorhamdani; Santosaningsih, Dewi; Purwono, Priyo Budi; Kusumaningrum, Deby
Journal of the Indonesian Medical Association Vol. 61 No. 12 December 2011
Publisher : Journal of the Indonesian Medical Association

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The validated study of extended-spectrum b-lactamase (ESBL) producing bacteria inIndonesia is scarce. Multi-centre study on susceptibility of ESBLs producers is our point of view. A survey was carried out in three teaching hospitals in Surabaya (Dr. Soetomo), Malang (Dr. Saiful Anwar) and Semarang (Dr. Kariadi). Clinical ESBL-producers were collected in over four months period (January to April 2010) up to 300 strains. The susceptibility against 6antibiotics below were used as a point of view in analysis. As many as 300 isolates were collected, 140 (Surabaya), 85 (Semarang) and 75 (Malang) respectively. The three most prevalent ESBL producers were: E. coli (42.7%), Klebsiella pneumoniae (47.3%) and Enterobacter spp (7%). The other 9 strains were: Citrobacter spp, Klebsiella oxytoca, Proteus mirabilis and Serratia spp. The susceptibility analysis was then performed on the three most prevalent isolates. The sensitivity rate of E. coli, Klebsiella pneumoniae and Enterobacter spp against tested antibiotics were 3%, 4% and 5% for cefotaxim; 91%, 87% and 90% for Amikacin; 27%, 54% and 43% for Ciprofloxacin; 98%, 93% and 100% for Cefoperason-Sulbactam; 100%, 96% and 100% for Meropenem; 95%, 94% and 86% for Fosfomycin. As a conclusion, we found that amikacin, cefoperason-sulbactam, meropenem and fosfomycin, are prospective for emperic therapy in clinical setting of health services where ESBL producing bacteria were prevalent as causative. J Indon Med Assoc. 2011;61:482-6.Keywords: Extended Spectrum Beta Lactamases (ESBL), antibiotic, antimicrobial resistance,sensitivity.
PRODUKSI IgA & IgG MUKOSAL DAN SISTEMIK SETELAH IMUNISASI PER ORAL DENGAN PROTEIN AdhO36 SALMONELLA TYPHI PADA MENCIT BALB/C Santoso, Sanarto
Jurnal Kedokteran Brawijaya Vol 19, No 2 (2003)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

ABSTRACT An experiment has been conducted to determine the production of mucosal and systemic IgA and IgG after oral immunization with AdhO36 protein Salmonella typhi using CTB as adjuvant. The results of ELISA Test, shows that AdhO36 protein Salmonella typhi were highly significantly (p = 0.001) able to induce the production of S-IgA (secretory IgA), as compared to control and CTB treatment. The analysis of variance results show that the effect of AdhO36 protein to the IgA content in serum is high significantly different to IgA content in mucus (p=0.001). This finding is relevant to the previous experiments that the oral administration of AdhO36 protein can inhibit the in vivo adhessive process of Salmonella typhi at enterocytes of Balb/c mice. Therefore, it can be concluded that AdhO36 protein  Salmonella  typhi  is  a  potential  mucosal  immunogen  which  can  induce  mucosal  immune  response  as  the  results  of  the formation of protective S-IgA, which in turn, can inhibit the adhesive process as the initial stage of infection process at enterocytes of Balb/c mice.  Apart from S-IgA,
DIARE ROTAVIRUS PADA ANAK DIBAWAH USIA 3 TAHUN YANG DIRAWAT DI RSU DR. SAIFUL ANWAR MALANG TAHUN 2005 (PRELIMINARY STUDY) Santoso, Nurtjahjo Budi; Hamid, Aulia Abdul; Santoso, Sanarto
Jurnal Kedokteran Brawijaya Vol 20, No 2 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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ABSTRACT Rotavirus is the most common cause of acute diarrhea among children worldwide. The majority of the cases required hospitalization. Epidemiologic pattern and clinical manifestation could be helpful in establishing the proper diagnosis and management. To find out prevalence and the clinical pictures of rotavirus diarrhea among children under three years of ages with acute diarrhea admitted in the Department of Child Health Dr. Saiful Anwar General Hospital. The stools of children under three years of age with acute diarrhea between March 1st and May 1st, 2005 were assessed for rotavirus using Latex agglutination test (Slidex, Rota K2, bio Merieux). Age, sex, clinical sign and symptoms and breast-fed were recorded. The severity of acute diarrhea was estimated using WHO criteria. Statistical analysis were performed using EpiInfo 2000 program with x2 or x2 for trend. From the 40 patients with acute diarrhea were found 16 patients (40%) with rotavirus positive, both male and female were 8 patients (50%). Distribution of rotavirus positive patients in age group were 0-5 months 3/16 (18,7%), 6-11 months 6/16 (37,5%), 12-23 months 6/16 (37,5%) and 24-36 months 1/16 (6,3%). Clinical manifestations showed that most children had fever (75%), vomiting (87.5%) and dehydration (87.5%). Rotavirus infection among breast-fed patients were found less than bottle-fed patients.
PERBEDAAN POLA RESISTENSI Staphylococcus koagulase negatifISOLAT DARAH TERHADAP ANTIBIOTIKA DI RSU Dr SAIFUL ANWAR MALANG TAHUN 2000-2001 DENGAN 2004-2005 Dzen, Sjoekoer M.; Santoso, Sanarto; Roekistiningsih, Roekistiningsih; Santosaningsih, Dewi
Jurnal Kedokteran Brawijaya Vol 21, No 3 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Coagulase negative staphylococci (CoNS) are the common bacterias causing hospital-acquired infection manifested as bacteremia. The antibiotics therapy against bacterial infection is mostly empiric, whereas the bacterial pattern and its sensitivity to antibiotics differ between region and from year to year. The objective of this study wasto determine the changes of prevalence and antibiotics sensitivity pattern of CoNS, the etiologic agent of bacteremia in patients hospitalised in Dr.Saiful Anwar General Hospital, Malang. Culture and antibiotic sensitivity test wereperformed on 650 and 1063 blood samples during the year of 2000-2001and 2004-2005 respectively in the Laboratory of Microbiology, Faculty of Medicine, Brawijaya University / Dr.Saiful Anwar General Hospital in Malang following NCCLS  1997. ChiSquare test was used to analysed of data. The result of this study revealed that the CoNS was the most isolate found in the blood samples and its resistance to beta lactam antibiotics tends to increase statistically. Key words:Coagulase negative staphylococci, antibiotics sensitivity pattern
Evaluation of latex agglutination test and oxacillin resistant screening agar base (ORSAB) medium for the detection of oxacillin resistant coagulase negative Staphylococci (ORCoNS) (Preliminary study) Dzen, Sjoekoer M.; Santoso, Sanarto; Roekistiningsih, Roekistiningsih; Santosaningsih, Dewi
Medical Journal of Indonesia Vol 16, No 4 (2007): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.474 KB) | DOI: 10.13181/mji.v16i4.278

Abstract

Coagulase negative Staphylococci (CoNS) are recognized as an important cause of nosocomial infection, especially in neonates and patients with indwelling prosthetic devices. The CoNS resistance rate to oxacillin has been increasing. Therefore, rapid and accurate detection of oxacillin resistance is essential in order to determine the most appropriate antimicrobial therapy. This study aimed to prove that latex agglutination test and oxacillin resistant screening agar base (ORSAB) medium can be used for rapid detection of oxacilllin resistant CoNS (ORCoNS). Latex agglutination test and ORSAB medium compared with the conventional method was conducted in this study toward 30 clinical isolates of CoNS for the detection of ORCoNS. Mc Nemar test was used to analyze the data. The study result revealed that there was no significant difference (P>0.05) in terms of ORCoNS detection between the latex agglutination test and ORSAB medium on the one hand, and the conventional method on the other. It is concluded that latex agglutination test and ORSAB medium can be used for rapid detection of ORCoNS. (Med J Indones 2007; 16:228-32)Keywords: nosocomial infection, rapid detection, mecA gene
Identifikasi Protein Adhesi Pili Proteus Mirabilis P355 dan Protein Reseptor pada Vesika Urinaria Kelinci Mufida, Dian Chusna; Sumarno, Sumarno; Santoso, Sanarto
The Journal of Experimental Life Science Vol 1, No 1 (2011)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (852.907 KB) | DOI: 10.21776/ub.jels.2011.001.01.01

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Infeksi saluran kemih (ISK) merupakan infeksi nosokomial yang sering terjadi di rumah sakit. Penyebab ISK nosokomial ini diantaranya adalah Proteus mirabilis p355 dan sering dijumpai pada pasien yang memakai kateter. Proteus mirabilis mempunyai berbagai macam faktor virulensi, diantaranya adalah fimbria atau pili. Tujuan penelitian ini untuk mengidentifikasi protein pili Proteus mirabilis (molekul adhesin) serta mencari protein reseptor bakteri tersebut pada epitel vesika urinaria kelinci. Penelitian ini dilakukan dengan isolasi protein pili, selanjutnya dilakukan uji hemaglutinasi dan uji hambat adhesi. Uji hambat adhesi mempergunakan protein pili  yang telah dielektroelusi dan didialisa, disalutkan pada sel epitel vesika urinaria kelinci dengan dosis 400 µl, 200 µl, 100 µl, 50 µl, 25 µl, 12,5 µl dan 0 µl sebagai kontrol. Selanjutnya dilakukan identifikasi protein reseptor dengan cara menyalut bakteri Proteus mirabilis dengan matriks ekstra seluler dari epitel vesika urinaria kelinci. Hasil penelitian ini menunjukkan adanya reaksi hemaglutinasi dari protein pili dengan berat 35,2 kDa dengan titer tertinggi 1/521. Hasil uji hambat adhesi epitel vesika urinaria yang disalut protein pili dengan berat 35,2 kDa menunjukkan bahwa semakin tinggi dosis protein yang diberikan, semakin sedikit bakteri yang melekat pada epitel vesika urinaria. Penurunan ini terjadi secara signifikan dengan indeks regresi   (r) = 0,95 dan p-value = 0,00. Berdasarkan penelitian ini dapat disimpulkan bahwa protein pili Proteus mirabilis dengan berat molekul 35,2 kDa merupakan protein adhesin, sedangkan protein reseptor Proteus mirabilis diprediksi mempunyai berat molekul, 36 kDa, 24,5 kDa dan 24 kDa.Kata kunci : adhesi, pili, protein reseptor, Proteus mirabilis
PENGARUH METODE PENGOLAHAN (JUICING DAN BLENDING) TERHADAP KANDUNGAN QUERCETIN BERBAGAI VARIETAS APEL LOKAL DAN IMPOR (Malus domestica) Cempaka, Anggun Rindang; Santoso, Sanarto; Tanuwijaya, Laksmi Karunia
Indonesian Journal of Human Nutrition Vol 1, No 1 (2014)
Publisher : Jurusan Gizi, Fakultas Kedokteran, Universitas Brawijaya Malang

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Abstract

Abstrak Quercetin merupakan salah satu flavonoid yang dipercaya dapat melindungi tubuh dari beberapa penyakit degeneratif dengan mencegah proses peroksidasi lemak. Apel merupakan buah yang kaya kandungan quercetin, banyak dikonsumsi oleh masyarakat, serta mudah didapatkan. Kandungan quercetin berbeda pada setiap buahnya, bergantung pada varietas, proses pengolahan, kondisi pertumbuhan, nutrisi tanaman, dan lama penyimpanan. Penelitian ini bertujuan untuk mengetahui pengaruh proses pengolahan (juicing dan blending) terhadap kandungan quercetin pada berbagai varietas apel lokal dan impor (Malus domestica). Kandungan quercetin diukur dari 4 varietas apel yang berbeda yaitu untuk apel lokal diwakili oleh varietas Rome beauty dan manalagi, sedangkan untuk apel impor diwakili oleh varietas fuji dan Red delicious yang masing-masing dibagi dalam 3 kelompok, yaitu kelompok 1 apel segar sebagai kontrol, kelompok 2 jus apel (juicing), dan kelompok 3 smoothie apel (blending). Penelitian ini menggunakan desain eksperimental laboratorik. Unit eksperimen penelitian dipilih dengan menggunakan metode RAK dengan 3 kali replikasi. Pengukuran kandungan quercetin dianalisis dengan menggunakan metode ekstraksi sampel dalam larutan etanol dan spektrofotometer. Berdasarkan hasil penelitian, apel segar mengandung quercetin paling banyak, diikuti oleh jus apel (juicing), dan smoothie apel (blending). Sedangkan varietas apel yang paling banyak mengandung quercetin adalah apel Rome beauty, diikuti oleh manalagi, fuji, dan Red delicious. Berdasarkan hasil tersebut, dapat disimpulkan bahwa varietas apel dan proses pengolahan yang berbeda dapat mempengaruhi kandungan quercetin apel segar dan jus apel secara signifikan (p < 0.05). Kata kunci :    quercetin, metode pengolahan, juicing, blending, varietas apel Abstract Quercetin is kind of flavonoid which can protect the body from several degenerative diseases by preventing lipid peroxidation process. Apple is one of fruits which contains abundant source of quercetin, being consumed generally and affordable by most of people in the world. Quercetin content is different on each apples, depending on varieties, processing methods, growing conditions, plants nutritions, and storages. This study aimed to determine the effect of processing methods (juicing and blending) on quercetin content of local and import varieties of apple (Malus domestica). Quercetin content was measured from 4 different varieties of apples, some of which are local apples which are represented by Rome beauty and manalagi, and import apples which are represented by fuji and Red delicious. All of apple varieties is divided into 3 groups in which group 1 is apple fresh, group 2 is apple juice, and group 3 is apple smoothie. This study used laboratory experimental design. Unit experiment was selected using Randomized Group Design with 3 replication of each. Quercetin content measurement was analyzed by using extraction method in etanol solution and spectrophotometer. Based on the results, fresh apples contain quercetin at most of all, followed by apple juices (juicing), and apple smoothie (blending). Whereas apple variety which have highest content of quercetin is rome beauty, followed by manalagi, fuji, and red delicious. Based on these, it can be concluded that different processing methods and varieties of apples affect quercetin content of fresh apples and both of apple juices (juicing and blending) significantly (p<0.05). Keywords : quercetin, processing methods, juicing, blending, apple varieties
PERBEDAAN POLA RESISTENSI Staphylococcus koagulase negatifISOLAT DARAH TERHADAP ANTIBIOTIKA DI RSU Dr SAIFUL ANWAR MALANG TAHUN 2000-2001 DENGAN 2004-2005 Dzen, Sjoekoer M.; Santoso, Sanarto; Roekistiningsih, Roekistiningsih; Santosaningsih, Dewi
Jurnal Kedokteran Brawijaya Vol 21, No 3 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.523 KB) | DOI: 10.21776/ub.jkb.2005.021.03.4

Abstract

Coagulase negative staphylococci (CoNS) are the common bacterias causing hospital-acquired infection manifested as bacteremia. The antibiotics therapy against bacterial infection is mostly empiric, whereas the bacterial pattern and its sensitivity to antibiotics differ between region and from year to year. The objective of this study wasto determine the changes of prevalence and antibiotics sensitivity pattern of CoNS, the etiologic agent of bacteremia in patients hospitalised in Dr.Saiful Anwar General Hospital, Malang. Culture and antibiotic sensitivity test wereperformed on 650 and 1063 blood samples during the year of 2000-2001and 2004-2005 respectively in the Laboratory of Microbiology, Faculty of Medicine, Brawijaya University / Dr.Saiful Anwar General Hospital in Malang following NCCLS  1997. ChiSquare test was used to analysed of data. The result of this study revealed that the CoNS was the most isolate found in the blood samples and its resistance to beta lactam antibiotics tends to increase statistically. Key words:Coagulase negative staphylococci, antibiotics sensitivity pattern
EKSPRESI PROTEIN ADHF36 PADA PERUBAHAN OSMOLARITAS SERTA pH LINGKUNGAN HIDUP SALMONELLA TYPHI SECARA IN VITRO Kundera, I Nengah; Santoso, Sanarto; a, Aulanni’am; Winarsih, Sri
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.445 KB) | DOI: 10.21157/j.ked.hewan.v6i1.351

Abstract

Penelitian ini bertujuan mengamati stabilitas ekspresi protein AdhF36 Salmonella typhi pada perlakuan osmolaritas dan pH dengan metode eksperimen laboratorium-eksploratif. Hasil penelitian ini menunjukkan perbedaan ekspresi protein AdhF36 Salmonella typhi pada perlakuan osmolaritas dengan pH. Hasil SDS-PAGE menunjukkan bahwa pita protein AdhF36 tetap terekspresi pada perlakuan osmolaritas 50-350 mM, sebaliknya pita protein ini tidak terdeteksi pada perlakuan pH 4,5-6,0. Hal ini didukung oleh hasil uji Western Blot yang berhasil mendeteksi protein AdhF36 hanya pada perlakuan osmolaritas. Hasil ini membuktikan bahwa Salmonella typhi lebih toleran terhadap perubahan osmolaritas dibandingkan dengan pH.
Tingginya Prevalensi MRSA pada Isolat Klinik Periode 2010- 2014 di RSUD Dr. Saiful Anwar Malang, Indonesia Erikawati, Dewi; Santosaningsih, Dewi; Santoso, Sanarto
Jurnal Kedokteran Brawijaya Vol 29, No. 2 (2016)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.762 KB) | DOI: 10.21776/ub.jkb.2016.029.02.9

Abstract

Methicillin Resistant Staphylococcus aureus (MRSA) merupakan bakteri patogen terpenting penyebab infeksi terkait perawatan di rumah sakit di dunia. Penelitian ini bertujuan untuk membandingkan prevalensi dan pola resistensi antibiotik dari isolat MRSA di RSUD Dr. Saiful Anwar Malang, Jawa Timur, Indonesia selama kurun waktu 2010-2014. Isolat MRSA didapatkan dari berbagai spesimen klinik, seperti darah, pus, sputum, dan urin. Kami mendeteksi isolat MRSA secara fenotipik, selanjutnya melakukan uji kepekaan antibiotik sesuai standar dari Clinical and Laboratory Standards Institute (CLSI) 2014. Secara keseluruhan, didapatkan 772 isolat Staphylococcus aureus, 38,2% diantaranya merupakan isolat MRSA. Prevalensi MRSA tertinggi didapatkan pada tahun 2012 (45,3%), sedangkan prevalensi terendah pada tahun 2013 (33,5%). Kasus MRSA paling sering ditemukan dari pus (49%). Ditemukan penurunan resistensi isolat MRSA dari darah terhadap chloramphenicol (p<0,05), dari pus terhadap tetracycline, dari sputum terhadap erythromycin dan trimethoprim-sulfamethoxazole. Sementara itu didapatkan peningkatan resistensi terhadap chloramphenicol (p<0,05). Dapat disimpulkan bahwa prevalensi MRSA di RSUD dr. Saiful Anwar Malang, Indonesia cukup tinggi dan terjadi perubahan pola sensitifitas terhadap beberapa antibiotik.Kata Kunci: MRSA, resistensi antibiotik, rumah sakit