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Journal : Jurnal AgroBiogen

Phylogenetic and Maturity Analyses of Sixty Soybean Genotypes Used for DNA Marker Development of Early Maturity Quantitative Trait Loci in Soybean

Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
Publisher : Jurnal AgroBiogen

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Abstract

The Indonesian soybean productivity is still very low with the national average of 1.3 t/ha. One means to improve national soybean productivity is by manipulating harvest index by cultivating very early maturing soybean cultivars. Development of early maturing soybean cultivars can be expedited by using marker-aided selection. The objective of this study was to select parental lines having contrasted maturity traits and selected parents must be genetically distance. The parents then were used to develop F2 populations for detecting early maturity QTL in soybean. Maturity tests of 60 soybean genotypes were conducted at two locations, Cikeumeuh (Bogor) and Pacet (Cianjur) using a randomized block design with three replications. Genomic DNA of the 60 genotypes were analyzed using 18 SSR markers and genetic relationship was constructed using the Unweighted Pair-Group Method Arithmatic through Numerical Taxonomy and Multivariate System program version 2.1-pc. Results showed that the 60 genotypes demonstrated normal distribution in both locations for days to R1 (32-48d), days to R3 (35-55d), days to R7 (75-92d), and days to R8 (78-99d). Four early maturing genotypes and three late genotypes were obtained. Total SSR alleles observed were 237 with average allele per locus of 12.6 (3-29), and average PIC value of 0.78 (0.55-0.89). Genetic similarity among genotypes ranges from 74.8-95%. At similarity level 77% divided the genotypes into six clusters (the four selected early maturing genotypes located in clusters III and IV, while the three late genotypes located in cluster II). Based on maturity data, pubescent color, and phygenetic analysis seven parents were selected (four early maturing genotypes B1430, B2973, B3611, B4433 and three late genotypes B1635, B1658, and B3570). Twelve F2 populations were developed with the aid of SSR markers Satt300 dan Satt516. Two of the populations will be used to develop DNA markers for earliness in soybean.

Phylogenetic and Maturity Analyses of Sixty Soybean Genotypes Used for DNA Marker Development of Early Maturity Quantitative Trait Loci in Soybean

Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Original Source | Check in Google Scholar | Full PDF (183.856 KB)

Abstract

Phylogenetic and Maturity Analyses of Sixty SoybeanGenotypes Used for DNA Marker Development of EarlyMaturity Quantitative Trait Loci in Soybean. I MadeTasma, Dani Satyawan, Ahmad Warsun, MuhamadYunus, and Budi Santosa. The Indonesian soybeanproductivity is still very low with the national average of 1.3t/ha. One means to improve national soybean productivity isby manipulating harvest index by cultivating very earlymaturing soybean cultivars. Development of early maturingsoybean cultivars can be expedited by using marker-aidedselection. The objective of this study was to select parentallines having contrasted maturity traits and selected parentsmust be genetically distance. The parents then were used todevelop F2 populations for detecting early maturity QTL insoybean. Maturity tests of 60 soybean genotypes wereconducted at two locations, Cikeumeuh (Bogor) and Pacet(Cianjur) using a randomized block design with threereplications. Genomic DNA of the 60 genotypes wereanalyzed using 18 SSR markers and genetic relationship wasconstructed using the Unweighted Pair-Group MethodArithmatic through Numerical Taxonomy and MultivariateSystem program version 2.1-pc. Results showed that the 60genotypes demonstrated normal distribution in bothlocations for days to R1 (32-48d), days to R3 (35-55d), days toR7 (75-92d), and days to R8 (78-99d). Four early maturinggenotypes and three late genotypes were obtained. TotalSSR alleles observed were 237 with average allele per locusof 12.6 (3-29), and average PIC value of 0.78 (0.55-0.89).Genetic similarity among genotypes ranges from 74.8-95%.At similarity level 77% divided the genotypes into six clusters(the four selected early maturing genotypes located inclusters III and IV, while the three late genotypes located incluster II). Based on maturity data, pubescent color, andphygenetic analysis seven parents were selected (four earlymaturing genotypes B1430, B2973, B3611, B4433 and threelate genotypes B1635, B1658, and B3570). Twelve F2populations were developed with the aid of SSR markersSatt300 dan Satt516. Two of the populations will be used todevelop DNA markers for earliness in soybean.

Co-Authors Abdurrahman, Ahmad Fauzan Abiyoga, Gerald Arsa Adhiyaksa Ade Lia D. Safitri Adrianus Yosep Ago, Adrianus Yosep Agus Sasmito Aribowo Ahmad Warsun Ali Ridho Barakbah Andri S, Andri Andri Sukeksi Anin, Vitry Agrida Anissa Wulandari, Anissa Antariksa Antariksa Ari Yanuar Ridwan, Ari Yanuar Ari Yanuar, Ari Bambang Utoyo Boyke Frahmana, Boyke Budi Sulistyo Budianto, Anwar Budiyono Budiyono Buru, Romika Jibrael Dani Satyawan Deniswara, Cindy Dessyanto Boedi P Destaria Madya Verawaty, Destaria Madya Dida Diah Damayanti Didi Setya, Didi Didik Sumanto Dinda Sekar Putri, Dinda Sekar Djoko Legono Dwi Sulistyoningsih Dwiska Aini Nurrahma, Dwiska Aini Eka Fitasari Eka Puji Hastuti, Eka Puji Ekawati, Fitri Eko Prasetyo Nugroho Endang Purwaningsih Erik Kurniawan, Erik Ethica, Stalls Norma Fajar Kurnia Fani Yayuk Supomo Fauzia Hulopi Gita Purnama Sari, Gita Purnama Hairurraziqin Hairurraziqin, Hairurraziqin Hananta, Yan’s Dianaga Hani Purnamasari, Hani Hariyadi Hariyadi Hariyana, Bambang Helmi Helmi Henna Ria Sunoko, Henna Ria Henna Rya Sunoko Henna Rya Sunoko Herry Sofyan Hertanto Wahyu Subagio Hertanto Wahyu Subagyo Heru Cahya Rustamaji I Ketut Eddy P, I Ketut I Ketut Eddy P. I Made Tasma Ial Irwan Arahman, Ial Irwan Indraloka, Dwi Smaradahana Irfan Suliansyah Jerrystama Abipradja, Jerrystama Juwairiah Juwairiah Karuniawati, Fenty Kgs Ahmadi Khoiriyatuinnisa, Khoiriyatunnisa Kisdjamiatun RMD Lanang, Fransiskus Lisa Dwi Wulandari Lisyani S Lisyani Suratmo, Lisyani Maharani, Dias Mangaras Yanu Florestiyanto, Mangaras Yanu Markus Priharjanto, Markus Maya Putri Arumsari, Maya Putri Mona Nabilah, Mona Muhamad Yunus Muhammad Riski, Muhammad Nanda, Angelica Mega Ni Putu Aniek Mahayani, Ni Putu Aniek Niken Puruhita Noverita Rochsitasari, Noverita Nugroho, Febri Satrio Nurdiansyah Rudi, Nurdiansyah Oliver Samuel Simanjuntak Perwira, Rifki Pramono Sasongko Prasetyo, Muhammad Bagus Prasetyo, Nanda Isdian Putra, Ludfie Hardian Ragil Saptaningtyas, Ragil Rahmat Adeputra, Rahmat Ranny Wahyu Ningrat Ratna Wulan Sari Ratomesang, Richardo Pelipus Boru Rejeki, VG. Sri Riangsari, M.F. Devita Rifki Indra Perwira Rifky Satya Wicaksono, Rifky Satya Rini Megawati Rizalditya, Putra Bintang Rr. M.I. Retno Susilorini Rudi Theo Satrio, Rudi Theo Rusdiana, Endang Sihombing, Ezra Siti Aisah Sofi Fauziah, Sofi Sofyan Cholid, Sofyan Sri Darmawati Sri Subanti Stefanus Eko Wiratno, Stefanus Sugiyono Sugiyono Suhartono Suhartono SUHARYANTO SUHARYANTO Suliana, Gatut Sumiyarti Sumiyarti, Sumiyarti Susilorini, Rr. M. I. Retno Susilorini, Rr.M.I Rretno Susilorini, Rr.M.I. Retno Tahta Alfina Tatty Ermin Taus, Dominika Sone Tiananda Widyarini Timba, Margaretha Kahi Umar Affandi Waenah . . Wahon, David Dolu Aman Wahyu Ari Widiyastuti, Wahyu Ari Wijiati, Irma Wilis Kaswidjanti Wirawan Wirawan Yan Indra Putra, Yan Indra Yoyon K. Suprapto Yoyon K. Suprapto Yuhefizar Yuhefizar Yurinda Amalia, Yurinda