p-Index From 2014 - 2019
9.997
P-Index
Articles

Found 2 Documents
Search
Journal : Majalah Kedokteran Bandung

Ekstrak Air Daun Padi Memperbaiki Hematopoesis pada Tikus yang Terpajan Plumbum

Majalah Kedokteran Bandung Vol 47, No 2 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar

Abstract

Plumbum (Pb) asetat menyebabkan gangguan hematopoesis melalui hambatan biosintesis heme dan defisiensi enzim G-6PD yang menimbulkan penurunan hemoglobin dan jumlah eritrosit, serta peningkatan sel retikulosit. Ekstrak daun padi mengandung protein metallothionein yang mengikat Pb. Tujuan penelitian adalah membuktikan ekstrak daun padi memperbaiki gangguan hematopoesis pada tikus terpajan Pb. Metode penelitian adalah randomized post test only control-group design, 28 tikus dibagi ke dalam 4 kelompok yaitu 1 kelompok kontrol dan 3 kelompok perlakuan. Kelompok kontrol dan perlakuan dipajan Pb 0,5 g/kgBB/hari dan kelompok perlakuan diberi ekstrak daun padi 0,2; 0,4; 0,8 setiap hari melalui sonde sampai minggu ke-8. Hari terakhir  minggu ke-8 diperiksa jumlah kadar Hb menggunakan cyanmethemoglobin dan jumlah eritrosit diukur menggunakan haematology analyzer, serta retikulosit menggunakan pengecatan BCB. Tempat penelitian dilaksanakan di LPPT UGM dan laboratorium biomedik UNIMUS Semarang, Maret–Mei 2014. Perbedaan antarkelompok untuk kadar Hb dan jumlah eritrosit dilakukan uji one way enzyme linked immunosorbent assays (ANOVA) dan Kruskal-Wallis untuk jumlah retikulosit. Hasil penelitian menunjukkan kadar Hb rata-rata mengalami kenaikan tidak bermakna (p=0.81), jumlah eritrosit rata-rata tidak ada perbedaan antara kelompok kontrol dan perlakuan (p=0,83), jumlah retikulosit rata-rata menurun bermakna dari kelompok kontrol sampai perlakuan (p=0,00). Simpulan, ekstrak daun padi mampu memperbaiki hematopoesis pada tikus terpajan Pb. [MKB. 2015;47(2):84–90]Kata kunci: Ekstrak daun padi, hematopoesis, pajanan Pb, tikusRice Leaf Water Extract Improves Hematopoiesis in Lead-Exposed Rats  Abstract Plumbum ( Pb ) acetate leads to hematopoiesis disruption by inhibiting heme biosynthesis and creating G-6PD enzyme deficiency, leading to decreased hemoglobin concentration and red cell count as well as  increased reticulocyte cells. Rice leaf extract contains metallothionein proteins that bind Pb. This study aimed to prove the ability of rice leaf extract wa to improve hematopoiesis disorder conditions in rats exposed to Pb. The method used was the randomized post-test only control group design. Twenty eight rats were divided into 4 groups: 1 control group and 3 treatment groups . In the control group and the treatments groups, the experimental rats were treated with 0.5 g/kg/day Pb. The treatment groups were treated by 0.2, 0.4, and 0.8 rice leaf extract, respectively, every day for 8 weeks. On the last day of the 8th week, blood samples were collected for Hb concentration measurements using cyanmethemoglobin and the erythrocytes and reticulocytes number were counted using a hematology analyzer and BCB, respectively. This study was conducted in LPPT UGM and Biomedicine UNIMUS in March–May 2014. The differences between groups in hemoglobin and erythrocyte parameters were tasted  using one way ANOVA while  Kruskal-Wallis test was used for the reticulocyte count. The results show that rice leaf extract treatment to rats exposed to Pb did not affect Hb (p=0.81) and erythrocytes count  (p=0.83), However, the mean reticulocyte count decreased significantly in Pb-exposed rats treated with rice leaf extract (p=0.00). In conclusion, rice leaf extract is able to improve hematopoiesis in rats exposed to Pb. [MKB. 2015;47(2):84–90]Key words: Hematopoiesis, Pb, rice leaf extract, rat DOI: 10.15395/mkb.v47n2.458

Ekstrak Air Daun Padi Memperbaiki Hematopoesis pada Tikus yang Terpajan Plumbum

Majalah Kedokteran Bandung Vol 47, No 2 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar

Abstract

Plumbum (Pb) asetat menyebabkan gangguan hematopoesis melalui hambatan biosintesis heme dan defisiensi enzim G-6PD yang menimbulkan penurunan hemoglobin dan jumlah eritrosit, serta peningkatan sel retikulosit. Ekstrak daun padi mengandung protein metallothionein yang mengikat Pb. Tujuan penelitian adalah membuktikan ekstrak daun padi memperbaiki gangguan hematopoesis pada tikus terpajan Pb. Metode penelitian adalah randomized post test only control-group design, 28 tikus dibagi ke dalam 4 kelompok yaitu 1 kelompok kontrol dan 3 kelompok perlakuan. Kelompok kontrol dan perlakuan dipajan Pb 0,5 g/kgBB/hari dan kelompok perlakuan diberi ekstrak daun padi 0,2; 0,4; 0,8 setiap hari melalui sonde sampai minggu ke-8. Hari terakhir  minggu ke-8 diperiksa jumlah kadar Hb menggunakan cyanmethemoglobin dan jumlah eritrosit diukur menggunakan haematology analyzer, serta retikulosit menggunakan pengecatan BCB. Tempat penelitian dilaksanakan di LPPT UGM dan laboratorium biomedik UNIMUS Semarang, Maret–Mei 2014. Perbedaan antarkelompok untuk kadar Hb dan jumlah eritrosit dilakukan uji one way enzyme linked immunosorbent assays (ANOVA) dan Kruskal-Wallis untuk jumlah retikulosit. Hasil penelitian menunjukkan kadar Hb rata-rata mengalami kenaikan tidak bermakna (p=0.81), jumlah eritrosit rata-rata tidak ada perbedaan antara kelompok kontrol dan perlakuan (p=0,83), jumlah retikulosit rata-rata menurun bermakna dari kelompok kontrol sampai perlakuan (p=0,00). Simpulan, ekstrak daun padi mampu memperbaiki hematopoesis pada tikus terpajan Pb. [MKB. 2015;47(2):84–90]Kata kunci: Ekstrak daun padi, hematopoesis, pajanan Pb, tikusRice Leaf Water Extract Improves Hematopoiesis in Lead-Exposed Rats  Abstract Plumbum ( Pb ) acetate leads to hematopoiesis disruption by inhibiting heme biosynthesis and creating G-6PD enzyme deficiency, leading to decreased hemoglobin concentration and red cell count as well as  increased reticulocyte cells. Rice leaf extract contains metallothionein proteins that bind Pb. This study aimed to prove the ability of rice leaf extract wa to improve hematopoiesis disorder conditions in rats exposed to Pb. The method used was the randomized post-test only control group design. Twenty eight rats were divided into 4 groups: 1 control group and 3 treatment groups . In the control group and the treatments groups, the experimental rats were treated with 0.5 g/kg/day Pb. The treatment groups were treated by 0.2, 0.4, and 0.8 rice leaf extract, respectively, every day for 8 weeks. On the last day of the 8th week, blood samples were collected for Hb concentration measurements using cyanmethemoglobin and the erythrocytes and reticulocytes number were counted using a hematology analyzer and BCB, respectively. This study was conducted in LPPT UGM and Biomedicine UNIMUS in March–May 2014. The differences between groups in hemoglobin and erythrocyte parameters were tasted  using one way ANOVA while  Kruskal-Wallis test was used for the reticulocyte count. The results show that rice leaf extract treatment to rats exposed to Pb did not affect Hb (p=0.81) and erythrocytes count  (p=0.83), However, the mean reticulocyte count decreased significantly in Pb-exposed rats treated with rice leaf extract (p=0.00). In conclusion, rice leaf extract is able to improve hematopoiesis in rats exposed to Pb. [MKB. 2015;47(2):84–90]Key words: Hematopoiesis, Pb, rice leaf extract, rat DOI: 10.15395/mkb.v47n2.458

Co-Authors Abdurrahman, Ahmad Fauzan Abiyoga, Gerald Arsa Adhiyaksa Ade Lia D. Safitri Adrianus Yosep Ago, Adrianus Yosep Agus Sasmito Aribowo Ahmad Warsun Ali Ridho Barakbah Andri S, Andri Andri Sukeksi Anin, Vitry Agrida Anissa Wulandari, Anissa Antariksa Antariksa Ari Yanuar Ridwan, Ari Yanuar Ari Yanuar, Ari Bambang Utoyo Boyke Frahmana, Boyke Budi Sulistyo Budianto, Anwar Budiyono Budiyono Buru, Romika Jibrael Dalin, Yulianus Dani Satyawan Deniswara, Cindy Dessyanto Boedi P Destaria Madya Verawaty, Destaria Madya Dida Diah Damayanti Didi Setya, Didi Didik Sumanto Dinda Sekar Putri, Dinda Sekar Djoko Legono Dwi Sulistyoningsih Dwiska Aini Nurrahma, Dwiska Aini Eka Fitasari Eka Puji Hastuti, Eka Puji Ekawati, Fitri Eko Prasetyo Nugroho Endang Purwaningsih Erik Kurniawan, Erik Ethica, Stalls Norma Fajar Kurnia Fani Yayuk Supomo Fauzia Hulopi Gita Purnama Sari, Gita Purnama Hairurraziqin Hairurraziqin, Hairurraziqin Hananta, Yan’s Dianaga Hani Purnamasari, Hani Hariyadi Hariyadi Hariyana, Bambang Helmi Helmi Henna Ria Sunoko, Henna Ria Henna Rya Sunoko Henna Rya Sunoko Herry Sofyan Hertanto Wahyu Subagio Hertanto Wahyu Subagyo Heru Cahya Rustamaji I Ketut Eddy P, I Ketut I Ketut Eddy P. I Made Tasma Ial Irwan Arahman, Ial Irwan Indraloka, Dwi Smaradahana Irfan Suliansyah Jerrystama Abipradja, Jerrystama Juwairiah Juwairiah Karuniawati, Fenty Kgs Ahmadi Khoiriyatuinnisa, Khoiriyatunnisa Kisdjamiatun RMD Lanang, Fransiskus Lisa Dwi Wulandari Lisyani S Lisyani Suratmo, Lisyani Maharani, Dias Mangaras Yanu Florestiyanto, Mangaras Yanu Markus Priharjanto, Markus Maya Putri Arumsari, Maya Putri Mona Nabilah, Mona Muhamad Yunus Muhammad Riski, Muhammad Nanda, Angelica Mega Ni Putu Aniek Mahayani, Ni Putu Aniek Niken Puruhita Njurumana, Yermias Yiwa Noverita Rochsitasari, Noverita Nugroho, Febri Satrio Nurdiansyah Rudi, Nurdiansyah Oliver Samuel Simanjuntak Panjang, Meha Tara Perwira, Rifki Pramono Sasongko Prasetyo, Muhammad Bagus Prasetyo, Nanda Isdian Putra, Ludfie Hardian Ragil Saptaningtyas, Ragil Rahmat Adeputra, Rahmat Rahmawati, Atina Ranny Wahyu Ningrat Ratna Wulan Sari Ratomesang, Richardo Pelipus Boru Rejeki, VG. Sri Riangsari, M.F. Devita Rifki Indra Perwira Rifky Satya Wicaksono, Rifky Satya Rini Megawati Rio Aurachman, Rio Rizalditya, Putra Bintang Rr. M.I. Retno Susilorini Rudi Theo Satrio, Rudi Theo Rusdiana, Endang Sihombing, Ezra Siti Aisah Sofi Fauziah, Sofi Sofyan Cholid, Sofyan Sri Darmawati Sri Subanti Stefanus Eko Wiratno, Stefanus Sugiyono Sugiyono Suhartono Suhartono SUHARYANTO SUHARYANTO Suliana, Gatut Sumiyarti Sumiyarti, Sumiyarti Susilorini, Rr. M. I. Retno Susilorini, Rr.M.I Rretno Susilorini, Rr.M.I. Retno Tahta Alfina Tantalu, Lorine Tatty Ermin Taus, Dominika Sone Tiananda Widyarini Timba, Margaretha Kahi Umar Affandi Veronika Veronika Waenah . . Wahon, David Dolu Aman Wahyu Ari Widiyastuti, Wahyu Ari Wijiati, Irma Wilis Kaswidjanti Wirawan Wirawan Yan Indra Putra, Yan Indra Yoyon K. Suprapto Yoyon K. Suprapto Yuhefizar Yuhefizar Yurinda Amalia, Yurinda