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Journal : International Journal of Science and Engineering (IJSE)

Zinc Supplementation Dosage Variations to Metallothionein Protein Level of Rattus Norvegicus

International Journal of Science and Engineering (IJSE) Vol 5, No 2 (2013)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (223.679 KB)

Abstract

Zinc is an essential trace element involving in the activity of more than 300 enzymes and proteins of human body. One important role of zinc is to improve metallothionein protein binding heavy metals and functioning as heavy metal detoxification facilities. This research discusses the effect of zinc supplement  on the  improvement of metallothionein protein level. The in vivo test involving 28 rats categorized in 4 groups was performed. The experiments used randomized post test control group design. The 3 groups were daily supplemented by zinc in th concentration of  0.2 mg, 0.4 mg, and 0.8 mg. Whereas, the last group was let without zinc treatment. As an indicator the metallothionein protein level was checked after three weeks. The data was then evaluated by Anova an Bonferroni test in order to know the signicant of protein level difference among the groups. The result showed that the average of metallothionein protein level improved by increasing zinc suppplement with the 0.95 ±0.20; 1.28 ±0.19; 1.39 ±0.09; 1.91 ±0.3 ng/ml metallothionein per 0, 0,2, 0,4, 0,8.mg zinc added. Based on the ANOVA and Bonferroni test,  indicated that the improvement was significant as shown with p value  of 0.00

THE ANALYSIS, IDENTIFICATION, AND FORMULATION OF METALLOTHIONEIN EXTRACT AVAILABLE IN ROOTS, STEMS, LEAVES , FLOWERS , AND GRAINS OF RICE, CORNS, BEANS , AND SOYBEANS

International Journal of Science and Engineering Vol 10, No 1 (2016)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (443.832 KB)

Abstract

Abstract –Vegetable materials are easily found around us but are frequently considered useless. The Metallothionein content in vegetable materials such in roots, stems, leaves, flowers, and fruit of rice, corns, beans, and soybeans has not been investigated before.  Metallothionein protein has the role to bind heavy metals and serves as means of detoxification of heavy metals. This studi investigated to analyze, identify, and formulate metallothionein extracts from vegetable materials such as in roots, stems, leaves, flowers, and fruit of rice, corns, beans, and soybeans. The Experimental, each vegetable material available in roots, stems, leaves, flowers and fruit of rice, corns, beans, and soybeans was made into simplisia (crude medication) ​​and then was processed into infuse. ELISA method was conducted as the measurement techniques upon protein level of each vegetable material infuse. The levels of Metallothionein protein available in vegetable materials were identified to determine the highest. The extract formula was taken from the highest levels of metallothionein. The result showed that the average metallothionein protein level from the entire vegetable materials was 0.62 ng: the highest in rice leaves of 1.4 ng and the lowest in bean flowers of 0.2 ng. The rice leaves were further formulated into metallothionein Extract. The analysis of metallothionein levels of vegetable materials such as in roots, stems, leaves, flowers, and fruit of rice, corns, beans and soybeans found that the highest level was in rice leaves of 1.354539 ng, and the lowest was in bean flowers of 0.22478.   Key Words - Vegetable, metallothionein..

Co-Authors Abdurrahman, Ahmad Fauzan Abiyoga, Gerald Arsa Adhiyaksa Ade Lia D. Safitri Adrianus Yosep Ago, Adrianus Yosep Agus Sasmito Aribowo Ahmad Warsun Ali Ridho Barakbah Andri S, Andri Andri Sukeksi Anin, Vitry Agrida Anissa Wulandari, Anissa Antariksa Antariksa Ari Yanuar Ridwan, Ari Yanuar Ari Yanuar, Ari Bambang Utoyo Boyke Frahmana, Boyke Budi Sulistyo Budianto, Anwar Budiyono Budiyono Buru, Romika Jibrael Dalin, Yulianus Dani Satyawan Deniswara, Cindy Dessyanto Boedi P Destaria Madya Verawaty, Destaria Madya Dida Diah Damayanti Didi Setya, Didi Didik Sumanto Dinda Sekar Putri, Dinda Sekar Djoko Legono Dwi Sulistyoningsih Dwiska Aini Nurrahma, Dwiska Aini Eka Fitasari Eka Puji Hastuti, Eka Puji Ekawati, Fitri Eko Prasetyo Nugroho Endang Purwaningsih Erik Kurniawan, Erik Ethica, Stalls Norma Fajar Kurnia Fani Yayuk Supomo Fauzia Hulopi Gita Purnama Sari, Gita Purnama Hairurraziqin Hairurraziqin, Hairurraziqin Hananta, Yan’s Dianaga Hani Purnamasari, Hani Hariyadi Hariyadi Hariyana, Bambang Helmi Helmi Henna Ria Sunoko, Henna Ria Henna Rya Sunoko Henna Rya Sunoko Herry Sofyan Hertanto Wahyu Subagio Hertanto Wahyu Subagyo Heru Cahya Rustamaji I Ketut Eddy P, I Ketut I Ketut Eddy P. I Made Tasma Ial Irwan Arahman, Ial Irwan Indraloka, Dwi Smaradahana Irfan Suliansyah Jerrystama Abipradja, Jerrystama Juwairiah Juwairiah Karuniawati, Fenty Kgs Ahmadi Khoiriyatuinnisa, Khoiriyatunnisa Kisdjamiatun RMD Lanang, Fransiskus Lisa Dwi Wulandari Lisyani S Lisyani Suratmo, Lisyani Maharani, Dias Mangaras Yanu Florestiyanto, Mangaras Yanu Markus Priharjanto, Markus Maya Putri Arumsari, Maya Putri Mona Nabilah, Mona Muhamad Yunus Muhammad Riski, Muhammad Nanda, Angelica Mega Ni Putu Aniek Mahayani, Ni Putu Aniek Niken Puruhita Njurumana, Yermias Yiwa Noverita Rochsitasari, Noverita Nugroho, Febri Satrio Nurdiansyah Rudi, Nurdiansyah Oliver Samuel Simanjuntak Panjang, Meha Tara Perwira, Rifki Pramono Sasongko Prasetyo, Muhammad Bagus Prasetyo, Nanda Isdian Putra, Ludfie Hardian Ragil Saptaningtyas, Ragil Rahmat Adeputra, Rahmat Rahmawati, Atina Ranny Wahyu Ningrat Ratna Wulan Sari Ratomesang, Richardo Pelipus Boru Rejeki, VG. Sri Riangsari, M.F. Devita Rifki Indra Perwira Rifky Satya Wicaksono, Rifky Satya Rini Megawati Rizalditya, Putra Bintang Rr. M.I. Retno Susilorini Rudi Theo Satrio, Rudi Theo Rusdiana, Endang Sihombing, Ezra Siti Aisah Sofi Fauziah, Sofi Sofyan Cholid, Sofyan Sri Darmawati Sri Subanti Stefanus Eko Wiratno, Stefanus Sugiyono Sugiyono Suhartono Suhartono SUHARYANTO SUHARYANTO Suliana, Gatut Sumiyarti Sumiyarti, Sumiyarti Susilorini, Rr. M. I. Retno Susilorini, Rr.M.I Rretno Susilorini, Rr.M.I. Retno Tahta Alfina Tantalu, Lorine Tatty Ermin Taus, Dominika Sone Tiananda Widyarini Timba, Margaretha Kahi Umar Affandi Veronika Veronika Waenah . . Wahon, David Dolu Aman Wahyu Ari Widiyastuti, Wahyu Ari Wijiati, Irma Wilis Kaswidjanti Wirawan Wirawan Yan Indra Putra, Yan Indra Yoyon K. Suprapto Yoyon K. Suprapto Yuhefizar Yuhefizar Yurinda Amalia, Yurinda