Yulvian Sani
Department of Pathology, Indonesian Research Center for Veterinary Science

Published : 29 Documents
Articles

Found 29 Documents
Search

Pesticide residues in brain tissues of dairy cattle in Lembang ., Indraningsih; Sani, Yulvian
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 1 (2006)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.804 KB) | DOI: 10.14334/jitv.v11i1.510

Abstract

The use of pesticides to control plant diseases may cause residual formation in crops, its byproduct and environmental. Furthermore, the use of agriculture byproduct as animal feed may cause poisoning or residual formation in animal products. The purpose of this study is to investigate of pesticide residues in brain tissues of dairy cattle in relation to animal feed as a contamination source. Samples consisted of animal feeds (19 samples of fodder and 6 samples of feed), 31 samples of sera and 25 samples of brain tissues of dairy cattle collected from Lembang, West Java. Feeds and fodders were collected from dairy farms located in Lembang. Sera were directly collected from 31 heads of Frisien Holstein (FH) cattle from the same location, while brain tissues of FH cattle were collected from a local animal slaughtering house. Pesticide residues were analysed using gas chromatography (GC). Both residues of organochlorines and organophosphates were detected from brain tissues with average residue concentration OP was 22.7 ppb and OC was 5.1 ppb and a total residue was 27.8 ppb. The pesticide residues in brain tissues are new information that should be taken into consideration since the Indonesian consumed this tissues as an oval. Although pesticides residue concentration was low, pathological changes were noted microscopically from the brain tissues including extracellular vacuolisation, focal necrosis, haemorrhages, dilatation of basement membrane without cellular infiltration. Both pesticide residues were also detected in sera, where OP (9.0 ppb) was higher than OC (4.9 ppb). These pesticides were also detected in animal feeds consisting fodders and feeds. Residues of OP (12.0 ppb) were higher than OC (1.8 ppb) in feeds, but residues of OP (16.8 ppb) were lower than OC (18.7 ppb) in fodders. Although, pesticide residues in sera and brain tissues were below the maximum residue limits (MRL) of fat, the presence of pesticides in brain tissues should be taken into consideration as their effects in brain lesions. There was a correlation between contaminant found in animal feeds and pesticide residues in sera and brain tissues of dairy cattle. Key Words: Pesticides, Residues, Brain, Dairy Cattle
Animal Health and Productivity Status of Cattle After The Eruption of Mount Merapi Sani, Yulvian; Estuningsih, E S
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 4 (2011): DECEMBER 2011
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.475 KB) | DOI: 10.14334/wartazoa.v21i4.963

Abstract

The eruption of Merapi from October 26th to November 6th, 2010 has affected social life and environment around the Merapi. The eruption has caused destruction of land and water resources, plants, death of animals and human casualities. The lava, dust and stones released from the eruption of Merapi had caused residential destruction, casualities, agricultural land and plants destruction, and contamination of water. The eruption has directly affected 4 districts including Sleman (Yogyakarta), Magelang, Boyolali and Klaten (Central Java) categorized as Disaster Risk Area (DRA). The purpose of this assessment is to analyse the impacts of Merapi eruption in animal health and productivity in particular for dairy and beef cattle. A total of 2.828 heads of cattle was reported died during the eruption of Merapi, and 1.962 heads died at the time of eruption and 36 heads at the arrival on evacuation areas. Animal that found died including 423 heads of beef cattle (0.13%) and 2.405 heads of dairy cattle (3.2%). Clinical sains noted after the eruption were reduction of milk production, loss of appetite, diarrhoea, respiratory disturbances, mastitis and collapse. The main problems for livestock were reduction of milk production, collapse of dairy milk corporation activities and contamination of water resources. Other than dairy cattle mortality, the reduction of milk production may be caused by subclinical mastitis and environmental distress due to temperature and noise of eruption for few days. The subclinical mastitis should be further investigated to establish rehabilitation programme for dairy milk agribussiness activity in particular around the DRA of Merapi. Key words: Health, productivity, cattle, disaster, Merapi
Factors Affecting Food Safety of Animal Origin in Indonesia Bahri, Sjamsul; Sani, Yulvian; ., Indraningsih
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 1 (2006): MARCH 2006
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1154.386 KB) | DOI: 10.14334/wartazoa.v16i1.864

Abstract

Based on the Indonesian Regulation Number 7 year 1996, food is defined as everything derived from biological sources and water, either processed and non-processed materials to be used as food and drinks, including food additives, food raw materials and other materials for preparation process, processing and production of food or drinks . Furthermore, food safety is a condition and an approach required to prevent the food from contamination of pathogenic microbes, toxic compounds and other xenobiotics that may affect and hazardous to human health . Food safety is basically a complexity and close related to policy . toxicity, microbiology, chemicals, nutrition status, health and public welfare . On the other hand, food safety problem is a dynamic process following the changing of public society including socioculture, health, development of science and technology as well as everything related to human life . In general, there are three main stages of process as the critical point in food safety of animal origin, namely : (1) preharvest ; (2) production ; and (3) postharvest . The main factors of these stages are discussed in this paper .   Key words : Food safety, livestock, preharvest, harvest, post harvest
Detection of dioxins Trichloro dibenzo-p-dioxins and Trichloro dibenzofurans in beef with Gas Chromatography Tandem Mass Spectrometry ., Indraningsih .; Sani, Yulvian
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 4 (2014): DECEMBER 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.383 KB) | DOI: 10.14334/jitv.v19i4.1098

Abstract

This study is to investigate the residue level of TCDDs/TCDFs in beef collected from Animal Slaughter Houses in Giwangan (Yogyakarta), Klaten (Central Java) and Kupang (East Nusatenggara). Residue of persistent organic pollutants (POPs) was also analysed with GC-ECD, and dioxins with GC-MSMS. Fifty samples were collected form Giwangan (20), Klaten (15) and Kupang (15). Results shows that POPs were detected in beef samples including aldrin, dieldrin, DDT, endrin and heptachlor. The highest total residues of POPs were detected in beef from Giwangan (Yogyakarta) at 93.11 hg/g, followed by Klaten (17.79 hg/g) and Kupang (12.87 hg/g). Residues of POPs were below the maximum residue limit stated by the SNI 7313: 2008. Average of total TEQ for TCDDs/TCDFs in beef from Giwangan was 13,624.38 rg/g with a range between 4,496.66 to 20,642.40 rg/g was higher than that from Kupang at 1,623.98 rg/g with a range between 0.83 to 6,471.07 rg/g. On the other hand, dioxins were not detected in beef from Klaten. The total TEQ of TCDDs/TCDFs in beef samples from Giwangan was 54,497.52 rg/g and Kupang was 6,495.9 rg/g. These results indicated that there was an association between the presence of endrin and heptachlor with the presence of TCDDs/TCDFs residues in beef that this is may be new information for further studied.
Pathological and biochemical changes in intoxication of mindi (Melia azedarach) leaf Sani, Yulvian; Bahri, Sjamsul
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 2 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (729.221 KB) | DOI: 10.14334/jitv.v1i2.24

Abstract

The purpose of this study was to investigate pathological and biochemical changes in intoxication of mindi leaf (Melia azedarach) on skeletal muscle and myocardial tissues . Fifty two male Wistar rats weighing 140 g to 240 g were intoxicated in 20%; 25% and 40% diet of mindi leaf ad libitwn for 25 days . Clinical signs did not appear the intoxication of mindi leaf. Specific pathological changes were not found macroscopically except cachexia and paleness . Skeletal muscle lesions on the other hand, were consistently noted microscopically . There were degeneration, necrosis and fragmentation of muscle fibres ; enlargement of nuclei ; fibrosis; oedema and hyalination . The hyalination was prominent and appeared to be more. progressive two weeks after dosing . Following the skeletal muscle lesions, myocardial showed degeneration, necrosis and fragmentation of fibres ; enlargement of nuclei and hypercellularity . Regeneration of skeletal muscle developed on day-5 after the substitution of mindi diet by normal diet indicated by disappearing of hyalin tissues . But, myocardial appeared to be regenerated two days after the substitution of diet . Enzymatic activities of alanine aminotransferase (ALAI) and aspartate aminotransferase (ASAT) were not affected. Both enzymes in treated animals were lower than the control . The substitution of diet showed an increased of enzymatic activities during the first two weeks of feeding then reduced at the subsequent weeks . However, these enzymes increased on day-3 after substitution and appeared to maximum followed by a reduction of enzymatic activities in week-4 . On the other hand, creatinine phosphokinase (CPK) was affected in which higher level was noted in treated groups than control . Substitution of diet has led to the reduction of cpc level on day-2 and appeared to reach the same level as in control group on day-21 . The control group was not affected either clinically, pathologically or biochemically . It remained normal throughout the experimental period .
EVALUATION OF FARMERS APPRECIATION IN REDUCING PESTICIDE BY ORGANIC FARMING PRACTICE ., Indraningsih; Sani, Yulvian; Widiastuti, Raphaella
Indonesian Journal of Agricultural Science Vol 6, No 2 (2005): October 2005
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vegetables and dairy milk are important commodities in Pangalenganand Lembang, West Java. However, agrochemicals areused intensively and excessively in production system. Therefore,pesticide residues and contamination commonly occurredin agricultural products and environments. The study aimed toassess farmers’ attitudes on pesticide toxicity and reducingpesticide residues in animal and food crops products, andinvestigate the attitude changes of farmers on pesticide use. Itwas an on-farm research and farmers were directly involved inthe study. The attitude change was analyzed on questionnaireand interview base for over 99 respondents in Pangalengan andLembang between 2001 and 2003. Samples of soils, weeds,cabbages and milk were collected for pesticide residue analysis.Results showed that farmers did not aware on toxicity effectsof pesticides in both animal and human health. There wasmisinterpretation among the farmers where pesticides wereregarded as drugs rather than toxic compound to increaseproductivity. The organochlorines/OCs (lindane and heptachlor)were common pesticide contaminants found in soils of 7.9-11.4 ppb, but no organophospates (OPs) were detected. BothOCs and OPs were also detected in soils of Lembang at a rangeof 11.53-65.63 ppb and 0.6-2.6 ppb, respectively. There werepesticide residues detected in weeds collected from Pangalengan(8.93 ppb lindane, 2.05 ppb heptachlor, and 33.27 ppbchlorpyriphos methyl/CPM and Lembang (6.45 ppb lindane,2.65 ppb endosulfan, 6.85 ppb diazinon, and 0.5 ppb CPM).Only endosulfan with least residue level (0.1 ppb) was detectedin organic cabbages, whereas lindane was detected much higher(3.7 ppb) in non-organic cabbages. Pesticide residues were notdetected in milk of dairy cattle fed on by-products of organiccabbages, but lindane was still present in milk of dairy cattle fedon non-organic cabbages for 7 days subsequently. The presentstudy indicates that the organic farming practice may reducepesticide residues in animal and agricultural products. Farmersappreciated that pesticides may cause residual effects on theirproducts (95.2%) and affected public health and environment(92.9%). Therefore, they were willing to convert theiragricultural practices to organic farming (69.1%).
Pathological and biochemical changes in intoxication of mindi (Melia azedarach) leaf Sani, Yulvian; Bahri, Sjamsul
Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 2 (1995)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (729.221 KB) | DOI: 10.14334/jitv.v1i2.24

Abstract

The purpose of this study was to investigate pathological and biochemical changes in intoxication of mindi leaf (Melia azedarach) on skeletal muscle and myocardial tissues . Fifty two male Wistar rats weighing 140 g to 240 g were intoxicated in 20%; 25% and 40% diet of mindi leaf ad libitwn for 25 days . Clinical signs did not appear the intoxication of mindi leaf. Specific pathological changes were not found macroscopically except cachexia and paleness . Skeletal muscle lesions on the other hand, were consistently noted microscopically . There were degeneration, necrosis and fragmentation of muscle fibres ; enlargement of nuclei ; fibrosis; oedema and hyalination . The hyalination was prominent and appeared to be more. progressive two weeks after dosing . Following the skeletal muscle lesions, myocardial showed degeneration, necrosis and fragmentation of fibres ; enlargement of nuclei and hypercellularity . Regeneration of skeletal muscle developed on day-5 after the substitution of mindi diet by normal diet indicated by disappearing of hyalin tissues . But, myocardial appeared to be regenerated two days after the substitution of diet . Enzymatic activities of alanine aminotransferase (ALAI) and aspartate aminotransferase (ASAT) were not affected. Both enzymes in treated animals were lower than the control . The substitution of diet showed an increased of enzymatic activities during the first two weeks of feeding then reduced at the subsequent weeks . However, these enzymes increased on day-3 after substitution and appeared to maximum followed by a reduction of enzymatic activities in week-4 . On the other hand, creatinine phosphokinase (CPK) was affected in which higher level was noted in treated groups than control . Substitution of diet has led to the reduction of cpc level on day-2 and appeared to reach the same level as in control group on day-21 . The control group was not affected either clinically, pathologically or biochemically . It remained normal throughout the experimental period . Key words : Melia azedarach, intoxication, pathology, biochemistry, skeletal muscle, myocardial
Factors Affecting Food Safety of Animal Origin in Indonesia Bahri, Sjamsul; Sani, Yulvian; ., Indraningsih
Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 1 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1154.386 KB) | DOI: 10.14334/wartazoa.v16i1.864

Abstract

Based on the Indonesian Regulation Number 7 year 1996, food is defined as everything derived from biological sources and water, either processed and non-processed materials to be used as food and drinks, including food additives, food raw materials and other materials for preparation process, processing and production of food or drinks . Furthermore, food safety is a condition and an approach required to prevent the food from contamination of pathogenic microbes, toxic compounds and other xenobiotics that may affect and hazardous to human health . Food safety is basically a complexity and close related to policy . toxicity, microbiology, chemicals, nutrition status, health and public welfare . On the other hand, food safety problem is a dynamic process following the changing of public society including socioculture, health, development of science and technology as well as everything related to human life . In general, there are three main stages of process as the critical point in food safety of animal origin, namely : (1) preharvest ; (2) production ; and (3) postharvest . The main factors of these stages are discussed in this paper .   Key words : Food safety, livestock, preharvest, harvest, post harvest
Animal Health and Productivity Status of Cattle After The Eruption of Mount Merapi Sani, Yulvian; Estuningsih, E S
Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 4 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.475 KB) | DOI: 10.14334/wartazoa.v21i4.963

Abstract

The eruption of Merapi from October 26th to November 6th, 2010 has affected social life and environment around the Merapi. The eruption has caused destruction of land and water resources, plants, death of animals and human casualities. The lava, dust and stones released from the eruption of Merapi had caused residential destruction, casualities, agricultural land and plants destruction, and contamination of water. The eruption has directly affected 4 districts including Sleman (Yogyakarta), Magelang, Boyolali and Klaten (Central Java) categorized as Disaster Risk Area (DRA). The purpose of this assessment is to analyse the impacts of Merapi eruption in animal health and productivity in particular for dairy and beef cattle. A total of 2.828 heads of cattle was reported died during the eruption of Merapi, and 1.962 heads died at the time of eruption and 36 heads at the arrival on evacuation areas. Animal that found died including 423 heads of beef cattle (0.13%) and 2.405 heads of dairy cattle (3.2%). Clinical sains noted after the eruption were reduction of milk production, loss of appetite, diarrhoea, respiratory disturbances, mastitis and collapse. The main problems for livestock were reduction of milk production, collapse of dairy milk corporation activities and contamination of water resources. Other than dairy cattle mortality, the reduction of milk production may be caused by subclinical mastitis and environmental distress due to temperature and noise of eruption for few days. The subclinical mastitis should be further investigated to establish rehabilitation programme for dairy milk agribussiness activity in particular around the DRA of Merapi. Key words: Health, productivity, cattle, disaster, Merapi
The effectiveness of garlic extract (Allium sativum linn) in controlling aflatoxicosis in laying hens Maryam, Romsyah; Sani, Yulvian; Juariah, Siti; Firmansyah, Rachmat; ., Miharja
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 4 (2003)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.496 KB) | DOI: 10.14334/jitv.v8i4.397

Abstract

Aflatoxicosis is a disease generated as the consequence of aflatoxin contamination in foods and feeds. Garlic (Allium sativum Linn.), a natural spice known to reduce the toxicity of aflatoxins in poultry. Twenty five laying hens were used to study the effectiveness of garlic extract in reducing aflatoxicosis. The animals were divided into 5 groups i.e (1) control group, (2) group treated with 0.4 mg/kg BH, (3) group treated with 0.4 mg/kg BH and 4% garlic extract in feed, (4) group treated with 5 mg/kg BH, and (5) group treated with 5.0 mg/kg BH and 4% garlic extract in feed. Body weight gains, eggs production, and the activity of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), and γ-glutamytransferase (γ- GT) enzymes were observed every week. The residue levels of the aflatoxin and metabolites were measured in the eggs using high performance liquid chromatography (HPLC). The results indicated that group treated with 0.4 mg AFB1/kg BH dan 5.0 mg AFB1/kg BH resulted in decreasing body weight gains, egg production, and increasing the level of GOT, GPT and γ-GT. The addition of 4% garlic extract in the feed was effective to improve the body weight gain and egg production only in the group of chicken treated with 0.4 mg AFB1/kg BH. However, it decreased the enzymes activities of the GOT, GPT, and γ-GT, as well as reduced the aflatoxin residues and metabolites in the groups at both aflatoxin levels (0.4 and 5.0 mg/kg BH). Aflatoxin residues decreased up to 42.2% for the group treated with the low dose of AFB1 (0.4 mg/kg) and 49.0% for the group treated with the high dose of AFB1 (5 mg/kg).   Key words: Garlic, aflatoxicosis, laying hens, aflatoxin residues