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KRIOPRESERVASI SEMEN DOMBA GARUT DENGAN PENGENCER TRIS YANG DISUPLEMENTASI ETHYLENE DIAMINE TETRAACETIC ACID (CRYOPRESERVATION OF GARUT RAM SEMEN WITH TRIS EXTENDER ETHYLENEDIAMINETETRAACETIC ACID) Rizal, Muhammad; ., Herdis; ., Nasrullah; Riyadhi, Muhammad; Sangadji, Insun; ., Yulnawati
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Ethylene diamine tetraacetic acid (EDTA) is a molecular structure that binds to heavy metals andother toxins which needed to protect the spermatozoa during the cryopreservation process. The objectiveof this research was to examine EDTA in maintain the quality of garut ram frozen semen. Semen werecollected from mature Garut ram by artificial vagina. Fresh semen were evaluated and divided in equalvolume into three tubes and diluted with Tris extender containing 20% egg yolk (TEY-20) as control, TEY-20 + 0.01% EDTA (EDTA0.01), and TEY-20 + 0.02% EDTA (EDTA0.02), respectively. Semen was loaded in 0.25 mL mini straw with the concentration of 100 million motile spermatozoa. Semen was equilibrated at5oC for three hours, then frozen and stored in liquid nitrogen container. Quality of spermatozoa includingpercentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) wereevaluated after diluting, equilibrating, and thawing. Data were analyzed using completely randomizeddesign with three treatments and five replicates. Means were compared significant difference test at 0.05significant level. Results of this research showed that mean volume, color, consistency, pH, mass movement,spermatozoa concentration, percentage of MS, percentage of LS, percentage of abnormal spermatozoa,and percentage of IPM of garut ram fresh semen were 0.87 mL, cream, thick, 6.98, 3, 4,296 million cell/mL,75%, 86.33%, 4.4%, and 85%, respectively. Percentages of MS, LS, and IPM after thawing in control (42.5,52.5, and 52.17%) was not significantly difference with EDTA0.01 (44.17, 52.5, and 52.5%) and EDTA0.02(39.17%, 51%, and 48.33%). In conclusion, the addition of EDTA in Tris extender can maintain quality ofGarut ram frozen semen, and its suitable using in artificial insemination program. Addition of EDTA cannot enhancing quality of garut ram frozen semen.
DAYA HIDUP SPERMATOZOA EPIDIDIMIS KAMBING PERANAKAN ETTAWA YANG DIPRESERVASI DENGAN PENGENCER TRIS DAN BBERBAGAI KONSENTRASI MALTOSA Rizal, Muhammad; Sulistiowati, Dwi; Sulaiman, Abrani; Herdis, Herdis; Sangadji, Insun
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.086 KB) | DOI: 10.22146/jsv.22824

Abstract

Cauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, since the spermatozoa is motile and has ability for fertilizing the oocyte. Theobjective of this research was to examine the effectivity of maltose in maintaining viability of ettawa crossbreed goat epididymal spermatozoa preserved at 3?5oC. Five testis with epididymides of ettawa crossbreed goat were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure of cauda epididymides with physiological saline (0.9% NaCl). Collected-spermatozoa wasdivided in equal volume into three tubes and diluted with Tris extender containing 20% egg yolk (control), Tris extender + 0.3 g maltose/100 ml (M0.3), and Tris extender + 0.6 g maltose/100 ml (M0.6), respectively. Dilutedspermatozoa was stored in refrigerator at 3?5oC. Quality of diluted-spermatozoa including percentages of motile spermatozoa (MS) and live spermatozoa (LS) were evaluated every day during storage at 3?5oC for four days. Data were analyzed using completely randomized design with three treatments and five replicates. Means were compared significant difference test at 0.05 significant level. Results of this study showed that mean spermatozoaconcentration, percentage of MS, percentage of LS, and percentage of abnormal spermatozoa of ettawa crossbreed goat fresh epididymal spermatozoa were 3,220 million cell/ml, 70%, 81%, and 4.3%, respectively. At day-5 of storage, percentages of MS and LS for M0.3 (38 and 60.4%) and M0.6 (38 and 57.2%) were significantly (P<0.05) higher than control (32 and 55.4%). In conclusion, addition of 0.3 and 0.6% maltose in Tris extender could be maintained viability of ettawa crossbreed goat epididymal spermatozoa preserved at 3?5oC forthree days.
LAMA PENYIMPANAN DAGING SAPI TERHADAP ALT BAKTERI Sangadji, Insun
Biosel: Biologi Science and Education Vol 2, No 1 (2013): BIOLOGY SCIENCE AND EDUCATION
Publisher : IAIN Ambon

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Abstract

Penelitian dilaksanakan di Laboratorium MIPA Institut Agama Islam Negeri (IAIN) Ambon pada tanggal 15 April sampai 22 April 2013. Analisis data yang digunakan adalah kuantitatif dengan menggunakan uji ANOVA. Hasil penelitian inferensial dengan menggunakan uji ANOVA pada efek lama penyimpanan daging sapi terhadap total koloni bakteri, pada penyimpanan 0 hari memiliki nilai rata-rata 2353, 25 x 107/gram daging sapi, sedangkan penyimpanan 1 hari memiliki nilai rata-rata 6,53 x 105, penyimpanan 2 hari nilai rata-rata 4,50 x 104/gram daging sapi, penyimpanan 3 hari nilai rata-rata 1,36 x 104/gram daging sapi. Ini menandakan daging semakin lama penyimpanan jumlah koloni bakteri makin sedikit karena resiko terkontaminasi daging biasanya berasal dari (RPH) rumah potong dan para pekerja. Data hasil penelitian dihitung dengan menggunakan uji-F, diperoleh nilai F hitung = 10,06, dan F, table 5% = 3,59 dan 1 % = 6,22, sehingga H0 ditolak karna perlakuan memberikan berpengaruh terhadap variabel respons. Kata Kunci: Daging Sapi, Lama Penyimpanan, Koloni Bakteri
KANDUNGAN ANTOSIANIN DI DALAM MAHKOTA BUNGA BEBERAPA TANAMAN HIAS Sangadji, Insun; Rijal, Muhammad; Kusuma, Yulia Astri
Biosel: Biologi Science and Education Vol 6, No 2 (2017): BIOLOGY SCIENCE AND EDUCATION
Publisher : IAIN Ambon

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Abstract

Penelitian ini menggunakan Rancangan Acak lengkap yang terdiri dari 4 perlakuan (jenis mahkota bunga) dan tiap perlakuan diulang sebanyak 4 kali, sehingga total pengamatan yaitu 16 unit pengamatan. Berdasarkan hasil penelitian dan pembahasan, maka dapat disimpulkan bahwa terdapat kandungan antosianin yaitu pelargonidin dan sianin serta perbedaan kadar antosianin di dalam bunga mawar (Rosa sherardi), kembang sepatu (Hibiscus rosa sinensis L), pukul empat (Mirabilis jalapa L.), dan rosella (Hibiscus sabdariffa L.), disebabkan beberapa faktor yaitu cahaya matahari, iklim, dan tanah dengan kadar tertinggi terdapat pada bunga pukul empat, yakni dilihat pada penyelesaian Anova Kadar antosianin signifikan karena, perlakuan dari empat jenis mahkota bunga berpengaruh nyata terhadap kadar antosianin sebagai pewarna alami. Kata Kunci: Antosianin, Mahkota Bunga, Pewarna Alami
Lama Penyimpanan Daging Ayam Broiler Terhadap Kualitasnya Ditinjau Dari Kadar Protein Dan Angka Lempeng Total Bakteri Sangadji, Insun; Jurianto, Jurianto; Rijal, Muhammad
Biosel (Biology Science and Education): Jurnal Penelitian Sains dan Pendidikan Vol 8, No 1 (2019): BIOSEL (BIOLOGY SCIENCE AND EDUCATION): JURNAL PENELITIAN SCIENCE DAN PENDIDIKA
Publisher : IAIN Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v8i1.846

Abstract

This study aims to determine the effect of storage time of broiler chicken on its quality in terms of protein content and total bacterial plate number. This research was conducted on 11-19 December 2017. The design used in this study was a Completely Randomized Design (CRD) with 3 treatments consisting of 2 days of storage (A1), 4 days (A2) and 6 days (A3) and the number of replications is 3 replications. Data were analyzed using ANOVA. Sample analysis was carried out in the MIPA laboratory at the Faculty of Tarbiyah and Teacher Training, Ambon IAIN and Pattimura University Basic Chemistry Laboratory. The results showed that there was an effect of the length of storage of broiler chicken on protein levels and total bacterial plate numbers. The effect of broiler chicken meat storage on protein content was 14.2% while the effect of broiler chicken storage on the total plate number of bacteria was 12.7%. Key Words: Duration of Storage, Broiler Chicken Meat, Protein Levels, ALT