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Journal : Indonesian Journal of Fundamental and Applied Chemistry

The Effect of Lactobacillus acidophilus and Chito−Oligosaccharide on Antibacterial Activity and Organic Acid Production Miksusanti, Miksusanti; Saputra, Harian; Sandi, Sofia; Hermansyah, Hermansyah
Indonesian Journal of Fundamental and Applied Chemistry Vol 1, No 2 (2016): June 2016
Publisher : Indonesian Journal of Fundamental and Applied Chemistry

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Abstract

The effect of Lactobacillus acidophilus combined with and without Chito-Oligosaccharide (COS) on the growth of Escherichia coli and Staphylococcus aureus had been studied. The antibacterial activity of L. acidophilus before and after combination with COS 0.2% was tested on bacteria of E. coli and S. aureus with well-diffusion method. Incubation time was carried out in 44, 46, 48, 50, and 52 hours. Organic acids produced by L. acidophilus bacteria was measured by HPLC. The result showed that COS 0.2% can inhibit the growth of E. coli and S. aureus 37.2 mm2 and 52 mm2 respectively. Combination of L. acidophilus and COS 0.2% gave inhibition zone larger than L. acidophilus without COS 0.2%. Incubation time within 48 hours of L. acidophilus combined with COS 0.2% produced the largest inhibition zone against E. coli and S. aureus 367.92 mm2 and 343.99 mm2 respectively. Optical density measurement resulted in higher value for combination one but concentration of organic acid produced was lower compare to L. acidophilus without COS 0.2%.Keywords: COS, L. acidophilus, Organic acid, antibacterial.
The Effect of Fermentation Bran and Chitosan in Ration to Percentage of Tegal Duck Digestive Tract Weight Sahara, Eli; Sandi, Sofia; Yossi, Fitra
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 4, No 1 (2019): February 2019
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

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Abstract

Duck productivity is largely determined by the optimization of bodily functions. The food consumed greatly determines the development of digestive organs and internal organs of livestock. Digestive organs that are well developed and function optimally will be very decisive in turning feed into meat and eggs. Chitosan is a crustacean waste product known as animal fiber and is antimicrobial. Whereas fermented bran contains natural fiber rich in nutrients and is hypocholesterol. The aim of the study was to look at the role of chitosan and bran fermented on the weight percentage of digestive organs of ducks. The study used a completely randomized design (CRD) with 4 treatments and 5 replications. Each test consists of 2 ducks as a unit of experiment. The treatment is R0 = 45% corn + 35% concentrate + 20% bran without fermentation, R1 = 45% corn + 35% concentrate + 20% fermented bran, R2 = 45% corn + 35% concentrate + 19.5% fermented bran + 0.5% chitosan and R3 = 45% corn + 35% concentrate + 17.5% fermented + 2.5% chitosan bran. The variable measured is the weight percentage of the digestive organs (gizzard, small intestine and pancreas) in ducks. Data was processed using SAS Windows 16. The results showed that the administration of fermented chitosan and bran gave an average percentage of gizzard weight (ventriculus) and pancreatic weight percentage was not significantly different between treatments (P> 0.05), while treatment R1 (20% fermented bran) It was shown that the percentage of small intestine weight was higher (31.85%) than control R0.
Acid Resistance Test of Probiotic Isolated from Silage Forage Swamp on In Vitro Digestive Tract Sandi, Sofia; Miksusanti, Miksusanti; Sari, Meisji Liana; Sahara, Eli; Supriyadi, Adi; Gofar, Nuni; Asmak, Asmak
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 4, No 1 (2019): February 2019
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

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Abstract

Antibiotics are commonly used in poultry ration, and afterward these drug residues may persist in foods. There is an alternative source of antibiotics in poultry rations, one of which is using probiotics. Probiotics are living microorganisms capable of reaching the gastrointestinal tract and benefiting health, leaving no residue in the body. Probiotics can be originated from bacteria such as lactic acid bacteria (LAB) which produced lactic acid and antimicrobial components. LAB must be able to live in the digestive system with various pH condition. pH resistance testing of LAB was performed by introducing diluted bacteria into several buffer solutions with different pH and a predetermined incubation time, then was grown in MRS agar medium. This study was conducted to determine the resistance of LAB isolates as probiotics from silage forage swamp at different pH distributed in vitro digestant. The design used was a complete randomized design consist of 3 treatments and 3 replications. The treatments were using isolate of Kumpai Tembaga silage (P1), isolate of 50% Kumpai Tembaga silage and 50% Kemon Air silage (P2), and isolate of Kemon Air silage (P3). The results showed that all isolates used were lactic acid bacteria, and the treatment significantly affected the value of LAB resistance test against low pH and high pH. Isolate from Kumpai Tembaga silage (P1) is a lactic acid bacterium with higher ability to survive in pH of in vitro digestive system.
Evaluation of the Use of Cassava Based Complete Ration Silage on Nitrogen Retention and Metabolizable Energy Male Duck Sandi, Sofia; Sudarman, Asep; Laconi, Erika B; Wiryawan, Komang G; Mangunwijaja, Djumali
Indonesian Journal of Fundamental and Applied Chemistry Vol 3, No 2 (2018): June 2018
Publisher : Indonesian Journal of Fundamental and Applied Chemistry

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Abstract

The purpose of this study was to evaluate the effect of a complete ration silage made of cassava biomass  nitrogen retention, and metabolizable energy male ducks. Control diet consisted of corn, rice bran, coconut meal, soybean meal, vegetable oil, fish meal and premix. While the treatment of silage ration of cassava based (BBS) consisted of leaves, peel, and tubers of cassava, as well as a mix of fish meal, vegetable oil, premix, DL-methionine and L-lysine. The experimental design used was completely randomized design with 5 treatments and 4 replicates ie S0 (100% control diet), S25 (75% control diet + 25% BBS silage), S50 (50% control diet + 50% BBS silage), S75 (25% control diet + 75% BBS silage), and S100 (100% BBS silage ration). Measurement of metabolizable energy and nitrogen retention was conducted using 25 male ducks aged 10 weeks maintained in metabolic cages and adapted to experimental diets for seven days. Ducks were fasted for 24 hours before the experimental diets were offered. Metabolizable energy and nitrogen retention were measured by the method of Sibbald (1989). The results showed that a decline in nitrogen retention and, metabolizable energy along with the increasing level of BBS silage in the rations. The conclusion of this study was the use of up to 75% BBS silage generated metabolizable energy and nitrogen retention that do not differ from the control ration.Key words: cassava silage, complete ration, male duck, metabolizable energy, nitrogen retention