Muhammad Ade Salim
Program Studi Peternakan, Fakultas Pertanian, Universitas Khairun

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PENGARUH PERBEDAAN KEMATANGAN AIR KELAPA HIJAU SEBAGAI BAHAN PENGENCER YANG DITAMBAH 10% KUNING TELUR TERHADAP KUALITAS SEMEN CAIR KAMBING BOER

TERNAK TROPIKA Journal of Tropical Animal Production Vol 18, No 1 (2017): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Abstract

This study is conducted on December 2016until January 2017 in Sumber Sekar Field Laboratory, Faculty of Animal Husbandry, Brawijaya University. The purpose of this study is to determine the effect of different maturity levels of green coconut water dilution with 10% of egg yolk to the sperm quality of Boer goat during storage. Materials used in the study were male Boer kept in Sumber Sekar Field Laboratory. The semen was collected routinely once in 2 days using artificial vagina with fresh semen quality requirement individual motility ≥70%. The method used in this study is Randomnized Block Design which consists of three treatments, there are P0 as control (CEP-2 + 10% Egg Yolk); P1 (Young Green Coconut Water + 10% Egg Yolk) and P2 (Mature Green Coconut Water + 10% Egg Yolk). If the difference between the treatments showed real influence, then the Duncan’s Multiple Range Test will be done. The grouping is sorted by the semen observation time. The observed variables macroscopically include volume, smell, consistency, color and acidity, and microscopically which include individual motility, mass motility, viability and abnormality.The study result for the mean and standard deviation (SD) of motility in storage until the 4th day. The motility of P0 (36.67 ± 32.96)%, then P1 (20.0 ± 13.78)%, and the last P3 (18.33 ± 19.15). Dilution with the best viabillity average in the 4th day in P0 (58.02 ± 19.93)%, in P1 (26.10 ± 18.47)% and the last is P2 (25,87 ± 20,60)%.the average value of abnormality on the 4th day shows that the lowest average abnormality found in P0 (1.49 ± 0.73)%, then P1 (1.89 ± 1.68)% and the highest average value of abnormality is in P2 (2,87 ± 1.41).Keywords : liquid semen, boer goat, green coconut water, CEP-2, cold storage

PENGARUH AIR KELAPA MERAH YANG MUDA DAN TUA SEBAGAI PENGENCER TERHADAP KUALITAS SEMEN KAMBING BOER SELAMA PENYIMPANAN DINGIN

TERNAK TROPIKA Journal of Tropical Animal Production Vol 18, No 1 (2017): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Abstract

The aim of this research was to know the effect of maturity of red coconut water as a diluent to the sperms quality of Boer goats during chilled preservation. The research was carried out at Laboratory Sumber Sekar Faculty of Animal  Husbandry University of Brawijaya Malang, on 11st December 2016 until 31st January 2017. The method used in this research was laboratory experimental using Randomized Block Design with three treatments, P0 (Tris Aminomethane + 20% Egg Yolk), P1 (Young Red Coconut Water + 20% Egg Yolk) and P2 (Mature Red Coconut Water + 20% Egg Yolk) which each treatment using 10 repetitions. The observed variables include the percentage of motility, percentage of viability and percentage of abnormal spermatozoa during chilled preservation The results showed that on the 3ndday the percentage of motility of spermatozoa during chilled preservation gave a significant effect (P<0.05), while on viability gave a very significant effect (P<0.01), and on abnormality did not give a significant effect (P> 0.05). The highest of total motile spermatozoa was P0 (Tris Aminomethan + 20% Egg Yolk). P2 better than P1 on motility, but not on viability and abnormality. The conclusions was the maturity of red coconut water can improve motility, but can not improve viability and abnormality. The suggested was use Mature Red coconut water + 20% Egg Yolk as a diluent  and do further research on coconut water to be able to maintain spermatozoa longer.Keyword : Sperms, egg yolk, red coconut water, tris aminomethane

PENGARUH PENGHILANGAN RAFINOSA DALAM PENGENCER TRIS AMINOMETHANE KUNING TELUR TERHADAP KUALITAS SEMEN KAMBING BOER SELAMA SIMPAN DINGIN

TERNAK TROPIKA Journal of Tropical Animal Production Vol 18, No 1 (2017): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Abstract

The objective of this research was to examine the effect of removal raffinose in tris aminomethane egg yolk diluents to semen quality of Boer goat during chilled preservation. This research was carried out at Animal Reproduction Laboratory and Sumber Sekar Laboratory, Animal Husbandry Faculty, Brawijaya University from December 11th 2016 to  January 31th 2017. Semen diluent was divided into two groups, there were P0 (80% Tris Aminomethane + 20% Egg Yolk); and P1 (80% Tris Aminomethane (without raffinose) + 20% Egg Yolk). Parameter of this research was motility percentage, viability percentage, and abnormality percentage. Data of this research was analyzed using paired design t test. The result showed that after five days chilled preservation, was different (P<0,05) on the average motility percentage between P0 (39,50±9,26%) and P1 (43,50±6,26%). There was significantly different (P<0,01) on the average viability percentage between P0 (45,86±15,66%) and P1 (46,38±15,32%). There was significantly different (P<0,01) on the average abnormality percentage between P0 (1,76±0,60%) and P1 (2,31±0,75%). Total motile sperm count after 5 days chilled preservation was different (P<0,05) in P0 treatment and not different (P>0,05) in P1 treatment compared to the expectation value of 40 million sperm/ml. The conclution of this research was the tris aminomethane egg yolk diluent without raffinose could protect of Boer goat semen quality during chilled preservation. Keywords: boer goat semen, tris aminomethane, raffinose, chilled preservation

Pengaruh pengencer air kelapa tua yang berbeda varietas terhadap kualitas semen cair Kambing Boer pada penyimpanan 3-50C

Jurnal Ilmu-Ilmu Peternakan Vol 28, No 2 (2018): Agustus
Publisher : Faculty of Animal Science, University of Brawijaya

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Abstract

Artificial Insemination using liquid semen is technology reproduction can improve goat productivity without liquid nitrogen. The Purpose of this research was to determine differences of old coconut water varieties of red (Cocos rubescens) and green (Cocos viridis) as diluent Boer goat semen, based on motility, viability and spermabnormalities during storage at 3-50C. The materials used for this research was Boer goat fresh semen as much as 3 heads were collected 2 times a week. Experimental Design. The treatment were devided into three Treatmen, there were: P0 (Tris+ 20% egg yolk); P1 (old coconut water varieties red + 20% egg yolk); P2 (old coconut water varieties green + 20% egg yolk). The data were analyzed by randomized block design, if the result are different or significantly different then continued to be tested with Duncans multiple range test. The result showed that during storage until days 4, P0 has the best result on motility (40,5 + 1,1 %), viability (45,18+ 1,56 %), and abnormality (1,89+ 0,59 %). While on the diluent using old coconut water green variety better then red variety. On day of 2 P1 and P2 have motility (61 + 1,3%) and (51+ 7,5%); Viability (67,14+17,99%) and (55,37+10,66%), abnormality (1,23 + 1,02%) dan (1,70+ 0,99%).

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

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Abstract

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety