Articles

Nutrient Intake and Digestibility of Cynomolgus Monkey (Macaca fascicularis) Fed with High Soluble Carbohydrate Diet: A Preliminary Study ASTUTI, DEWI APRI; SUPARTO, IRMA HERAWATI; SAJUTHI, DONDIN; BUDIARSA, I NENGAH
HAYATI Journal of Biosciences Vol 16, No 4 (2009): December 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (29.961 KB) | DOI: 10.4308/hjb.16.4.147

Abstract

High carbohydrate as obese diet is not yet available commercially for monkeys. Therefore, this preliminary study was to carry out nutrient intake and digestibility of cynomolgus monkeys (Macaca fascicularis) fed with high soluble carbohydrate diet compared to monkey chow. Five adult female macaques (average body weight 2.67 kg) were made to consume freshly  diet. Commercial monkey chows (contains 3500 cal/g energy and 35% starch) were fed to three adult females (average body weight 3.62 kg). Nutrient intakes and digestibility parameters were measured using modified metabolic cages. Result showed that  average of protein, fat, starch, and energy intakes in treatment diet were higher than control diet (T-test). Fat intake in the treatment diet was three times higher, while starch and energy intakes were almost two times higher than monkey chow. Digestibility percentage of all nutrients were the same in both diets except for the protein. The study concludes that the freshly prepared high sugar diet was palatable and digestible for the cynomolgus monkeys. Further studies are in progress to develop obese diet high in energy content based on fat and source of starch treatments.         Key words: Macaca fascicularis, obese, metabolic cages, intake, digestibility
Fecal Steroid Profile of Female Javan Gibbons (Hylobates moloch) Maintained in Pairing-Typed Cage Maheshwari, Hera; Sjahfirdi, Luthfiralda; Astuti, Pudji; Purwantara, Bambang; Alikodra, Hadi Sukardi; Sajuthi, Dondin; Widjajakusuma, Reviany
HAYATI Journal of Biosciences Vol 17, No 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.43 KB) | DOI: 10.4308/hjb.17.1.43

Abstract

Estrone conjugate (E1C) and pregnanediol glucuronide (PdG) were predominant steroid metabolites of estrogen and progesterone in feces of most primates and could be used to evaluate ovarian function. These metabolites were determined along with records of genital swelling throughout 3-4 months period from three female Javan Gibbons (Hylobates moloch) maintained in pairing-typed cage at Schmutzer Primate Center, Jakarta (Ullah) and at Taman Margasatwa Taman Sari, Bandung (Donna and Citah). Following methanolic extraction of lyophilized fecal powder, samples were analyzed using enzyme linked immunosorbent assay (ELISA) for E1C and PdG.  In all of the three females observed, both hormone profiles did not indicate any regular cycle of ovarian function even though genital swellings were sometimes observed. In one female (Donna) the hormone patterns showed clear signs of cycle irregularities with extended luteal phase of 40 days and erratic pattern of follicular phase. Of the other two females, no ovarian cycle was found. The data indicate that the fecal steroids analysis is a practical and valuable diagnostic tool for providing reliable information on ovarian function in Javan Gibbon. Factors affected reproductive hormonal profile should be taken in consideration in trying to achieve success in captive breeding program for this species.
IDENTIFIKASI ANTOSIANIN BUAH DUWET (Syzygium cumini) MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI - DIODE ARRAY DETECTION [Identification of Anthocyanins in Jambolan Fruit (Syzygium cumini) by High Performance Liquid Chromatography - Diode Array Detecti Sari, Puspita; Wijaya, Christofora Hanny; Sajuthi, Dondin; Supratman, Unang
Jurnal Teknologi Dan Industri Pangan Vol 20, No 2 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.283 KB) | DOI: 10.6066/4312

Abstract

IDENTIFIKASI ANTOSIANIN BUAH DUWET (Syzygium cumini) MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI - DIODE ARRAY DETECTION [Identification of Anthocyanins in Jambolan Fruit (Syzygium cumini) by High Performance Liquid Chromatography - Diode Array Detection] Puspita Sari1)*, Christofora Hanny Wijaya2), Dondin Sajuthi3), dan Unang Supratman4) 1) Jurusan Teknologi Hasil Pertanian,Fakultas Teknologi Pertanian, Universitas Jember  Kampus Tegalboto Jl. Kalimantan I, Jember 68121 2) Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor Kampus IPB Darmaga, Bogor 16680 3) Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680  4) Jurusan Kimia, FMIPA, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Jatinangor, Sumedang 45363   Diterima  1 Juli  2009 / Disetujui  7 Desember 2009   ABSTRACT   The aim of this study was to determine the content of total monomeric anthocyanins and to identify the anthocyanin composition of jambolan fruit (Syzygium cumini) growing in Indonesia. Anthocyanins were extracted with methanol containing 0.1% HCl, removed from non-polar impurities by partition with chloroform and purified in a C18 solid phase cartridge. Anthocyanins were identified by high-performance liquid chromatography with diode array detection (HPLC-DAD), UV-visible spectral analysis, and physico-chemical reactions. Using pH-differential method, it was known that the content of total monomeric anthocyanin in the ripe fruit was 161 mg/100 g fresh fruit (731 mg/100 g skin on wet basis or 3430 mg/100 g skin on dry basis), based on cyanidin-3-glucoside. Furthermore, five anthocyanins without acylating groups were identified as 3,5-diglucoside derivatives of delphinidin (41.29%), petunidin (27.79%), malvidin (25.60%), cyanidin (4.19%), and peonidin (1.13%). These results demonstrate that the skin of jambolan fruit especially the ripe one is rich in anthocyanin, with wide range in anthocyanidin backbone (five aglycons).   Key words: jambolan fruit, Syzygium cumini, anthocyanin, HPLC-DAD
PRODUCTION OF EMBBRYONIC STEM CELLS FROM INNER CELL MASS OF BLASTOCYST ISOLATED BY ENZYMATIC AND IMMUNOSURGERY METHODS Mata Hine, Thomas; Supriatna, Iman; Sajuthi, Dondin; Boediono, Arief
Jurnal Veteriner Vol 9, No 1 (2008)
Publisher : Jurnal Veteriner

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Abstract

The objective of the research is determining the ICM isolation method to produce ESC. Blastocyst stage of DDy mice embryos were used in this study. Zona pellucida of blastocysts were removed by 0.25% pronase, the ICM isolation were done by enzimatic or immunosurgery method, and then they were cultured in DMEM-high glucose supplemented with mercaptoethanol, gentamycin, fetal bovine serum, and cumulus cells as feeder layer. The result of the research indicated that immunosurgery method yielding attachment rate and number ESC colony 93.85% and 43.08%, respectively, higher (P<0.05) than enzimatic method that weree 79.63% and 18.52%, respectively, but the viability of ICM cells were equal (P >0.05) that are 93.59% in enzymatic method and 98.56% in immunosurgery method. This research concluded that immunosurgery more effective method for isolation of ICM and ESC production than enzymatic method.
DEVELOPMENT OF MICE AND HAMSTER EMBRYOS IN KSOMAA AND HECM-6 MEDIUM Rosadi, Bayu; Agus Setiadi, M; Sajuthi, Dondin; Boediono, Arief
Jurnal Veteriner Vol 9, No 4 (2008)
Publisher : Jurnal Veteriner

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Abstract

The purpose of the present study was to investigate the viability of mice and hamster embryos developed in Kalium Simplex Optimized Medium amino acid (KSOMaa) and Hamster Embryo Culture Medium-6 (HECM-6) medium. Female DDY mice were superovulated by injection i.p. of 5 IU Pregnant Mare Serum Gonadotropine (PMSG) and 5 IU Human Chorionic Gonadotropine (hCG) in 48 h interval, hamster (Phodopus campbelli) injected by 2.5 IU PMSG and 2.5 IU hCG 48 h later. Then females were mated with fertile males. Eight-cell embryos were recovered at day 3 after natural mating. The mice embryos were cultured in KSOMaa+5% NBCS (New Born Calf Serum) (T1) and HECM-6+5% NBCS (T2), the hamster embryos were cultured in KSOMaa+5% NBCS (T3) and HECM-6 + 5% NBCS (T4) for further development at 37oC in a humidified atmosphere of 5% CO2 in air for 48 h. The examinations were replicated five times. The T1 embryos developed to compact morulla and early blastocyst 100% (140/140), 92.1% (129/140) to blastocyst and expanded blastocyst, and 22.9% (32/140) became hatching/hatched. The T3 reached 100% (60/60) to compact morulla and early blastocyst, 85.0% (51/60) blastocyst, and 48.3% (29/60) expanded blastocyst, no embryo observed hatching/hatced. The T2 embryos had more expanded blastocyst than T3 (P<0.05), hatching/hatched rate higher than T1 and T3 but lower than T4 (P<0.05). Shortly, KSOMaa enable to support 8-cell stage mice and hamster embryo, but the hamster embryo developed lower at expanded blastocyst stage. HECM-6 is more appropriate than KSOMaa to support 8-cell mice embryos development and suitable to develop 8-cell stage hamster embryos.
Variasi Molekuler Gen Reseptor Melanokortin-4 pada Monyet Ekor Panjang Arta Putra, I Gusti Agung; Sajuthi, Dondin; Duryadi Solihin, Dedy; Roro Dyah Perwitasari, Raden
Jurnal Veteriner Vol 11, No 3 (2010)
Publisher : Jurnal Veteriner

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Melanocortin-4 receptor (MC4R) is one of G protein-coupled receptors that plays an important role inregulation of energy homeostasis. MC4R mutations constitute the most common cause of human obesity.The study was conducted in order to investigate the variation of MC4R gene in Macaca fascicularis and itsassociation with obesity as a model of human obesity. Forty eight adult male macaques from Bali (Ubudand Uluwatu), East Java (Alas Purwo and Baluran), and Sumatera island (Palembang) were used in thisresearch. The animals had been anaesthetized using ketamine (10 mg/kg body weight) and xylazine (2mg/kg body weight) before collecting blood samples and phenotypic data (weight, crown rump length. Bloodsamples were used as source of DNA. To determine MC4R variation, coding region of this gene wasamplified and sequenced. The results showed that 20 variations sites were identified and 13 of them werenon-synonymous. Among the non-synonymous mutations, five mutations were only found in obese macaque;two mutations were found both in obese and non-obese macaque; and six mutations were only found in nonobesemacaque .
Preservasi Ovarium dan Pengaruhnya Terhadap Morfologi Folikel Domba Rosadi, Bayu; Agus Setiadi, Mohamad; Sajuthi, Dondin; Boediono, Arief
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Jurnal Veteriner

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The purpose of this study was to evaluate the effect of cooling and freezing of ewes ovarian tissue ontheir follicles morphology. The study was carried out in two experiments. Experiment I, ovaries weremaintained in Phosphate Buffered Saline (PBS) at -20oC and room temperature (RT) for 24 h, and at 5oCfor 24 h and 72 h, respectively. After storage, follicles were histologically evaluated. Experiment II, theovarian cortex was isolated and tissue slices (±1 mm3) were prepared. Following this tissues were loadedinto hemistraw then transferred to equilibration solution (PBS+20% FBS+7,5% EG+7,5 % DMSO) atroom temperature and held for 10, 20, 30 minutes, respectively. Afterward tissues were tranferred tovitrification solution (PBS+20%FBS +15%EG+15%DMSO ) for 3 minutes, then the hemistraw was placeddirectly into liquid nitrogen. After thawing, the tissues were prepared for histological examination. All ofthe follicles were deteriorated after 24 h storage at RT. The percentage of morphologically normal follicleswas significantly reduced when ovarian tissues were stored at -20oC for 24 h and at 5oC for 24 h and 72 h.However, it seemed to have a minor deterioration effect when the tissues were kept at 5oC for 24 h(P<0.05). Antral follicles were damaged in all of the treatments. Primordial follicles preserved theirmorphology intactness better than growing follicles. Exposing tissues to equilibration medium for 10minutes seemed to produce higher numbers of morphologically normal follicles (P<0.05), compared towhen tissues were exposed for 20 minutes and 30 minutes (P>0.05). It can be concluded that exposingtissues to equilibration solution for 10 minutes prior to freezing would kept the ovarian follicles morphologyin good condition.
Profil Lipid Darah pada Monyet Ekor Panjang (Macaca fascicularis) yang Diinduksi Diet Tinggi Lemak Suparto, Irma Herawati; Oktarina, Ria; Astuti, Dewi Apri; Mansjoer, Sri Supraptini; Sajuthi, Dondin
Jurnal Primatologi Indonesia Vol 7, No 1 (2010)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

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Abstract

Abstrak: Diet berenergi tinggi dengan sumber dari lemak dapat mendorong perkembangan obesitas yang biasanya disertai dengan perubahan profil lipid darah. Tujuan dari penelitian ini adalah untuk mengevaluasi pengaruh diet energi tinggi dengan sumber lemak hewani dan kuning telur sebagai diet obesitas terhadap profil lipid darah monyet ekor panjang (Macaca fascicularis) yang diberikan selama empat bulan. Lima belas ekor monyet jantan dewasa dibagi menjadi tiga kelompok diet secara acak menjadi kelompok 1) lemak tinggi dari lemak hewani tanpa kuning telur, atau 2) lemak tinggi dengan kuning telur, (lemak 19,62% pada kedua diet), atau 3) diet standar monkey chow sebagai kontrol (lemak 5,55%). Pengukuran dilakukan tiap bulan untuk berat badan, profil lipid darah seperti kolesterol, trigliserida, kolesterol lipoprotein densitas tinggi (HDL-C) dan kolesterol lipoprotein densitas rendah (LDL-C), dan pada akhir studi, konsumsi lemak dan kecernaan dievaluasi. Hasil penelitian menunjukkan hewan konsumsi lemak tinggi dengan kuning telur meningkat bobot badannya secara signifikan pada minggu ke-4 dan 8 (p
Intervensi Nikotin terhadap Level Low Density Lipoprotein, dan Ekspresi UCP-1 (Uncoupling Protein 1) pada Monyet Ekor Panjang (Macaca fascicularis) Obes dengan Resiko Aterogenesis Warongan, Anwar Wardy; Jusuf, Irawan; Sajuthi, Dondin; Sulistiawati, Erni; Mansjoer, Sri Supraptini; Oktarina, Ria
Jurnal Primatologi Indonesia Vol 7, No 1 (2010)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

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Abstract

Abstrak: Lima belas monyet ekor panjang obes (Macaca fascicularis) dengan resiko aterogenesis digunakan untuk memperoleh informasi tingkat low density lipoprotein (LDL) dan ekspresi protein uncoupling-1 (UCP-1) setelah tiga bulan intervensi nikotin dosis rendah per oral. Kelompok hewan dibagi menjadi tiga dengan komponen diet berbeda, yaitu diet A (lemak), diet tipe B (lemak dan kuning telur), dan diet tipe C (komersial diet-monyet chow), dan setiap kelompok terdiri dari lima monyet. Ketiga diet tersebut dicampur nikotin dan diberikan ke dalam makanan sehari dengan dosis 0,50-0,75 mg/kg/12 jam. Pengamatan terhadap lipid darah dilakukan setiap bulan dan pada saat nekropsi diambil jaringan adiposa coklat untuk evaluasi dengan teknik fluoresensi antibodi. Berdasarkan analisis lipid menunjukkan bahwa ada peningkatan yang signifikan konsentrasi LDL (p
Extract of Curcuminoid Temumanangga (Curcuma mangga) in Inhibition Oxidation Reaction of Low Density Lipoprotein by Macrophage Susmiat, Trini i; ., Sulistiyani; Sajuthi, Dondin; Darusman, Latifah K.
Forum Pasca Sarjana Vol 33, No 1 (2010): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

The oxidation of low-density lipoprotein (LDL) is believed to be the initiating factor for the development and progression of atherosclerosis.  Curcuminoid, the metabolite of Zingiberaceae family such as temu mangga (Curcuma mangga), has been shown to reduce the susceptibility of LDL to oxidation. In this study, we examined the effect of curcuminoid extracted from temu mangga on copper ion-induced lipid peroxidation of low density lipoprotein (LDL) in mice’s macrophages and Macaca nemestrina’s monocytes.  Analyses were done by measuring the formation of thiobarbituric acid reactive substance (TBARS) as malonaldehyde (MDA).  LDL were harvested and isolated from 5 adult female Macaca fascicularis  fed aterogenic diet for 3 months.  LDL oxidation by mice macrophage incubated for 4 hours were inhibited by curcuminoid at concentration of 8 ppm.  There was decreased 17% (P
Co-Authors . Tejasari Adi Winarto Afriantini, Iis Agus Wijaya Anita Esfandiari ANTONIUS SUWANTO Anwar Wardy Warongan ARIEF BOEDIONO Bambang Purwantara BAYU FEBRAM PRASETYO Bayu Rosadi C. Hany Wijaya CHAIRIN N MAROEF CHRISTOFORA HANNY WIJAYA Chusnul Choliq Cline, J. Mark Dadang Makmun Daldiyono Daldiyono Darusman, Huda Shalahudin Dedi Rahmat Setiadi, Dedi Rahmat DEDY DURYADI SOLIHIN Dedy Duryadi Solihin DEWI APRI ASTUTI Dewi, Fitriya Nur Annisa DIAH ISKANDRIATI Douglas M. Bowden Dyah Perwitasari-Farajallah Ekowati Handharyani Erdiansyah Rahmi ERIC HAYES ERLIN LISTIYANINGSIH Erni Sulistiawati ESTHER ARIFIN FITRIYA NUR ANISA DEWI Fransiska R Zakaria FUNGKEY HOETAMA HADI SUKADI ALIKODRA Hadi Sukardi Alikodra hajrawati, Hajrawati Hanny Wijaya Harry Soehartono Hastuti, Yohana Tri Henny Nuraini HERA MAHESHWARI I Gusti Agung Arta Putra I Gusti Ngurah Sudisma I Ketut Sutama I NENGAH BUDIARSA I Nengah Budiarsa I WAYAN TEGUH WIBAWAN IETJE WIENTARSIH Iman Rusmana IMAN SUPRIATNA IMELDA WINOTO Irawan Jusuf IRMA HERAWATI SUPARTO Irma Isnafia Arief Jansen Manansang Joesoef, Jayusman Arsiyanti Joko Pamungkas JONATHAN STURGIS Juniantito, Vetnizah KATHARINA JESSICA KEVIN RANDALL PORTER Koeswinarning Sigit Krützen, Michael Latifah K. Darusman Lelana, Agus Leni Maylina Liwa, Setyaningsih Rambu LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirji M Agus Setiadi Maxs Urias Ebenhaizar Sanam Mihardi, Arief Purwo Mohamad Agus Setiadi MOSES AFFANDI Mozes R. Tolihere Muchidin Noordin Muhammad Agil Munggaran, Fauzi Abdurrahman Nater, Alexander Nugroho, Setyo Widi Nur Hidayati Nurhidayat . Oriza Savitri Ariantie Paini Sri Widyati PAINI SRI WIDYAWATI PATRICK J BLAIR Peni Harjosworo PENI SUPRAPTI HARDJOSWORO Permanawati, . PERMANAWATI, PERMANAWATI Prabowo, Teguh Ari PUDJI ASTUTI Puji Rianti Puspita Sari Putri, Zulfitra Utami R. Iis Arifiantini RADEN IIS ARIFIANTINI Raden Roro Dyah Perwitasari RATNA TAN Retno Wulansari Reviany Widjajakusuma Ria Oktarina RICHARD F GRANT Rini Widayanti RR. Dyah Perwitasari Rumlaklak, Yanse Yanne Saefullah, Uus SAEPULOH, UUS SELA SEPTIMA MARIYA Setiadi, Dedi R Setyo Widodo SILMI MARIYA Sri Rahmatul Laila Sri Supraptini Mansjoer Srihadi Agungpriyono SUHARYANTO SUHARYANTO Sulistiyani . SULISTIYANI SULISTIYANI SURYA, MARYATI Sus Derthi Widhyari SUSAN SOKA SUSANA WIDJAJA Taher, Achmad Teguh Budipitojo Thomas Mata Hine Thomas Mata Hine Tri Wahyu Pangestiningsih Trini i Susmiat Trini Susmiati Tuti Suryati Tuty L Yusuf TUTY LASWARDI YUSUF Unang Supratman UUS SAEPULOH Widhyai, Sus Derthi Wilkerson, Gregory K. YASMINA PARAMASTRI Yoelinda, Vincentia Trisna Yuli Purwandari K.