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Acid Resistance Test of Probiotic Isolated from Silage Forage Swamp on In Vitro Digestive Tract Sandi, Sofia; Miksusanti, Miksusanti; Sari, Meisji Liana; Sahara, Eli; Supriyadi, Adi; Gofar, Nuni; Asmak, Asmak
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 4, No 1 (2019): February 2019
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

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Abstract

Antibiotics are commonly used in poultry ration, and afterward these drug residues may persist in foods. There is an alternative source of antibiotics in poultry rations, one of which is using probiotics. Probiotics are living microorganisms capable of reaching the gastrointestinal tract and benefiting health, leaving no residue in the body. Probiotics can be originated from bacteria such as lactic acid bacteria (LAB) which produced lactic acid and antimicrobial components. LAB must be able to live in the digestive system with various pH condition. pH resistance testing of LAB was performed by introducing diluted bacteria into several buffer solutions with different pH and a predetermined incubation time, then was grown in MRS agar medium. This study was conducted to determine the resistance of LAB isolates as probiotics from silage forage swamp at different pH distributed in vitro digestant. The design used was a complete randomized design consist of 3 treatments and 3 replications. The treatments were using isolate of Kumpai Tembaga silage (P1), isolate of 50% Kumpai Tembaga silage and 50% Kemon Air silage (P2), and isolate of Kemon Air silage (P3). The results showed that all isolates used were lactic acid bacteria, and the treatment significantly affected the value of LAB resistance test against low pH and high pH. Isolate from Kumpai Tembaga silage (P1) is a lactic acid bacterium with higher ability to survive in pH of in vitro digestive system.
Peningkatan Indeks Warna Kuning Telur dengan Pemberian Tepung Daun Kaliandra (Calliandra calothyrsus) dan Kepala Udang dalam Pakan Itik Sahara, Eli
Jurnal Sain Peternakan Indonesia Vol 5, No 1 (2010)
Publisher : Universitas Bengkulu

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ABSTRAKTelur itik yang berasal dari pemeliharaan intensif banyak yang pucat, sehingga kurang disukai oleh konsumen. Hal ini disebabkan oleh perobahan pola pemeliharaan dari sistem gembala ke sistem terkurung karena pada sistem terkurung pakan yang diberikan adalah campuran konsentrat, menir dan dedak. Sumber pigmen penguning warna kuning telur dapat diperoleh dari hijauan seperti daun katuk, lamtoro, kaliandra dan kangkung serta dari hewani seperti limbah udang. Pada penelitian ini digunakan 30 ekor itik umur ± 6 bulan yang dibagi dalam 3 perlakuan dan 10 ulangan dengan masing-masing ulangan terdiri dari 1 ekor itik. Perlakuan 1 adalah RB 100%, perlakuan 2 adalah RB + K 6% + CU 3% dan perlakuan 3 adalah RB + K 6% + CU 6%. Hasil penelitian menunjukkan bahwa perlakuan R1 (ransum basal + 6% kaliandra + 3% kepala udang) dapat meningkatkan indeks warna kuning telur dengan skor 11, dan perlakuan R2 (ransum basal + 6% kaliandra + 6%kepala udang) dengan skor 10. Tidak terdapat pengaruh perlakuan kaliandra dan kepala udang terhadap konsumsi ransum dan efisiensi ransum, sedangkan untuk produksi telur terdapat variasi antar individu itik untuk masing-masing perlakuan yaitu itik pada perlakuan R0 hanya 40% yang bertelur, itik pada perlakuan R1 60% yang bertelur dan itik pada perlakuan R2 80% yang bertelur. Kalau dilihat untuk masing-masing perlakuan, itik yang berproduksi telur tinggi untuk perlakuan R0 adalah 75%, R1 yang berproduksi tinggi adalah 66.7% danR2 yang berproduksi tinggi hanya 50%.Kata Kunci : Itik, daun kaliandra, kepala udang, indeks warna kuning telur
Peforman Produksi Ayam Pedaging dengan Pemanfaatan Bungkil Biji Kapas sebagai Pengganti Sebagian Bungkil Kedelai dalam Ransum Sahara, Eli; Sandi, Sofia; Muhakka, Muhakka
Jurnal Sain Peternakan Indonesia Vol 6, No 2 (2011)
Publisher : Universitas Bengkulu

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ABSTRAKMeningkatnya harga ransum, mengharuskan kita mencari bahan alternatif lain  yang harganya lebih murah, salah satunya adalah penggunaan bungkil biji kapas (BBK).  Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan BBK sebagai pengganti sebagian bungkil  kedelai terhadap pertumbuhan ayam broiler.  Penelitian ini menggunakan ayam broiler umur dua minggu.  Ransum perlakuan yang digunakan terdiri dari 4 tingkat  penggunaan BBK sebagai  pengganti bungkil kedelai yakni R0 (0%), R1(6%), R2 (12%) dan R3 (18%).  Rancangan yang digunakan adalah RAL (Rancangan Acak Kelompok) yang terdiri dari 4 perlakuan dan 4 ulangan dan setiap  ulangan terdiri dari 6 ekor ayam dengan menggunakan kandang koloni.  Hasil penelitian menunjukkan  bahwa pada berbagai perlakuan penggunaan BBK berpengaruh sangat nyata (P<0,01) terhadap konsumsi ransum, pertambahan bobot badan (PBB) dan konvesi ransum terbaik diperoleh pada perlakuan R2.  Dari hasil penelitian dapat disimpulkan bahwa penggunaan BBK sebagai pengganti bungkil kedelai terbaik diperoleh pada tingkat 12%.Kata Kunci: bungkil biji kapas, bungkil kedelai , ransum, ayam broiler
Pengaruh Pemberian Daun Kaliandra (Calliandra calothyrsus) dan Kepala Udang terhadap Keamanan Organ Dalam Ternak Itik Sahara, Eli
Jurnal Sain Peternakan Indonesia Vol 5, No 2 (2010)
Publisher : Universitas Bengkulu

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ABSTRAKPenelitian ini bertujuan untuk mencari batas aman pemberian kombinasi pemberian tepung daun kaliandra (Calliandra calothyrsus) dan tepung kepala udang dalam ransum itik tanpa memberikan efek negatif pada produktivitas dari ternak tersebut. Penelitian ini menggunakan 30 ekor itik alabio betina dewasa umur 6 bulan yang dibagi dalam 3 perlakuan dan 10 ulangan dengan masing-masing ulangan terdiri dari 1 ekor itik. Perlakuan 1 adalah RB 100%, perlakuan 2 adalah RB + K 6% + CU 3% dan perlakuan 3 adalah RB + K 6% + CU 6%. Hasil penelitian menunjukkan bahwa tidak terdapat pengaruh perlakuan kaliandra dan kepala udang terhadap konsumsi ransum (P>0,05) serta penggunaan kombinasi kaliandra dan kepala udang tidak berpengaruh terhadap persentase bobot ginjal, pankreas dan hati, ovari dan saluran telur (oviduk) dengan dipertegas oleh hasil histopatologinya yang memperlihatkan morfologi jaringan ginjal, pankreas, hati, ovari dan oviduk yang normal. Dosis kombinasi pemberian daun kaliandra dan kepala udang pada perlakuan ini masih dalam batas normal sehingga tidak memperlihatkan efek negatif terhadap ternakKata Kunci : Daun kaliandra, kepala udang, organ dalam, itik.
The Effect of Fermentation Bran and Chitosan in Ration to Percentage of Tegal Duck Digestive Tract Weight Sahara, Eli; Sandi, Sofia; Yossi, Fitra
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 4, No 1 (2019): February 2019
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

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Abstract

Duck productivity is largely determined by the optimization of bodily functions. The food consumed greatly determines the development of digestive organs and internal organs of livestock. Digestive organs that are well developed and function optimally will be very decisive in turning feed into meat and eggs. Chitosan is a crustacean waste product known as animal fiber and is antimicrobial. Whereas fermented bran contains natural fiber rich in nutrients and is hypocholesterol. The aim of the study was to look at the role of chitosan and bran fermented on the weight percentage of digestive organs of ducks. The study used a completely randomized design (CRD) with 4 treatments and 5 replications. Each test consists of 2 ducks as a unit of experiment. The treatment is R0 = 45% corn + 35% concentrate + 20% bran without fermentation, R1 = 45% corn + 35% concentrate + 20% fermented bran, R2 = 45% corn + 35% concentrate + 19.5% fermented bran + 0.5% chitosan and R3 = 45% corn + 35% concentrate + 17.5% fermented + 2.5% chitosan bran. The variable measured is the weight percentage of the digestive organs (gizzard, small intestine and pancreas) in ducks. Data was processed using SAS Windows 16. The results showed that the administration of fermented chitosan and bran gave an average percentage of gizzard weight (ventriculus) and pancreatic weight percentage was not significantly different between treatments (P> 0.05), while treatment R1 (20% fermented bran) It was shown that the percentage of small intestine weight was higher (31.85%) than control R0.
The Effect of Chitosan Addition to the Digestibility of Dried Matter, Organic Matter and Crude Protein of Tegal’s Duck Rations Sahara, Eli; Widjastuti, Tuti; Balia, Rostita L; Abun, Abun
Indonesian Journal of Fundamental and Applied Chemistry Vol 3, No 2 (2018): June 2018
Publisher : Indonesian Journal of Fundamental and Applied Chemistry

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Abstract

The optimum performance of duck farm can be achieved by providing them with good quality rations. Rations with good digestibility will increase the productivity due to large amount intake of nutrients. Chitosan is type of animal fibre which assisted the growth of useful microbes in digestive system. Addition of chitosan in cattle rations will improve the ecologic of duck digestive system to be more conducive.  The aimed of the research was to evaluate rations digestibility with the addition of chitosan. The research was used completely randomized design with 4 treatments and 5 repetitions. Each repetition consists of 2 ducks. The treatments are R0 stands for rations without chitosan and R1-R3 with addition of chitosan 0.5%, 2% and 2.5% respectively. Parameter measured were dried matter digestibility, organic matter and crude protein. Data processing was conducted by using SAS Windows 16. Result showed chitosan addition at 0.5% and 2.5% gave dried matter digestibility and crude protein does not higher than control (P>0.05) while chitosan addition at 2% gave result lower than control. Organic matter digestibility displays balanced in value.Keywords: Chitosan, Dried matter digestibility, organic matter, crude protein, rations.
Performa Ayam Broiler dengan Penambahan Enzim Fitase dalam Ransum Sahara, Eli; Raudhaty, Erfy; Maharany, Febrika
Jurnal Peternakan Sriwijaya Vol 1, No 1 (2012): Jurnal Peternakan Sriwijaya
Publisher : Program Studi Peternakan Fakultas Pertanian Universitas Sriwijaya

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Abstract

Kecernaan dan daya cerna pakan sangat menentukan dalam menentukan kecukupan nutrien yang diterima tubuh dalam meningkatkan pertumbuhan. Tujuan penelitian ini adalah memicu pertumbuhan ayam broiler dengan penambahan enzim fitase ke dalam pakan. Penelitian ini menggunakan  DOC sebanyak 72 ekor, menggunakan rancangan acak lengkap (RAL) dengan 6 perlakuan dan 3 ulangan, masing-masing ulangan terdiri dari 4 ekor ayam broiler DOC. Terdapat 5 tingkatan dosis enzim fitase dalam perlakuan yaitu; P0= kotrol tanpa pemberian enzim fitase (RB), P1= RB + enzim fitase 500 FTU/kg, P2= RB + enzim fitase + 600 FTU/kg, P3 = RB + enzim fitase 700 FTU/kg, P4 = RB + 800 FTU/kg dan P5 = RB + 900 FTU/kg.  Parameter yang diukur adalah konsumsi ransum, pertambahan bobot badan serta konversi ransum. Hasil penelitian menunjukkan bahwa pemberian enzim fitase sampai level 900 FTU/kg ransum menunjukkan rataan yang hampir sama terhadap konsumsi ransum, pertambahan bobot badan dan konversi ransum (P>0,05).  Namun dari keseluruhan perlakuan pemberian enzim fitase 900 FTU/kg ransum menunjukkan kecenderungan nilai peforma yang lebih baik dengan angka konversi yang paling rendah.Kata kunci : Enzim fitase, ransum, peforma, ayam broile
Peningkatan Asam Lemak Tak Jenuh (Pufas) Dengan Menggunakan Rhizopus Oryzae Dalam Fermentasi Bekatul Sahara, Eli; Yosi, F.; Sandi, Sofia
Jurnal Lahan Suboptimal Vol 5, No 1 (2016): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Sahar et al, 2016. Increasing of Polyunsaturated Fatty Acids (Pufas) by Using Rhizopus Orizae in the  Fermented Bran. JLSO 5(1):79-85.This study aimed to determine 1) the volume of inoculums and the optimum incubation time during the fermentation process; 2) the types of polyunsaturated fatty acids during fermentation; and 3) the presence of omega-3 essential fatty acids in bran fermentation. The study used fermentation method using R. oryzae. Chromatography-Mass Spectroscopy (GC-MS) was used to determine the type of polyunsaturated fatty acids and omega-3 contained in the fermented bran. This study used 9 treatments, V3H3, V3H6, V3H9, V5H3, V5H6, V5H9, V7H3, V7H6, and V7H9. The results showed 1) the volume of inoculums and the fermentation time V7H3 was the most optimum result; 2) there were 13 types of polyunsaturated fatty acids, and 3) there was the content of omega-3 in bran fermented.
The The Change of Nutrients Rations Quality of Feed Fermented with Different Moisture Content Miksusanti, Miksusanti; Sandi, Sofia; Yosi, Fitra; Sahara, Eli; Rofiq, Nasir
Indonesian Journal of Environmental Management and Sustainability Vol 3 No 2 (2019): June
Publisher : ARTS Publishing

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Abstract

ABSTRACT The change of nutrients on rations made from local raw materials was evaluated after being fermented in various moisture contents. The experiment used completely randomized design comprise of 5 treatments and 3 repetitions. The treatments were P0 (fermentation without moisture), P1, P2, P3 and P4 (fermentation in 40%, 50%, 60% and 70% moisture content). Variables observed were total microbe, total acid, acidity (pH) and the change of dried matter content, organic matter, crude protein, crude lipid, crude fiber and Nitrogen-free extract. The result showed that there are statistically significance differences (P<0.05) between local raw materials rations fermented in different moisture contents towards variables observed. Local raw materials rations fermented in 50% moisture content exhibited good nutrients quality indicated by increased in dried matter content, organic matter, crude protein, Nitrogen-free extract, total microbe and total acid while crude lipid, crude fiber and acidity (pH) showed a decreased.