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Prolactin promoter gene as marker assisted selection (MAS) for the control of broodiness of Kampung chicken Sartika, Tike; Mansjoer, S.S; Saefuddin, A; Martojo, H
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 4 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v9i4.433

Abstract

Preliminary research about MAS (Marker Assisted Selection) was conducted to detect broodiness trait of Kampung chicken. MAS currently is very important in situations, where the accuracy of selection is low, such as, traits with low heritability, e.g. broodiness trait and egg production. Prolactin promoter was selected as a marker gene for broodiness because it plays a critical part in the neuroendocrine cascade which is triggered at the onset of broodiness. DNA samples were collected from low and highbroodiness samples on basic population (G0) each 24 samples, and from selected population (G3) each 28 samples. As control population without broody behavior was used 16 samples White Leghorn (WL) chicken. Prolactin promoter gene was amplified using polymerase chain reaction (PCR). PCR product was analyzed using electrophoresis agarose gel 2%. The results showed four types of bands represent in the Kampung chicken, three types called as wild type band and one type as the WL band. The chickens with low and high broodiness on G0 generation have 75 and 87.5% of wild type band while in the G3 generation was decreased to 25 and 75%. Conclusions of the research indicated that the selected breed of the Kampung chicken on G3 generation increased WL band like White Leghorn chicken as much as 31,25% from the G0 generation.   Key words: Kampung chicken, prolactin promoter, MAS
Prolactin promoter gene as marker assisted selection (MAS) for the control of broodiness of Kampung chicken Sartika, Tike; Mansjoer, S.S; Saefuddin, A; Martojo, H
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 4 (2004): DECEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.539 KB) | DOI: 10.14334/jitv.v9i4.433

Abstract

Preliminary research about MAS (Marker Assisted Selection) was conducted to detect broodiness trait of Kampung chicken. MAS currently is very important in situations, where the accuracy of selection is low, such as, traits with low heritability, e.g. broodiness trait and egg production. Prolactin promoter was selected as a marker gene for broodiness because it plays a critical part in the neuroendocrine cascade which is triggered at the onset of broodiness. DNA samples were collected from low and highbroodiness samples on basic population (G0) each 24 samples, and from selected population (G3) each 28 samples. As control population without broody behavior was used 16 samples White Leghorn (WL) chicken. Prolactin promoter gene was amplified using polymerase chain reaction (PCR). PCR product was analyzed using electrophoresis agarose gel 2%. The results showed four types of bands represent in the Kampung chicken, three types called as wild type band and one type as the WL band. The chickens with low and high broodiness on G0 generation have 75 and 87.5% of wild type band while in the G3 generation was decreased to 25 and 75%. Conclusions of the research indicated that the selected breed of the Kampung chicken on G3 generation increased WL band like White Leghorn chicken as much as 31,25% from the G0 generation.   Key words: Kampung chicken, prolactin promoter, MAS
Prolactin promoter gene as marker assisted selection (MAS) for the control of broodiness of Kampung chicken Sartika, Tike; Mansjoer, S.S; Saefuddin, A; Martojo, H
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 4 (2004): DECEMBER 2004
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.539 KB) | DOI: 10.14334/jitv.v9i4.433

Abstract

Preliminary research about MAS (Marker Assisted Selection) was conducted to detect broodiness trait of Kampung chicken. MAS currently is very important in situations, where the accuracy of selection is low, such as, traits with low heritability, e.g. broodiness trait and egg production. Prolactin promoter was selected as a marker gene for broodiness because it plays a critical part in the neuroendocrine cascade which is triggered at the onset of broodiness. DNA samples were collected from low and highbroodiness samples on basic population (G0) each 24 samples, and from selected population (G3) each 28 samples. As control population without broody behavior was used 16 samples White Leghorn (WL) chicken. Prolactin promoter gene was amplified using polymerase chain reaction (PCR). PCR product was analyzed using electrophoresis agarose gel 2%. The results showed four types of bands represent in the Kampung chicken, three types called as wild type band and one type as the WL band. The chickens with low and high broodiness on G0 generation have 75 and 87.5% of wild type band while in the G3 generation was decreased to 25 and 75%. Conclusions of the research indicated that the selected breed of the Kampung chicken on G3 generation increased WL band like White Leghorn chicken as much as 31,25% from the G0 generation.   Key words: Kampung chicken, prolactin promoter, MAS
Desain Sistem Pakar untuk Kontrol Kualitas Pakan Trisyulianti, E; Hardjomidjojo, H; Arkeman, Y; Saefuddin, A
Media Peternakan Vol 28, No 3 (2005): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The objective of feed production process is to generate high quality of feed with parameters, such as good physical, nutrition characteristics, continuous, palatability and safety. It is needed to support the optimum performance of production process. The general objective of this research was to study quality control system and application of quality management in feed production industry. The objective in this research was to design an expert system for feed quality control based on basic knowledge through knowledge acquisition. Model was designed through two steps, i.e. (1) preparation, and (2) development. System development consisted of (a) basic knowledge development in knowledge acquisition, knowledge conceptualization, and knowledge representation, (b) development of inference mechanism, (c) coding, and (d) verification. The Expert System showed that if data out of control, then process was on uncontrollable condition. The Expert System would interpret the causes and recommend the solution that should be done by supervisors/operators. Key words : expert system, feed, quality, process
Y Chromosome Microsatellites Variation in Bali Cattle (Bos sondaicus) Population Winaya, A; Muladno, Muladno; Gurnadi, RE; Saefuddin, A
ANIMAL PRODUCTION Vol 11, No 3 (2009): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Seven Y chromosome specific microsatellites were typed in a sample of 36 unrelated males from Bali cattle breed. Analysis variation of microsatellites marker in Bali cattle were determinate from PCR products by using 7 primer pairs that flanking microsatellites (INRA008, INRA057, INRA062, INRA124, INRA126, DYS 199, and INRA 189).  PCR products were separated by 10% polyacrylamide gel electrophoresis (PAGE), and silver staining method was used to detect allele polymorphism at each locus.  From two different geographical breed origin showed that Bali cattle from Bali island has higher heterozygous (h=0.33) level than Lombok island (h=0.30), but the number of allele was few (only two alleles) in all of locus. It is indicated that Bali cattle from two geographical origins were not significant in genetic variation. We know that Y chromosomal microsatellite in general has tend to specific allele in breed comparing to autosomal chromosome, because allele come from only male or Y sex chromosome and it contrary to autosomal chromosome where allele is contributed from male and female. FAO has specified that minimum four distinct alleles per locus for proficient judgment of genetic differences between breeds and this study we only used one breed, so it may not significantly to discriminate in Bali cattle population.  For next study we need more Y chromosomal microsatellite marker to discriminate more Indonesian breeds related to tracing the genetic potential and because males animal has roles in genetic spreading which can have an enormous impact on highly selected domestic animal populations. (Animal Production 11(3): 149-154 (2009) Key Words : Bali cattle , Y chromosome, microsatellite, allele
Analisis interaksi genotipe-lingkungan pada beberapa sifat kuantitatif ulat sutra (Bombyx mori L) Jakaria, Jakaria; Mansjoer, S S; Saefuddin, A; Kaomini, M
Media Peternakan Vol 24, No 2 (2001): Media Peternakan (Supplement Edition: Animal Production)
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Analisis interaksi genotipe-lingkungan pada beberapa sifat kuantitatif ulat sutra (Bombyx mori L)