Nurma Sabila
Faculty of Pharmacy, Universitas Gadjah Mada

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Aktivitas Antiproliferasi Ekstrak Etanolik Herba Ciplukan (Physalis angulata L.) Terhadap Sel Hepar Tikus Betina Galur Sprague Dawley Terinduksi 7,12-Dimetilbenz[a]antrasena Fauzi, Ilham Agusta; Amalia, Fikri; Sabila, Nurma; Hermawan, Adam; Ikawati, Muthi; Meiyanto, Edy
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
Publisher : Majalah Kesehatan Pharmamedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

One of the natural materials as potentially efficacious chemopreventive agents are Ciplukan (Physalis angulata L.). Several previous studies reported that Physalis angulata L. herbs ethanolic extract (PEE) has cytotoxic activity and induction of apoptosis in breast cancer cells MCF-7 and HeLa cervical cancer cells. This study aims to determine the effects of  PEE as an chemopreventive agent on rat liver cells induced 7,12-dimetilbenz[a]anthracene (DMBA). This study used Sprague Dawley strain female rats aged 40-50 days were divided into 5 groups : (1) DMBA control group, mice were induced with DMBA in per oral dose of 20 mg/kg; (2) DMBA + PEE dose 750 mg/kgBW group ; (3) DMBA + PEE dose 1500 mg/kgBW group ; (4) solvent control group of CMC-Na 0,5%; (5) PEE dose 1500 mg/kgBW control group. PEE was dissolved in CMC-Na 0,5% and administered daily, starting the seventh week after administration of DMBA. At the beginning of  the tenth week of the study, rats were necropted and liver organs were isolated and stored in buffered formalin 4%. Qualitative analysis to determine the histopathology of liver cells through staining method of Hematoxyllin & Eosin (HE), while quantitative analysis to determine the level of liver cell proliferation by AgNOR staining method. The results showed in the DMBA control group that liver cell morphology changes that hiperproliferation leading to carcinogenesis. In DMBA + PEE dose of  1500 mg/kgBW group improved the situation of DMBA-induced liver cells histopathology and antiproliferation activity better than DMBA + PEE dose of 750 mg/kgBW on DMBA-induced rat liver cells. The results showed that Physalis angulata L. herbs ethanolic extract can inhibit cell proliferation in rat liver caused by DMBA administration through antiproliferation mechanism and have potential for the development as chemoprevention material on liver cancer
KULTUR TUNAS Scoparia dulcis, lindernia anagalis, Lindernia ciliata DAN UPAYA BIOREMEDIASI TERHADAP LOGAM BERAT PB, CR, CD Santosa, Djoko; Sabila, Nurma; Dewi, Irma Puspita
Majalah Obat Tradisional Vol 18, No 1 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/mot-TradMedJ18iss1pp%p

Abstract

Telah dilakukan penelitian tentang kultur tunas Scoparia dulcis, Lindernia anagalis, Lindernia ciliata untuk mengetahui kemampuan penyerapan logam berat Pb, Cr dan Cd sebagai proses bioremediasi terhadap polusi logam berat.Tunas-tunas tersebut ditumbuhkan pada media Murashige-Skoog (MS) padat yang ditambah kinetin 2 ppm. Subkultur dilakukan terhadap semua jenis tunas pada umur 21 hari  kemudian dikultivasi pada media MS padat ditambah logam berat 0, 2 dan 5 ppm selama 30 hari. Semua tunas mampu menyerap Cr tetapi tidak mampu menyerap Cd, tunas Lindernia anagalis dan Lindernia ciliata mampu menyerap Pb.
KULTUR TUNAS Scoparia dulcis, lindernia anagalis, Lindernia ciliata DAN UPAYA BIOREMEDIASI TERHADAP LOGAM BERAT PB, CR, CD Santosa, Djoko; Sabila, Nurma; Dewi, Irma Puspita; Aini, Luthfia Nurul
Majalah Obat Tradisional Vol 18, No 1 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tradmedj.7946

Abstract

Telah dilakukan penelitian tentang kultur tunas Scoparia dulcis, Lindernia anagalis, Lindernia ciliata untuk mengetahui kemampuan penyerapan logam berat Pb, Cr dan Cd sebagai proses bioremediasi terhadap polusi logam berat.Tunas-tunas tersebut ditumbuhkan pada media Murashige-Skoog (MS) padat yang ditambah kinetin 2 ppm. Subkultur dilakukan terhadap semua jenis tunas pada umur 21 hari  kemudian dikultivasi pada media MS padat ditambah logam berat 0, 2 dan 5 ppm selama 30 hari. Semua tunas mampu menyerap Cr tetapi tidak mampu menyerap Cd, tunas Lindernia anagalis dan Lindernia ciliata mampu menyerap Pb.
Aktivitas Antiproliferasi Ekstrak Etanolik Herba Ciplukan (Physalis angulata L.) Terhadap Sel Hepar Tikus Betina Galur Sprague Dawley Terinduksi 7,12-Dimetilbenz[a]antrasena Agusta Fauzi, Ilham; Amalia, Fikri; Sabila, Nurma; Hermawan, Adam; Ikawati, Muthi; Meiyanto, Edy
Majalah Kesehatan Pharmamedika Vol 3, No 1 (2011): JANUARI - JUNI 2011
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.133 KB) | DOI: 10.33476/mkp.v3i1.434

Abstract

One of the natural materials as potentially efficacious chemopreventive agents are Ciplukan (Physalis angulata L.). Several previous studies reported that Physalis angulata L. herbs ethanolic extract (PEE) has cytotoxic activity and induction of apoptosis in breast cancer cells MCF-7 and HeLa cervical cancer cells. This study aims to determine the effects of  PEE as an chemopreventive agent on rat liver cells induced 7,12-dimetilbenz[a]anthracene (DMBA). This study used Sprague Dawley strain female rats aged 40-50 days were divided into 5 groups : (1) DMBA control group, mice were induced with DMBA in per oral dose of 20 mg/kg; (2) DMBA + PEE dose 750 mg/kgBW group ; (3) DMBA + PEE dose 1500 mg/kgBW group ; (4) solvent control group of CMC-Na 0,5%; (5) PEE dose 1500 mg/kgBW control group. PEE was dissolved in CMC-Na 0,5% and administered daily, starting the seventh week after administration of DMBA. At the beginning of  the tenth week of the study, rats were necropted and liver organs were isolated and stored in buffered formalin 4%. Qualitative analysis to determine the histopathology of liver cells through staining method of Hematoxyllin & Eosin (HE), while quantitative analysis to determine the level of liver cell proliferation by AgNOR staining method. The results showed in the DMBA control group that liver cell morphology changes that hiperproliferation leading to carcinogenesis. In DMBA + PEE dose of  1500 mg/kgBW group improved the situation of DMBA-induced liver cells histopathology and antiproliferation activity better than DMBA + PEE dose of 750 mg/kgBW on DMBA-induced rat liver cells. The results showed that Physalis angulata L. herbs ethanolic extract can inhibit cell proliferation in rat liver caused by DMBA administration through antiproliferation mechanism and have potential for the development as chemoprevention material on liver cancer