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Uji Fisik dan Kimiawi Pakan Buatan untuk Udang Windu Penaeus

TORANI Vol 19, No 2 (2009)
Publisher : Fakultas Ilmu Kelautan dan Perikanan UNHAS

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Abstract

Using binder in producing artificial feed is significanly needs for water stability of feed. One of the feed binder using in producing shrimp feed is seaweed. The aims of the research is to evaluate the species of seaweed that has better effect as material for shrimp feed binder according to physical and chemical characteristic. The research design used Complete Randomized Design with four treatments and three replicates. The treatments consist of Eucheuma denticulatum, Kappaphycus alvarezii powder and commercial feed as control. Physical parameter of the feed was examined physical, include water stability of feed, hardness, homogenity, the droughning speed, atractant, and feed appetite. The chemical analizys of the feed including examine protein, lipid, and procsimate analysis were done. The result of physical and chemical analysis to the feed in term of variaty of seaweed species shown that the binder from seaweed has better result than artificial feed comercial shrimp. The species of seaweed, G. gigas is better result as binder in shrimp feed. The prosimate analisys has shown the nutrition contain in all feed has been examine, it still in range of tiger prawn.Keywords : Artificial feed, tiger prawn, binder, seaweed, physical dan chemical examine

Quality of tiger shrimp artificial feed using various dosages of seaweed (Gracilaria gigas) meal as binder

Jurnal Akuakultur Indonesia Vol 10, No 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

The quality of an artificial feed particularly the stability of the feed in the water is highly determined by binding agents. The objective of this study was to determine the optimum dosage of seaweed, Gracilaria gigas meal as binder.  Completely randomized design was used in this study, with three dosages of sea weed meal as treatment feeds, i.e. 3, 6, and 9%, with three replications. The control feed was a commercial feed. The physical parameters measured were water stability, hardness level, homogeneity level, sinking rate, attractiveness, and palatability of the feed, and the chemical parameters were protein and lipid dispersion. Based on parameters of hardness level, homogeneity level, protein and lipid dispersion, feed used G. gigas meal of 9% was better than the commercial feed; parameters of sinking rate, attractiveness, and palatability with G. gigas of 9% was equal to the commercial feed; and based on parameters of water stability, the commercial feed was better than treatment feeds. However, when the three treatment feeds were compared, the 9% dosage of G. gigas meal was better than the other two dosages. Based on the results, the feed using seaweed, G. gigas meal of 9% was the best feed. Key words:  Artificial feed, binder, feed quality, G. gigas meal   ABSTRAK Bahan perekat sangat menentukan kualitas pakan buatan, terutama stabilitas dalam air.  Tujuan penelitian ini adalah untuk menentukan dosis tepung rumput laut, Gracilaria gigas yang terbaik sebagai bahan perekat. Penelitian didesain menggunakan rancangan acak lengkap, dengan tiga dosis tepung G.gigas, yaitu 3, 6, dan 9% yang diulang tiga kali.  Pakan komersial digunakan sebagai pakan kontrol.  Parameter fisik yang diukur adalah water stability (kecepatan pecah dan dispersi padatan), tingkat kekerasan, tingkat homogenitas, kecepatan tenggelam, daya pikat dan daya lezat pelet, sedangkan parameter kimiawi adalah dispersi protein dan lemak.  Berdasarkan parameter tingkat kekerasan, tingkat homogenitas, dispersi potein dan dispersi lemak pakan yang menggunakan binder tepung G. gigas 9% lebih baik dibanding dengan pakan komersial; parameter kecepatan tenggelam, daya pikat dan daya lezat pakan yang mengandung binder G. gigas 9% sama dengan pakan komersial; dan berdasarkan water stability, pakan komersial lebih baik dibanding dengan pakan yang menggunakan binder tepung rumput laut G. gigas. Namun bila ketiga pakan perlakuan dibandingkan, pakan dengan binder G. gigas 9% masih lebih baik dibanding dengan kedua pakan perlakuan lainnya.  Sesuai hasil penelitian ini, pakan yang menggunakan binder tepung rumput laut, G. gigas dengan dosis 9% adalah pakan terbaik. Kata kunci:  kualitas pakan, binder, tepung G. gigas, pakan buatan

Effectiveness of Addition Papain Enzyme in Artificial Diet on the Metamorphosis Rate and Glycogen Content of Mangrove Crab Larvae (Scylla olivacea)

TORANI: Journal of Fisheries and Marine Science Vol 1, No 2 (2018)
Publisher : Marine Science and Fisheries Faculty, Hasanuddin University

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Abstract

In the rearing of fish larvae, artificial feed can be given when digestive enzymes have been produced. To improve the ability of fish larvae to utilize artificial feeds can be done with the addition of exogenous enzyme into the artificial feeds. The aim of this study was to determine the dose of papain enzyme and the correct predigest artificial feeding stadium on metamorphosis rate and glycogen content of mangrove larvae (Scylla olivacea). The experimental design used was a factorial pattern with a complete randomized baseline design, the first factor being the dose of papain enzyme (0%, 1.5%, 3.0% and 4.5%), while the second factor was the predigest artificial feeding stage (zoea 2 and 3).  The results showed that the difference of dose of papain enzyme and predigest artificial feeding stages had significant effect (p <0.05) on the metamorphosis rate of  7, 9, 10, 12, 13, 16 and 17 day larvae, but the interaction between them was not significant (p>0,05). The rate of metamorphosis at doses of enzyme papain was 0% and 1.5% longer and significantly different (p <0.05) compared to 3% and 4.5% doses. The rate of metamorphosis at doses of 3.0% and 4.5% was not significantly different (p> 0.05). The content of glycogen larvae in dose of enzyme papain 4,5% not different than dose 1,5% and 3,0% but higher and significantly different than dose 0%. Glycogen content at dose 0%, 1,5% and 3,0%. was not significantly different (p> 0.05). Based on the results of these studies to hydrolyze feed proteins can be used papain enzyme with a dose of 4.5%.Keywords: papain enzyme, metamorphosis rate, mangrove crab larvae (Scylla olivacea), glycogen content

Effect of Predigested Artificial Diet Using Papain Enzyme on the Degree of Protein Hydrolysis and Protease Enzyme Activity of Mud Crab (Scylla olivacea) Larvae at Zoea 2 and 3 Stages

Aquacultura Indonesiana Vol 19, No 2 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

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Abstract

The ability of mud crab (Scylla olivacea) larvae to digest artificial diet depends on the availability of digestive enzyme. To enhance the larvaes capability to utilize artificial diet can be conducted by adding exogenous enzyme to the diet.The aim of this research was to determine the dose of papain enzyme and stadia of predigest artificial diet that produced the best degree of protein hydrolysis and protease enzyme activity. Completely randomized designwas used to evaluate the effect of papain enzyme dose on the degree of protein, with four treatment and three replications, namely the doses of 0.0%, 1.5%, 3.0%, and 4.5%. Factorial pattern with the completely randomized design was used to evaluate the effect of papain enzyme dose and the proper predigest artificial feeding stage on the activity of protease enzyme. The first factor was the papain enzyme dose (0.0%, 1.5%, 3.0%, and 4.5%), while second factor was the stadia of larvae when it is fed with predigested artificial diet (zoea 2 and zoea 3 stadia). The research showed that the difference of papain enzyme dose provides real effect on degree of protein hydrolysis either on JP 0 or JP 1 diet brands (P<0.05). Degree of protein hydrolysis of the diet predigested with 0.0% papain enzyme was the lowest and has real difference (P<0.05) with the 1.5%, 3.0% and 4.5% doses. Degree of protein hydrolysis on 3% dose of papain enzyme does not provide real difference with the 4.5%, namely  19.753% and 22.890% respectively in JP 0, 18.707 % and 20.430 % in JP 1, but has real difference (P<0.05) with 1.5% doses.  The difference papain enzyme dose and  stadia of predigest artificial diet as well as the interaction between both of them had significant effect  (p<0.05) on the activity of protease enzyme. On the larvae of zoea 2 stadia, the activity of protease enzyme on the larvae fed with predigest artificial diet starting from zoea 2 stadia with 3.0% and 4.5% papain enzyme dose or those still fed with natural diet does not provide real difference, but is higher and provides real difference than 0% and 1.5% doses. On the larvae of zoea 3 stadia, the activity of protease enzyme to the larvae fed with artificial diet starting from zoea 2 with 0% and 1.5% papain enzyme doses, and those fed with artificial diet starting from zoea 3 stadia with 0% papain enzyme dose does not provide real difference (p>0.05) but is lower and provides real difference than other treatments. Protease enzyme activity on the larvae  fed with predigest artificial diet using  4.5%  papain enzyme  starting at zoea 2 stadia ,  was not significantly different (P <0.05) compared to larvae fed artificial diet which was  predigest  with 0.0%,  1.5%, 3.0 and 4.5  papain enzyme started at Zoea 3.  Based on the degree of protein hydrolysis, papain enzymes can be used to hydrolyze artificial diet  in doses ranging from 3.0% to 4.5%. Based on the activity of protease enzymes, mud crab larvae (Scylla olivacea) predigested artificial diet using 4.5% papain enzyme can be provided to the larvae starting from zoea 2 stadia.