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A TYPE OF DWARFISM FOUND IN SOUTH BENGKULU TO BE AN X-LINKAGE MUSLIM, CHOIRUL; KARYADI, BHAKTI; RUYANI, ACENG; PURNAMAWATI, ICE; -, HERNANI; -, SUMIYATI
Jurnal Biologi Vol XII, No 2
Publisher : Jurnal Biologi

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We have reported here a specific human short stature population in Kedurang District, South Bengkulu. AH those are men, described as a mild dwarf (130-150 cm), detected after 5 to 10 years old, caused by the failure on lumbar backbone growth and development, and only found among the men. In this investigation, we examine the inheritance pattern of the dwarfism by using pedigree analysis, study the morphological comparison between the disorder and normal person, and finally we ascertain the variation among the disorder. We accomplished a survey on pedigree family to at least 3-4 generations related to the disorder. The data are then tested with Chi-square test, and transformed to tree families and pedigree analysis. To investigate morphological performance and their variation, we collected morphometric measurements to both disorder and normal group of sample; they are the height, the vertebral bones length, head measurement, the leg and arm length (upper and fore side), and their indexes. The data were analyzed descriptively. To find out the variation, the quantitative expression and penetrance, we evaluated the data according to normal distribution with Z test. The result showed all the dwarfisms in Kedurang are men, who inherited his gene from his carrier mother. As a result, the gene has tightly linked to X chromosome and supposedly belongs to Spondylo Epiphypeseal Dysplasia Tarda (SEDT). These were concluded from 32 related family of dwarfism. We found 19 dwarf persons, grouped into young, adult, and older persons. The their height were shorter than normal (136,5 cm), and the length of vertebrae was under normal value (51,5 cm). Their frontal extremity were shorter than the normal one, but they are in proportion with any part comprised them. Their feet were shorter, but the proportion of leg is longer than calf. The rest is as normally. Among the dwarf person, there are variations of the length. The phenotypic expressivity is varied in the vertebrae, leg, and calf; but the rest is relatively invariable. Their penetrance is strong, mostly in the height, length of vertebrae, and legs. Based on morphological data we concluded that the disorder in the Kedurang, South Bengkulu belongs to SEDT (Spondyloepiphyseal dysplasia tardd).
Development of The Stem of Jatropha multifida Linn as A New Antimalarial through Erythrocytes Test on Mus musculus Infected by Plasmodium berghei Sundaryono, Agus; Ruyani, Aceng; Sari, Rika Partika
Journal of Biomedicine and Translational Research Vol 1, No 1 (2015): April 2015
Publisher : Faculty of Medicine, Diponegoro University

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Abstract

Introduction : Jatropha multifida Linne is known as Betadin plant by the Bengkulunese. The stem extract of J. multifida has antibacterial activity. This study examined the potential of stem extract of J. multifida to be developed as an anti-malarial drugs through trials in M. musculus whose erythrocytes infected by Plasmodium berghei.Methods : Phytochemical test of the stem of J. multifida to test the flavonoids, alkaloids, tannins, saponins, terpenoids and steroids. The stem extract of J. multifida obtained by maceration with 96% ethanol. 30 M. musculus divided 6 groups, each consist of 5 animals. P0 is the negative control groups that were not infected by P berghei. P1 is the positive control groups that were only infected by P berghei, P2 is a comparison groups that were infected with P berghei and chloroquine, groups P3, P4, and P5 were infected by P berghei and treated with stem extract of J multifida with consecutive doses 0.028 g / kgbw, 0.056 g / kgbw and 0.084 g / kgbw. After 24 hours, the number of erythrocytes was observed with hemocytometer then was counted the number of uninfected erythrocytes by P berghei using a microscope at 1000x magnification.Results :The stem extract of J. multifida at doses of 0.028 g/kgbw, 0.056 g/kgbw and a dose of 0.084 g/kgbw were able to increase the number of uninfected erythrocytes by P berghei in M. musculus, respectively an average of 9.135million cell eritrosit/mm3, 7.618 millioncell eritrosit/mm3, and 9.856 millioncell eritrosit/mm3. The ability of stem extract of J. multifida in increasing the number of erythrocytes uninfected with P berghei was much higher than the malaria drug chloroquine diposphat. On one way ANOVA analysis of Fcount ( 13,2 ) > Ftable (2.76 ) , with (α = 0.05), there are noticeable differences in the provision of treatment. The increasing number of uninfected erythrocytes by P berghei was due to the content of flavanol glycosides in J multifida stem. Flavanol glycosides was expected to form Flvanolglycosides-heme complex that could inhibit the formation of P berghei parasites HemazoinConclusion:The stem extract of J. multifida could be developed as a potential anti-malarial drugs since it could increase the number of uninfected erythrocytes by P berghei in M. musculus. Its ability was much higher than the malaria drug chloroquine diposphat
Pengaruh Pemberian Ekstrak Kijing (Pilsbryoconcha exilis) Pada Pemulihan Kualitas Sperma Mencit (Mus musculus) Sari, Erna Yunita; Karyadi, Bhakti; Ruyani, Aceng; Muslim, Choirul
PendIPA Journal of Science Education Vol 3, No 1 (2019): FEBRUARY
Publisher : University of Bengkulu

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ABSTRACT[Effect of Kijing Extract (Pilsbryoconcha exilis) on the Recovery of Sperm Quality in Mice (Mus musculus)]. This study aims to determine the effect of Pilsbryoconcha exilis extract on the quality of M. muscullus sperm. The approach used for the study was experiment with a complete randomized design model. In this study, 4 treatments and 5 replications were conducted, including treatment 1 which only administered aquades as control (P0), treatment 2 which only given paracetamol dose 250 mg / kg (P1), and treatment 3 given paracetamol dose 250 mg / kg and P. exilis extract dose 0,46 mg / g (P2) and treatment 4 given paracetamol 250 mg / kg and extract P. exilis 0,69 mg / g (P3). Technique of data analysis using qualitative descriptive and statistical test of One Way Annova parametry, and if obtained significant difference then continued statistic test of Smallest Real Difference. From the results of the study, it was found that P. exilis and paracetamol extract had no effect on sperm quality. Keywords: Sperm; M. muscullus; extract P. exilis; paracetamol.(Received August 14 , 2018; Accepted January 20, 2019; Published February 26, 2019) ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak Pilsbryoconcha exilis terhadap kualitas sperma M. muscullus yang diberi parasetamol. Pendekatan yang digunakan untuk penelitian adalah eksperimen dengan model rancangan acak lengkap (RAL). Pada penelitian ini menggunakan 4 perlakuan dan 5 ulangan meliputi perlakuan 1 yang hanya diberikan aquades merupakan kontrol (P0), perlakuan 2 yang hanya diberikan parasetamol dosis 250 mg/kg bb (P1), dan perlakuan 3 yang diberikan parasetamol dosis 250 mg/kg bb dan ekstrak P. exilis dosisi 0,46 mg/g bb (P2) dan perlakuan 4 yang diberikan parasetamol 250 mg/kg bb dan ekstrak P. exilis 0,69 mg/g bb (P3). Teknik analisa data menggunakan deskriptif kualitatif dan uji statistik parametri One Way Annova, dan jika didapatkan perbedaan yang bermakna maka dilanjutkan uji statistic Beda Nyata Terkecil (BNT). Dari hasil penelitian diketahui pemberian ekstrak P. exilis dan parasetamol berepengaruh tapi tidak signifikan terhadap kualitas sperma. Kata Kunci: Sperma; M. muscullus; ekstrak P. exilis; parasetamol.
The Capability of Total Protein Extracted from Black Sea Cucumbers (Holothuria leucospilota) in Agglutinating of Mouse Sperms (Mus musculus) Haryanto, Hery; Ruyani, Aceng; Piramida, Cally
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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The research aimed to know the effect of total protein extracted from black sea cucumbers (Holothuria leucospilota) on agglutination of mouse sperms (Mus musculus). The flesh of black sea cucumber  was homogenized in Tris Buffer Saline (TBS) at pH 7.4. The homogenate was added with ammonium sulphate 50%(w/v) for presipitating protein. The presipated protein was purified with 12.000 MWCO dialysis bag to remove ammonium sulphate. The purified protein was quantitated with Biuret Method. The concentration of purified protein was 4,97g/dL. There were five concentration proteins used mouse sperm agglutination test, i.e. 4.97, 3.52, 2.48, 1.17 g/dL and 0% (control). Mouse sperm suspension was prepared by soaking an epypydimal duct in 0,9% (w/v) NaCl solution. The purified protein in varied concentration was then used for agglutination of suspension of mouse sperm. This research used Completely Randomized Design (CRD) with 5 concentrations of protein  and 5 replications. The data of this research were analyzed by using ANOVA. The result showed that total protein extract of black sea cucumberwas significantly different on mouse sperm agglutination. The fastest time of agglutination of was indicated by concentrations of  4.97 g/dL. total proten of black sea cucumber. Keywords: Black Sea Cucumber (Holothuria leucospilota); mouse sperm suspension; sperm agglutination; total protein extract
Respons mahasiswa terhadap buku panduan dan kegiatan pelatihan “teknik monitoring kura-kura cyclemys oldhamii” Puji Astuti, Annisa; Ruyani, Aceng; Wiryono, Wiryono
PendIPA Journal of Science Education Vol 1, No 1 (2017): october
Publisher : University of Bengkulu

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Protein yang Terkait dengan Teratogenisitas Anggota Tubuh Mencit Swiss Webster Akibat Perlakuan dengan Asam Metoksiasetat (MAA) Ruyani, Aceng; Sudarwati, Sri; A . Sutasurya, Lien; H. Sumarsono, Sony
Journal of Mathematical and Fundamental Sciences Vol 33, No 3 (2001)
Publisher : ITB Journal Publisher, LPPM ITB

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Abstract

Telah diteliti protein yang terkait dengan teratogenesis anggota tubuh mencit Swiss Webster akibat perlakuan dengan MAA. Mencit umur kebuntingan 11 hari diberi perlakuan dosis tunggal MAA 10 mmol/kg berat badan secara gavage, sedangkan kelompok kontrol hanya diberi pelarut akuabides steril. Mencit bunting dibunuh secara dislokasi leher 4 jam setelah perlakuan dengan MAA. Tunas anggota tubuh depan diisolasi dari kelompok kontrol dan perlakuan lalu dihomogenisasi. Ekstrak kasar kemudian difraksinasi dengan amonium sulfat dan masing-masing fraksi dianalisis dengan teknik l-D dan 2-D SDS-PAGE. Elektroforegram l-D dan 2-D menunjukkan bahwa pada kelompok perlakuan fraksi protein ammonium sulfat 20-40 % (F-lI), dapat dideteksi protein 31,0-36,5 kDa serta bercak protein 35,1 kDa, pI 6,2 yang tidak terdapat pada kontrol. Pada kelompok perlakuan fraksi protein ammonium sulfat 40-60% (F-lll), dapat dideteksi protein 66,3-97,4 kDa serta bercak protein 8 I,7 kDa, pl 7,3 yang tidak terdapat pada kontrol. Sedangkan pada kelompok kontrol F-llI, protein 36,5-55,4 kDa sertab ercakp rotein 41,6 kDa, pI 6,4 terdeteksi, tetapi tidak terdeteksi pada kelompok perlakuan. Dari penelitian ini dapat disimpulkan bahwa pada tunas anggota tubuh depan mencit, perlakuan dengan MAA menginduksi ekspresi dua protein(35,1 kDa, pl 6,2 dan 81,7 kDa, pl 7,3) dan menghambat ekspresi satu protein(41,6 kDa, pl 6,4). Proteins which are Linked with Swiss Webster Mouse Limb Teratogenesis as the Effects of Methoxyacetic Acid (MAA) TreatmentThe analysis of proteins, which are linked with limb teratogenesis as the effects of MAA treated in Swiss Webster mouse has been investigated. A single dose of MAA 10 mmol/kg body weight was given by gavage on gestation day 11 , whereas the control group were administered sterilized distilled water. Pregnant mice were sacrificed by cervical dislocation at 4 hours after MAA treatment. The forelimb buds were isolated from both control and treated group embryos and were then homogenized. The crude extracts were Then fractionated with ammonium sulfate and each fraction was analyzed by 1-D and 2-D SDS-PAGE techniques respectively. The l-D and 2 -D electrophoregrams revealed that in the treated group of protein fraction 20-40% ammonium sulfate ( F-II), a protein of 31.0-36.5 kDa and a protein spot 35.1 kDa, pl 6.2 could be detected, which was not found in the control. In the treated group of protein fraction 40-60% ammonium sulfate( F-lll) a protein of 66.3-97.4 kDa and a protein spot 81.7 kDa, pl 7.3 could be detected which was not found in the control, whereas in the control group a protein of 36.5-55.4 kDa, which is a protein spot4 1.6 and p l 6.4, was detected but not detected in the treated group.It could be concluded from this experiment that in the mouse forelimb buds, MAA treatment induce the protein expression of two proteins(35.1 kDa, pl 6.2 and 81.7 kDa, 7.3) and inhibit the expression of one protein (41.6p, l 6.4).
Beberapa Kejadian Cacat Bawaan Bayi Lahir Di Rumah Sakit M. Yunus Bengkulu Dalam Satu Dekade Terakhir MUSLIM, CHOIRUL; MARNIS, MUSNI; NADYA, NADYA; HARYANTO, HERRY; KARYADI, BHAKTI; RUYANI, ACENG
Prosiding Seminar Biologi Prosiding Seminar Nasional From Basic Science to Comprehensive Education
Publisher : Prosiding Seminar Biologi

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Abstract

Untuk membandingkan jenis dan jumlah kejadian cacat bawaan pada bayi lahir, kami telah melakukan dua kali survey rekam medis, dan observasi langsung di RumahSakit M.Yunus dalam jarak lebih dari sepuluh tahun. Pada tahun 2000 kami mengolah data rekam medis periode Agustus 1998 s/d Juli 1999, dan mengamati setiap kelahiran bayi yang lahir di rumah sakit tersebut secara langsung selama bulan Juli 1999. Pada tahun 2013 kami sekali lagi mengolah yang sama pada periode Januari 2010 s/d Juli 2011, dan mengamati setiap kelahiran bayi selama bulan Juli 2011. Dari survey dan pengamatan langsung tersebut kami mencatat jenis-jenis cacat bawaan bayi lahir di Rumah Sakit M.Yunus Bengkulu, dan membandingkan jumlah dan jenisnya. Hasil penelitian menunjukkan ada 6 jenis cacat bawaan bayi lahir yang cenderung meningkat dari tahun ke tahun, ialah Atresia Ani, Hirschprung, Gastrochisis, Spina bifida, Hernia, dan Hydrocephalus. Peningkatan resiko cacat bawaan bayi lahir tersebut diduga berkaitan dengan semakin banyaknya penggunaan bahan kimia beresiko teratogenik, dan mutagenik di dalam asupan bahan makanan danobat-obatan di luar resep dokter. Kata kunci:   neonatus congenital, rekam medis, Atresia Ani, Hirschprung, Gastrochisis, Spina bifida, Hernia, Hydrocephalus, teratogenik
Uji Aktivitas Ekstrak Biji Caesalpinia Sp terhadap Jumlah Eritrosit Mus musculus yang Diinfeksi Plasmodium berghei serta Implementasinya dalam Pembelajaran Kimia Nurleli, Nurleli; Sundaryono, Agus; Ruyani, Aceng
PendIPA Journal of Science Education Vol 2, No 3 (2018): October
Publisher : FKIP University of Bengkulu

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AbstraCT[Activity test of Caesalpinia sp seed extract on erythrocytes amount of Mus musculus infected by Plasmodium berghei and its implementation in chemistry learning]. The aims of this study were to (1) determine the activity of seed extract (Caesalpinia sp) on the number of Erythrocytes of M. Musculus of Swiss Webster Strain infected by P.berghei, (2) Find  the active compounds obtained from the isolation of Caesalpinia sp seed extract (3) Find the differences in student learning outcomes about the separation of secondary metabolites using thin layer chromatography (TLC) which is taught in chemistry learning by using LKS. In this study Caesalpinia sp seed extract was purified by thin layer chromatography and column coromatography, and was identified by IR and 1H-NMR spectroscopy. M. musculus infected with P. berghei was given Caesalpinia sp seed extract at a dose of 0.028 g/Kgbb, 0.056 g/Kgbb, and 0.084 g / Kgbb by gavage, while the positive control group was given Kloroquin diphosphate, negative control was given olive oil. Caesalpinia sp seed extract with a dose of 0.056 g/Kgbb inhibited the highest plasmodium, which was 42.89% and even exceeded the malaria drug commonly used chloroquin diphosphate 29.85%. Caesalpinia sp seed extract capable of reducing plasmodium in erythrocytes is also able to increase the number of uninfected erythrocytes. The active compounds obtained from the isolation of Caesalpinia sp seed extract are alkaloids, flavonoids, saponins, and terpenoids. In the implementation of extracurricular group learning is taught by using worksheets. There are significant differences where the average posttest score is 88.67 and the average pretest is 32.1. Keywords: Caesalpinia Sp.; Erythrocyte; Plasmodium berghei; Mus muscullus; Student working sheet.
Studi perbandingan adaptasi Kura-Kura Pipi Putih (Siebenrockiella crassicollis) jantan dan betina di area Kolam Konservasi Universitas Bengkulu Pasaribu, Julita; Ruyani, Aceng; Suhartoyo, Hery
PendIPA Journal of Science Education Vol 3, No 1 (2019): FEBRUARY
Publisher : FKIP University of Bengkulu

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ABSTRACT[Adaptation study of Pipi Putih (Siebenrockiella crassicolli) sexes in conservation pond at University of Bengkulu]. This study aims to compare the adaptability of Pipi Putih turtles (Siebenrockiella crassicollis) of males and females in the conservation pond area of University of Bengkulu (UNIB). Nine S.crassicollis were kept individually in a cage, consisting of three space at three different point. S.crassicollis were fed with kangkung (Ipomea aquatica) as much as 10% of  their body weight. Growth parameters was collected every week for six weeks : (a) weight gain (WG); (b) thick growth of body (TGB); (c) growth of carapace length (GCL), (d) growth of carapace width (GCW); (e) growth of plastron length (GPL)  and (f) plastron width growth (PWG)  The measurement results show: (a) WG: male = 3.50%, female = 2.01% (b) TGB: male = 0.30%, female = -0.05%; (c) GCL: male = 0.16, female = 0.26%, (d) GCW : male = 0.566%, female = 0.47% (e) GPL: male = 0.28%, female = 0, 27% and (f) PWG: male = 1.25%, female = 1.16%. Mean turtle growth percentage of all indicators: male S. crassicollis = 0.01% and 0.69% for female S. crassicollis. We concluded that the adaptability of male S. crassicollis turtles was higher than for female S. crassicollis. Keywords: Conservation; Siebenrockiella crassicollis; turtle; adaptation.(Received August 16, 2018; Accepted January 19, 2019; Published February 26, 2019) ABSTRAKPenelitian ini bertujuan untuk membandingkan kemampuan adaptasi kura-kura pipi putih (Siebenrockiella crassicollis) jantan dan betina di area kolam konservasi Universitas Bengkulu (UNIB). Sembilan ekor S.crassicollis dipelihara secara individu di dalam kerambah. Terdapat tiga kerambah yang diletakkan pada tiga titik berbeda. Kangkung (Ipomea aquatica) merupakan pakan yang diberikan sebanyak 10% dari berat badan. Pengambilan data dilakukan sekali seminggu selama enam pekan. Parameter pertumbuhan yang diamati adalah (a) pertumbuhan berat badan (PBB); (b) pertumbuhan tebal badan (PTB); (c) pertumbuhan panjang karapaks (PPK), (d) pertumbuhan lebar karapaks (PLK); (e) pertumbuhan panjang plastron (PPP) dan (f) pertumbuhan lebar plastron (PLP). Hasil pengukuran menunjukkan: (a) PBB: jantan = 3,50 %, betina = 2,01% (b) PTB: jantan =  0,30 %, betina = -0,05% ; (c) PPK : jantan =  0,16, betina = 0,26 %, (d) PLK : jantan = 0,566%, betina = 0,47%  (e) PPP: jantan = 0,28%, betina = 0,27%  dan (f) PLP: jantan = 1,25%, betina = 1,16%. Rata-rata persen pertumbuhan kura-kura dari seluruh indikator: S.crassicollis jantan = 0,01% dan 0,69% untuk S.crassicollis betina. Berdasarkan data yang diperoleh,disimpulkan bahwa tingkat adaptasi kura-kura S.crassicollis jantan lebih tinggi daripada S. crassicollis betina. Kata kunci: Konservasi; Siebenrockiella crassicollis; kura-kura; adaptasi. 
PENGEMBANGAN LKS MELALUI KEGIATAN IOI UNTUK MENILAI KOGNITIF DAN PERILAKU KONSERVASI SISWA SMA Prihatiningsih, Nur’azizah; Ruyani, Aceng; Ansyori, Irwandi
Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi Vol 1, No 1 (2017)
Publisher : Unib press Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (757.238 KB) | DOI: 10.33369/diklabio.1.1.65-71

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Jenis penelitian yang digunakan yaitu penelitian dan pengembangan (RnD). Masalah dalam penelitian ini yaitu: 1) bagaimana mengembangkan LKS konservasi kura-kura sehingga layak digunakan pada materi pembelajaran ekosistem, 2) apakah implementasi LKS hasil pengembangan melalui kegiatan Indoor-Outdoor-Indoor (IOI) dapat meningkatkan kognitif siswa, 3) apakah implementasi LKS hasil pengembangan melalui kegiatan IOI dapat meningkatkan kecenderungan berprilaku konservasi bagi siswa. Penelitian ini bertujuan untuk: 1) menganalisis kelayakan pengembangan LKS konservasi kura-kura pada materi pembelajaran ekosistem, 2) menilai kognitif siswa melalui kegiatan IOI menggunakan LKS konservasi kura-kura Sumatera dan 3) menilai perilaku konservasi siswa melalui kegiatan IOI menggunakan LKS konservasi kura-kura Sumatera. Penelitian ini menggunakan metode kuantitatif kualitatif deskriptif. Pembelajaran dengan menggunakan strategi indoor-outdoor menggunakan LKS konservasi kura-kura Sumatera. Untuk menilai kognitif siswa menggunakan tes tertulis berupa pretest dan posttest, sedangkan untuk menilai perilaku konservasi siswa menggunakan angket prilaku konservasi. Pembelajaran indoor dilaksanakan di sekolah dan pembelajaran outdoor dilaksanakan ditempat konservasi kura-kura Sumatera di Universitas Bengkulu. Uji kelayakan bahan ajar LKS berupa aspek materi dan aspek kebahasaan, dengan persentase 80-88 % memiliki kriteria “sangat layak” untuk di uji cobakan dilapangan. Ketuntasan belajar klasikal (kognitif) dengan persentase 15,3 %, tidak tuntas secara klasikal. Ketidaktuntasan dikarenakan penyampaian konsep materi oleh peneliti dirasa kurang dan peserta didik tidak menyimak pembelajaran dengan baik. Kecenderungan berperilaku konservasi awal siswa dengan persentase 18,43 % dan prilaku konservasi akhir siswa dengan persentase 22,71 %, mengalami peningkatan 4,28 %.Kata kunci: Kura-kura Sumatera, Research and Development (RnD), Lembar Kerja Siswa (LKS), Indoor-Outdoor