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Mia Miranti Rustama
Jurusan Biologi Fakultas MIPA Uiversitas Padjadjaran

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UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK AIR DAN ETANOL BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI GRAM NEGATIF DAN GRAM POSITIF YANG DIISOLASI DARI UDANG DOGOL (Metapenaeus monoceros), UDANG LOBSTER (Panulirus sp), DAN UDANG REBON (Mysis dan Acetes) Lingga, Martha Elselina; Rustama, Mia Miranti
Biotika (Jurnal Ilmiah Biologi) Vol 5, No 2 (2006): Biotika Desember 2006
Publisher : Biotika (Jurnal Ilmiah Biologi)

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The research has been conducted to experiment an activity of antibacterial assay from water and ethanol garlic (Allium sativum L.) extract to Gram negative and Gram positive Bacteria isolated from Metapenaeus monoceros, Panulirus sp, Mysis and Acetes (Prawns). The research used factorial pattern Randomize Block Design which consisted of two factors and two replications. An assay bacteria are Bacillus sp, Streptococcus sp, Staphylococcus sp, Erysilopethrix sp (Gram positive) and Clostridium sp, Corynebacterium sp, Plesiomonas sp, Vibrio sp (Gram negative), have been used. The result showed that all concentrations of garlic ekstract with water and ethanol solvent has been influence the zone of inhibition from all bacteria. The pure garlic ekstract with concentration 75 % showed the best zona of inhibition to Streptococcus sp (28.25 mm), Clostridium sp (27,75 mm), and Plesiomonas sp (22.25 mm).
UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK AIR DAN ETANOL BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI GRAM NEGATIF DAN GRAM POSITIF Rustama, Mia Miranti; Rahayuningsih, Sri Rejeki; Kusmoro, Joko; Safitri, Ratu
Biotika (Jurnal Ilmiah Biologi) Vol 4, No 2 (2005): Biotika Desember 2005
Publisher : Biotika (Jurnal Ilmiah Biologi)

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The research has been conducted to experiment an activity of antibacterial assay from water and ethanol garlic (Allium sativum L.) extract to Gram negative and Gram positive Bacteria. The research used factorial pattern Randomize Block Design which consisted of two factors and two replications. An assay bacteria are Acetobacter aceti, Pseudomonas aeruginosa, and Shigella sp. (Gram negative) and Bacillus subtilis, Streptococcus sp. and Staphylococcus sp. (Gram positive) have been used. The result showed that all concentrations of ekstract  allium with water and ethanol solvent has been influence the zone of inhibition from all bacteria. The pure garlic ekstract with water solvent with concentration 75 % showed the best zona of inhibition to Streptococcus sp. (28.5 mm) and Shigella sp. (11,5 mm).
Pengaruh Suhu Pengeringan Polyhedra Terhadap Efektivitas Helicoverpa Armigera Nuclear Polyhedrosis Virus (HaNPV) Korespondensi: Dr. Mia Miranti, MP. Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Padjadjaran, emal : miamiantariksa@gmail.com. Subkultur dalam Mengendalikan Populasi Spodoptera litura Fabricius Marissa, Putri; Rustama, Mia Miranti; Hermawan, Wawan
Biotika Vol 11, No 1 (2013): BIOTIKA JUNI 2014
Publisher : Biologi Universitas Padjadjaran

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Penelitian mengenai pengaruh suhu pengeringan polyhedra terhadap efektivitas Helicoverpa armigera NuclearPolyhedrosis Virus (HaNPV) subkultur dalam mengendalikan populasi Spodoptera litura Fabricus telah dilakukan.Penelitian dilakukan dengan metode eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) faktortunggal dengan 5 taraf perlakuan (HaNPV cair dan HaNPV yang dikeringkan pada suhu 4oC, 25C)dengan empat kali pengulangan. Hasil penelitian menunjukkan bahwa berat badan maksimum larva S. litura instartiga mencapai 0,967 g terjadi pada larva yang terinfeksi HaNPV yang dikeringkan pada suhu 4C dan pertambahanberat badan mencapai 0,732 g/ekor/hari pada larva yang terinfeksi HaNPV yang dikeringkan pada suhu 4C
EFEKTIVITAS FORMULASI Helicoverpa armigera Nuclear Polyhedrosis Virus SUBKULTUR (HaNPV1 ) DALAM BERBAGAI BAHAN PEMBAWA TERHADAP MORTALITAS DAN PERTUMBUHAN Crocidolomia pavonana Fabricius Tamii, Amalia Fildzah; Rustama, Mia Miranti; Melanie, Melanie
Biotika Vol 12, No 1 (2014): BIOTIKA JUNI 2014
Publisher : Biologi Universitas Padjadjaran

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Penelitian ini bertujuan untuk mendapatkan formulasi HaNPV dengan jenis bahan pembawa yang menyebabkanmortalitas tertinggi, waktu kematian tercepat dan pertumbuhan berat larva Crocidolomia pavonana terendah.Helicoverpa armigera Nuclear Polyhedrosis Virus subkultur (HaNPV1) merupakan jenis MNPV yang dihasilkandari proses subkultur pada larva S. litura. Helicoverpa armigera Nuclear Polyhedrosis Virus subkultur (HaNPV1)diketahui berpotensi untuk mengendalikan populasi beberapa serangga hama. Akan tetapi HaNPVsangatrentan terhadap sinar ultra violet maka untuk menjaga efektifitasnya, HaNPV diformulasi dalam berbagai bahanpembawa seperti aquades, tepung maizena, tepung kanji, talk, gipsum dan zeolit. Metode penelitian yang digunakanadalah metode deskriptif eksplorasi dengan uji hayati. Kerapatan polihedra untuk masing-masing formulasi sebesar4x107 PIB/ml atau 4x107 1PIB/gram serbuk. Kemudian, larva C. pavonana instar dua diinfeksi virus secara oral.Hasil penelitian menunjukkan bahwa formulasi HaNPV dalam semua bahan pembawa menyebabkan mortalitas C.pavonana saat stadium larva mencapai 59,03% - 69,24% dan mortalitas C. pavonana saat stadium pupa mencapai14,24%- 22,36% serta lolos hidup larva C. pavonana menjadi imago mencapai 14,36%-26,07%. Adapun beratbadan maksimum larva C. pavonana yang terinfeksi HaNPV1 sebesar 0,059 gram/ekor-0,0747 gram/ekor, denganberat maksimum larva terendah yaitu sebesar 0,059 gram/ekor terjadi pada larva yang diinfeksi oleh formulasi HaNPV1 1dalam talk. Sedangkan berat pupa C. pavonana yang terinfeksi HaNPV berkisar 0,039-0,0427 gram/ekorlebih rendah dari berat pupa kontrol yaitu sebesar 0,0442 gram/ekor.
EFEKTIVITAS FORMULASI Helicoverpa armigera NUCLEAR POLYHEDROSIS VIRUS (HaNPV) HASIL SUB-KULTUR TERHADAP BERAT MAKSIMUM DAN LOLOS HIDUP LARVA Plutella xylostella Hardiani, Fuji; Melanie, Melanie; Rustama, Mia Miranti
Biotika Vol 12, No 1 (2014): BIOTIKA JUNI 2014
Publisher : Biologi Universitas Padjadjaran

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Penelitiaan ini bertujuan untuk mencari formulasi yang dapat menurunkan angka lolos hidup dan berat badanmaksimum terendah dibandingkan kontrol pada larva Plutella xylostella. Kerapatan polyhedra untuk masingmasingformulasisebesar4x107 PIB/ml atau 4x10 PIB/gram serbuk. Metode yang digunakan pada penelitian iniadalah metode deskriptif eksplorasi dengan uji hayati. Pada penelitian ini terdapat tujuh perlakuan yaitu kontrol(Virus 0 PIB/ml), formulasi HaNPV17 dalam bahan pembawa cair, formulasi HaNPV1 dalam bahan pembawa gipsum, tepung kanji, tepung maizena, talk, dan zeolit. Larva yang digunakan pada penelitian ini merupakan larva instar 2 yang diinfeksi secara oral. Hasil penelitian ini menunjukkan berat badan maksimum yang dapat dicapailarva P.xylostella berkisar antara 0.00357 gram/ekor – 0.00516 gram/ekor dengan berat badan maksimum terendahsebesar 0.00357gram/ekor pada larva yang terinfeksi HaNPV dalam formulasi cair. Angka lolos hidup Plutellaxylostella berkisar anatar 18% (pada perlakuan formulasi gipsum dan talk) hingga 50% (pada perlakuan formulasizeolit).
EFEKTIVITAS FORMULASI Helicoperva armigera NUCLEAR POLYHEDROSIS VIRUS HASIL SUB KULTUR (HaNPV ) TERHADAP MORTALITAS, WAKTU KEMATIAN DAN BERAT BADAN LARVA Spodoptera litura Fabricius (1775) Fitriyani, Fitriyani; Rustama, Mia Miranti; Melanie, Melanie
Biotika Vol 12, No 2 (2014): BIOTIKA DESEMBER 2014
Publisher : Biologi Universitas Padjadjaran

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Penelitian mengenai efektivitas formulasi Helicoperva armigera Nuclear Polyhedrosis Virus hasil sub kultur(HaNPV) dalam berbagai bahan pembawa (cair, gipsum, kanji, maizena, talk dan zeolit) yang diuji terhadapmortalitas, waktu kematian dan berat badan larva Spodoptera litura Fabricius telah dilakukan. Penelitian inimenggunakan metode desktiptif eksplorasi dengan uji hayati menggunakan S. litura Fabricius instar dua. Semuaformulasi HaNPV1PIB/mL/gr serbuk. Infeksi semuaformulasi virus diberikan pada setiap larva secara oral pada daun kubis. Hasil penelitian menunjukkan bahwaformulasi HaNPV1 1mengandung polihedra virus dengan kerapatan 4×107 dalam bahan pembawa cair, gipsum, kanji dan zeolit masing­masing dapat menyebabkanmortalitas pada larva S. litura sebesar 100%, tidak berbeda dengan formulasi HaNPV dalam bahan pembawamaizena dan talk (95%). Waktu kematian larva S. litura yang diinfeksi oleh formulasi HaNPV1berkisar antara5,55 ­ 8,62 hari. Adapun waktu kematian tercepat 5,55 hari terjadi pada larva yang diinfeksi virus dalam formulasicair. Rata­rata pertambahan berat badan larva S. litura yang diinfeksi HaNPVdalam bahan pembawa cair, gipsum,talk dan zeolit menurun berkisar antara 0.000662 ­ 0.00113 gr/ekor/hari dari berat badan larva kontrol denganpersentase 5.04 – 44.37%.Hasil penelitian menunjukkan bahwa HaNPV11 yang diformulasikan dalam berbagaibahan pembawa efektif untuk mengendalikan hama larva S. litura
Effectiveness of Storage Time Formulation of Bacillus Thuringiensis Against Aedes aegypti Larvae (Linnaeus, 1757) ., Melanie; Rustama, Mia Miranti; Sihotang, Inriyani Sintia; Kasmara, Hikmat
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

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Aedes aegypti is known as the main vector of dengue fever in Indonesia which causes a high number of deaths and become an outbreak every year. This disease is generally controlled by breaking the life cycle of mosquitoes with restricted breeds and using synthetic insecticides. So far biological agents from natural enemies and natural pathogens have been developed to anticipate the negative effects of synthetic insecticides. Bacillus thuringiensis is well known as entomopathogenic bacteria that can control various insect pests of cultivated plants and vector insects, such as Ae.aegypti.  B. thuringiensis can form endospores that produced toxin to Ae.aegypti, while the advatages are safe against non-target organisms such as natural predators and parasites, safe for mammals and humans and do not pollute the environment. Effective duration of B. thuringiensis in infecting Ae. aegypti is an important thing to be studied, moreover it is important to know the effectiveness of the expired formulation on the market and the residual effect. This ensures that the use of B. thuringiensis remains safe and does not create new problems such as Ae. aegypti  resistance. The experimental research was used bioassay method, toxicity test was performed B. thuringiensis expired and non expire as comparison at concentration 0 ppm (control), 0,3 ppm; 0.1 ppm; 0.07 ppm; 0.05 ppm; 0.03 ppm; 0.01 ppm. The parameters observed were mortality for 48 hours of toxicity test that results were indicated by LC 50, LC90 and recidual values. The results obtained were LC50 and LC formulations B.thuringiensis expired 0.305 ppm and 1.513 ppm much higher than the non expire LC50 and LC formulations of 0.0363 ppm and 0.8971 ppm. The recidual effect of expired B. thuringiensis on larvae Ae. Aegypti was only effective until day 12, whereas B. thuringiensis  that  non expire formulation was effectively until day 14.Keywords : Ae. aegypti, B. thuringiensis, mortality, recidual values, expired formulation
Effectiveness of Storage Time Formulation of Bacillus Thuringiensis Against Aedes aegypti Larvae (Linnaeus, 1757) Melanie, Melanie; Rustama, Mia Miranti; Sihotang, Inriyani Sintia; Kasmara, Hikmat
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar | Full PDF (600.668 KB)

Abstract

Aedes aegypti is known as the main vector of dengue fever in Indonesia which causes a high number of deaths and become an outbreak every year. This disease is generally controlled by breaking the life cycle of mosquitoes with restricted breeds and using synthetic insecticides. So far biological agents from natural enemies and natural pathogens have been developed to anticipate the negative effects of synthetic insecticides. Bacillus thuringiensis is well known as entomopathogenic bacteria that can control various insect pests of cultivated plants and vector insects, such as Ae.aegypti.  B. thuringiensis can form endospores that produced toxin to Ae.aegypti, while the advatages are safe against non-target organisms such as natural predators and parasites, safe for mammals and humans and do not pollute the environment. Effective duration of B. thuringiensis in infecting Ae. aegypti is an important thing to be studied, moreover it is important to know the effectiveness of the expired formulation on the market and the residual effect. This ensures that the use of B. thuringiensis remains safe and does not create new problems such as Ae. aegypti  resistance. The experimental research was used bioassay method, toxicity test was performed B. thuringiensis expired and non expire as comparison at concentration 0 ppm (control), 0,3 ppm; 0.1 ppm; 0.07 ppm; 0.05 ppm; 0.03 ppm; 0.01 ppm. The parameters observed were mortality for 48 hours of toxicity test that results were indicated by LC 50, LC90 and recidual values. The results obtained were LC50 and LC formulations B.thuringiensis expired 0.305 ppm and 1.513 ppm much higher than the non expire LC50 and LC formulations of 0.0363 ppm and 0.8971 ppm. The recidual effect of expired B. thuringiensis on larvae Ae. Aegypti was only effective until day 12, whereas B. thuringiensis  that  non expire formulation was effectively until day 14.Keywords : Ae. aegypti, B. thuringiensis, mortality, recidual values, expired formulation