Articles
2
Documents
Phytochemical Assay and Antiplatelet Activity of Fractions of Velvet Bean Seeds (Mucuna pruriens L.)

HAYATI Journal of Biosciences Vol 17, No 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (42.207 KB)

Abstract

Platelet aggregation is an important factor contributing to the formation of thrombus due to an uncontrolled blood clotting. An antiplatelet agent is a compound which decreases platelet aggregation and inhibits thrombus formation. The objectives of this study were to determine the class of compound employing phytochemical assay and to determine the in vitro antiplatelet activity of four fraction, namely hexane, ethyl acetate, butanol, and water fractions of velvet bean seeds (Mucuna pruriens L.) using epinephrine (EPN) as agonist of platelet aggregation. The antiplatelet activities were tested in human platelet rich plasma with hyperaggregation. To determine the activities, EPN was arranged at 4 level of concentrations  (300, 150, 75, and 30 μM), and antiplatelet agents were at 500 µg/ml. The results indicated that ethyl acetate, butanol and water fraction contained high flavonoids and moderate phenols. The water, butanol and ethyl acetate fractions of velvet bean seeds exhibited potential inhibition of EPN-induced platelet aggregation at all concentrations. The strongest antiplatelet agent was water fraction and had the same antiplatelet activity as aspirin at level 150, 75, and 30 μM of EPN. Butanol fraction had the same antiplatelet activity as aspirin at the lowest EPN (30 μM).

The Antiplatelet Aggregation Effect of Extract And Ethyl Acetate Fraction of Velvet Bean Seed (Mucuna pruriens L.) in Dyslipidemic Rat

Agritech Vol 31, No 1 (2011)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Original Source | Check in Google Scholar

Abstract

Cardiovascular disease (CVD) is the first cause of death in the world, CVD has complex and multifactorial process including atherogenic lipoprotein, oxidized low density lipoprotein (LDL), endothelial dysfunction, plaque stability, vascular inflammation, thrombotic and fibrinolytic disorder, exercises and genetic factor. Inhibiting the platelet ag- gregation is one of the CVD prevention. Velvet bean seed (Mucuna pruriens L.) can be found abundantly in Indonesia, but has not been used as herbal medicine. Ethanol extract and ethyl acetate of velvet bean seed contain high flavonoids and antioxidants properties which is expected could inhibit platelet aggregation. The objectives of the research were to determine the activity of ethanol extract and ethyl acetate fraction of velvet bean seed towards clotting and bleeding time in dyslipidemic rats. This research used completely randomized design in dyslipidemic rats which were given by ethanol extract of velvet bean seed at the concentrations of 50, 100 and 200 mg/kg BW/day and ethyl acetate fraction of velvet bean seed at the concentrations of 15, 30 and 60 mg/kg BW/day and 42.2 mg/kg BW/day aspirin for ten days. Clotting and bleeding time were measured at days 0, 10, and 20. Data were analyzed using One way analysis of vari- ance and continued with Duncan’s post Hoc test with 95 % level of significancy. The results showed that administration of 60 mg/kg BW/day ethyl acetate fraction of velvet bean seed and at the concentrations of 100 and 200 mg/kg BW/ day ethanol extract of velvet bean seed, prolong the clotting time at day 10, ethyl acetate fraction at the concentration of 60 mg/kg BW/day, 200 mg/kg BW/day ethanol extract of velvet bean prolong bleeding time at day 10.