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Pengaruh Pepton dan Waktu Inkubasi terhadap Produksi Inulinase oleh Pichia alni DUCC-W4 Berbahan Dasar Tepung Umbi Dahlia (Dahlia variabilis Willd.)

Bioma Vol. 09, No. 2, Tahun 2007
Publisher : Bioma

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Abstract

Sumber pemanis alami alternatif yang aman bagi kesehatan dapat diproduksi dari inulin dalam umbi dahlia(Dahlia variabilis Willd.) dan dapat dihidrolisis dengan inulinase dari Pichia alni DUCC-W4. Peningkatan produksiinulinase dapat dilakukan dengan menambahkan sumber nitrogen organik berupa pepton ke dalam medium. Hasilpenelitian menunjukkan aktivitas inulinase tertinggi sebesar 1,237 IU/mL (P2T3), aktivitas invertase tertinggi sebesar1,568 IU/mL (P2T3) dan aktivitas katalitik inulinase sebesar 0,824 IU/mL (P2T2) diperoleh melalui rasio S/I denganmembandingkan aktivitas invertase (S) dan aktivitas inulinase (I). Kesimpulan dari penelitian ini adalah penambahanpepton dengan berbagai konsentrasi (0%; 0,5% dan 1%) dan lama waktu inkubasi (12 jam, 18 jam dan 24 jam)tidak meningkatkan produksi inulinase Pichia alni DUCC-W4.

ISOLASI, UJI AKTIVITAS, DAN OPTIMASI INHIBITOR α-AMILASE ISOLAT KAPANG ENDOFIT TANAMAN BINAHONG (Anredera cordifolia) (Ten.) Steenis

Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Diabetes mellitus (DM) is a hyperglycemia disease caused by insensitivity of cells to insulin that causes the presence of glucose in blood and urine. The α-amylase inhibitors is a compound that inhibit carbohydrates breakdown into glucose by amylase enzyme. Binahong (Anredera cordifolia) has been used by Indonesian people since long time ago to treat various diseases such as for anti-diabetic. The aims of study were to obtained the endophytic fungal isolated from Binahong plant, and find out of their ability of α-amylase inhibitor activity by treatment of carbon source and pH of production medium. The isolating endophytic fungal from binahong roots, stems, and leaves in medium PDA. The α-amylase inhibitory test was using starch substrate and the α-amylase inhibitory measure using spectrophotometric method. The study was used one factor CRD i.e. carbon source including sucrose, lactose, and maltose. The best carbon source then continued to test with variation pH 5,6, and 7. The data obtained were then analyzed by Analysis of Variance (ANOVA). Five endophytic fungal isolated from binahong were only one isolated i.e DT should high potensial in inhibiting α-amylase 91,43%. The best carbon source for inhibitory α-amylase i.e sucrose with the percentage of 58,91%, while pH 5 is the best pH medium production continous treatment carbon source sucrose with the percentage 37,83%. The result of statistical test showed that sucrose as the best carbon source just than lactose and maltose, and pH 5 as the best medium pH for the production α-amylase inhibitor. Key words : α-amylase inhibitor, endophyte fungi, Anredera cordifolia, diabetes mellitus

ANALISIS MIKOFLORA DALAM MAKANAN FERMENTASI TRADISIONAL KEMPONG DI DESA KARANGPUCUNG KIDUL, LINGGAPURA BUMIAYU JAWA TENGAH

Jurnal Akademika Biologi Vol. 2 No. 1 Januari 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Kempong is a traditional fermented foods which is traditionally made of palm kernel cake substrate from the South Karangpucung Linggapura Bumiayu village, Central Java. This study is aimed to identify a mold which has a role in the fermentation process and testing in an activity of Kempong’s enzyme from mycoflora obtained. Proximate analysis of the samples of mold and palm kernel cake are conducted to determine the nutrient content of the substrate and fermentation products. The Results isolation, from the environment, substrate, laru and product, show 14 isolates of  molds, there are R.oryzae, A. niger Van Tieghem, A. carbonarius, Geotrichum candidum, A. ochraseus, Rhizomucor sp, A. chevalieri, A. tamarii, A. oryzae, A. flavus, A. nidulans, A. fumigatus, A. glaucus, and A. parasiticus. R. oryzae is a mold found in every material examined. It indicates that the fermentation is done mainly soothers by    R. oryzae. Proximate analysis of the kempong, shows a levels of carbohydrate 16.67%, 74.03% water, 0.75% ash, 2.80% fat and 5.77% protein. Nutrients content except water are lower than the substrate palm kernel cake. Decreasing of protein, fat, and carbohydrate fermentation are caused by R. oryzae. Keywords: kempong, mycoflora, enzyme activity, proximate analysis.

PENGARUH CaCl2.2H2O DAN WAKTU INKUBASI TERHADAP PRODUKSI INULINASE OLEH Pichia manshurica DUCC Y-015 DALAM SUBSTRAT TEPUNG UMBI DAHLIA

Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Fructose production from inulin by inulinase only need one reaction enzimatic step and produce 95% fructose. Inulin obtained from dahlia tuber and inulinase produced by Pichia manshurica DUCC Y-015. Inulinase production (E.C. 3.2.1.7.) can be influenced by metal salt suplementation, such as CaCl2.2H2O. The purpose of this research were to known the influence of CaCl2.2H2O and incubation time to inulinase production by P. manshurica DUCC Y-015 on Dahlia Tuber Substrate. The design that use in this research were Randomized Factorial Block Design ( RAFBD ). Factor I (CO, C­1, C2, C3) as the concentration of CaCl2.2H2O (0 mM, 0.25 mM, 0.50 mM, 1.00 mM) and Factor II ( T12, T18, dan T24 ) as incubation time ( 6, 12, 18 hour), the repetition were 3 times. The result analyze by ANOVA (Analysis of Variance) and continued by LSD test. The result of this research indicate that CaCl2.2H2O and incubation time were not significantly influence to inulinase production. The highest inulinase production by Pichia manshurica DUCC Y-015 indicate by C2T12 treatment which use 0.50 mM CaCl2.2H2O and 12 hour incubation time, the enzyme activity is 0.60 IU/mLKey Words : CaCl2.2H2O, Dahlia tuber,  Incubation time, Inulin, Inulinase, Pichia manshurica DUCC Y-015.

KEANEKARAGAMAN DAN AKTIVITAS ENZIMATIS KAPANG RIZOSFER KACANG MEONGAN (Aeschynomene americana L.) DI DESA SUKOLILO BARAT, KECAMATAN LABANG, KABUPATEN BANGKALAN, MADURA

Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Molds are widely distributed in nature, they even present in extreme environment, such as hot and dry soil. Molds which can grow in extreme environment has been adapted to xeric environment by producing enzymes  with  special  characteristics.  The  aim  of  this  study  to  determine  the  diversity  of  molds  from A.americana L. rhizosphere at West Sukolilo Village, Labang district, Bangkalan Regency, Madura and also to examined their cellulolytic and proteolytic activities. Isolation of molds were done by using spread and dilution methods. Molds identification were done by macroscopic and microscopic examined. The molds diversity was calculated using Shannon-Wiener index. Semiquantitative enzyme examination were done using Carboxymethil Cellulose (CMC) agar for cellulolytic ang liquid gelatin 12% for proteolytic. Results of this study showed that mold diversity isolated from   A. americana L. rhizosphere was moderate (1,8-2,7) with total of 43 species, comes from 7 genus i.e. Aspergillus, Penicillium, Fusarium, Aureobasidium, Byssochlamys, Paecilomyces, and Trichoderma.  The  highest  index  of  cellulolytic  produced  by  Aspergillus  sydowii  (2,35),  while  highest proteolytic activity produced by Aspergillus flavus (86%). Keywords : molds, diversity index,xeric, cellulolytic, proteolytic

PRODUKSI SELULASE OLEH KAPANG Aspergillus sp. HASIL ISOLASI DARI LIMBAH PENGOLAHAN SAGU (Metroxylon sp.) DENGAN VARIASI KONSENTRASI INOKULUM PADA FERMENTASI TERENDAM STATIS

Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Sago processing waste is one of agro-industry waste, which contains cellulose 19,55% and lignin 25%. Genus Aspergillus has been widely known as cellulase-producing fungi. Isolation of Aspergillus from sago processing waste is expected to get a potential isolates indegenous cellulolytic fungi. Cellulase (EC 3.2.1.4) is an enzyme that can break down cellulose into glucose monomer form, with consists of three components that work in synergy are endoglucanase, eksoglucanase and β-glucoside. This study aims to obtain isolates of Aspergillus sp. cellulolitic potential from sago processing waste and cellulase production of the cellulolytic isolates in various concentrations of inoculum. The study was conducted using completely randomized design (CRD) 1 factor of 4 levels of inoculum concentration treatments were 0%, 2,5%; 5%; and 10% with three replications. The observed variables are biomass, CMCase activity and FPase activity. Data were analyzed by ANOVA with a significance level 95%. The result of isolation, two isolates of Aspergillus namely A. flavus and A. paradoxus with cellulolitic index respectively 2,63 and 2,06. Cellulase activity isolate of A. flavus either endoglucanase (CMCase) or total cellulase (FPase) are not affected by the concentration of inoculum.Keywords: cellulase, sago waste, Aspergillus, inoculum

Eksplorasi Jamur Alkalotoleran dari Desa Sukolilo Barat, Kecamatan Labang, Kabupaten Bangkalan, Madura, Jawa Timur

Bioma Vol. 16, No.1, Tahun 2014
Publisher : Bioma

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Abstract

Mold exploration from limestone hills West Sukolilo  village, District Labang, Bangkalan, Madura, East Java,  conducted to found the  alkalotolerant mold isolates and investigate their  amylolytic, proteolytic, and cellulolytic activity. Mold isolation were done using spread plate and dilution method  on  PDA and CMA at pH 8.0, 9.0, and 10.0. Twenty-seven mold isolates have been found consist of  four genus  i.e. Aspergillus, Fusarium, Curvularia, and Trichoderma. The result of amylolytic test  showed Trichoderma longibrachiatum (IMD-26) have the  highest activity at pH 8, while  Fusarium sp 2 (IMD-24) showed the highest activity at pH 9. Aspergillus flavus (IMD-7) showed highest proteolytic activity at pH 8, while Hyphomycete (IMD-27) showed the highest activity at pH 9. The highest cellulolytic activity at pH 8 showed by Trichoderma harzianum (IMD-25) and pH 9 by Fusarium sporotrichoides (IMD-20). Keyword : alkalotoleran mold, amylolytic, proteolytic, cellulolytic.

Diversitas Kapang Serasah Daun Talok (Muntingia calabura L.) Di Kawasan Desa Sukolilo Barat, Kecamatan Labang, Kabupaten Bangkalan, Madur

Bioma Vol. 16, No.2, Tahun 2014
Publisher : Bioma

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Abstract

Leaf litter is one kind of substrate which is good for mold’s growth because of its organic matters content particularly cellulose. Research on mold diversity on Talok (Muntingia calabura L.) leaf litter which grow abundance at Sukolilo Barat Village, Labang Subdistrict, Bangkalan, Madura was conducted in order to determined the diversity and to isolate xerophilic mold as well as to examined the enzymatic activity of the isolates. The isolation has been done by direct and indirect method on DG18 agar, MEA, and OA from three samples which is taken purposively. The enzyme activities observed were cellulolytic, amylolitic and proteolytic at 310C temperature. The results showed that 24 isolates found were come from 3 genus i.e. Aspergillus, Curvularia and Fusarium. The Aspergillus was the largest number found. The Shannon-Wiener Index of Diversity showed that the diversity of three leaf litter samples categorized medium. The highest cellulolytic, amylolitic and proteolytic activity at 310C incubation were showed by A. tamarii (ISM 1), A.aculeatus (ISM 10) , A. terreus sp. 3 (ISM 17) respectively. Keywords : Diversity, mold, talok litter leaf, Madura

Uji Kemampuan Produksi Fruktooligosakarida (FOS) dari Kelompok Aspergillus niger DUCC

Bioma Vol. 15, No.1, Tahun 2013
Publisher : Bioma

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Abstract

Prebiotic is a food supplement that can not be digested in the human’s gut, it can stimulate the growth of beneficial microorganisms in the intestine and improve human’s health. FOS is a fructan type of oligosaccharide which is have a potential as a natural prebiotic, it  can be produced by several microorganisms, including A. niger group. The aim of this research was to examined the FOS production of   three isolates of A. niger group from DUCC collection (i.e. DUCC F123, DUCC F129 and DUCC F102). FOS production was determined by measuring the reducing sugar using  DNS method. The result showed that all isolates have the capability in producing  FOS suspected kestose with the degree of polymerization 3.545; 3.215; 3.049 respectively.   Keywords:FOS, fructosyltransferase, Aspergillus niger, prebiotic

ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT PADA PANGAN FERMENTASI MANDAI

Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

East Kalimantan has a lot of processed food products traditionally, one of them is mandai which is fermented food made from fruit leather Cempedak (Arthocarphus champeden). Lactic acid bacteria involved in the fermentation mandai greatly affect the quality of the final product. This study aimed to perform the isolation and characterization of lactic acid bacteria in the mandai fermentation process. MRS medium was used in the isolation of bacteria for 14 days. Isolation of bacteria during the fermentation process mandai obtaining 17 isolates, nine isolates Gram negative isolates, and eight isolates Gram positive. Eight isolates showed positive results against some of the morphological and biochemical characterization. Including biochemical characterization, acid formation and gas production from glucose, motility test, catalase test, growth at 160C and 480C, hydrolysis of starch, fat hydrolysis, and hydrolysis of casein and acid formation test. Eight isolates bacteria made mandai into processed food products that had a sour taste. Keywords: lactic acid bacteria, BAL ,bacterial isolates, cempedak skin.