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ISOLASI DAN SELEKSI BAKTERI ASAM LAKTAT DARI YOGHURT PRODUKSI INDUSTRI RUMAH TANGGA DI PEKANBARU YANG BERSIFAT ANTIBAKTERI TERHADAP Escherichia coli DAN Salmonella typhi Yani, Lili; Roza, Rodesia Mustika; Martina, Atria
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

The occurance of Lactic Acid Bacteria (LAB) in yoghurt produced by home industry inPekanbaru as probiotic potentially improve intestinal physiological function, useful forhealth as well as prevention of the growth of pathogenic bacteria. The purposes of thisstudy were to calculate the total LAB population of yoghurt with the effect of mango andstrawberry juice during different storage process, and also to determine the ability ofantibacterial activity against Escherichia coli and Salmonella typhi. The population of LABin yoghurt was 4,0 × 10 7 – 2,9 × 10 8 CFU/ml. Antimicrobial activity assay used the welldiffusion method. The mango juice yoghurt incubated in 7 day was the most effective forEscherichia coli with the inhibition zone 27,1 mm. While the mango juice yoghurtincubated in 3 day was the most effective for Salmonella typhi with inhibition zone 29,9mm.
SELEKSI DAN UJI ANTIBAKTERI AKTINOMISETES ASAL TANAH GAMBUT RIMBO PANJANG KAMPAR RIAU TERHADAP Escherichia coli DAN Salmonella typhi Suryani, Siti; Roza, Rodesia Mustika; Martina, Atria
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Actinomycetes is one of microorganisms that can be  used as a natural antibiotic. Metabolites produced by actinomycetes have antagonistic activity on bacteria, therefore it can be used as antibacteria. The purposes of this study were to select and test the antibacterial activity of actinomycetes isolated from peat soil in Desa Rimbo Panjang Kabupaten Kampar Riau to inhibit the growth of Escherichia coliand Salmonella typhi. This study was conducted from December 2013-January2014 at the Microbiology Laboratory, University of Riau. Serial dilutions and pour plate method used for isolation of actinomycetes while agar disk method used for antibacterial test. The result found a total of 24 actinomycetes isolates, 10 isolates inhibiting  E. coli, and 16 isolates inhibiting S. typhi. The inhibition zone diameter of E. coliand S. typhi  were 8,3 to 16,9 mm and 7,8 to 12,9 mm, respectively.
ANALISIS FISIOLOGI JAMUR LIGNINOLITIK DAN SELULOLITIK ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR SEBAGAI AGEN BIOKOMPOS Larasati, Karina; Roza, Rodesia Mustika; Martina, Atria
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Cellulolytic and ligninolytic fungi have an important role in degradation of organic materials, especially lignin and cellulose, in a composting process. The success of the fungi in the composting process is strongly influenced by temperature and pH. The purpose of this research was to obtain isolates of the fungi with the best ability to be applied as biocompost agent by analyzing its ability to grow in different temperature and pH. A total of 110 isolates from peatsoil in Rimbo Panjang, Kampar Regency, Riau was inoculated into Potato Dextrose Broth medium with pH of 5 and pH of 7 then incubated for 3 days at room temperature (±27ºC), 40ºC, 50ºC, 60°C, 70ºC and 80ºC. The results obtained 110 isolates which were grown at room temperature (±27ºC) with pH of 5 and pH of 7 and 96 isolates which were grown at 40ºC temperature. A total of 27 isolates which were grown at 50ºC temperature consisted of genus Penicillium, Trichoderma, and Aspergillus.
UJI POTENSI ANTIFUNGI AKTINOMISETES SELULOLITIK DAN LIGNINOLITIK DAN BAKTERI LIGNOSELULOLITIK ISOLAT LOKAL TERHADAP PERTUMBUHAN JAMUR Ganoderma boninense DAN Colletotrichum capsici Martin, Dede; Martina, Atria; Roza, Rodesia Mustika
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Colletotrichum capsici fungi, causing agent of anthracnose in chili, and Ganoderma boninense, fungi causing agent of basal stem rotten in oil palm, can reduce the productivity of chili and oil palm. These disease are increasing so that it is necessary to find local biological agents that are environmentally friendly. The purpose of this research was to determine the potential isolates of lignocellulolyltic bacteria and cellulolytic and ligninolytic actinomycetes from peat soil of Rimbo Panjang Kampar, Riau as an antifungal agent to inhibit the growth of C. capsici and G. boninense.Antifungal activity was screened using agar disc method by measured the inhibition zone for seven days. The results showed that 13 isolates of actinomycetes haveantifungal activity against C. capsici with the highest inhibition zone 13,3 mm by RB2S40. Six isolates of actinomycetes have antifungal activity against G. boninense with the highest inhibition zone 29,15 mm by RB1S4. Five isolates have the ability to inhibit both fungi which were targeted.
Seleksi Potensi Bakteri Probiotik Indigenus Riau sebagai Agen Biopreservatif Bahan Pangan WULANDARI, NUR ASYIAH; FIBRIARTI, BERNADETA LENI; ROZA, RODESIA MUSTIKA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

The use of synthetic preservatives is not safe for consumption and very dangerous for health.Lactic acid bacteria (LAB) is a probiotic bacterium that can produce bacteriocins asbiopreservatives agents. Bacteriocins have been widely applied as a food preservative, but itscommercial availability is still low and very expensive. This study was conducted to test the abilityof 23 isolates of local probiotic bacteria in Riau to produce bacteriocins. LAB total population wascalculated by Total Plate Count (TPC) method. LAB inhibition test against indicator bacteriaStaphylococcus aureus FNCC 0047 was conducted using well diffusion method. Bacteriocinsantimicrobial confirmatory test was performed at 100OC for 15 minutes, 30 minutes, and 60minutes. The result showed that the highest LAB population was PSG4 (22.45 x 106 cfu/ml) andthe lowest was SLK2 (4.75 x 106 cfu/ml). The greatest inhibitory activity against indicator bacteriaS. aureus FNCC 0047 was found in PSG2 (15.1 mm) and the smallest was in SLK2 (10.75 mm)with the activity of bacteriocins 2575 mm2/ml and 792.6 mm2/ml respectively. The bacteriocinsconfirmatory test showed clear zone of isolates JN5, PSG2, and SLK2 with the largest inhibitionzone was during heating for 30 minutes and 60 minutes (19.2 mm, 19.5 mm, and 16.8 mm) whilethe smallest was for 60 minutes and 15 minutes (12.8 mm, 15.6mm, and 14.7 mm).Keywords: Probiotic bacteria, biopreservatives, bacteriocins, Staphylococcus aureus FNCC 0047.
EKSPLORASI DAN UJI DAYA HAMBAT BAKTERI ENDOFIT DARI TANAMAN BENALU SAWO (Helixanthera sp.), BENALU COKLAT (Scurulla sp.) DAN BENALU KOPI (Helixanthera sp.) TERHADAP Escherichia coli Walpajri, Febri; Roza, Rodesia Mustika; ', Fitmawati
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Endophytic bacteria are microscopic microorganisms that live in the tissues of plants such as leaves, roots, fruits and stems. The parasite plants on sapodilla (Helixanthera sp.), cocoa (Scurulla sp.) and coffee (Helixanthera sp.) are medicinal plants. Theseplants have endophytic bacteria that have antibacterial compounds as antibiotics that inhibit the growth of Escherichia coli. The purpose of this study was to get endophytic bacteria isolates from parasite plants on sapodilla, cocoa and coffee, and determine the antibacterial activity against E. coli. Sampling was conducted in Kampar regency and Pekanbaru city. The parasite plants were collected from the field and their endophytic bacteria  were selected using surface sterilization method and purified before their  activity against E. coli being tested. The selected bacteria were then characterized. The results  obtained  34  endophytic  bacterial  isolates  that  had  activity  against  E.  coli. Endophytic bacteria isolates which had the highest activity against E. coli isolates were  Bbs4 from sapodilla parasites with 12,1 mm inhibition zone diameter.
Antioxidant Activity of Dominant Plants Species in Obat Pahit from Lingga Malay Ethnic in Riau Archipelago Fitmawati, Fitmawati; Sofiyanti, Nery; Roza, Rodesia Mustika; Isnaini, Isnaini; Irawan, Yulisa Resti; Winata, Dhaniel Ridho; Dewi, Awal Prichatin Kusumo
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9808

Abstract

Obat Pahit is a potion that has been long commonly consumed by Lingga Malay society for generations as stamina keeper. The most dominant plants found in the packaging of the Obat Pahit were namely Bauhunia semibifida, Cnestis palala and Penawa Root (3 species). This research aimed to investigate and determine activity of antioxidant contents in Obat Pahit from five Traditional Medicine Practitioners (TMPs) in the district of Lingga. The tested samples were mashed then being soaked into 2 types of solvent: distilled water and methanol, containing HCl 1%. DPPH method was also used in this research. Quantitatively antioxidant activity test of Obat Pahit from the five TMPs by using methanol solvent had extremely highest activity compared to the distilled water solvent. The test, using TLC plate by spraying the extract from three dominant plants with 0.1 mM of DPPH solution, produced a pale-yellow spots at a wavelength of 366 nm. On the other hand, the test using HPLC at wavelengths of 230 nm and 280 nm showed the presence of two dominant secondary metabolites contents: flavonoid and phenolic. IC50 (ppm) of Bauhinia semibifida (6.6247), Penawa Root (5.0124) and Cnestis palala (5.9968) were much lower than IC50 of mangosteens rind (41.7675), vitamin C (6.6612) and Stimuno drug (8.333). This antioxidant analysis has not been reported previously. This proof contributed greatly to uncovering potentially native natural resources as an indigenous Indonesian drug which is expected to decrease dependence on imported drugs especially imunomodulator, antihypertensive, antidiabet etc. This research would be beneficial and excellent manifestation for the development of natural antioxidant-based medicines from traditional knowledge of Indonesias local ethnicities.
Immunomodulatory Effectiveness of Aqueous Obat Pahit Extract of Lingga Malay Ethnic on White Rats (Rattus novergicus) Fitmawati, Fitmawati; Roza, Rodesia Mustika; Sofiyanti, Nery; Isnaini, Isnaini; Fitri, Febrian Lailatul; Paramita, Desi; Dewi, Awal Prichatin Kusumo
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10496

Abstract

Obat pahit has been generally known and believed by Lingga Malay society as anti-aging agent. However, the study of Obat pahit is not scientifically proven. This research was aimed to prove immunomodulatory ability of Obat pahit potion from Lingga, Riau Archipelago. This study used white rats as an animal modelling, and Staphylococcus aureus as bacteria tester. The rats had been treated with aqueous Obat pahit extract from three TMPs on dose scales of 0.09, 0.18 and 0.27 mL/200g of body weight through oral administration for 7 days. Furthermore, on the 8th days, the experiment animals were injected by the preparation of bacteria tester through intraperitoneal administration in the amount of 0.5 mL/200 gram of body weigth and subsequently incubated for 1 hour after the injection. There were 2 observed parameters on this study, i.e efectivity and capacity of phagocytosis by leukocytes. The observation of leukocytes-phagocytocis activity was carried out by making a smear preparat samples of peritoneum fluid from rats. After the observation under microscope on a magnification of 100 times. The result was obtained the Obat pahit from Kalan PMT swere more effective on dose 2, while from SP4 and Linau TMPs were much more effective on dose 1. It is therefore, using these data of the results, the advanced doses scale of this Obat pahit would not be necessary. Obat pahit potion from Malay Lingga Malay Ethnic could become raw materials of immunomodulatory herbal medicine based on traditional knowledge. It also potentially as a standardized herbal.
Seleksi Isolat Aktinomisetes Asal Tanah Gambut Desa Rimbo Panjang Kabupaten Kampar Dalam Menghasilkan Hormon IAA (Indole Acetic Acid) MAWARTI, INDAH; FIBRIARTI, BERNADETA LENI; ZUL, DELITA; ROZA, RODESIA MUSTIKA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Hormon IAA (Indole Acetic Acid) termasuk fitohormon golongan auksin yang berperan sebagai zat pemacu pertumbuhan tanaman. Selain tumbuhan, mikroba juga diketahui mampu menghasilkan IAA. Penelitian ini bertujuan untuk menyeleksi kemampuan koleksi isolat aktinomisetes Laboratorium Mikrobiologi FMIPA UR dalam menghasilkan IAA. Uji produksi IAA oleh aktinomisetes dilakukan dengan penambahan reagen Salkowski menggunakan metode kolorimetri dalam medium SCB (Starch Casein Broth) yang diperkaya dengan triptofan sebagai prekursor dan tanpa triptofan. Hasil penelitan ini diperoleh sebanyak 50 dari 85 isolat aktinomisetes yang memiliki kemampuan dalam menghasilkan hormon IAA. Produksi IAA tertinggi dihasilkan oleh RB5S78 sebesar 35,97 ppm dan konsentrasi terendah dihasilkan oleh RB4S67 sebesar 0,24 ppm dalam medium SCB yang diperkaya triptofan. Pada medium tanpa triptofan hasil tertinggi diperoleh isolat aktinomisetes RB5S78 dengan konsentrasi sebesar 15,28 ppm dan konsentrasi terendah dihasilkan oleh RB4S65 sebesar 0,16 ppm. Isolat aktinomisetes yang menghasilkan IAA dengan kriteria tinggi yang diperoleh termasuk dalam genus Streptomyces dan Rhodococcus.
Penerapan teknologi hidroponik sistem deep flow technique sebagai usaha peningkatan pendapatan petani di Desa Sungai Bawang Fitmawati, Fitmawati; Isnaini, Isnaini; Fatonah, Siti; Sofiyanti, Nery; Roza, Rodesia Mustika
Riau Journal of Empowerment Vol 1 No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

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Abstract

Target menjadi desa mandiri harus dibarengi dengan penguatan kemampuan desa dalam memenuhi kebutuhan dasar secara mandiri seperti pangan. Sayuran merupakan kebutuhan pangan penting untuk mencapai masyarakat sehat sejahtera. Kebutuhan sayuran di Desa Sungai Bawang, Kabupaten Kuantan Singingi, terkendala lahan pertanian yang tidak mendukung untuk bercocok tanam sayur-sayuran. Sistem penamanan secara hidroponik menawarkan teknik tanam tanpa tanah yang dapat diterapkan dimanapun. Hidroponik mempunyai nilai tambah berupa hasil produksi yang lebih bersih dan higienis. Teknologi hidroponik sistem Deep Flow Technique mampu menyediakan air dan oksigen bagi tanaman dan cocok untuk menanam sayuran. Hidroponik ini sesuai diterapkan di Desa Sungai Bawang dan tidak memerlukan lahan luas dalam bercocok tanam untuk mengaplikasikan teknologi ini. Strategi pendampingan dilakukan dengan penyuluhan dan demonstrasi tahap-tahap penanaman secara langsung dan praktek langsung oleh masyarakat. Kegiatan ini memberikan pengaruh positif bagi masyarakat serta banyak warga masyarakat yang ingin menerapkan teknologi hidroponik. Ini merupakan langkah awal dalam rangka meningkatkan pendapatan masyarakat desa khususnya petani. Untuk masyarakat umum, teknologi ini memudahkan masyarakat memperoleh sayur untuk dikonsumsi dengan harga terjangkau.