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ISOLASI DNA DAN AMPLIFIKASI FRAGMEN D-LOOP MITOKONDRIAL PADA IKAN Ompok hypophthalmus (Bleeker, 1846) DARI SUNGAI KAMPAR PROVINSI RIAU Rinarta, Della; Elvyra, Roza; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Total DNA molecule and fragment of the mitochondrial D-loop region is needed to determine the nucleotides sequence of mitochondrial D-loop region from lais danau fish (Ompok hypophthalmus). Therefore, this study aimed to isolate and amplify mitochondrial D-loop region that can be used as genetic information resources of O. hypophthalmus. The samples of O. hypophthalmus were taken from flood plain river in Riau, Kampar River. Tail muscle of O. hypophthalmus was used for DNA isolation. DNA molecules were amplified using PCR machine with L15926 and H00651primers. The total DNA of O. hypophthalmus was obtained in this study. The amplification process produced 153 bp fragment mitochondrial D-loop region of O. hypophthalmus.
KROMOSOM STICKY PADA KECAMBAH PADI (Oryza sativa L.) ASAL KABUPATEN BENGKALIS, RIAU TERKAIT CEKAMAN GARAM Ningsih, Ermi; Roslim, Dewi Indriyani; Sofiyanti, Nery
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Rice (Oryza sativa L.) is a plant that is not resistant to the salt stress, but the development of agriculture in coastal areas requires the analysis of rice genotypes that are resistant to the salt stress. The salt stress may cause chromosomal abnormality, such as sticky chromosome. This study was aimed to analyze the influence of the salt stress on the formation of chromosomes in four rice genotypes originating from Bantan Air village, Bengkalis. This research was conducted using randomized block design (RBD) with two factors, i.e. NaCl concentrations (0 mM, 60 mM, 75 mM, and 90 mM) and the rice genotypes (IR64, Indragiri, Solok, Sadani, Yamin, and Amat Candu) with three replications for each treatment. Normal anaphase chromosomes were visible in control, whereas sticky chromosomes were seen in 90 mM salt exposure. It concluded that salt stress may cause sticky chromosomes.
DESKRIPSI KARAKTER MORFOLOGI UBI KAYU (Manihot esculenta Crantz) JURAY DARI KABUPATEN ROKAN HULU Caniago, Murtiana; Roslim, Dewi Indriyani; ', Herman
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Cassava is an alternative food to substitute rice as a staple food. The improvement of cassava in breeding program requires many high genetic diversity of cassava. The objective of this study was to describe the morphological characters of cassava cv. Juray from Kabupaten Rokan Hulu, Riau. This research was conducted at Biology Department, FMIPA UR, for 12 months, started from March 2013 to February 2014. The cassava stems were planted and then observed on fifth and twelfth months. The results showed that cassava cv. Juray had morphological characters as follows: the stem color was grey, the stem diameter was large (3.4 cm), the stem texture was grooved, the petiole length was medium (9.77), the flowering time was 10 months after planting, the tuber shape was irregular, the external color of tuber was light brown, the tuber cortex thickness was thin (1 mm), the tuber cortex color was cream, and the root pulp color was white.
TEKNIK ISOLASI DAN ELEKTROFORESIS DNA TOTAL PADA Kryptopterus apogon (Bleeker 1851) DARI SUNGAI KAMPAR KIRI DAN TAPUNG HILIR KABUPATEN KAMPAR PROVINSI RIAU Novitasari, Dede Aryani; Elvyra, Roza; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Kryptopterus apogon is one of freshwater fish species that was only found in floodplain river in Riau Province. Many species of lais fish are found in Riau Province rivers and morphologically has the same shape therefore it is necessary to find alternative markers to distinguish the species. The important step in genetic analysis is DNA isolation. This study aimed to isolate the total DNA from Kryptopterus apogon. The fish samples were taken from the Kampar Kiri and Tapung Hilir rivers. Isolation of total DNA was done by taking the muscle tissue from the fish tail (1 mm x 1 mm in size). Isolation of total DNA was done using DNA isolation kit (QIAGEN DNeasy Blood & Tissue). After that, the total DNA was checked on 1% agarose gel to estimate the quantity and quality of total DNA. Total isolated DNA of Kryptopterus apogon from two rivers had various quality and quantity. Tha total quantity of DNA molecules ranged from to 100-200 ng/μl, while the total quality was good enough or not fragmented. The DNA was clear, thick, and no smear.
ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Akmando, Rizka Suci; Elvyra, Roza; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

JOM FMIPA Volume 1 No. 2 Oktober 2014 224 ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Rizka Suci Akmando, Roza Elvyra, Dewi Indriyani RoslimMahasiswa Program S1 Biologi Dosen Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Kampus Bina Widya Pekanbaru, 28293, Indonesia rizkasuciakmando@yahoo.co.idABSTRACT Scientific information of mtDNA D-loop region in fish, Kryptopterus limpok, from Riau is unknown. Before having genetic information, total DNA isolation and amplification is necessary to be carried out. This study was aimed to obtain the total DNA molecules and DNA fragment of the mtDNA D-loop region in fish K. limpok from Sungai Kampar Kiri, Riau. Ten muscle samples were taken from fish collected from the river and used for total DNA isolation. Products of DNA isolation were amplified using the PCR technique with universal primers i.e. L15926 (F) 5’ TCA AAG CTT ACA CCA GTC TTG TAA ACC 3’ dan H00651 (R) 5’ TAA CTG CAG AAG GCT AGG ACC AAA CCT 3’. The PCR process consisted of pre-PCR at 940C for 5 minutes, 35 cycles of PCR consisted of three stages, i.e. as denaturation at 940C for 30 seconds, annealing at 590C for 1 minutes, elongation at 720C for 1 minutes, and post-PCR at 720C for 5 minutes. This study obtained the intact total DNA molecules, with the total DNA concentration of 100 μg/ μl. The length of mtDNA D-loop fragment was 153 bp.
JOM FMIPA Volume 1 No. 2 Oktober 291 PERTUMBUHAN CACING TANAH (Perionyx sp) PADA DUA MEDIA Manurung, Rotupa Juliana; ', Yusfiati; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

This research was conducted to determine the growth of earthworm ( Peryonix sp) in two types of media, that is mixture of palm sheath waste with chicken manures and mixture of vegetables waste with chicken manures. This research was conducted at Biological Garden Department of Biology Math and Science Faculty Riau University. The research used completely randomized design with five treatments and three replications i.e treatments M1 (100% palm sheath waste); M2 (75% palm sheath waste + 25% chicken manure); M3 (50% palm sheath waste + 50% chicken manure); M4 (25% palm sheath waste + 75% chicken manure) and M5 (100% chicken manure), S1 (100% vegetables waste); S2 (75% vegetables waste + 25% chicken manure); S3 (50% vegetables waste + 50% chicken manure); S4 (25% vegetables waste + 75% chicken manure) and S5 (100% chicken manure). Analysis of variance showed that type of media had significantly affected body weight and amount of coccon produced. The biggest body weight and amount coccon were at media M2 and S3 in the day of 20.
ISOLASI DNA TANAMAN PADI (Oryza sativa L.) ASAL KECAMATAN BANTAN, BENGKALIS – RIAU. Deanesia, Dita; Roslim, Dewi Indriyani; ', Herman
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Excess  of  Fe  in  costal  land  in  Riau Province  is potential to  poison the  roots  of  plants. However,the plants have their  own  mechanism  to overcome  the Fe overload.  This  study was aimed  to  isolate  the total   DNAof rice plants among  three  local  rice genotypes  from Riau  which are  commonly  planted in costal  land  in  Bengkalis,  Riau, with  Fe overload resistant  swamp  rice variety  (Mahsuri)  and Fe overload sensitive rice variety  (IR64). The experiment was conducted  in the Laboratory of Genetics, FMIPA UR,from January to May   2014. Research methods  consisted of  isolation  of DNA  and  electrophoresis.  In this study  isolation of DNA  from five rice  varieties  was succesfully done. Among those DNA, concentration  of  total DNA  which were  obtained from IR64 and Amat Candu were very high.
KROMOSOM STICKY PADA PADI (Oryza sativa L.) ASAL BENGKALIS TERHADAP CEKAMAN ALUMINIUM (Al) Lestari, Endah Budi; Roslim, Dewi Indriyani; ', Herman
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Bengkalis has various soil types, one of them is acid soil. The major problem in acid soil is aluminium toxicity that can harm rice plant roots. The objective of this study was to identify sticky chromosomes in some local rice genotypes from Desa Bantan Air, Bengkalis, Riau Province grown in Al stress medium. Plant materials examined were four local rice genotypes from the Desa Bantan Air, i.e. Amat Candu, Sadani, Solok, and Yamin. All of the rice genotypes were exposed to 12 ppm Al for 72 hours in minimum nutrient culture, pH 4. Each treatment was repeated three times. Randomized Block Design was used in this research. The results showed that sticky choromosomes were only detected at Yamin genotype treated by 12 ppm Al. It concluded that the sticky chromosomes found in the local rice genotypes from Bengkalis was Yamin genotype
DISAIN PRIMER AKTIN SPESIFIK TUNTUN ANGIN (ELAEOCARPUS FLORIBUNDUS) (ACTIN PRIMER DESIGN SPECIFIC OF TUNTUN ANGIN (ELAEOCARPUS FLORIBUNDUS)) Roslim, Dewi Indriyani; Herman, Herman
JURNAL BIOS LOGOS Vol 7, No 1 (2017): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.7.1.2017.16207

Abstract

Abstrak Aktin merupakan salah satu dari housekeeping gene yang dapat digunakan sebagai kontrol internal pada analisis ekspresi gen.  Sampai saat ini, sekuen DNA dari gen penyandi aktin pada tanaman tuntun angin (Elaeocarpus floribundus) belum pernah dipublikasikan. Penelitian ini bertujuan merancang primer aktin spesifik untuk tanaman Elaeocarpus floribundus bedasarkan primer aktin degenerate.  Pasangan primer degenerate rancangan yang digunakan adalah PlAc46S sebagai primer forward: 5?- ATGGTNGGNATGGGNCARAA-3? dan PlAc245N sebagai primer reverse: 3?- GTDATNACYTGNCCRTCNGG-5?.  Metode penelitian meliputi isolasi DNA dari daun segar, amplifikasi dengan teknik polymerase chain reaction (PCR), elektroforesis pada 1,2% gel agarose, perunutan nukleotida, dan analisis data menggunakan program BLASTn dan perangkat lunak MEGA versi 6.06.  Fragmen DNA aktin telah berhasil diamplifikasi menggunakan primer aktin degenerate dan telah dirancang primer aktin spesifik untuk Elaeocarpus floribundus dengan urutan sebagai berikut:5?-CCAAATCATGTTTGAGACCT-3?(forward) dan 5?-GAACACGTTAATTCCTGCTC-3? (reverse). Primer tersebut selanjutnya dapat digunakan untuk mengamplifikasi gen aktin dari E. floribundus pada analisis ekspresi gen.Kata Kunci: aktin, Elaeocarpus floribundus, PCR, primer degenerate, tuntun angin. Abstract Actin is one of the housekeeping genes which commonly be used as an internal control in gene expression analysis.  Until now, the DNA sequence of actin gene of tuntun angin (Elaeocarpus floribundus) has never been published. This study aimed to design the actin primer specific for E. floribundus based on degenerate actin primer.  The pair of the degenerate actin primers (PlAc46S 5?- ATGGTNGGNATGGGNCARAA-3? and PlAc245N 3?- GTDATNACYTGNCCRTCNGG-5?) were used in this study.  Methods included DNA isolation from fresh leaf, PCR, electrophoresis, sequencing, and data analysis using BLASTn program and MEGA software version 6.06.  The actin DNA fragment has been successfully amplified using the degenerate actin primer and then the actin primer specific for E. floribundus with the following sequences: 5?-CCAAATCATGTTTGAGACCT-3? (forward) and 5?- AACACGTTAATTCCTGCTC-3? (reverse) have been designed.  The primer pairs can be used to amplify the actin gene of E. floribundus.Keywords: actin, degenerate primer, Elaeocarpus floribundus, PCR, tuntun angin.
MORPHOLOGY, PHYSIOLOGY AND MOLECULAR CHARACTERISTICS OF OIL PALM (ELAEIS GUINEENSIS JACQ.) ENDOPHYTIC BACILLUS SP. Puspita, Fifi; Hadiwiyono, Hadiwiyono; Poromarto, Susilo Hambeg; Roslim, Dewi Indriyani
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2017.v05.i01.p07

Abstract

Endophytic bacteria are the bacteria that live in plant tissues. In oil palm tissue there are many types of endophytic bacteria and have a role that can be beneficial for the plant, one of them is endophytic Bacillus sp. The aim of these research was to obtain morphology, physiology and molecular characteristics of endophytic Bacillus sp. originating from oil palm tissue. Sampling was done by random simple sampling method. Isolation of bacteria was performed on plant tissues such as roots, midribs, stems and leaves of oil palm plants. The results of morphological characterization such as colony color, colony shape and colony edge show similarity in each isolate but there are differences in the surface morphology of the colony, where there are 6 isolates with convex surface and 6 isolates with flat shape. Physiological test results such as catalase test, oxidase test, starch hydrolysis test, motility test and temperature effect test on bacterial growth showed positive results in each isolate. Molecular characterization using 16S rRNA primers based on BLASTn shows that all isolates tested have similarities with Bacillus sp. Based on the phylogenetic tree it was found that the endophytic bacteria of Ba-B2 isolates were associated with Bacillus flexus with 100% consistency index grouped at a distance of 0.03 and Ba-P2 isolates were related to Bacillus substilis at a distance of 0.01 with an 89% consistency index.