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Journal : Jurnal Akuakultur Indonesia

THE EFFECTIVENESS OF HONEY SUPPLEMENTATION IN FEED FOR IMPROVING GOLDFISH FINGERLING CARASSIUS AURATUS IMMUNE SYSTEM AGAINST AEROMONAS HYDROPHILA BACTERIA ATTACK Rosidah, Rosidah; Subhan, Ujang; Mulyani, Yuniar; Dermawan, Rifai
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3658.789 KB) | DOI: 10.19027/jai.18.1.89-100

Abstract

ABSTRACT The attack of Aeromonas hydrophila bacteria can cause mortality in goldfish approximately 100%. The controlling of this bacterial attack can be done through increased fish immunity. Honey is one of the natural ingredients that increases body immune system. This study aimed to determine the effective dose of honey supplemented in feed to increase goldfish fingerling resistance for disease prevention. Fish used in this study were goldfish fingerlings with 3.5 g average weight. This study was done using experimental complete randomized design method with five treatments and three replications. Treatments given were honey supplementation in feed with 0 ml/kg (A) as control treatment, 150 ml/kg (B), 200 ml/kg (C), 250 ml/kg (D), and 300 ml/kg (E). The result showed that honey supplementation in feed was effective to improve goldfish fingerlings resistance against Aeromonas hydrophila bacterial attack. The supplementation of honey in feed with 200 ml/kg was the best treatment for inducing goldfish fingerlings against A. hydrophila. This was proven by the increased white blood cells (leucocytes) (27.84 ± 5.07%) followed with no apparent clinical symptoms after attacked by A.hydrophila, such as hemorrhage, necrosis, exophthalmia or dropsy, besides showing the highest survival rate with 73.33 ± 11.5%. Keywords : Aeromonas hydophila, goldfish, honey, leucocyte, resistance ABSTRAK Serangan bakteri Aeromonas hydrophila dapat menyebabkan kematian ikan mas koki hingga mencapai 100%. Penanggulangan serangan bakteri tersebut dapat dilakukan dengan meningkatkan ketahanan tubuh (imun) ikan.  Madu merupakan salah satu bahan alami yang dapat meningkatkan ketahanan tubuh. Penelitian ini bertujuan untuk menentukan dosis efektif penambahan madu pada pakan untuk meningkatkan daya tahan tubuh benih ikan mas koki dalam upaya pencegahan penyakit aeromonasis. Benih yang digunakan adalah benih ikan mas koki berukuran 3.5 gram. Metode penelitian yang digunakan adalah rancangan acak lengkap (RAL) dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah penambahan madu pada pakan dengan dosis 0 mL/kg (A) sebagai kontrol, 150 mL/kg (B), 200 mL/kg (C), 250 mL/kg (D), 300 mL/kg (E). Hasil penelitian menunjukkan bahwa penambahan madu ke dalam pakan efektif dalam meningkatkan ketahanan tubuh ikan mas koki terhadap serangan Aeromonas hydrophila. Dosis 200 ml/kg pakan memberikan hasil terbaik dalam meningkatkan ketahanan tubuh ikan mas koki terhadap serangan A. hydrophila terlihat dari peningkatan jumlah sel darah putih terbesar (27.84 ± 5.07%), tidak nampak adanya gejala klinis ikan terserang A. hydrophila seperti hemoragi, necrosis, exophthalmia maupun dropsy dan menghasilkan kelangsungan hidup benih ikan mas koki tertinggi yaitu sebesar 73.33 ± 11.5%. Kata kunci: Aeromonas hydrophila, ketahanan tubuh, ikan mas koki, madu, sel darah putih. 
THE EFFECTIVITY OF PANDANUS LEAF EXTRACT FOR THE TREATMENT OF SANGKURIANG CATFISH JUVENILE CLARIAS GARIEPINUS INFECTED BY AEROMONAS HYDROPHILA Sopiah, Siti; Rosidah, Rosidah; Lili, Walim; Iskandar, Iskandar; Suryadi, Ibnu Bangkit
Jurnal Akuakultur Indonesia Vol. 17 No. 2 (2018): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3486.475 KB) | DOI: 10.19027/jai.17.2.120-129

Abstract

ABSTRACTThe aim of this study was to determine the effective concentration of pandanus Pandanus amaryllifolius leaf extract for the treatment of sangkuriang catfish juvenile infected by Aeromonas hydrophila. The method used in this study was an experimental method with completely randomized design consisted of five treatments and three replications. The treatments were A (control), B (400 mg/L), C (600 mg/L), D (800 mg/L), and E (1000 mg/L) trough immersion for 24 hours through immersion for 24 hours.  The fish has infected with A. hydrophila bacteria as much as 20 mL of NaCl/L in 10 L of water medium with a density of 108 CFU/mL through immersion. The observed parameters were clinical symptoms, recovery process, survival rate, and water quality. The results showed that pandanus leaf extract at a concentration of 800 mg/L for 24 hours was effective for treating sangkuriang catfish juvenile that infected by A. hydrophila with a survival rate of 86.67%. Based on the regression analysis, it discovered that the optimum concentration of pandanus leaf extract through immersion for 24 hours was 774.39 mg/L and the predicted survival was 92.16%.  Keywords: Aeromonas hydrophila, catfish fingerlings, effective concentration, infection, pandanus leaf extract   ABSTRAKPenelitian ini bertujuan untuk menentukan konsentrasi yang efektif dari ekstrak daun pandan  wangi Pandanus amaryllifolius untuk pengobatan benih lele sangkuriang Clarias gariepinus yang terinfeksi bakteri A. hydrophila. Metode yang digunakan dalam penelitian ini adalah metode eksperimental rancangan acak lengkap dengan lima perlakuan dan tiga ulangan. Perlakuan penelitian adalah A (kontrol), B (400 mg/L), C (600 mg/L), D (800 mg/L), dan E (1000 mg/L). Ikan uji diinfeksi bakteri A. hydrophila sebanyak 20 mL NaCl/L dalam 10 L media air dengan kepadatan 108 CFU/mL melalui perendaman selama 24 jam. Parameter yang diamati meliputi gejala klinis, waktu pulih, kelangsungan hidup, dan kualitas air. Hasil penelitian menunjukkan bahwa penggunaan ekstrak daun pandan wangi pada konsentrasi 800 mg/L selama 24 jam efektif untuk pengobatan benih lele sangkuriang yang terinfeksi bakteri A. hydrophila dengan tingkat kelangsungan hidup sebesar 86,67%. Berdasarkan analisis regresi diketahui bahwa konsentrasi optimum penggunaan ekstrak daun pandan wangi melalui perendaman selama 24 jam yaitu 774,39 mg/L dan prediksi kelangsungan hidup ikan adalah 92,16%. Kata kunci : Aeromonas hydrophila, benih lele sangkuriang, ekstrak daun pandan, infeksi, efektifitas konsentrasi