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HUBUNGAN POLIMORFISME GEN TLR 9 (RS5743836) DAN TLR 2 (RS3804099 DAN RS3804100) DENGAN PEMBENTUKAN ANTI-HBS PADA ANAK PASCAVAKSINASI HEPATITIS B Rosalina, Ina
Indonesian Journal of Applied Sciences Vol 2, No 3 (2012)
Publisher : Universitas Padjadjaran

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Abstract

Current hepatitis B remains a worldwide health problem because it can cause chronic liver disease to hepatocellular carcinoma. One of the most important prevention is to vaccinate infants according to program. However, approximately 5-10% of healthy people fail to produce protective antibodies to the hepatitis B pasca vaccination, mean anti-HBs titers obtained <10 mIU/mL, are vulnerable to viral hepatitis B. One of the contributing factors is the disruption toll like receptor. This study aims to determine the Relation of polymorphisms gene toll like receptor 9 and 2 to the formation of anti-HB s in children pasca vaccination hepatitis B. At the time of blood sampling, the subjects were in good health, birth weight> 2500 g, and had a hepatitis B vaccination according to the program. Crossectional study conducted during the period 2010 to 2012. Obtained consisted of 162 infant boys 82 (50.6%) boys, 80 (49.4%) girls, with an age range 6-13.2 months (median 11.3). Were recorded characteristics, age, vaccination schedule, weight, and physical examination prior to blood sampling. Examination of anti-HBs titers, and examination of TLR 9 by restriction enzymes BstNI and TLR 2 by using the enzyme MwoI and MspI, which previously carried out DNA isolation. Statistical analysis using chi square for genotype and to use statistical tests HAPSTAT haplotype. The results obtained by 15 people (9.4%) anti-HBs titers <10mIU/mL (<2:00 - 8.45mIU/mL as non-response, based on the number of non response is obtained prevalence 9.4% (95% CI: 9.86 to 13, 9%). group with anti-HBs levels <10-100 mIU / mL a number of 144people (90.6%) referred to as a response. Detection of polymorphisms TLR9 -1237 T / C (rs5743836 T / C) with the enzyme BstNi. Found 6 the response of people on the subject. Detection of TLR2 gene polymorphism (rs3804099) with an enzyme found in 20 restrictions MwoI the subject response, whereas with the enzyme MspI restriction on TLR2 gene (rs3804100) obtained 1 (100%) heterozygote mutation in subject responses. Of the three haplotype The visible presence of an effect on the formation of anti-HBs after hepatitis B vaccination.
The Efficacy of Fucoidan on Gastric Ulcer Juffrie, Mohammad; Rosalina, Ina; Damayanti, Wahyu; Djumhana, Ali; A, Ariani; Ahmad, Harjono
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.732 KB)

Abstract

Hyperacidity causes gastric injury, and in severe situations, ulcer could develop. The growth factors known asthe basic fibroblast growth factor (bFGF) and the epidermal growth factor (EGF) have been recognized to promoteulcer healing. Fucoidan is extracted from a brown seaweed of Okinawa called Mozuku or Cladosiphon okamuranus.Fucoidan is effective for the healing of gastric ulcers by inducing epithelial cells to produce growth factors. The aimof this study is to explore the efficacy of fucoidan in patient who suffered by gastric ulcer. A randomized control trialdouble blind was conducted to 33 eligible samples. By using four-blocks random samples were divided into fucoidanand placebo groups. 100 mg of fucoidan was given to the fucoidan group and 100 mg of glucose was given to theplacebo group. Due to ethical reasons, for both groups were given a proton pump inhibitor. There was no differencein the age category between the fucoidan group (mean: 46.23 ± 14.8 years) and the placebo group (mean: 46.18 ± 18.4years) (p: 0.28). There was also no difference in sex between the fucoidan group (female: 10/33; male 7/33) and theplacebo group (female: 7/33; male: 9/33); p: 0.38. According to the SAKITA and MIWA criterias 32 patients fulfilledA1 which indicate active severe ulcer, and 1 patient fulfilled A2 which indicate active moderate ulcer. Most of theulcers were gastric ulcer. There was a significant improvement of the grade of ulcer in fucoidan group (94%) (16/17)compared to placebo group (37.5%) (6/16,p: 0.005). There was a significant reduction of abdominal pain after 5 daysin the fucoidan group, compared to the placebo group (p: 0.04). Vomiting tends to decrease in day 6 of the fucoidangroup however its proportion is similar with that of the placebo group (p: 0.9). Fucoidan is effective for ulcer healingand reducing ulcer symptoms.Key words : fucoidan, gastric ulcer, anti-peptic activity
Perbandingan Kadar Seng Plasma pada Diare Akut Gizi Baik dan Gizi Kurang Anak Usia 6 bulan-2 tahun Surjani, Riwi Dwi; Rosalina, Ina; MS, Azhali
Sari Pediatri Vol 10, No 3 (2008)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp10.3.2008.196-200

Abstract

Latar belakang. Diare menjadi salah satu penyebab utama morbiditas dan mortalitas pada anak di bawah usia 5 tahun, dengan insidens tertinggi pada usia 6 bulan–2 tahun. Selama diare berlangsung terjadi penurunan kadar seng plasma. Kondisi ini makin diperberat jika sebelumnya telah terjadi deplesi seng plasma pada anak dengan status gizi kurang.Tujuan. Membandingkan kadar seng plasma pada anak diare akut gizi baik dan gizi kurang usia 6 bulan–2 tahun.Metode. Comparative study dengan rancangan penelitian cross sectional yang dilaksanakan di RS Hasan Sadikin, RS Ujung Berung, dan RS Cibabat sejak Oktober sampai Desember 2007. Setiap subjek penelitian diambil sampel darah untuk diperiksa kadar seng plasma, status gizi diperiksa dengan skor Z. Kadar seng plasma <84,5 μg/dL dikategorikan rendah dan ≥84,5 μg/dL normal. Analisis statistik dihitung dengan menggunakan uji t untuk mengetahui perbedaan kadar seng plasma rata-rata antara anak diare akut gizi baik dan kurang serta uji x2 untuk mengetahui perbedaan antara kadar seng plasma normal dan rendah pada anak diare akut gizi baik dan kurang.Hasil. Dari 60 subjek penelitian terdapat 30 anak gizi kurang dan 30 anak gizi baik yang menderita diare akut. Terdapat 24 anak gizi kurang dan tujuh anak gizi baik yang memiliki kadar seng plasma di bawah nilai normal. Kadar seng plasma rata-rata pada anak diare akut gizi baik adalah (104,38±19,31) μg/dL dan (69,20±15,77) μg/dL pada anak diare akut gizi kurang (p=0,000). Hasil uji x2 menunjukkan kadar seng plasma anak diare gizi kurang secara bermakna lebih rendah daripada kadar seng plasma anak diare akut gizi baik (x2=19,288; p=0,000).Kesimpulan. Kadar seng plasma pada anak gizi kurang usia 6 bulan–2 tahun yang menderita diare akut lebih rendah dibandingkan dengan anak gizi baik.
Perbandingan Efek Live dan Heat-killed Probiotic Terhadap Penyembuhan Diare Akut Nondisentri pada Anak Kusumajaya, Reby; Rosalina, Ina; Rahayuningsih, Sri Endah
Sari Pediatri Vol 10, No 5 (2009)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp10.5.2009.302-7

Abstract

Latar belakang. Diare akut masih menjadi penyebab morbiditas dan mortalitas, terutama di negara berkembang. Probiotik sebagai mikroorganisme hidup jika dikonsumsi dalam jumlah cukup akan memberikan manfaat terhadap tubuh pejamu. Namun masih terdapat masalah teknologi probiotik yang sulit diatasi, yaitu hilangnya dosis dan aktivitas yang tidak dapat diprediksi karena kualitas penyimpanan sediaan yang beragam. Heat-killed probiotic sudah mengalami tindalisasi menawarkan sediaan farmasi yang lebih stabil dengan aktivitas yang sama.Tujuan. Menilai efektifitas heat-killed probiotic dibandingkan dengan live probiotic terhadap anak yang menderita diare akut nondisentri.Metode. Uji klinis acak tersamar buta ganda terhadap pasien diare akut nondisentri usia 6-24 bulan dengan atau tanpa dehidrasi ringan-sedang di RS Hasan Sadikin, RSUD Ujung Berung dan RSUD Cibabat pada periode bulan April-Mei 2008. Setiap subjek mendapat satu bungkus berisi 3 x 1010 probiotic atau 107 live probiotic, kemudian dilanjutkan dengan interval 8 sampai 12 jam per hari selama lima hari. Durasi dan frekuensi defekasi dicatat secara kuantitatif. Analisis statistik dihitung dengan menggunakan uji t.Hasil. Didapatkan 52 anak (25 heat-killed probiotic, 27 live probiotic) masuk ke dalam penelitian. Rata-rata durasi diare menurun secara bermakna pada pasien yang diberi heat-killed probiotic (50,2 jam SB 28,6) dibandingkan dengan live probiotic (74,8 jam SB 28,8) (p=0,003). Rata-rata frekuensi defekasi juga lebih rendah secara bermakna pada kelompok heat-killed probiotic dibandingkan dengan kelompok live probiotic (8,3;SB 7,4 vs 10,7;SB6,4).Kesimpulan. Heat-killed probiotic lebih baik dalam mengurangi lama diare dan frekuensi defekasi daripada live probiotic pada anak pasien diare akut nondisentri.
Hubungan Dosis Kumulatif Prednison dan Gangguan Umur Tulang pada Sindrom Nefrotik Relaps Sering Firdaus, Budi; Rosalina, Ina; Sekarwana, Nanan
Sari Pediatri Vol 10, No 6 (2009)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.128 KB) | DOI: 10.14238/sp10.6.2009.357-61

Abstract

Latar belakang. Pemberian prednison jangka panjang selama pengobatan sindrom nefrotik (SN) mengganggu proses pertumbuhan, terutama pertumbuhan kartilago secara langsung dan gangguan faktor-faktor pertumbuhan (growth factors). Pada SN relaps sering, selalu diberikan prednison jangka panjang yang berulang sehingga dapat menyebabkan gangguan pertumbuhan yang dapat diketahui dari gangguan umur tulang.Tujuan. Untuk mengetahui hubungan antara dosis kumulatif prednison dan gangguan umur tulang pada anak pasien SN relaps sering.Metode. Penelitian menggunakan rancangan cross-sectional. Subjek penelitian adalah anak pasien SN relaps sering, berumur 1-14 tahun, yang berobat jalan di poliklinik Subbagian Nefrologi Bagian Ilmu Kesehatan Anak Rumah Sakit Hasan Sadikin Bandung mulai bulan April sampai dengan Juni 2008. Dilakukan anamnesis, pemeriksaan fisis, penghitungan dosis kumulatif prednison selama pengobatan, dan pemeriksaan umur tulang. Analisis regresi linier multipel digunakan untuk menilai dosis kumulatif prednison, umur awitan penyakit, lama pemberian prednison, dan jumlah relaps, merupakan faktor risiko yang dapat menyebabkan gangguan umur tulang. Dinyatakan bermakna bila p<0,05.Hasil. Didapatkan 23 anak dengan rata-rata dosis kumulatif prednison (9.677,8±5.016,8) mg, umur awitan (53,30±24,7) bulan, lama pemberian prednison (36,3±22,2) bulan, jumlah relaps (4,5±1,7) kali, dan selisih umur tulang adalah (35,52±21,2) bulan. Analisis regresi multipel dari faktor risiko menunjukkan hanya dosis kumulatif prednison yang menunjukkan hubungan yang bermakna (p<0,05) sedangkan umur awitan penyakit akan menunjukan hubungan yang bermakna (p<0,05) bila jumlah sampel minimal 33.Kesimpulan. Terdapat hubungan yang bermakna antara dosis kumulatif prednison dan gangguan umur tulang pada anak pasien SN relaps sering.
Hubungan Kadar Laktat Plasma dengan Derajat Disfungsi Organ Berdasarkan Skor PELOD pada Anak Sakit Kritis Dharma, Aedi Budi; Rosalina, Ina; Sekarwana, Nanan
Sari Pediatri Vol 10, No 4 (2008)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp10.4.2008.280-4

Abstract

Latar belakang. Peningkatan kadar laktat menunjukkan hipoksia jaringan dan bila berlangsung lama akan menyebabkan kematian sel dan disfungsi organ. Skor PELOD (pediatric logistic organ dysfunction) merupakan skor komposisi yang dapat digunakan untuk menilai derajat disfungsi organ dan prediksi kematian.Tujuan. Mengetahui hubungan kadar laktat plasma dengan derajat disfungsi organ berdasarkan skor PELOD pada anak sakit kritis.Metode. Penelitian observasional analitik dengan rancangan cross sectional di Bagian Ilmu Kesehatan Anak RS dr. Hasan Sadikin Bandung pada April-Mei 2008. Pasien anak sakit kritis usia 1 bulan sampai 14 tahun dipilih secara konsekutif. Untuk menentukan korelasi antara kadar laktat plasma dan derajat disfungsi organ dilakukan dengan Spearman rank correlation. Kadar laktat dikelompokkan menjadi 2 kelompok, yaitu kadar laktat <2 mmol/L dan kadar laktat ≥2 mmol/L. Perbandingan antara kelompok kadar laktat dan distribusi umur, skor PELOD, dan jumlah disfungsi organ dilakukan uji Mann-Whitney. Variabel hipoperfusi dilakukan dengan uji chi-square. Hubungan antar variabel dengan regresi logistik.Hasil. Didapatkan 45 subjek dengan umur rata-rata 48,7 bulan. Jenis kasus kegawatan terbanyak adalah kegawatan kardiovaskular. Kadar laktat rata-rata 3,45 mmol/L dan rata-rata mengalami 3 disfungsi organ. Terdapat hubungan positif yang bermakna antara kadar laktat plasma dan derajat disfungsi organ berdasarkan skor PELOD (rs=0,54 p=0,001), juga dengan jumlah organ yang mengalami disfungsi. Kadar laktat plasma ≥3,3 mmol/L berhubungan dengan keadaan hipoperfusi.Kesimpulan. Terdapat hubungan antara kadar laktat plasma dan derajat disfungsi organ berdasarkan skor PELOD
Konstipasi dan Faktor Risikonya pada Sindrom Down Rosalina, Ina; Hidayat, Sjarif
Sari Pediatri Vol 6, No 1 (2004)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.145 KB) | DOI: 10.14238/sp6.1.2004.10-5

Abstract

Konstipasi adalah keterlambatan atau kesulitan buang air besar yang terjadi 1 sampai 2kali per minggu atau lebih dari 3 hari secara berturut-turut. Angka kejadian konstipasipada anak bervariasi antara 2-20%, pada umumnya merupakan suatu gejala dari penyakit.Kegagalan dalam proses defekasi merupakan penyebab utama dari konstipasi dan hipotonimerupakan salah satu keadaan yang mengakibatkan terjadinya konstipasi tersebut.Sindrom Down merupakan kelainan kromosom yang dapat mengakibatkan terjadinyahipotoni pada seluruh sistem muskuloskeletal termasuk pada saluran cerna. Penelitianini bertujuan untuk mengetahui persentase sindrom Down yang menderita konstipasidan hubungan dengan faktor risikonya. Diagnosis sindrom Down ditegakkan berdasarkanpemeriksaan genetik di rumah sakit Hasan Sadikin, dibagi dalam tipe aberasi penuh dantipe mosaik. Pada subyek diberikan kuesioner terstruktur yang berisi pertanyaan yangberhubungan dengan konstipasi dan faktor risikonya, 50 kuesioner kembali ke peneliti.Subyek berusia 3 - 10 tahun ( 27 wanita dan 23 pria), konstipasi ditemukan pada 32%.Aberasi penuh lebih banyak yang menderita konstipasi (35%) dibanding dengan mosaik(25%). Encopresis ditemukan pada 30% pasien sedangkan soiling pada 26% pasien.Baik encopresis maupun soiling lebih banyak ditemukan pada tipe mosaik ( 58,5%;38%) dibanding aberasi penuh ( 26,7% ; 25%). Nyeri perut dan nyeri saat buang airbesar berhubungan dengan kejadian konstipasi (r:0,5, p:0,002 ; r:0,56, p:0,002). Aktifitasberlebihan dan soiling berhubungan dengan kejadian encopresis (r: 0.49, p:0,001; r:0,44,p:0,005). Gangguan saluran kemih dan pemakaian obat obatan berhubungan dengankejadian soiling (r: 0,38, p: 0,02; r:0,32, p:0,04). Kesimpulan, hampir setengah darisindrom Down mengalami konstipasi, encopresis dan soiling; sedangkan tidak jelas faktorrisiko mempengaruhi terjadinya konstipasi pada anak sindrom Down.
The Efficacy of Fucoidan on Gastric Ulcer Juffrie, Mohammad; Rosalina, Ina; Damayanti, Wahyu; Djumhana, Ali; Ariani, A.; Ahmad, Harjono
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.732 KB) | DOI: 10.22146/ijbiotech.7565

Abstract

Hyperacidity causes gastric injury, and in severe situations, ulcer could develop. The growth factors known asthe basic fibroblast growth factor (bFGF) and the epidermal growth factor (EGF) have been recognized to promoteulcer healing. Fucoidan is extracted from a brown seaweed of Okinawa called Mozuku or Cladosiphon okamuranus.Fucoidan is effective for the healing of gastric ulcers by inducing epithelial cells to produce growth factors. The aimof this study is to explore the efficacy of fucoidan in patient who suffered by gastric ulcer. A randomized control trialdouble blind was conducted to 33 eligible samples. By using four-blocks random samples were divided into fucoidanand placebo groups. 100 mg of fucoidan was given to the fucoidan group and 100 mg of glucose was given to theplacebo group. Due to ethical reasons, for both groups were given a proton pump inhibitor. There was no differencein the age category between the fucoidan group (mean: 46.23 ± 14.8 years) and the placebo group (mean: 46.18 ± 18.4years) (p: 0.28). There was also no difference in sex between the fucoidan group (female: 10/33; male 7/33) and theplacebo group (female: 7/33; male: 9/33); p: 0.38. According to the SAKITA and MIWA criterias 32 patients fulfilledA1 which indicate active severe ulcer, and 1 patient fulfilled A2 which indicate active moderate ulcer. Most of theulcers were gastric ulcer. There was a significant improvement of the grade of ulcer in fucoidan group (94%) (16/17)compared to placebo group (37.5%) (6/16,p: 0.005). There was a significant reduction of abdominal pain after 5 daysin the fucoidan group, compared to the placebo group (p: 0.04). Vomiting tends to decrease in day 6 of the fucoidangroup however its proportion is similar with that of the placebo group (p: 0.9). Fucoidan is effective for ulcer healingand reducing ulcer symptoms.Key words : fucoidan, gastric ulcer, anti-peptic activity
Profile and Behaviour of Mother as Factor of Increasing Diarrhea Incidence Children Under Five at Jatinangor District in 2013 Widhyasti, Rahayu; Arisanti, Nita; Rosalina, Ina
Althea Medical Journal Vol 3, No 4 (2016)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.765 KB)

Abstract

Background: In 2012, data from Health Center of Jatinangor District showed nearly 50% of diarrhea cases occured in children under five at Sayang Village and Cilayung Village. Diarrhea cannot be separated from several factors that influence it, thus to reduce the incidence rate of diarrhea needs description of factors that affect diarrhea in children so that prevention can be done maximally. Objective of this study was to describe profile and behavior of mother as one factor of increasing diarrhea incidence in children under five at Jatinangor.Methods: This study was descriptive with cross-sectional design conducted at Sayang Village and Cilayung Village in October–November 2013. The respondents of this study were 102 mothers who have infants aged 0–59 months thorough cluster sampling methods. Respondent will be interviewed and given validated questionnaire. Results: The result showed that 57.8% of mother aged 25–35 years, mostly graduated from junior high education (41.2%), and 81.4% as a housewife. Most of diarrhea occurred in children under 2 years and female. There were 73.5% mother who exclusively breastfed, 94.1% have good behavior of clean water usage, 61.8% with good hand washing behavior, and 60.8% respondents utilized healthy latrine.Conclusions: This study showed good results in behaviour of exclusive breastfeeding, behavior of clean water usage, behaviour of hand washing with clean and water flow, and behaviour of healthy latrine usage. Other factors might be contributed to increasing diarrhea incidence. [AMJ.2016;3(4):655–60] DOI: 10.15850/amj.v3n4.956
Prevalence and Risk Factors of Asthma in Children Aged 13-14 Years Rosalina, Ina; Kartasasmita, Cissy B; Soepriadi, Myrna
Paediatrica Indonesiana Vol 38 No 11-12 (1998): November 1998
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2405.592 KB) | DOI: 10.14238/pi38.11-12.1998.265-72

Abstract

To determine the asthma prevalence and the role of some risk factors of asthma in children aged 13-14 years in Bandung, a study was conducted from April to May 1996 using using the International Study of Asthma and Allergies in childhood (ISAAC) written questionnaire. The study was conducted in 12 Junior high schools (SMP), selected by multistage population random sampling from 6 district in municipality of Bandung. A total of 3118 questionnaires were collected. The cumulative prevalence of asthma was 6.4% and the 12 month prevalence was 2.6%. The prevalence was higher in male, with a ratio of male : female of 1.48:1. The prevalence of asthma increased significantly in children with history of atopy in the family (OR 6.1; p&lt;0.01); however no significant differences was found between children with one or both parents having history of atopy. The prevalence of asthma in smoking children, maternal and room mates had also increased significantly (OR = 2.1; 2.3; 2.6 and p=&lt;0.05;&lt;0.01;&lt;0.01 respectively). However there was no significant effect of paternal smoking (p=0.074). The use of mosquito spray in the bedroom and having pets showed no effect on the prevalence of asthma (p=0.86 and 0.56 respectively).