Mohammad Saifur Rohman
Laboratorium Biokimia Fakultas Kedokteran Universitas Brawijaya

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Indonesian Doctors Understanding on Hypertension and its Problems in Daily Practice

Journal of the Indonesian Medical Association Vol. 61 No. 2 February 2011
Publisher : Journal of the Indonesian Medical Association

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Abstract

Morbidity and mortality rate of hypertension related disease remain relatively high. However, no survey had been performed in Indonesia up to now regarding the knowledge of hypertension among practitioners, how they choose antihypertensive drugs, the problems and complications due to hypertension faced in daily practice. This study aimed to collect data regarding the knowledge and its application among annual scientific meeting of Indonesian Society of Hypertension (InaSH) participants about hypertension, hypertension management and its problems. This study performed by collecting and analyzing quistionnaires during annual scientific meeting of InaSH 2007 to 2009. The data presented as percentage of the lowest and the highest values. Result of survey analysis concluded that there was a significant increases in participant interest during the first three years of InaSH, with the number of general practioner as large as specialist. The scientific meeting was the major source of information for respondents. As many as 74.5% of respondents measure blood pressure properly. The knowledge about hypertension therapy is quite well, as more than 50% of respondents have addressed all question correctly. The most common target organ damage observed was brain, heart and kidney, whereas the most common cause of uncontrolled blood pressure was irregular medication.Keywords: Knowledge, Problem, hypertension

Left Ventricular Hypertrophy is Associated with Increased Ostial Pulmonary Vein Diameter

Jurnal Kardiologi Indonesia VOL. 28 NO. 6 Desember 2007
Publisher : Indonesian Heart Association

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Abstract

Background: Hypertensive heart disease, characterized with left ventricular hypertrophy (LVH), which is usually accompanied with left ventricular dysfunction, is prevalent in the community and associated with increased of ostial pulmonary vein diameter. We investigate pulmonary vein diameter in its relationship to left ventricular hypertrophy.Methods and Results: Ostial pulmonary veins divided into four parts, left superior (LS), right superior (RS), left inferior (LI),and right inferior (RI), were assessed by spiral multisliced computed tomography (MSCT) in 46 sinus rhythm subjects. LVH was analyzed by measuring the thickness of interventricular septum by MSCT. There were 23 patients with LVH, and the rest were normal. According to Pearson correlation analysis, we found a significant positive correlation between the degree of LVHand ostial diameter of RS, RI, and LS (p<0.05).Conclusion: Left ventricular hypertrophy is associated with right superior, right inferior and left superior pulmonary veins dilatation.

The Effect Of Red Rosella Tea (Hibiscus sabdariffa Linn) on the Inhibition of NF-κB Activation, TNF-α and ICAM-1- Protein Expressions Following Treatment with Ox-LDL in HUVECs

Jurnal Kardiologi Indonesia Vol.28 No.2 Mar 2007
Publisher : Indonesian Heart Association

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Abstract

Background. Atherosclerosis disease has been a global threat. Ox-LDL was one of major source of atherogenesis process, through the ROS formation which would then activate NF-KB and increase the expression of TNF-α and ICAM-1, leading to the occurrence of inflammation. Recent studies indicate that red Rosella tea is a tropical plant containing antioxidant (protocatechuic acid and antocyanin) and ascorbate acid. This tea is widely distributed in Indonesia. The mechanism of action of this tea on the inhibition of atherogenesis has not been largely known. This study aim to investigate the effect and mechanism of the red Rosella tea on the expression of NFKB, TNF-α and ICAM-1.Methods and results. Cultured endothelial cell, HUVECs (Human Umbilical Vein Endothelial), is used for a model of endothelial cells. There are 5 groups: 1) HUVECs culture without Ox-LDL as negative control; 2)HUVECs culture with Ox-LDL as positive control; and 3 groups given various dosages of the red Rosella tea (0.001 mg/ml, 0.005 mg/ml and 0.01 mg/ml) and Ox-LDL. OxLDL (40 mg/ml) is added on HUVECs to stimulate responds of endothelial cells mimicking dyslipidemia condition. The Red Rosella tea were given 2 h before Ox-LDL treatment. The NF-KB activaton,the protein expressions of TNF-α and ICAM-1 are detected by immunohistochemistry. Treatment with red rosella tea on Ox-LDL-treated HUVECs resulted inprevention of NF-KB activation which in turn inhibited the increased of TNF-α and ICAM-1 protein expressions. Spearman‘s correlation analysis showed that there is negative correlations between dosages of red tea with NF-KB activation, TNF-α and ICAM-1 protein expressions (p=0.01, Correlation Coeff= -1).Conclusions. This study indicates the benefit of red Rosella tea on the prevention and medication for atherosclerosis disease.

ANALISIS URUTAN NUKLEOTIDA DAN EKSPRESI DARI MUSCLE A-KINASE ANCHORING PROTEIN (mAKAP) MENUNJUKKAN KEMUNGKINAN FUNGSI mAKAP PADA DIFERENSIASI KARDIMIOSIT: PERBANDINGAN EKSPRESI mAKAP PADA JANTUNG MDX DAN KONTROL

Jurnal Kedokteran Brawijaya Vol 20, No 1 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

Muscle A-Kinase Anchoring Protein (mAKAP) is an A-kinase anchoring protein (AKAP) which targets cAMP-dependent protein kinase(PKA) to the nuclear envelope. mAKAP not only binds to PKA, but also to the ryanodine receptor (RyR2) and the rolipram-inhibitedcAMP-specific phosphodiesterase (PDE4D3). Amino acid sequence analysis revealed that mAKAP possesses LXCXE andFYDYSYL, the consensus-binding domain of pRB and CBP/p300, respectively. pRB and CBP/p300 are known as a key componentsin cardiomyocyte differentiation processes. Northern blot analysis revealed that mAKAP was expressed in a 13 day old rat heart andits expression increased by 15 days of age when cardiomyocytes reveal a terminal differentiation phenotype. In culturedcardiomyocytes mAKAP was expressed in differentiated but not undifferentiated. Accordingly, mAKAP may play a role in the terminaldifferentiation process through pRB-CBP/p300 functions. Furthermore, we observed mAKAP expression in old mdx heart, a mousemodel of Duchenne muscular dystrophy, compared to control mice. In the control heart, mAKAP transcripts were detected at 6-, 20-,64- and 76-weeks of age. However, mAKAP expression significantly appeared only in 64- and 76-week old mdx hearts. DelayedmAKAP expression in mdx may contribute to impaired function of pRB, CBP/p300, cAMP and Ca2+ complex.Key words: mAKAP, domain architecture, terminal differentiation, pRB, CBP/p300, mdx.

PENGHAMBATAN AKTIFASI NFκ κκ κB OLEH CAPE (CAFFEIC ACID PHENETHYL ESTER), KOMPONEN AKTIF MADU LEBAH (HONEYBEE HIVES),PADA HUVEC’S (HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS) YANG DIPAPAR LDL TEROKSIDASI

Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

The high prevalence of cardiovascular morbidity and  mortality caused by complication of atherosclerosisneeds an optimal effort to prevent the atherosclerosis progression and complication. Atherogenesis is a chronicinflammatory process which could be prevented via the inhibitionof NFκB, a known key transcription factor involved in inflammatory process. An active component of honeybee hives, CAPE (Caffeic Acid Phenethyl Ester), is believed to inhibit inflammatory process via the inhibition of NFκB activation. However, it remains uncertain whetherCAPE inhibits NFκB activation in endothelial cells. This study therefore was aimed to examine the molecular mechanism of CAPE mediated NFκB inactivation in OxLDL-treated HUVEC’s. Immunohistochemistry using p50 antibody was applied to detectthe migration of NFκB (p50-p65 complex) from inactive form in cytoplasmto active form in nucleus.

PENGHAMBATAN AKTIFASI NFκ κκ κB OLEH CAPE (CAFFEIC ACID PHENETHYL ESTER), KOMPONEN AKTIF MADU LEBAH (HONEYBEE HIVES),PADA HUVEC’S (HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS) YANG DIPAPAR LDL TEROKSIDASI

Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

The high prevalence of cardiovascular morbidity and  mortality caused by complication of atherosclerosisneeds an optimal effort to prevent the atherosclerosis progression and complication. Atherogenesis is a chronicinflammatory process which could be prevented via the inhibitionof NFκB, a known key transcription factor involved in inflammatory process. An active component of honeybee hives, CAPE (Caffeic Acid Phenethyl Ester), is believed to inhibit inflammatory process via the inhibition of NFκB activation. However, it remains uncertain whetherCAPE inhibits NFκB activation in endothelial cells. This study therefore was aimed to examine the molecular mechanism of CAPE mediated NFκB inactivation in OxLDL-treated HUVEC’s. Immunohistochemistry using p50 antibody was applied to detectthe migration of NFκB (p50-p65 complex) from inactive form in cytoplasmto active form in nucleus.

Pengaruh Pemberian Ekstrak Buah Mengkudu (Morinda citrifolia L.) Terhadap Aktivasi NF-ĸβ dan Ekspresi Protein (TNF-α, ICAM-1) pada Kultur Sel Endotel (HUVECs) Dipapar Ox-LDL

The Journal of Experimental Life Science Vol 1, No 1 (2011)
Publisher : Graduate School, University of Brawijaya

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Abstract

Penelitian ini bertujuan mengetahui pengaruh dan mekanisme kerja mengkudu (Morinda citrifolia) terhadap aktivasi NF-κβ, ekspresi protein TNF-α dan ICAM-1 dalam menghambat proses aterosklerosis. Penelitian dilakukan secara in vitro menggunakan kultur sel endotel vena umbilikalis manusia (HUVECs). Dibuat dua kelompok kontrol pada kultur sel endotel yaitu kontrol negatif tanpa perlakuan, kelompok yang dipapar Ox-LDL 40 μg ml-1, Kelompok perlakuan yaitu kultur sel endotel dengan pemberian dosis ekstrak mengkudu 2,5 μg ml-1, 5 μg ml-1 dan 10 μg ml-1 selama dua jam. Masing-masing sistem kultur dipapar Ox-LDL 40 μg ml-1. Pemaparan Ox-LDL dilakukan selama 30 menit untuk mengetahui aktivasi NF-κβ dan 24 jam untuk mengetahui ekspresi protein TNF-α dan ICAM-1. Pengukuran aktivasi NF-κβ, ekspresi protein TNF-α dan ICAM-1 menggunakan imunohistokimia. Hasil penelitian menunjukkan, ekstrak mengkudu dengan dosis 2,5 μg ml-1, 5 μg ml-1 dan 10 μg ml-1 dapat menghambat aktivasi NF-κβ, ekspresi protein TNF-α dan ICAM-1 pada kultur sel endotel manusia (HUVECs) dipapar Ox-LDL 40 μg ml-1 sebagai agen inflamasi yang dapat menimbulkan aterosklerosis. Melalui analisis ANOVA (p<0,01) diketahui terdapat hubungan negatif pada perlakuan antar dosis ekstrak mengkudu dalam menghambat aktivasi NF-κβ, ekspresi protein TNF-α dan ICAM-1 menggunakan analisis Spearman’s (p<0,01).Kata kunci: aterosklerosis, ICAM-1, mengkudu (Morinda citrifolia L.), NF-κβ, Ox-LDL, TNF-α

ANALISIS URUTAN NUKLEOTIDA DAN EKSPRESI DARI MUSCLE A-KINASE ANCHORING PROTEIN (mAKAP) MENUNJUKKAN KEMUNGKINAN FUNGSI mAKAP PADA DIFERENSIASI KARDIMIOSIT: PERBANDINGAN EKSPRESI mAKAP PADA JANTUNG MDX DAN KONTROL

Jurnal Kedokteran Brawijaya Vol 20, No 1 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Original Source | Check in Google Scholar

Abstract

Muscle A-Kinase Anchoring Protein (mAKAP) is an A-kinase anchoring protein (AKAP) which targets cAMP-dependent protein kinase(PKA) to the nuclear envelope. mAKAP not only binds to PKA, but also to the ryanodine receptor (RyR2) and the rolipram-inhibitedcAMP-specific phosphodiesterase (PDE4D3). Amino acid sequence analysis revealed that mAKAP possesses LXCXE andFYDYSYL, the consensus-binding domain of pRB and CBP/p300, respectively. pRB and CBP/p300 are known as a key componentsin cardiomyocyte differentiation processes. Northern blot analysis revealed that mAKAP was expressed in a 13 day old rat heart andits expression increased by 15 days of age when cardiomyocytes reveal a terminal differentiation phenotype. In culturedcardiomyocytes mAKAP was expressed in differentiated but not undifferentiated. Accordingly, mAKAP may play a role in the terminaldifferentiation process through pRB-CBP/p300 functions. Furthermore, we observed mAKAP expression in old mdx heart, a mousemodel of Duchenne muscular dystrophy, compared to control mice. In the control heart, mAKAP transcripts were detected at 6-, 20-,64- and 76-weeks of age. However, mAKAP expression significantly appeared only in 64- and 76-week old mdx hearts. DelayedmAKAP expression in mdx may contribute to impaired function of pRB, CBP/p300, cAMP and Ca2+ complex.Key words: mAKAP, domain architecture, terminal differentiation, pRB, CBP/p300, mdx.

Development of an Experimental Model of Metabolic Syndrome in Sprague Dawley Rat

Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

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Abstract

The study was designed to establish an experimental models of metabolic syndrome that adequately mimic metabolic syndrome criteria as determined by NCEP ATP III.Eighteen Male Sprague dawley rats, 2 - 3 months old were used in the study. Combination of high fat and high sucrose (HFHS) diet for eight weeks and streptozotocin (STZ) injection in the second week was administered to induce metabolic syndrome. The body weight and biochemical parameters (blood glucose, triglyceride, HDL-cholesterol) were measured every 2 weeks. The rats with blood glucose (>126mg/dL), triglyceride (TG) (>150mg/dL), high systolic blood pressure (≥140 mmHg), and reduced HDL levels (<40 mg/dL) confirmed presence NCEP-ATP III criteria of metabolic syndrome. The adiponectin level was analyzed by ELISA methods.Fasting blood glucose, triglyceride, and systolic blood pressure increased significantly (p<0.05) in HFHS group compared to that of NC group. Moreover, after 8 week a significant lower HDL level was observed in HFHS group compared to that of NC group. In addition, HFHS group showed a significantly lower adiponectin level compared to that of NC group.The combination of low doses of STZ (30mg/kg) and HFHS administration for 8 weeks could induce metabolic syndrome mimicking human criteria of metabolic syndrome.

Physalis minima Leaves Extract Induces Re-Endothelialization in Deoxycorticosterone Acetate-Salt-Induced Endothelial Dysfunction in Rats

Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

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Abstract

The administration of deoxy-corticosterone acetate (DOCA)-salt can induce oxidative stress leading to decrease the bioavailability of nitric oxide (NO), increase senescence of circulating endothelial progenitor cells (EPCs), thus contributing to endothelial dysfunction. This study was aimed to investigate the effects of Physalis minima L. leaves extract on serum NO levels, circulating EPCs number, and histopathology of tail artery endothelial cells in DOCA-salt-induced endothelial dysfunction in rats. Twenty-five male Wistar rats were randomly divided into five groups: rats without any treatment (normal), rats treated with DOCA (10 mg/kgBW s.c. twice weekly) and given 0.9% NaCl to drink ad libitum for 6 weeks, and DOCA-salt-induced rats orally supplemented with P. minima leaves extract at doses of 500, 1500, or 2500 mg/kgBW for 4 weeks. Serum NO levels were measured by colorimetry. The number of circulating EPCs (CD34+/CD133+ cells) was determined by flow cytometry. The tail artery sections were histologically processed with hematoxylin-eosin staining. DOCA-salt-induced rats showed significantly (p<0.05) decrease in serum NO levels and circulating EPCs number compared to the normal. There was also more detached tail artery endothelial cells in DOCA-salt-induced rats. P. minima leaves extract at a dose of 500 mg/kgBW significantly (p<0.05) increased serum NO level and circulating EPCs number, and also induced an optimal re-endothelialization in DOCA-salt-induced rats. P. minima leave extract dose-dependently increases NO bioavailability contributing to enhanced EPCs mobilization, thereby promoting re-endothelialization in DOCA-salt-induced endothelial dysfunction in rats.