Articles

Viability of Frozen-Thawed Epididymal Sperm of Garut Ram

HAYATI Journal of Biosciences Vol 12, No 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Sperm collected from cauda epididymis is a source of male gametes. The purposes of this study was to evaluate an quality of frozen-thawed sperm of garut ram which was collected from cauda epididymis and cryopreserved with modified Tris extender, i.e: Tris extender (control, KT), Tris extender + 60 mM lactose (LS), and Tris extender + 60 mM lactose + 0.05% glutathione (GL). Quality of collected sperm including concentration, motility, live sperm, abnormality, cytoplasmic droplet, intact acrosomal cap (IAC), and intact plasma membrane (IPM) were evaluated. Results showed that mean of sperm concentration, percentages of motility, live sperm, abnormality, cytoplasmic droplet, IAC, and IPM of fresh epididymal sperm were 13,993.33 million/ml, 70.83, 82.83, 10.83, 8.5, 85.83, and 81.33%, respectively. Sperm quality after equilibration for LS and GL were significantly (P

Quality of Epididymal and Ejaculated Sperms of Spotted Buffalo in Dextrose Supplemented Extender

HAYATI Journal of Biosciences Vol 17, No 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Quality of epididymal and ejaculated sperms of spotted buffalo in three different extenders (i.e. Andromed, Andromed supplemented with 0.2% dextrose, and Andromed supplemented with 0.4% dextrose) was studied.  The results showed that there was no significantly different (P > 0.05) quality of ejaculated and epididymal sperms.  The motility percentage of post thawing epididymal and ejaculated sperms was 41-46% and 41-45%, respectively.  And percentage of cytoplasmic membrane integrity of the post thawing epididymal and ejaculated sperms was 66-67% and 47-54% respectively. There was also no significant different (P > 0.05) in the percentage of cytoplasmic membrane integrity between the extenders of both ejaculated and epididymal sperms. This result suggested that epididymal sperms were reliable for artificial insemination as good as ejaculated sperms.

Peningkatan Kualitas Spermatozoa Epididimis Kerbau Belang yang Dikriopreservasi dengan Beberapa Konsentrasi Sukrosa (THE QUALITY ENHANCEMENT OF EPIDIDYMAL SPERMATOZOA OF SPOTTED BUFFALO CRYOPRESERVING WITH VARIOUS SUCROSE CONCENTRATIONS)

Jurnal Veteriner Vol 8, No 4 (2007)
Publisher : Jurnal Veteriner

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Abstract

Penelitian dilakukan untuk mengetahui kualitas spermatozoa epididimis kerbau belang selama proses pembekuan (kriopreservasi). Bahan pengencer yang digunakan adalah AndroMed® sebagai kontrol (AM) serta kombinasi AndroMed® dengan 0,2% (S0,2) dan 0,4% sukrosa (S0,4). Hasil penelitian menunjukkan bahwa persentase motilitas spermatozoa epididimis kerbau belang setelah thawing dalam bahan pengencer AM (41%), nyata (P<0,05) lebih rendah dibandingkan dengan S0,2 (46%) dan S0,4 (46%). Demikian pula halnya dengan persentase hidup spermatozoa setelah thawing dalam bahan pengencer AM, S0,2, dan S0,4 secara berturut-turut adalah 52,2; 59,8 dan 60,8% (P<0,05). Sementara itu, tidak terdapat perbedaan nyata (P>0,05) pada persentase membran plasma utuh (MPU) setelah thawing dalam ketiga bahan pengencer. Persentase MPU setelah thawing perlakuan AM, S0,2, dan S0,4 adalah masing-masing 68; 68,8 dan 66,8%. Dapat disimpulkan bahwa penambahan sukrosa sebagai krioprotektan eksternal ke dalam bahan pengencer AndroMed® dapat meningkatkan kualitas spermatozoa epididimis kerbau belang setelah thawing.

PERANAN BETA-KAROTEN DALAM MEMPERTAHANKAN DAYA HIDUP SPERMATOZOA SEMEN CAIR DOMBA GARUT ROLE OF BETA-CAROTENE INI MAINTAINING SPERM LIVABILITY OF CHILLED-SEMEN OF GARUT RAM

Jurnal Veteriner Vol 7, No 4 (2006)
Publisher : Jurnal Veteriner

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Abstract

Tujuan penelitian ini adalah untuk mengevaluasi kualitas semen cair domba garut yang diencerkan dengan larutan pengencer tris dengan penambahan berbagai konsentrasi beta-karoten. Semen dikoleksi dengan vagina buatan satu kali dalam satu minggu dari empat pejantan domba garut dewasa kelamin. Segera setelah dievaluasi, semen segar dibagi ke dalam tiga buah tabung reaksi dan masing-masing diencerkan dengan pengencer tris (kontrol), pengencer tris + 0,0125% beta-karoten

Laktosa Mempertahankan Daya Hidup Spermatozoa Kambing Peranakan Etawah yang Dipreservasi dengan Plasma Semen Domba Priangan

Jurnal Veteriner Vol 10, No 3 (2009)
Publisher : Jurnal Veteriner

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Abstract

The purpose of this research was to examine the effect of lactose in Tris extender on theviability of Etawah crossbreed goat spermatozoa which is preserved in the plasma using Prianganram seminal plasma at 3–5oC. Semen was collected using artificial vagina once a week. Freshsemen was divided into four tubes, and centrifuged at 3,000 RPM for 30 min. Supernatant wasremoved and replaced which an equal volume with Priangan ram seminal plasma. Semen in firsttube was diluted with Tris extender-20% egg yolk without lactose (control). Semen in second,third, and fourth tubes were diluted with Tris extender-20% egg yolk and 0.3% (0.3 g per 100 mlextender) (L0.3), 0.6% (L0.6), and 1.2% (L1.2) lactose, respectively. Diluted-semen were stored inrefrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS),live spermatozoa (LS), and intact plasma membrane (IPM) were evaluated daily during storage at3–5oC for four days. The data were analyzed by usi analysis of variance (ANOVA). Means werecompared significant difference test at 0.05 significant level. Results of this study showed thatmean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS,spermatozoa abnormal, and IPM of Etawah crossbreed goat fresh semen were 0.68 ml, cream,thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12%, and 84%, respectively. Addition oflactose in Tris extender could maintain the quality of semen during preservation. At day-5 ofstorage, the percentages of MS, LS, and IPM for treatment L0.6 (40, 55.6, and 53.8%) weresignificantly (P<0.05) higher than treatment L1.2 (36, 50.2, and 51.8%), treatment L0.3 (34, 51.2,and 48.4%), and control (29, 41.4, and 41.8%). In conclusion, addition of 0.6% lactose in Trisextender is the best dose in maintaining the quality of Etawah crossbreed goat spermatozoa, andcould be maintaining percentage of motile spermatozoa 40% for four days after preserved at 3–5oC.

Maltosa Mempertahankan Viabilitas Spermatozoa Epididimis Kerbau Belang yang Disimpan dalam Bentuk Cair

Jurnal Veteriner Vol 11, No 2 (2010)
Publisher : Jurnal Veteriner

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Abstract

A study to determine the effect of maltose supplement in andromed based semen extender on theviability of buffalo’s epididymal spermatozoa following 12 and 24 hour storage as liquid semen. Epididymalspermatozoa were collected by combined methods of slicing, flushing and tissue pressure. The spermatozoawas then diluted with three different extenders, i.e Andromed only (A) as control, Andromed + 0.2% w/vMaltose (M1) and Andromed + 0.4% w/v Maltose (M2) as treatments. The percentages of progressivelymotil spermatozoa in the liquid semen in the following treatment of A, M1 and M2 were 48.33%; 53.33%and 55% respectively (after 12h of storage), and 45%; 46.67% dan 45% respectively (after 24h of storage).Besides, the percentage of live spermatozoa in A, M1 and M2 after 12h of storage were 70.33%; 72 % and72.33 % and after 24h of storage were 66.33%; 70% and 70.67 %. In conclusion, the addition of 0.2% and0.4% w/v maltose into Andromed extender could maintain the life of the spotted buffalo epididymalspermatozoa after storage for 4°C up to 24 hours.

Penentuan Siklus Estrus Berdasarkan Gambaran Sitologi Vagina dan Profil Hormon pada Rusa Timor

Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Jurnal Veteriner

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Abstract

Determination of estrous cycle especially the length of estrous is an important role in improvingthe fertility and reproductive performance of farm animals. This study was aimed to observe thelength of estrous cycle and estrous period based on cytologic and estrogen (E2) and progesterone (P4)hormone profiles. Samples of vaginal smears were collected daily and stained with Giemsa. Bloodsamples were collected every two days using jugular vein implant catheter. The hormone profileswere analized by radio immuno assay. All data were analyzed descriptively. Results of this studyshowed that there were four lowest points percentage of parabasal epithelial cells with the averageof 14.25%, while the superficial epithelial cells showed four highest points (85.75%). The intervalsbetween lowest point of parabasal epithelial cell and the highest point of superficial cells were at 20,18, and 16 with the average of 18 days. This phenomenon showed a cyclical processed with almostthe same length of time of the actual an estrous cycle pattern in timor deer. The range of E2 concentration was 7.06 to 18.14 pg/ml and P4 concentration was 2.58 to 7.48 ng/ml. The intervaltime between the peak of E2 was 17 days which represented the estrous cycle of timor deer. It isconcluded that analysis of estrous cycle in timor deer can be detected by vaginal cytological andhormone analysis profiles.

Utilization of Epididymal Sperm in Reproductive Technology

WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 14, No 1 (2004): MARCH 2004
Publisher : Indonesian Center for Animal Research and Development

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Abstract

Cauda epididymal sperm could be used as an alternative of male gamete source for application in reproductive technology, because the sperm is motile and has ability for fertilizing oocyte. This sperm could be collected from life or death animal, then it might be processed for chilling or freezing. Epididymis from death animal could also be preserved at 5°C for several days before collecting and processing. Cauda epididymal sperm which has motility more than 40% can be applied for artificial insemination (AI) or in vitro fertilization (IVF) programs. Key words: Cauda epididymal spermatozoa, Al, IVF

PENGEMBANGAN LKS FISIKA BERBASIS TEORI KECERDASAN MAJEMUK (MULTIPLE INTELLIGENCE) MATERI ALAT OPTIK PADA KELAS VIII SMP NEGERI 01 MADIUN

Inovasi Pendidikan Fisika Vol 1, No 1 (2012)
Publisher : Inovasi Pendidikan Fisika

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Abstract

Pendekatan, strategi, metode, dan teknik pembelajaran pada hakikatnya dapat dibedakan tetapi tidak dapat dipisahkan. Keempat istilah tersebut merupakan satu kesatuan dalam pembelajaran. Pendekatan, strategi, metode, dan teknik pembelajaran yang akan dan/atau sedang digunakan dapat diketahui dari langkah-langkah pembelajaran yang telah tersusun dan/atau sedang terjadi. Pendekatan pembelajaran adalah cara umum dalam memandang pembelajaran. Sedangkan strategi pembelajaran adalah ilmu dan kiat di dalam memanfaatkan segala sumber belajar yang dimiliki dan/atau yang dapat dikerahkan untuk mencapai tujuan pembelajaran yang telah ditetapkan. Metode mengajar adalah berbagai cara kerja yang bersifat relatif umum yang sesuai untuk mencapai tujuan pembelajaran tertentu dan teknik pembelajaran adalah ragam khas penerapan suatu metode sesuai dengan latar penerapan tertentu. Teknik pembelajaran mengambarkan langkah-langkah penggunaan metode mengajar yang sifatnya lebih operasional. Faktor-faktor yang perlu diperhatikan dalam penentuan teknik pembelajaran di antaranya adalah kemampuan dan kebiasaan guru, ketersedian sarana dan waktu, serta kesiapan siswa. Faktor-faktor yang perlu dipertimbangkan dalam memilih strategi pembelajaran ialah tujuan pembelajaran, jenis dan tingkat kesulitan materi pelajaran, sarana, waktu yang tersedia, siswa, dan guru.

Kriopreservasi Semen Kambing Boer dengan Konsentrasi Pengencer Nira Aren dan Gliserol Berbeda

Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

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Abstract

ABSTRAK                                                                        Penelitian bertujuan untuk mengetahui efektivitas nira aren sebagai pengencer alternatif dalam proses pembekuan (kriopreservasi) semen kambing boer.Kriopreservasi semen kambing boer menggunakan pengencer tris-gliserol-kuning telur (P1 73-7-20%), nira aren-gliseol-kuning telur(masing-masing P2 74-6-20%, P3 73-7-20%, dan P4 72-8-20%) dan andromed (P5 tanpa mengandung kuning telur dan gliserol). Parameter evaluasi meliputi motilitas, viabilitas, dan membrane plasma utuh setelah pengenceran, ekuilibrasi dan thawing.  Evaluasi motilitas pasca thawing menunjukkan P5 52% berbeda nyata (P<0.05) dengan P1 42%, selanjutnya P5 dan P1 berbeda sangat nyata (P<0.05) dengan P2 8%, P3 6% dan P4 12%.  Viabilitas pasca thawing menunjukkan P5 65,4% tidak berbeda nyata (P>0,05) dengan P1 61,8%, akan tetapi P5 dan P1 berbeda sangat nyata (P<0.05) dengan P2 26,2%, P3 29,8%, dan P4 34%.  Membran plasma utuh (MPU) pasca thawing menunjukkan P5 66,2% tidak berbeda nyata (P>0,05) dengan P1 65,4%, akan tetapi keduanya berbeda sangat nyata (P<0.05) dengan P2 39%, P3 38%, dan P4 36,2%.  Disimpulkan kriopreservasi semen kambing boer dengan pengencer nira aren dan gliserol pada konsentrasi berbeda belum dapat dipergunakan sebagai sumber bibit berdasarkan standar nasional Indonesia.Kata Kunci : Kambing boer, semen, nira arenABSTRACTThe experiment was conducted to determine the effect of sugar palm juice as alternative extender for cryopreservation process of boer semen.Tris-glycerol-egg yolk (P1 73-7-20%), Sugar palm juice-glyserol-egg yolk (P2 74-6-20%, P373-7-20%, dan P4 72-8-20%), and andromed (P5) used as a extender  in the cryopreservation process of boer semen.  Sperm motility (%), live sperm (%) and sperm membrane integrity (%) were recorded after diluted, equilibration and freeze-thawing.  Result of post thawing motility showed that P5 52% was significantly different (P <0.05) with P1 42%, then P5 and P1 were significantly different (P <0.05) with P2 8%, P3 6% and P4 12%. Viability after thawing showed P5 65.4% was not significantly different (P> 0.05) with P1 61.8%, but P5 and P1 significantly different (P <0.05) with P2 26.2%, P3 29.8 %, and P4 34%. Spermmembrane integrity post-thawing showed P5 66.2% was not significantly different (P> 0.05) with P1 65.4%, but both were very significantly different (P <0.05) with P2 39%, P3 38% and P4 36.2%. Conclusions, sugar palm juice-glycerol-egg yolk with differentconcentrationsineffectively as an alternative extenderin cryopreservation of boer semen.Keywords: boer goat, semen, sugar palm juice

Co-Authors ,, Sriwinar - Wasis . Herdis . Yulnawati Abrani Sulaiman Achmad Selamet Aku Agus Setiawan Ahadian, Edward Risky Ahadian, Edward Rizki Akbar Iskandar, Akbar AL-AZHAR AL-AZHAR Alam, Teuku Samsul Ali Nurrakhmad Siregar, Ali Nurrakhmad Anis Wahdi ARIEF BOEDIONO B Purwantara Bambang Purwantara Bintal Amin Budiansa, Akbar Budy Wiryawan Che Hassan Che Haron Darmawan, I Wayan Angga Dasrul Dasrul Demianus Ferdinand Souhoka Dessy Yoswaty Diana, Farah Dwi Sulistiowati, Dwi Dwitya, Yonadiah Elly Warni Fazrin, Khairul Febri Yulini, Febri Firdawaty Marasabessy, Firdawaty Hendra Saputra HERA MAHESHWARI Herdis - Herdis . herdis herdis Iin Solihin Insun Sangadji Irmawita Irmawita Isya, Mochammad Jaharah A. Ghani John Haluan KARTINI ERIANI M.R Toelihere Maman Surachman Michel Johan Matatula Misbah, Zulkarnain K Muhammad Gunawan Muhammad Iqbal Muhammad Riyadhi Muhammad Rusmin, Muhammad Muhammad Thahir Najmuddin MA, Najmuddin Nasrullah . P Situmorang Raden Iis Arifiantini Raja, Sivi Kurniati Ramadhani, Siti Aulia Rif'an, Mochammad riny Chandra, riny Ristika Handarini Rosnizar, Rosnizar Sari, Nisa Setianingsih, Dias Sofyan M. Saleh Sudarto Sudarto Sugeng Hari Wisudo Suhartono Suhartono SURI AMILIA, SURI Syakri, Sila Abdullah T.L Yusuf Tengku Putri Lindung Bulan, Tengku Putri Tengku Putri Lindung Bulan, Tengku Putri Lindung Tuty Laswardi Yusuf Vevi Sunarti W. Marlene Mesang-Nalley Waru Djuriatno Wilmientje Marlene Mesang Nalley Wilmientje Marlene Mesang-Nalley Winar, Sri Yulnawati - Yulnawati . YULNAWATI YULNAWATI