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VARIATION OF SEED PRODUCTION AND VIABILITY IN A FULL-SIB TRIAL OF Melaleuca cajuputi sub sp. cajuputi IN GUNUNGKIDUL YOGYAKARTA Baskorowati, Liliana; Susanto, Mudji; Prasetyono, Prasetyono; Kartikawati, Noor; Rimbawanto, Anto
Indonesian Journal of Forestry Research Vol 9, No 2 (2012): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.866 KB)

Abstract

Family variation of capsule production and the seed viability in the Paliyan full-sib trial of Melaleuca cajuputi subsp. cajuputi, at Gunungkidul, Yogyakarta, were observed. The full-sib trial was designed as Incomplete Block Design, consisting of 39 families; six individual as tree plot and replicated in eight blocks. Height and diameter at breast height were assessed to identify the correlation between capsule category and growth performance. The capsule production was assessed visually to one of the capsule categories: “0” for none; “1” for light; “2” for medium and “3” for heavy. Results showed that each family of M. cajuputi sub sp. cajuputi equally contributed  to the seed production.   Progeny analysis showed that the seed productions were not strongly under genetic control (h2i) = 0.12. This study also found positive correlation between the flowering strength (the flower production levels) and the number of capsule (R2=0.279). However, there were no significant differences between the flowering levels and the viability of M. cajuputi sub sp. cajuputi. Mean seed viability was 31%,  there were no significantly differences of capsules production between trees having high flowering intensity and low flowering intensity. Low seed viability was assumed due to the unsynchronicity of flowering, leading to the low levels of outcrossing rate. Therefore, selection of families with synchronicity of flowering was recommended to establish a seed orchard.
HUBUNGAN KEKERABATAN ANTAR POPULASI JATI (Tectona Grandis,Linn.F.) BERDASARKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) Widyatmoko, AYPBC; Rimbawanto, Anto; Chasani, Abdul Razaq
Jurnal Pemuliaan Tanaman Hutan Vol 7, No 3 (2013): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.822 KB) | DOI: 10.20886/jpth.2013.7.3.151-166

Abstract

Information on genetic relationship amongst teak (Tectona grandis Linn.f) populations in Indonesia is important, in order to maintain the high genetic diversity of the species and to ensure the origin of commercial planting materials. In this study, 94 loci from 25 RAPD primers were used for analyzing genetic diversity and genetic relationship among 30 populations of teak which collected from provenance trial in Bojonegoro and its populations in Sulawesi. Mean genetic diversity (h) of the 30 populations was 0.184, and mean genetic distance between populations (D) was 0.441. Based on cluster analysis, 30 populations of teak were divided into 3 groups. The first group consisted of Burma, the second group consisted of Jawa, India, Indochina and Thailand populations, and the third group consisted of all  populations in Sulawesi. High genetic distance between Jawa’s and Sulawesi’s populations can be used to  differentiate seed/seedling from both regions.
Improvement of Seed Orchard Management Based on Mating System of Cajuputi Trees Kartikawati, Noor Khomsah; Naiem, Mohammad; Hardiyanto, Eko Bhakti; Rimbawanto, Anto
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Breeding plan of cajuputi in Indonesia is aimed to increase plantation productivity of oil yield and 1.8 cineole content. Seed orchard of cajuputi at Paliyan, Gunungkidul, established using selected and genetically improved materials, has been producing seeds for operational plantation. This seed orchard would perform optimally if the mating systems of all individuals contribute to the inheritance of all genetic potential of the offsprings. Therefore, investigation of the mating systems of cajuputi was indispensible. The study has been carried out on 10 selected mother trees and the 24 offsprings of each mother trees using 8 microsatellite markers of nuclear DNA, namely Hin-2 (100-132 bp), Hin-4 (79-114 bp), Hin-5 (128-148 bp), Hin-7 (136-224 bp), Sal-1 (93-99 bp), Sal-3 (118-219 bp), Xho-1 (96-111 bp) and Xho-4 (150-216 bp), respectively. The result showed relatively high genetic variation of the offspring (HE=0.602, HO=0.594) originated from parent trees in the seed orchard. Parent trees tend to outcross(tm=0.951, ts=0.806), although seeds originated from biparental inbred (tm – ts = 0.145) and correlated paternity(rp=0.098) have also been observed. This genetically viable population could maintain its reproduction fi tness forshort term and adapt to the dynamic environmental changes for long term.Key words: mating system, cajuputi, seed orchard, microsatellite
Karakterisasi Morfologi dan verifikasi DNA Ganoderma philippii penyebab busuk akar Acacia mangium Puspitasari, Desy; Rimbawanto, Anto; Hidayati, Nur
Jurnal Pemuliaan Tanaman Hutan Vol 3, No 2 (2009): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1342.018 KB) | DOI: 10.20886/jpth.2009.3.2.83-94

Abstract

The incidence of rot disese has inflicted substantial damage to some plantation of Acacia mangium in Sumatera and Kalimantan. As soil-borne pathogen, Ganoderma is commonly found in the tropic and in Indonesia the disease also couse significacant losses to rubber and soil palm plantation. To help control the disease, appropriate identification of the fungi cousing the disease and other field characteritics are critical. Morphological characteistics applied in this study has proved to be effective to identify the pathogen. The disease is predominantly caused by G. philippii. The result of morphological identicication was verified with the DNA sequence of the same fungus and confirm the accuracy of the morphological characteristics. Other fungi that attack root of Acacia mangium are G. australe and Phellinus spp.
KERAGAMAN POPULASI Eusideroxylon zwageri KALIMANTAN TIMUR BERDASARKAN PENANDA RAPD Rimbawanto, Anto; Widyatmoko, AYPBC; Harklngto, Harklngto
Jurnal Penelitian Hutan Tanaman Vol 3, No 3 (2006): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1433.101 KB) | DOI: 10.20886/jpht.2006.3.3.201-208

Abstract

Eusideroxylon zwageri  atau ulin adalah kayu bemilai ekonomi tinggi dan telah mengalami eksploitasi yang intensif sehingga keberadaan tegakan ulin di hutan alam semakin langka. Penelitian ini bertujuan mempelajari keragaman genetik populasi ulin di Kalimantan Timur guna membantu program konservasi genetik, dengan menggunakan penanda RAPD. Sampel daun dikumpulkan dari 5 populasi dan dianalisa menggunakan 19 primer RAPD  yang menghasilkan 48 lokus polimorfik. Nilai keragaman  genetik rerata dalam populasi sebesar 0.3564 sedangkan keragaman antara populasi 0.0415. Analisis AMOVA menunjukkan  bahwa 96% dari keragaman genetik terdapat di dalam populasi, sedang sisanya ada di antara populasi. Analisis klaster menghasilkan dua kelompok populasi yaitu TN Kutai, Meratus, S.Wain dan Semboja, sedang populasi Lempake satu kelompok tersendiri.
VARIATION OF SEED PRODUCTION AND VIABILITY IN A FULL-SIB TRIAL OF Melaleuca cajuputi sub sp. cajuputi IN GUNUNGKIDUL YOGYAKARTA Baskorowati, Liliana; Susanto, Mudji; Prasetyono, Prasetyono; Kartikawati, Noor; Rimbawanto, Anto
Indonesian Journal of Forestry Research Vol 9, No 2 (2012): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.866 KB) | DOI: 10.20886/ijfr.2012.9.2.73-80

Abstract

Family variation of capsule production and the seed viability in the Paliyan full-sib trial of Melaleuca cajuputi subsp. cajuputi, at Gunungkidul, Yogyakarta, were observed. The full-sib trial was designed as Incomplete Block Design, consisting of 39 families; six individual as tree plot and replicated in eight blocks. Height and diameter at breast height were assessed to identify the correlation between capsule category and growth performance. The capsule production was assessed visually to one of the capsule categories: “0” for none; “1” for light; “2” for medium and “3” for heavy. Results showed that each family of M. cajuputi sub sp. cajuputi equally contributed  to the seed production.   Progeny analysis showed that the seed productions were not strongly under genetic control (h2i) = 0.12. This study also found positive correlation between the flowering strength (the flower production levels) and the number of capsule (R2=0.279). However, there were no significant differences between the flowering levels and the viability of M. cajuputi sub sp. cajuputi. Mean seed viability was 31%,  there were no significantly differences of capsules production between trees having high flowering intensity and low flowering intensity. Low seed viability was assumed due to the unsynchronicity of flowering, leading to the low levels of outcrossing rate. Therefore, selection of families with synchronicity of flowering was recommended to establish a seed orchard.
GENETIC DIVERSITY OF Dendrocalamus asper IN JAVA REVEALED BY RAPD MARKERS Rimbawanto, Anto
Indonesian Journal of Forestry Research Vol 3, No 1 (2006): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.837 KB) | DOI: 10.20886/ijfr.2006.3.1.67-74

Abstract

Dendracalamusasper is  one of  the most widely use and widely distributed bamboo  species in Indonesia. A genetic conservation plot has been established to maintain genetic diversity of the species. A total of  115 samples  were collected from 12 sites throughout Java. Genetic diversity was revealed using 31   RAPD  primers  producing  64 polymorphic  bands.  A total  of  81  different  multi-locus genotypes (genets) were identified between 115 samples. TI1e total number of multi-locus genotypes in all populations ranged from 3 to 9 (mean 6.5),  indicating that all populations consisted of multiple genotypes. Mean genetic diversity was 0.04, (ranging from the lowest 0.009 in Kuningan population to the highest 0.144  in Purwokerto population). The Simpsons diversity index indicated  that the most population composed of  unique genotype. Geographical differences did not correlate with genetic cluster.
Improvement of Seed Orchard Management Based on Mating System of Cajuputi Trees Kartikawati, Noor Khomsah; Naiem, Mohammad; Hardiyanto, Eko Bhakti; Rimbawanto, Anto
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.7865

Abstract

Breeding plan of cajuputi in Indonesia is aimed to increase plantation productivity of oil yield and 1.8 cineole content. Seed orchard of cajuputi at Paliyan, Gunungkidul, established using selected and genetically improved materials, has been producing seeds for operational plantation. This seed orchard would perform optimally if the mating systems of all individuals contribute to the inheritance of all genetic potential of the offsprings. Therefore, investigation of the mating systems of cajuputi was indispensible. The study has been carried out on 10 selected mother trees and the 24 offsprings of each mother trees using 8 microsatellite markers of nuclear DNA, namely Hin-2 (100-132 bp), Hin-4 (79-114 bp), Hin-5 (128-148 bp), Hin-7 (136-224 bp), Sal-1 (93-99 bp), Sal-3 (118-219 bp), Xho-1 (96-111 bp) and Xho-4 (150-216 bp), respectively. The result showed relatively high genetic variation of the offspring (HE=0.602, HO=0.594) originated from parent trees in the seed orchard. Parent trees tend to outcross(tm=0.951, ts=0.806), although seeds originated from biparental inbred (tm – ts = 0.145) and correlated paternity(rp=0.098) have also been observed. This genetically viable population could maintain its reproduction fi tness forshort term and adapt to the dynamic environmental changes for long term. Key words: mating system, cajuputi, seed orchard, microsatellite
GENETIC DIVERSITY OF Dendrocalamus asper IN JAVA REVEALED BY RAPD MARKERS Rimbawanto, Anto
Indonesian Journal of Forestry Research Vol 3, No 1 (2006): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.837 KB) | DOI: 10.20886/ijfr.2006.3.1.67-74

Abstract

Dendracalamusasper is  one of  the most widely use and widely distributed bamboo  species in Indonesia. A genetic conservation plot has been established to maintain genetic diversity of the species. A total of  115 samples  were collected from 12 sites throughout Java. Genetic diversity was revealed using 31   RAPD  primers  producing  64 polymorphic  bands.  A total  of  81  different  multi-locus genotypes (genets) were identified between 115 samples. TI1e total number of multi-locus genotypes in all populations ranged from 3 to 9 (mean 6.5),  indicating that all populations consisted of multiple genotypes. Mean genetic diversity was 0.04, (ranging from the lowest 0.009 in Kuningan population to the highest 0.144  in Purwokerto population). The Simpsons diversity index indicated  that the most population composed of  unique genotype. Geographical differences did not correlate with genetic cluster.
KERAGAMAN GENETIK EMPAT POPULASI lntsia bijuga BERDASARKAN PENANDA RAPD DAN IMPLIKASINYA BAGI PROGRAM KONSERVASI GENETIK Rimbawanto, Anto; Widyatmoko, AYPBC
Jurnal Penelitian Hutan Tanaman Vol 3, No 3 (2006): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2182.779 KB) | DOI: 10.20886/jpht.2006.3.3.149-154

Abstract

Intsia bijuga atau merbau merupakan jenis kayu bemilai ekonomi tinggi dan telah mengalami eksploitasi  yang intensif. Penelitian ini bertujuan mempelajari keragaman genetik populasi merbau guna membantu penyusunan strategi konservasi genetik, dengan menggunakan penanda RAPD. Sampel daun dikumpulkan dari 4 populasi dan dianalisa menggunakan 15 primer RAPD yang menghasilkan 77 lokus polimorfik. Rata-rata lokus polimorfik per primer adalah 5.1. Nilai keragaman genetik rerata dalam populasi sebesar 0,296 sedangkan keragaman antara populasi 0.141. Analisis klaster membagi keempat populasi menjadi dua kelompok populasi yaitu Carita dan Manokwari pada kelompok pertama, sedangkan kelompok kedua terdiri dari populasi Temate dan Nabire. Pembagian kelompok antara Manokwari  dan Nabire lebih memperjelas pembagian Papua menjadi 6 wilayah geogenetik.