Articles

Extraction and analysis of Phytosterol from wheat germ (Triticum sp.) Marliyati, Sri Anna; Syarief, Hidayat; Muchtadi, Deddy; Darusman, Latifah K; Rimbawan, Rimbawan
Jurnal Teknologi Dan Industri Pangan Vol 16, No 1 (2005): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.496 KB) | DOI: 10.6066/458

Abstract

Phytosterol may reduce the absorption of cholesterol, and used for preventing atherosclerosis. It is limited in soybean, but potentially abundant in wheat germ. Research on the utilization of wheat germ sterol had not been reported so far. Many aspects of germ sterol extraction from wheat germ and its characteristics were still unknown. In this research, the best extraction method, kinds and content of phytosterol from wheat germ were investigated. This research consisted of two steps: (1) extraction of phytosterol directly form whole germ and ground germ using hexane, and indirect extraction through germ oil using hexane and mixed solvent of hexane and ethanol, and direct extraction from ground germ using ethanol; (2) analysis of the type and content of phytosterol in the crude extract through the following steps: preparation of crude extract, fractionation, and analysis. Results showed that indirect extraction through germ oil was considered as the best method which yielded 1.37% of phytosterol. The highest yield was obtained when extracted using a mixed solvent of hexane – ethanol 82:18. However, the odor of ethanol and hexane (gasoline like odor) was still detected. The solvent’s ratio of hexane to ethanol at 1:2 resulted better odor of the extract. Extraction of sterol using ethanol yielded 18.39% of sterol when the ratio of germ to ethanol at 1:10 (w/v) was applied. Results of quantitative analysis on the main component of crude extract of wheat germ sterol showed that the total content of sterol extracted with mixed solvent was higher than those extracted with ethanol. The ratio of hexane to ethanol at 1:1 (v/v) gave higher content of total sterol, stigmasterol and campesterol, whereas higher content of -sitosterol was produced at the solvent’s ratio of hexane to ethanol at 1:2 (v/v). Keywords: Wheat germ sterol, extraction, β-sitosterol, stigmasterol, campesterol
ABSORPSI β-KAROTEN SERBUK DAUN SINGKONG (Manihot Utilissima) KERING PADA ANAK PRASEKOLAH Almasyhuri, Almasyhuri; Khumaidi, M.; Muhilal, Muhilal; Rimbawan, Rimbawan
Jurnal Penelitian Gizi dan Makanan JILID 20 (1997)
Publisher : Jurnal Penelitian Gizi dan Makanan

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Abstract

Indonesia telah bebas dari masalah kurang vitamin A (KVA), tetapi jumlah anak balita dengan vitamin A serum marjinal (<20 ug/dl) masih tinggi. Salah satu program penanggulangan KVA di Indonesia adalah dengan peningkatan konsumsi sayuran hijau pada anak balita. Menurut de Pee, karoten sayuran hijau kurang dapat diabsorpsi karena berupa ikatan komplek yang kuat yang berada dalam kloro plas. Penelitian ini mempelajari pengaruh pembuatan serbuk kering daun singkong muda dan tua terhadap absorpsi β-karoten pada anak prasekolah. Penelitian dilakukan di Kotamadya Bogor, Jawa Barat. Daun singkong diberikan dalam bentuk masakan gulai, yang diberikan dalam diit makanan pagi, siang dan sore selama tiga hari. Kadar β-karoten dalam duplikat makanan yang dikonsumsi dan dalam tinja selama tiga hari dianalisis dengan HPLC. β-karoten yang diabsorpsi merupakan selisih β-karoten dalam duplikat makanan yang dikonsumsi dan β-karoten dalam tinja selama tiga hari. Absorpsi β-karoten serbuk daun singkong muda kering (DSMK) paling tinggi (37.9±5.2%) dibanding β-karoten serbuk daun singkong tua kering (DSTK) maupun daun singkong muda segar (DSMB) yang besarnya masing-masing adalah 36.8±9.2% dan 35.4±5.8%. β-karoten daun singkong muda segar (DSMB) paling kecil absorpsinya. Tetapi hasil uji anova β-karoten dari ketiga jenis daun singkong tersebut tidak ada perbedaan nyata (p>0.05). Kesimpulan dari penelitian ini ternyata pengeringan dan penghancuran menjadi serbuk belum dapat memperbaiki absorpsi β-karoten yang dikandung dalam daun singkong. 
PENGARUH PENGOLAHAN KEDELAI MENJADI TEMPE DAN PEMASAKAN TEMPE TERHADAP KADAR ISOFLAVON (EFFECTS OF SOYBEAN PROCESSING BECOMING TEMPEH AND THE COOKING OF TEMPEH ON ISOFLAVONES LEVEL) Utari, Diah M; Rimbawan, Rimbawan; Riyadi, Hadi; Muhilal, Muhilal; Purwantyastuti, Purwantyastuti
Jurnal Penelitian Gizi dan Makanan Vol 33, No 2 (2010)
Publisher : Jurnal Penelitian Gizi dan Makanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | http://ejournal.litbang.depkes.go.id/index.php/pgm/article/view/3119

Abstract

ABSTRACT Background: Research over the past two decades has provided significant clinical trial and epidemiological evidence for health benefits of the consumption of soybean-based foods. A health claim indicating that high soybean consumption is associated with a lower risk of coronary heart disease (CHD). Compositions of nutrient and non-nutrient in soybean have been examination and give the contribution on lower risk of CHD especially on improve of lipid profile. Isoflavones is a non-nutrient that abundant in soybean. Tempeh is fermented soybean that popular as Indonesian traditional food and content of isoflavones is greater than soybean. Objective: to study effect of soybean processing becoming tempeh and the cooking of tempeh on isoflavones level. Methods: The samples of this study is raw tempeh and steamed tempeh. The analysis of isoflavones has used high performance liquid chromatography (HPLC). Results: During soybean processing becoming tempeh, twice boiling of soybean produce result isoflavones 47.4 percent greater than once boiling. Steaming tempeh result minimized isoflavones reduction (13.3%). Although there is no dietary recommendation for individual isoflavones, may be great benefit in increased consumption of tempeh. [Penel Gizi Makan 2010, 33(2): 148-153]   Keywords: tempeh, processing, isoflavones
KANDUNGAN IODIUM PADA BEBERAPA BAHAN MAKANAN DI DAERAH PANTAI ENDEMIK DAN NON-ENDEMIK Gunanti, Inong Retno; Suhardjo, Suhardjo; Kusharto, Clara M.; Rimbawan, Rimbawan; Wirjatmadi, Bambang
Buletin Penelitian Sistem Kesehatan Vol 3, No 1 Jun (1999)
Publisher : Buletin Penelitian Sistem Kesehatan

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Abstract

Generally, Iodine Deficiency Discorders (IDD) is prevalent among people who live in mountain areas with low iodine content in the soil, drinkin-water and the foods-stuff. Recently, based on several studies, it is reported that the IDD cases are also found among people who live along coastal areas. No one report has explained the possible cause explicitly. The objective of this study is to analyze the iodine content of foods-stuff consumed in endemic and nonendemic coastal areas to explore the possible factors influencing IDD in villages along coastal areas. The study was conducted from April to August 1998 in the coastal area of Belimbing Village, Paciran sub district (as endemic coastal area) and Labuhan Village, Brondong sub district (as nonendemic coastal area), Lamongan Regency, East Java Province. The two villages are located along Java Sea. For this purpose 30 school children were selected as samples from each village ("Sekolah Dasar Belimbing I"    and "Sekolah Dasar Labuhan 117". The food consumption data were collected by using food frequency questionnaire and the iodine content in foods-stuffs were measured quantitatively in laboratory. The result of this study shows that the iodine content in most of the foods-stuff in Belimbing Village is lower than Labuhan Village. The difference is around 2.5- 4.5 microgram/100g for sea production, and around 0.025-0.59 microgram/100g for vegetables and fruits.
KETERBATASAN RESPON HEMOGLOBIN DAN SENG SERUM TERHADAP SUPLEMENTASI SENG (IN) DAN BESI (FE) PADA ANAK USIA 6-24 BULAN Riyadi, Hadi; Sibarani, Sudjana; Rimbawan, Rimbawan; Muhilal, Muhilal
Media Gizi dan Keluarga Vol 28, No 1 (2004): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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Abstract

ABSTRACT. The objective of  this study  was to investigate the efficacy of  iron and zinc supplementation on anemic status and zinc status of children aged 6-24 months. The study was a double-blind, randomized, placebo-controlled trial in which 173 children aged 6-24 months were randomly assigned to receive 15 mg Fe+ 15 mg Zn daily 30 mg Fe+30 mg Zn weekly or placebo for 5 months. As expected, no significant difference in the baseline hemoglobin and serum zinc concentration and nutrient intake between the three supplementation groups. In the children aged 6-24 months, micronutrient deficiencies were prevalent. Almost 50% of the children were anemic, and 20% of them were zinc deficient. The anemic children had a 2.5-fold greater risk of  zinc deficient than did the non-anemic children. This could indicate the existence of concurrent nutritional deficiencies. At the end of the 5mo supplementation period, the daily and weekly Fe+Zn supplementation tend to increased hemoglobin concentrations, but there were no significant difference compared with the placebo. The daily and weekly Fe+Zn supplementation did not significantly affect serum zinc concentration compared with the placebo.   Keyword: suplementasi, anemia, besi dan seng.
PENGARUH STEROL LEMBAGA GANDUM (Triticum sp.) TERHADAP PROFIL LIPIDA DARAH TIKUS Marliyati, Sri Anna; Syarief, Hidayat; Muchtadi, Deddy; Darusman, Latifah; Rimbawan, Rimbawan; Priosoerjanto, Bambang Pontjo
Media Gizi dan Keluarga Vol 28, No 2 (2004): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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ABSTRACT. The role of fitosterol in preventing cholesterol absorption and reducing atherosclerosis risk has been reported. This research was conducted to evaluate the effect of wheat germ sterol on the concentration of total cholesterol, LDL, HDL, triglycerides, and lesion formation in mice. In this research work. Sprague Dawley mice at the age of 2 months  (productive age) were used A random block design was employed with the following treatment on each experimental unit: Po (basal diet, negative contra/), PI (basal diet with cholesterol added, positive control), P2 (basal diet with cholesterol and ground wheat germ added), P3 (basal diet with cholesterol and wheat germ oil added). P4 (basal diet with cholesterol and wheat germ sterol added). and P5 (basal diet with cholesterol and wheat germ sterol supplemented margarine). Each mouse was d.esigned to intake 0.0343 g ofsterol daily, except those of negative control (Po) and positive control (PI). In order to increase cholesterol concentration in blood serum ofmice, 20 mglkg-body weight per day ofpropyltiourasil (PTU) was added into each diet. A set oftests was carried out to observe the concentrations of total cholesterol. LDL, HDL, and tryglycerides in blood serum of test animals, and mouse aorta micrographs. Results showed that wheat germ sterol could prevent the increase ofcholesterol in the blood ofmice. Feeding with sterol (P4) or sterol-supplemented margarine (P5) prevented the increase of total cholesterol after I month of experimentation, however, only P4 sustained the same condition after 2 months ofexperimentation. Feeding with wheat germ sterol (P4) was also the only treatment that prevented the increase of LDL cholesterol after 2 month of experimentation, and it was not different significantly with negative control (Po). Results of experimentation also showed that the concentration of HDL in serum blood of mice was not affected by wheat germ sterol. Unsurprisingly, there was no lesion ofatherosclerosis observed in all treatments used in this research. This result confirmed that mouse is resistant to atherosclerosis. Keywords: wheat germ sterol, mouse, total cholesterol, LDL, HDL, triglycerides. atherosclerotic lesions
PENGARUH INTERVENSI MAKANAN KUDAPAN TERHADAP PENINGKATAN KADAR GLUKOSA DARAH DAN DAYA INGAT ANAK SEKOLAH DASAR Kustiyah, Lilik; Syarief, Hidayat; Hardinsyah, Hardinsyah; Rimbawan, Rimbawan; Suradijono, Sri Hartati
Media Gizi dan Keluarga Vol 30, No 1 (2006): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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ABSTRACT. The objective of this study was to analyze the effect of snack intervention on blood glucose level and memory improvement ofelementary schools students, Subjects of this study were 184 students offour (4) elementary schools (grade 6, 5 and 4) at Bogor District, West Java. The study employed a quasi-experimental design and followed experimental procedures to control the subjects food intake and motoric activity during the study period At the day of intervention, both control and interventions subjects were ordered not to have breakfast at home. Interventions subjects were provided with snack (buras, at 10.00 AM) which contained 381.7 kcal energy and 5 g protein, but controls subjects were not. Two types ofpsychological test (word andfigure) were applied twice (at 09.00 and 11.00 AM). Then, at the same time, subjects blood was taken to determine blood glucose, haemoglobin, and hematocrite levels. Interviews with subjects and their mothers were carried out to collect socioeconomic data and dietary intake. Result of the study indicated that snack intervention increased significantly (p<O.OI) blood glucose level (20.8 mgld/) approximately 1 hour after snack given. Blood glucose level significantly (p<O.OI) affected the words and figures memory performance. The higher the blood glucose level the better the memory performance. Keywords: Snack intervention, blood glucose level, memoryperformance, Elementary School Students
PENGARUH SUPLEMEN IODIUM DAN BETA KAROTEN TERHADAP STATUS IODIUM DAN STATUS GIZI IBU HAMIL DI DAERAH ENDEMIK GAKI Lamid, Astuti; Rimbawan, Rimbawan; Khomsan, Ali; Kusharto, Clara Meliyanti; Muhilal, Muhilal
Media Gizi dan Keluarga Vol 31, No 2 (2007): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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Abstract

ABSTRACT. Iodine Deficiency Disorders (IDD) prevalence ofpregnant women is 16% and this would have consequence of risk during delivery and  nutritional status of the children. The aims ofthis study was to measure the effect ofiodized oil and beta carotene on the nutritional, iodine and thyroid stimulating hormone (TSH) status of pregnant women and to determine nutrition, iodine and cyanide intakes from food consumed by pregnant women. Research design ofthe study was quasi experiment. Pregnant women on the first trimester were chosen and divided into three groups. The first group was given iodized oil at 200mg iodine, the second group was treated with iodized oil at 30mg iodine every month for 6 months, and the third group was given as group 2 plus beta-carotene at 30mg each month for six months. Data collected were nutrients, iodine, and cyanide intake from food consumed and the parameter measured were serum TSH, urine iodine excretion (UIE), body weight and upper arm circumference. Chi Square, ANOVA and Kruskall Wallis were applied for statistical analysis. Iodine consumed by pregnant women was closed to the recommended daily allowance (RDA). iodized oil supplement could increase the UIE for 2 times at trimester 3 ofthe pregnancy. Furthermore, the treatment could redure the TSH value during pregnancy. The supplement could supply iodine during pregnancy. Keywords: iodine, hipothyroid, beta caroten, nutritional status
KONSUMSI PANGAN DAN SENG, SERTA DETERMINAN STATUS SENG IBU HAMIL DI KECAMATAN LEUWILIANG DAN CIBUNGBULANG, KABUPATEN BOGOR Hayati, Aslis Wirda; Hardinsyah, Hardinsyah; Rimbawan, Rimbawan
Media Gizi dan Keluarga Vol 31, No 2 (2007): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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ABSTRACT. The main objectives of this study were to analyze zinc status, nutrient dietary, quality zinc content, zinc intake, and determinant factor of zinc status. The research was based on previous study on "Effect of Multi-nutrients Fortified Food Supplementation for Pregnant Women and Child Growth and Development" and  some additional data of food  zinc content. Logistic regression was applied for determinants analysis. The results show that zinc serum level was 0.9±04mgll. The prevalence of zinc deficiency was 21.8%. Food intake ofpregnant women was very low (30.8% of suggested serving). Zinc content offood ranged from O.2mg (fried tempeh) to 22.9mg (local snail) per 100g oft he dried foods. Zinc intake was 6.3mglday, similar to 32.8% recommended dietary allowance (RDA). The subjects who have zinc intake lower than its RDA (l9.3mglday) were 99.2%. The determinant ofzinc status was household income . Keywords: food consumption, zinc intake, zinc status, pregnant women,
PEMBERIAN PANGAN YANG DIFORTIFIKASI ZAT MULTI GIZI MIKRO DAN PENGARUHNYA TERHADAP STATUS ANEMIA DAN PERTAMBAHAN BERAT BADAN IBU HAMIL Prihananto, V; Rimbawan, Rimbawan; Astawan, Made; Sulaeman, Ahmad
Media Gizi dan Keluarga Vol 30, No 2 (2006): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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ABSTRACT. The objetive of this study was to evaluate the effect offeeding with multimicronutrients fortified foods on anemia status and weight gain ofpregnan womrql. This study was carried out in three sub-districts in Bogor, i. e.: Leuwiliang, Leuwisadeng and Ciampea. A double blind randomized control trial was assigned on this study. About 210 pregnant women from these sub-districts were selected for this study and devided into 2groups, (1) 70 pregnant women received fortified foods with multi-micronutrients i.e. iron, iodine, zinc, folic acid, vitamin C, and vitamin A, and (2) 70 pregnant mothers received nonfortifiedfoods, each for 6 mounths. Product chosen as intervention carrier consisted of vermicelli, milk, and biscuit. Distribution ofproducts were carried out every week to thetarget pregnant women. The results of the study showed: (a) intervention could reduce prevalence ofanemia as much as 28% compared with placebo (b) Intervention affected the micronutrients consumption andfulfilled: iron 55,5% RDA, vitamin A 65% RDA, vitamin C 126% RDA, folat 15,3% RDA, zinc 26,7% RDA, iodium 26,3%, (c) intervention didntinfluence weight gain during pregnancy.
Co-Authors Adi Teruna Effendi Adi Winarto Agustino Agustino, Agustino Ahmad Sulaeman Ainia Herminiati, Ainia Ali Khomsan Almasyhuri Almasyhuri Angga Hardiansyah Ascobat, Purwantyastuti Aslis Wirda Hayati Astuti Lamid Bambang Pontjo Priosoerjanto Bambang Wirjatmadi Bibi Ahmad Chahyanto Budi Setiawan Cesilia Meti Dwiriani Clara M. Kusharto Clara Meliyanti Kusharto Dadan Rohdiana Dadang Sukandar Damayanthi, Evi Damayanti, Rinda DEDDY MUCHTADI Deni Elnovriza DEWI APRI ASTUTI Dewi Kusumawati, Dewi Diah M Utari Diah M. Utari Diffah Hanim Dodik Briawan Drajat Martianto Efendi, Adi Teruna Ekayanti, Ikue Ekowati Handharyani Evy Damayanthi Faisal Anwar Firdaus Firdaus Hadi Riyadi Hardinsyah Hardinsyah Harianti, Rini Hidayat Syarief Ikeu Ekayanti Ikeu Tanziha Inong Retno Gunanti ISWAHYUDI ISWAHYUDI Katrin Roosita Kharisma Tamimi Latifah Darusman latifah K Darusman Leily Amalia Lilik Kustiyah Linar Zalinar Udin, Linar Zalinar M. Khumaidi MADE ASTAWAN MARIA BINTANG Mira Dewi Muhilal Muhilal Mury Kuswari Naufal Muharam Nurdin Ni Ketut Sutiari Nuri Andarwulan Purwantyastuti Purwantyastuti Puspo Edi Giriwono Putri Puncak Anjani Rahman, Dewi Rahmayani Rakhmawati FKR Ratu Diah Koerniawati Resita Nurbayani Restu Pertiwi Rizal M Damanik, Rizal M Rosyanne Kushargina Siti Madanijah Sri Anna Marliyati Sri Hartati Suradijono Sri Nur Amalia Sri Purwaningsih Subangkit, Mawar Sudjana Sibarani Suhardjo Suhardjo Susi Nurohmi Syartiwidya, Syartiwidya Trini Suryowati, Trini Tunggul Waloya V Prihananto Wiwin Winarsih