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Journal : BERITA BIOLOGI

IDENTIFIKASI VIRUS PENYAKIT JEMBRANA PADA SAPI BALI MENGGUNAKAN PENANDA MOLEKULER GEN env SU [Identification of Jembrana Disease Virus by Using a Molecular Marker of env SU Gene in Bali Cattle] Indriawati, Indriawati; Margawati, Endang Tri; Ridwan, Muhammad
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.055 KB) | DOI: 10.14203/beritabiologi.v12i2.534

Abstract

Up to present, detection of Jembrana disease virus has been identified through serological test. Advances in molecular biology has enabled to detect Jembrana disease virus earlier, quicker and more accurate by application of molecular markers.The aim of this study was to identify Jembrana disease by using molecular marker of env SU gene in Bali cattle.Total RNA of Jembrana disease virus (7732bp) was collected from spleen of Bali cattle suspected Jembrana disease by using RNEasy Protect Mini Kit (QIAGEN). A pair of specific primers was designed from Jembrana viral genome (env SU) that accessed through a GenBank with Accession Number of U21603.A kit of Access Quick RT-PCR System (PROMEGA) was used for Reverse-Transcriptase-PCR (RT-PCR). The RT-PCR products were visualized on 2% agarose gel.The result showed a single band with the size of ± 900bp in all samples. This size indicated that env SU gene was existed in the examined spleen samples. This finding suggests that a molecular marker could be used accurately to identify the env SU gene in JDV of Bali cattle.
Co-Authors - Sulaiman ., Mardawati Abdul Munir Achmad Fudholi Achmar Mallawa Ade Aditia Armi Adi Syahfutra Adnan Sauddin, Adnan Adrian Novigasa, Adrian Agung Mahar Rani Aidil Darma Setiadi, Aidil Darma AL IKHLAS " Alfikar " Amanah, Ulfah Nurul Amila Gadri Arlina Prima Putri, Arlina Prima Armayani, Hanifa Ayu Auradian Marta Bambang Pramono Baskoro, Galih Wahyu Darmawati Darwis Denny Sagita Rusdianto Diana Ningrum, Diana Edi Purnomo Ediyono, Suryo Elviza " ENDANG TRI MARGAWATI Fadil Oenzil Fadil, Jazuli Fadil, Jazuli Faisal . Farid Lubis, Mahbud Fathoni, Rizal Fatihah, Ishma Febry, Muhamad Fernandes Pakpahan, Fernandes Galih Ujiantoro, Galih Hamelinda, Fitri Haq, Ramiz Ansharil Harahap, Isnaini Harahap, Pangeran Hardianti, Ratih Hayyu Dwi Putri Helda Yenni, Helda Henki Bayu Seta, Henki Bayu Ichwann Hastona, Ichwann Ilham Wahyudi Indradjaja, Indradjaja Indriawati, Indriawati Iqbal Maulana, Iqbal Iqbal Wahid, Iqbal Irfan Taufik, Irfan Irmayanti Irmayanti, Irmayanti Irwan, Muh. Isril " Junizan " Kamal " Kasman Kasman Kaswari . Khairiah, Hanifa Khairul Anwar Laode Muh. Golok Jaya, Laode Muh. Golok Lokaria, Aldian Mulyanto Lubis, Kamaluddin M. Banda Selamat, M. Banda Mahardeka Tri Ananta, Mahardeka Tri Mahmudah, Qonitatul Mandala, Eka Praja Wiyata Marya Ulfa Mauli Kasmi, Mauli Miftah Ardhillah Fajri, Miftah Ardhillah Mitriani, Shelmia Muhammad Firdaus Muhammad Muhammad Muhammad Rizki Muhammad Walihuddin Mukhtar Mukhtar mukti Zainuddin Munawir, Ahmad Mursalin, Muhammad Mursalin, Muhammad Musbir Musbir Musta'in, Musta'in Nadya Vinandari Nawir Rasidi Nida’uljanah, Hanifah Novi Triayanti Noviarni ., Noviarni Noviarni, . nugraha, noviyanti Nugroho, Edy Prasetyo Nur Rahmah Nurhuda, Yeni Agus Nurul Muslihat, Nurul Oktari, Ahmad Rezha Pangestu, Danang Aji Pramuda N.S, Muhammad Prita D. S, Septarina pujowati, penny Puspa Arum, Enggar Diah Putri, Andi Rani Sahni Putri, Andi Rani Sahni Putri, Dewi Eka Rachmat Hidayat Rahayu Rahayu Rahmat Hidayat Ramadhani, Siti Reno Efaldi Reristiani Jantia Reski Lestari Richo Rahgia Syahputra Riki Syaputra, Riki Risnawati Ibnas, Risnawati Rizki Ananda Rizky, Said Muhammad Rizona, Firnaliza Robby Setiawan Robiatun Adawiyah, Robiatun Roesyandi Nan Arief, Roesyandi Nan Rosdianwinata, Eka Rudi Febrianda, Rudi Rusdiansyah Rusdiansyah S. Sudarno, S. Safruddin, . Sahat Siagian Samsul Bahri Sandi, Hengki Erik Santoso, Kukuh Aris Saputra, Dede Sarip Hidayat, Sarip Sofian, Sofian Sri Rahayu Sugianto Sugianto Supra Yogi, Supra Surya Fernando, Surya Suryono, Ryan Randy Sutardi Sutardi Syamsuri Syamsuri Telanai, Yudi Theresia Wati, Theresia Tjokorda Gde Dharmayuda Tohari, M Imam Tria Febrina Triyanti Nurul Hidayati Try Azisah Nurman vito siregar, Akbar ihza Wira Gotera Yandri Pratama Yulianto Yulianto Zainal Arifin Zamira Ulfa ZURAIDAH ZURAIDAH