Wibi Riawan
Departemen Biokimia dan Biomolekuler, Fakultas Kedokteran, Universitas Brawijaya

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ANALISIS LOSS OF HETEROZYGOSITYGEN RAR β2 PADA PATOGENESIS KANKER PAYUDARA Muliartha, I Ketut Gede; Riawan, Wibi; Hidayat, Asnah; Armania, Fitri
Jurnal Kedokteran Brawijaya Vol 24, No 2 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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The aim of this research was to investigate the event of loss of heterozygosity of retinoid acid receptor (RAR β2) gene on the chromosom 3p24 in the pathogenesis ofbreast cancer. The breast cancer is the most malignant tumor that leads the second cause of death among the female. The recent concept of the theory of pathogenesis breast cancer is molecular genetic approaches such as apoptosis gene, oncogen, suppressorgene, and growth promoting factor gene. However, the diagnostic and therapy of breast cancer are still not satisfied. The lost or decreased in the expressionof RAR  β2gene on 3p24 chromosome via loss of heterozygosity (LOH) mechanism are the role of pathogenesis of breast cancer. Design of the research is by experimental and observational. Human female breastcancer and blood samples were gained from public hospital RSUD dr. Saiful Anwar, Malang. DNA was extracted from lymphocyte and tissue using Macherey-Nagel system kit. Loss of heterozygosity (LOH) incident in 3p24 chromosome was detected by PCR and Chromogenic in situ Hybridization (CISH) method.
HUBUNGAN ANTARA DERAJAT OBSTRUKSI SERANGAN AKUT ASMA DENGAN KADAR IgE, IFN- DAN IL-4 TOTAL SERUM Kusuma, HMS. Chandra; Kalim, Kusworini Handono; Riawan, Wibi; Muid, Masdar
Jurnal Kedokteran Brawijaya Vol 20, No 2 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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ABSTRACT Acute bronchial asthma is a common medical emergency the world over. There now exists compelling evidence of a role for Th2-Th1 paradigm in the pathogenesis of asthma in children. We hypothesized that childhood asthma is associated with the activation of  Th2-Th1 lymphocytes whose products regulate at least in part, the expression of IgE, IFN-γ and IL-4 and thereby disease severity. Our aims, therefore were to compare the level of total IgE, total IL-4 and total IFN-γ in serum from asthmatic and non asthmatic control children matched for age and sex, and to attempt to correlate the IgE, IL-4, and IFN-γ total level of serum in the asthmatics children with disease severity. Fifty one children with acute asthma exacerbation were compare with thirty one no asthmatics normal children matched for age and sex. The level of IgE, IL-4, and IFN-γ were measured using Elisa. Asthma severity was assessed by a symptom score and spirometri. The level of IgE and Il-4 in the serum of the asthma were elevated as compared to the non asthmatic normal controls, whereas the IFN-γ was decreased. There was a significant correlation between elevated levels of IgE and IL-4 with the acute asthma exacerbation severity, whereas the decreased level of IFN-γ was not. Conclusions. The increased levels of IgE , IL-4 and the ratio of  IL-4/IFN-γ play a crucial role in the acute asthma exacerbation severity.
DISTRIBUSI MITOKONDRIA DAN EKSPRESI BAX PADA HUVEC’s YANG MENGALAMI APOPTOSIS AKIBAT INDUKSI OX-LDL Riawan, Wibi; Wibowati, Samodrijanti
Jurnal Kedokteran Brawijaya Vol 22, No 2 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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ABSTRACT It had been revealed that endothelial dysfunction which is caused by oxydized LDL is related to TNF Receptor Apoptosis Including Ligand (TRAIL) mechanism. However, there is study to see the involvement of mitochondrial mecanism in Ox-LDL endothelial cells dysfunction. This study was done to see the effect of Ox-LDL treatment on apoptosis of HUVEC’s culture  whether  associated  with  the  distribution  of  mitochondria  or  BAX  expression,  the  main  protein  mitochondria apoptosis mechanism. HUVEC’s culture was divided into two groups: control group and treatment group ( 50 mg/ml Ox-LDL for 24 hours). Doublestaining for DNA fragmented (TUNEL) and BAX expression were done respectively, as well as immunochemistry  using  monoclonal  Ab  of  mitochondria.  In  the  control  group  mitochondria  was  distributed  firmly  on cytoplasma of endothelial cells. In contrast, mitochondria were only found near nuclear membrane cells on treatment group  (this  future  usually  found  on  divided  cells) there  was  also  an  increasing  of  BAX  expression  and  a  mount  of apoptotic cells on treatment group compared with control group. It can be concluded that mitochondria is involved in apoptosis of Ox-LDL endothelial cells in order to serve the abundant energy. Keywords: Mitochondria distribution, Bax expresson, Apoptotic, Ox-LDL
PENGARUH PEMBERIAN OX-LDL TERHADAP AKTIVASI NF-кB DAN PPARγ γγ γSERTA APOPTOSIS PADA KULTUR HUVEC’s Riawan, Wibi; Wibowati, Samodijanti
Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Oxidized LDL (ox-LDL) causes activation and dysfunction of endothelial cells through NFκB activation after binding process of ox-LDL to LOX-1. One of ox-LDL components had been known as the activator of PPARγ. This study was done to see the effect of ox-LDL on NFκB and PPARγactivation and apoptosis of HUVEC’s culture. HUVEC’s culture was divided into 3 groups : control (without any treatment) and 2 groups which were treated with ox-LDL 25ug/mL and 50ug/mL. Activation of NFκB and PPARγwere determined by immunodoublestaining using mouse monoclonal anti NFκB and mouse monoclonal anti PPARγafter 30 minutes exposure of ox-LDL. The expression of TNFαand apoptotic cell were determined by immunodoublestaining using  TUNEL fragmented DNA labeling and goat polyclonal anti human TNFαafter 24 hours exposure of ox-LDL. HUVECs culture that had been treated with 25  µg/ml and 50µg/ml of ox-LDL, showing activation of NFκB but not PPARγ. This treated endotel showed apoptotic characteristic which had conformed with DNA fragmented. These cells also showed the increase of TNFαexpression on the cytoplasm. Ox-LDL could increase translocation of NFκB gen transcription to nucleus followed by the increase of TNFαthat can cause apoptotic of the cells. Key words: ox - LDL, Endothel, NF-κB, PPARγ, Apoptosis
CYCLOOXYGENASE-2 (COX-2) EXPRESSION ON TESTIS CONNECTIVE TISSUE OF Rattus norvegicusAFTER TREATMENT WITH BETEL NUT EXTRACT (Areca catechu) Akmal, Muslim; Riawan, Wibi
Jurnal Kedokteran Brawijaya Vol 23, No 3 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Betel nut contains alcaloids such as arecoline, arecaine, arecaidine, arecolidine, guvacine, guvacoline and isoguvasine. Arecoline has ability to change gonad morph-function, including shape abnormality of sperm. This research was conducted to prove the ability of betel nut extract (Areca catechu) in causing apoptosis on testis connective tissue of Rattus novergicus. This research used male; 2-3 months age, 150-200 grams body weight of white rats Rattus norvegicus strain Wistar. The rats were divided into 5 groups in equalnumber, 3 rats respectively. They were a control group without treatment and 4 groups as treatment groups which were given doses of betel nut extract, i,e., 1, 2, 3  and 4 gram during seven days. The result showed that dose variation ofbetel nut extract could induction of COX-2 expression on rats (Rattus norvegicus) strain Wistar testicular seminiferous tubule. Keywords: Betel nut extract, Rattus norvegicus, testis, cyclooxygenase-2
The effect of four weeks restricted diet on serum soluble leptin receptor levels and adipocyte leptin receptor density in normoweight rattus norvegicus strain Wistar Indra, M. R.; Riawan, Wibi
Medical Journal of Indonesia Vol 15, No 3 (2006): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.784 KB) | DOI: 10.13181/mji.v15i3.229

Abstract

One of the five possible mechanisms of leptin resistance in human obesity is the defect in the leptin receptor (Ob-R). Evidence has accumulated that leptin-binding activity in human serum is related to a soluble form of the leptin receptor, and restriction of energy intake resulted a decrease in circulating leptin levels. Aim of this study is to examine the difference of serum soluble leptin receptor level and leptin receptor density in rat adipose tissue of adventitial aorta after four weeks treated with different restricted diets. Soluble leptin receptor level was measured by ELISA and leptin receptor density by using immuno-histochemistry. The soluble leptin receptor in group treated with 40% of normal daily calori diet was found significantly lower than control (p = 0.02). There were no any significant differences among group treated with 40 % of normal daily calori diet, “1 day fast-1day eat”, and ”1day fast-2 days eat” groups, and among 1 day fast-1 day eat”, ”day fast - 2 days eat” and control groups as well. On the other hand, leptin receptor density in adipose tissues was higher in restricted diet group than control. Diet of 40 % normal daily calorie for 4 weeks decreased soluble leptin receptor level, but increased adipocyte leptin receptor density of the adipose tissue of rat adventitial aorta. These changes may be resulted from an up regulation mechanism in relation with homeostatic maintenance. (Med J Indones 2006; 15:145-50) Keywords: restricted diet, leptin receptor, soluble leptin receptor, adipocyte, obesity
YLS-THERAPY: POTENSI SUPERNATAN YOGURT LBA–ST SEBAGAI ANTIKANKER SERVIKS BERBASIS BIOMOLEKULER Permata Sari, Desie Suci; Ridho, Wildan Mochamad; Lizziyyannida, Lizziyyannida; Sholihah, Zurniatus; Putra Pratama, Satya Hanggara; Rahmani, Erita; Riawan, Wibi
Program Kreativitas Mahasiswa - Penelitian PKM-P 2014
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

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Cervical cancer is the third most diagnosed cancer and the fourth leading cause of cancer death in females in the world. Zebrafish have been used as experimental animals in human tumors because of similar gene expression (70%) than other experimental animals. Yogurt LBA-ST produces SCFA metabolites (acetate, lactate, and butyrate) that can provide anticarcinogenic effects. There has been a vast growth of related research focusing on proliferation and apoptosis without clear mechanism. However, Important concern need to considered in the development of tumors is protein-coding invasion, migration, and adhesion (IMA) of tumor cells. This study aims at investigating (1) Antiproliferation activities of supernatan yogurt LBA-ST (SY LBA-ST) on zebrafish embryos and HeLa cells, and (2) anti-IMA’s pathway on HeLa cells. The anti-cancer property of SY LBA-ST was evaluated by MTT assay for viability and imunositochemistry of MMP-2, Laminin5-y2, Hsp27, and TGF-β for anti-IMA mechanism. The data is collected and analyzed using One-Way ANOVA.This study could be concluded that (1) SY LBA-ST increase mortality rate and decreased the hatching rate in zebrafish embryos, LC50 ≈ 20% (v/v),  (2) supernatant, pellet, and whole yogurt shows antiproliferation activity on HeLa cells, (3) IC50 SY LBA-ST ≈ 30% (v/v) and the ED = 10% (v/v), and (4) the anti-IMA’s pathway was via decrease MMP-2, Laminin5-y2, Hsp27, and TGF-β expression (p<0.05 ± 0.005).Keywords:  lactobacillus bulgaricus-acidophilus, streptococcus thermophillus, cancer, HeLa, zebrafish
PROFIL KADAR SOLUBLE UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR (suPAR) PADA SERUM PENDERITA TUBERKULOSIS PARU (SEBAGAI MONITORING TERAPI) Astuti, Triwahju; MR, Tri Yudani; Riawan, Wibi; Muktiati, Nunuk Sri; Widjajanto, Edy
Jurnal Kedokteran Brawijaya Vol 24, No 1 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.873 KB) | DOI: 10.21776/ub.jkb.2008.024.01.4

Abstract

Dutch study shows that Upar expression content is significantly higher in tubercolusis patient?s psiphsy?monosit compared to those in the healthy control group. So far, there is no biologic marker used whichcan?accurately observe response improvement in the treatment of lung tubercolusis. The aim of this research is to?investigate whether the serum level of soluble utokinase plasminogen activator receptor (suPAR) carries?information in monitoring TB treatment for Lung Tuberculosis patients. suPAR was measured by ELISA in 21?individuals at the time of enrolment into observational cross sectional based on active tuberculosis ?and 5?individuals as healthy control. There were 3 groups, 1). patients who had not started treatment (n=7),2).?patients who had been treated for 2 months (n=7), ?3). patients who had been treated for 6 months (n=7).?Among groups, there were no difference in mean of body mass index, erythrocyte sedimentation rate and?monocyte count. Patients positive for TB on direct ?microscopy were 29% ( 6 from 21 patients) , 2 patients?each groups. The suPAR levels were elevated in patients with active TB compared to healthy control?(P<0,001). suPAR levels were highest in patients positive for TB on direct microscopy ( mean suPAR 4.455?ng/ml).
PENGARUH PEMBERIAN OX-LDL TERHADAP AKTIVASI NF-кB DAN PPARγ γγ γSERTA APOPTOSIS PADA KULTUR HUVEC’s Riawan, Wibi; Wibowati, Samodijanti
Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (912.71 KB) | DOI: 10.21776/ub.jkb.2006.022.01.5

Abstract

Oxidized LDL (ox-LDL) causes activation and dysfunction of endothelial cells through NF?B activation after binding?process of ox-LDL to LOX-1. One of ox-LDL components had been known as the activator of PPAR?. This study was?done to see the effect of ox-LDL on NF?B and PPAR?activation and apoptosis of HUVEC?s culture. HUVEC?s culture?was divided into 3 groups : control (without any treatment) and 2 groups which were treated with ox-LDL 25ug/mL and?50ug/mL. Activation of NF?B and PPAR?were determined by immunodoublestaining using mouse monoclonal anti?NF?B and mouse monoclonal anti PPAR?after 30 minutes exposure of ox-LDL. The expression of TNF?and apoptotic?cell were determined by immunodoublestaining using ?TUNEL fragmented DNA labeling and goat polyclonal anti human?TNF?after 24 hours exposure of ox-LDL. HUVECs culture that had been treated with 25 ??g/ml and 50?g/ml of ox-LDL,?showing activation of NF?B but not PPAR?. This treated endotel showed apoptotic characteristic which had conformed?with DNA fragmented. These cells also showed the increase of TNF?expression on the cytoplasm. Ox-LDL could?increase translocation of NF?B gen transcription to nucleus followed by the increase of TNF?that can cause apoptotic?of the cells.?Key words: ox - LDL, Endothel, NF-?B, PPAR?, Apoptosis
ANALISIS LOSS OF HETEROZYGOSITYGEN RAR β2 PADA PATOGENESIS KANKER PAYUDARA Muliartha, I Ketut Gede; Riawan, Wibi; Hidayat, Asnah; Armania, Fitri
Jurnal Kedokteran Brawijaya Vol 24, No 2 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.671 KB) | DOI: 10.21776/ub.jkb.2008.024.02.5

Abstract

The aim of this research was to investigate the event of loss of heterozygosity of retinoid acid receptor (RAR β2) gene on the chromosom 3p24 in the pathogenesis ofbreast cancer. The breast cancer is the most malignant tumor that leads the second cause of death among the female. The recent concept of the theory of pathogenesis breast cancer is molecular genetic approaches such as apoptosis gene, oncogen, suppressorgene, and growth promoting factor gene. However, the diagnostic and therapy of breast cancer are still not satisfied. The lost or decreased in the expressionof RAR  β2gene on 3p24 chromosome via loss of heterozygosity (LOH) mechanism are the role of pathogenesis of breast cancer. Design of the research is by experimental and observational. Human female breastcancer and blood samples were gained from public hospital RSUD dr. Saiful Anwar, Malang. DNA was extracted from lymphocyte and tissue using Macherey-Nagel system kit. Loss of heterozygosity (LOH) incident in 3p24 chromosome was detected by PCR and Chromogenic in situ Hybridization (CISH) method.