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The Impact of Dissolved Nitrate and Phosphate on Maximum Growth Rate and Carrying Capacity of Oscillatoria in Intensive Shrimp (Litopenaeus vannamei) Farming Pond Situbondo, East Java, Indonesia Aliviyanti, Dian; Suharjono, Suharjono; Retnaningdyah, Catur
The Journal of Experimental Life Science Vol 7, No 1 (2017)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2016.007.01.11

Abstract

The aims of study are to analyze the effect of dissolved nitrate and phosphate content of the intensive shrimp farming pond Situbondo to maximum growth rate and carrying capacity of Oscillatoria population density in the laboratory.  This is an experimental research method using completely randomized design with three replications. The treatment were variation of nitrate and phosphate concentration (N0; N6; N12; N24; N48; P0,2; P0,4; P0.8, P1.6  mg.L-1).  Experiment was done using a pure Oscillatoria culture in condition 25 watt lamp; 12 hours a day.  The initial amount of Oscillatoria cells used for the treatment is 8 – 15 x 104 cell.mL-1.  During the incubation process, chemical parameters were also observed including nitrate, phosphate, DO, and pH at the beginning of the incubation period. Oscillatoria cell was count every day until stationary phase for 30 days.  Furthermore, a different test between treatments was conducted to determine levels of nitrate and phosphate in triggering the blooming of Oscillatoria using Oneway ANOVA analysis with SPSS Program.  The results showed that the intensive shrimp pond waters of Situbondo already contain dissolved phosphate between 0.4 - 0.5 mg.L-1.  Oscillatoria growth is strongly influenced by dissolved phosphate content in waters, phosphate levels of 0.2 - 0.4 or equivalent to the actual level of 0.6 - 0.7 mg.L-1 can cause the highest abundance of Oscillatoria that could endanger the ecosystem. Keywords: dissolved phosphate, intensive shrimp farming, Nitrate, Oscillatoria.
Karakteristik Vegetasi Riparian dan Interaksinya dengan Kualitas Air Mata Air Sumber Awan Serta Salurannya di Kecamatan Singosari Malang Rachmawati, Ekki Totilisa; Retnaningdyah, Catur
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

The aims of this research are to know the characteristics of riparian vegetation based on QBR (Qualitat del Bosc de Ribera) analysis and profile of water quality (pH, DO, temperature, conductivity, turbidity) as well as to know the interaction between biotic index of the riparian vegetation and water quality in Sumber Awan Spring and its channel. Observations of riparian vegetation and water quality were conducted at seven stations. The correlation between quality of riparian vegetation and water quality was determined by Pearson correlation analysis using SPSS v.16. The results of this study indicate that the quality of riparian vegetation in the spring (station one) was the best from all downstream stations. This is indicated by the highest value of QBR (60), species richness (27 species) and species diversity index (>3). Downstream from the spring, especially five and six stations (residential area) showed lowest riparian quality, indicated by decreasing of diversity and QBR index value.The pH and DO value had fulfilled the standard for class I and class II respectivelybased on Indonesia Governmental Regulation No. 82/2001. When shrubs and tree species are high, the low value of turbidity was occurs in the water. Therefore, when the QBR score was high, the conductivity value will low in the water. Then, when pH was low, the richness of shrubs, tree species, and QBR score will high. According to QBR and water quality, Sumber Awan ecosystem was degraded. Keywords : riparian vegetation, Sumber Awan spring water, water quality
Evaluation of water quality due to human activities in the Sumber Awan spring and its chanel, Singosari Malang Retnaningdyah, Catur
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

The aims of this research was to determine the profile of water quality based on physico-chemical properties of water, water quality index, and to determine the influence of various human activities on water quality in the Sumber Awan Spring and its Channel, Singosari Malang. Water quality evaluation is done in the spring and six locations of the spring channel after passing through agricultural activities, public toilets and human settlements with the distance of each location approximately 200 m. Physico-chemical parameters of water were observed include turbidity, pH, DO, permanganate, TSS, nitrate, ammonium, and BOD. The monitoring data is used to perform the analysis of water quality profiles using ANOVA, cluster analysis based on Bray-Curtis similarity index and biplot as well as water quality indices include Pratis implicit Index of Pollution. The results showed that human activities that occur in the channel of Sumber Awan such as agriculture, human settlements and public toilets have been affected the water quality in this spring channel. It can be seen from the decreasing of water quality from upstream to downstream. Values of pH, ammonium, permanganate, TSS and nitrate fulfilled the quality standards for class I, DO for class II, BOD for class II-IV, based on Government Regulation No. 82/2001 and turbidity value based on WHO only station one fulfilled the quality standards. Based on Prati’s index calculations the water quality was categorized in acceptable (station 1-6) and slightly polluted (station 7). Keywords : Malang, physico-chemical water quality, Sumber Awan
STRUKTUR KOMUNITAS MAKROINVERTEBRATA BENTOS DI SALURAN MATA AIR NYOLO DESA NGENEP KECAMATAN KARANGPLOSO KABUPATEN MALANG Rahmawati, Noviana Nur; Retnaningdyah, Catur
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

Penelitian ini bertujuan untuk mengetahui profil struktur komunitas makroinvertebrata bentos untuk evaluasi kualitas air di mata air Nyolo dan salurannya yang terletak di Desa Ngenep Kecamatan Karangploso Kabupaten Malang. Pengambilan sampel makroinvertebrata bentos dilakukan pada enam stasiun meliputi sekitar mata air Nyolo (stasiun satu dan dua), Curah Glogo (stasiun tiga dan empat), dan Curah Lang-lang (stasiun lima dan enam). Sampel diambil sebanyak ±100 individu/stasiun menggunakan Jaring Surber dan handnet. Pengumpulan data tiap stasiun meliputi komposisi dan kerapatan tiap spesies selanjutnya digunakan untuk menghitung kekayaan taksa, INP (Indeks Nilai Penting), dan indeks diversitas (H’). Hasil penelitian menunjukkan bahwa kekayaan taksa makroinvertebrata bentos yang ditemukan sebanyak 28 taksa dan bervariasi pada tiap stasiun. Baetidae, Caenidae, Melanoides tuberculata, Odonata dari famili Euphaeidae, Planaria dan Hydropsychidae jenis Cheumatopsyche sp. ditemukan di semua stasiun. Pada setiap stasiun didominasi oleh jenis yang berbeda, di stasiun satu terdapat  kodominasi Chironomidae dan Cheumatopsyche sp., stasiun dua didominasi oleh Melanoides tuberculata, stasiun tiga terdapat kodominasi Lymnaea rubiginosa, Thiara scabra, dan Dugesia trigina, stasiun empat terdapat kodominasi Cheumatopsyche sp. dan Simuliidae, dan di stasiun lima dan enam didominasi oleh Baetidae. Tingkat pencemaran bahan organik semakin menurun pada saluran yang semakin jauh dari mata air berdasarkan taksa makroinvertebrata bentos dominan yang ditemukan, namun demikian tingkat pencemaran bahan toksik  berdasarkan indeks diversitas Shannon-Wiener semakin tinggi. Kata kunci: makroinvertebrata bentos, mata air Nyolo dan salurannya, struktur komunitas
Bleach Solution Requirement for Hatching of Daphnia magna Resting Eggs Retnaningdyah, Catur; Ebert, Dieter
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.11594/jtls.06.03.01

Abstract

Daphnia (water fleas) belong to the zooplankton group called Cladocerans have sexual reproduction when conditions less favorable that produce diapausing eggs are enclosed in the ephippium. Hatching ephippial eggs in the laboratory is important in ecological, toxicology, genetical, and evolutionary studies. This study aims to improve the current methods of egg hatching from ephippium. Each of 50 ephippium were treated together by placing them in a glass jar and adding 50 mL bleach solution (sodium hypochlorite). Concentrations of sodium hypochlorite used in this experiment were 0%, 0.5%, 1%, 2%, 4% and 8%. These concentration treatments were crossed with the following exposure times (1, 2, 4, 8, 16, and 32 minutes). Culturing was done in 80 mL of artificial Daphnia medium, incubated in constant light and temperatures 20°C for 25 days. There were two repetitions in this experiment that were run at the same time. Result of this experiment showed that pretreatment with 0.5-8% bleach solution significantly increases the yield of total hatch rate of Daphnia magna resting eggs by about 21% over unbleached control. However, there was no significant difference among the bleach treatments. Concentration of bleach solution 0.5%, 1% and 4% significantly accelerated the time period until the first hatching (first day hatching). Difference of exposure time (1 - 32 minutes) at each concentration treatments were not influence the yield of total hatch and the time period until first hatching.
Geochemical Fraction Profile of Metal Cd, Pb and Zn on Sediments of Sidoarjo Mud Reclamation Area at Porong Sidoarjo Estuary Setiadi, Ahmad Dody; Rumhayati, Barlah; Retnaningdyah, Catur
Natural B Vol 4, No 1 (2017)
Publisher : Natural B

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.natural-b.2017.004.01.2

Abstract

Sediments of Porong River estuary suspected of containing a high concentration of heavy metals. the Sediment sample was collected from Porong Estuary river at two different location. The purposes of this study to determine the distribution concentration of metals geochemical fraction (Cd, Pb and Zn) at sediment Porong River estuary and correlation Against Physico-chemical properties of sediments. Analysis of sediment includes determining the concentration of Cd, Pb and Zn and determining of physicochemical properties of sediments such as pH, salinity, redox potential, cation exchange capacity, organic materials and sediment particles size. Extraction Method performed by BCR (Community Bureau of Reference) three steps sequential extraction, where a geochemical fraction of metals divided to 4 fractions, such acid soluble, reducible, oxidized and residual fractions. Research showed Zn has the highest concentration of each fraction and followed by Pb and Cd. Concentration of Zn in Fraction 1-4 ranged from 31,909 – 84,966 mg/Kg, Pb concentrations range 3,354 – 18,956 mg/Kg, whereas Cd concentrations range between 0,221 – 0,611 mg/Kg. The order of metals geochemical fraction concentration at Fraction 1, 2, 3 and 4 is Zn>Pb>Cd. There is some correlation between metals concentration against physicochemical properties of sediment. Where the physicochemical properties of sediment influence heavy metal content and concentration in the sediment of Porong River Sidoarjo.
Potensi Beberapa Hidromakrofita Lokal untuk Meningkatkan Kualitas Air Lindi Tempat Pemrosesan Akhir Sampah Talangagung, Kecamatan Kepanjen, Kabupaten Malang Khinanty, Retno Dewi; Retnaningdyah, Catur
Biotropika: Journal of Tropical Biology Vol 5, No 1 (2017)
Publisher : Biotropika: Journal of Tropical Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2016.005.01.1

Abstract

Tujuan penelitian ini adalah menentukan potensi hidromakrofita lokal sebagai agen fitoremediasi air lindi Tempat Pemrosesan Akhir (TPA) Sampah Talangagung Kecamatan Kepanjen Kabupaten Malang. Penelitian ini merupakan eksperimen dengan Rancangan Acak Lengkap (RAL) menggunakan lima perlakuan hidromakrofita lokal, yaitu kontrol (tanpa hidromakrofita), Ludwigia adscendens, Alternanthera sessilis, Typha angustifolia, dan polikultur ketiganya pada 25% air lindi dengan lima kaliulangan. Setiap perlakuan diinkubasi selama 30 hari di rumah kaca. Kualitas air lindi yang diamati adalah DO, turbiditas, nitrat, ortofosfat, dan BOD yang dipantau setiap enam hari sekali. Data tiap parameter dianalisis dengan uji beda antar waktu pantau pada masing-masing perlakuan. Semua hidromakrofita perlakuan mampu meningkatkan kualitas air lindi, yang ditandai dengan peningkatan nilai DO serta penurunan turbiditas, nitrat, ortofosfat, dan BOD. Hidromakrofita L. adscendens, T.angustifolia, dan polikultur (polikultur ketiga jenis tanaman) mampu menurunkan konsentrasi nitrat, ortofosfat, dan BOD sejak hari ke-12, sedangkan hidromakrofita A. sessilis sejak hari ke-18.Hidromakrofita A. sessilis mampu menurunkan 73,8% konsentrasi nitrat pada hari ke-30 dan 68,4% konsentrasi ortofosfat pada hari ke-24. Penurunan BOD (61,4%) terbesar ditemukan pada perlakuanhidromakrofita L. adscendens dan T. angustifolia hari ke-30
Perubahan Struktur Komunitas Makroinvertebrata Bentos Akibat Aktivitas Manusia di Saluran Mata Air Sumber Awan Kecamatan Singosari Kabupaten Malang Mariantika, Lina; Retnaningdyah, Catur
Biotropika: Journal of Tropical Biology Vol 2, No 5 (2014)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

Penelitian ini bertujuan untuk mengetahui perubahan struktur komunitas makroinvertebrata bentos dan kualitas air di saluran mata air Sumber Awan berdasarkan indeks biotik. Makroinvertebrata bentos diambil di tujuh titik pengambilan sampel masing-masing sebanyak ±100 individu, lalu diidentifikasi, dicari struktur komunitas dan nilai indeks HBI, FBI dan ASPT. Hasil penelitian menunjukkan bahwa struktur komunitas makroinvertebrata bentos pada stasiun dua hingga lima didominasi oleh jenis yang intoleran terhadap pencemaran yakni Hydropsychidae dan Lepidostomatidae, stasiun satu dan enam didominasi oleh jenis fakultatif yakni Thiaridae, dan stasiun tujuh didominasi oleh cacing Oligochaeta dan Chironomidae yang toleran terhadap pencemaran. Berdasarkan nilai FBI dan H, stasiun satu hingga enam digolongkan memiliki kualitas air sedang dan belum tercemar (nilai FBI 5,16-5,57 dan H 2,05-2,77), sedangkan stasiun tujuh digolongkan memiliki kualitas air yang sangat buruk dan tercemar (nilai FBI 7,63 dan H 1,72). Berdasarkan nilai HBI dan ASPT, stasiun satu hingga lima digolongkan memiliki kualitas air bagus/air bersih (nilai HBI 4,89-5,27 dan ASPT 7,45-6,27), stasiun enam digolongkan memiliki kualitas air sedang/tercemar sedang (nilai HBI 5,56 dan ASPT 6), dan stasiun tujuh digolongkan memiliki kualitas air buruk/tercemar berat (nilai HBI 7,60, dan ASPT 4). Berdasarkan hasil tersebut disimpulkan bahwa semakin ke hilir telah terjadi gradasi penurunan kualitas air pada saluran air hingga ±800 m dari mata air Sumber Awan karena pencemaran bahan organik dari aktivitas manusia di sekitarnya yakni MCK, residu pertanian dan peternakan.Kata kunci: indeks biotik, kualitas air, makroinvertebrata bentos, Sumber Awan
Peningkatan Kualitas Air Irigasi Akibat Penanaman Vegetasi Riparian dari Hidromakrofita Lokal selama 50 Hari Prasetyo, Hamdani Dwi; Retnaningdyah, Catur
Biotropika: Journal of Tropical Biology Vol 1, No 4 (2013)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

Air irigasi berperan penting dalam pengairan sawah. Air irigasi sering tercemari oleh limbah hasil aktivitas antropogenik. Hidromakrofita telah diketahui mampu berperan sebagai agen fitoremediasi kualitas air. Penelitian ini bertujuan untuk mengetahui potensi beberapa hidromakrofita lokal yang ditanam di tepi saluran irigasi selama 50 hari sebagai vegetasi riparian dalam peningkatan kualitas air irigasi. Hidromakrofita lokal yang digunakan adalah Limnocharis flava, Ipomoea aquatica, Fimbristylis globulosa, Vetiveria zizanoides, Equisetum ramosissium, Typha angustifolia, Sesbania grandiflora dan Scirpus grossus. Vegetasi riparian ditanam sepanjang 275 m di saluran irigasi Desa Kedung Pedaringan Kecamatan Kepanjen Kabupaten Malang. Kualitas fisikokimia air meliputi TDS, Nilai KMnO4, Ortofosfat, dan Amonium diamati setelah penanaman selama 50 hari. Pemantauan dilakukan pada lokasi sebelum penanaman (hulu), setelah penanaman sepanjang 125 m (tengah) dan setelah penanaman sepanjang 275 m (hilir). Perbedaan nilai tiap parameter antar lokasi diketahui dari uji Anova, analisis Cluster dan analisis Biplot. Hasil penelitian menunjukkan bahwa adanya penanaman delapan jenis tanaman vegetasi riparian di sepanjang tepi saluran irigasi tersier mampu meningkatkan kualitas air irigasi. Penananaman tersebut mampu menurunkan kadar Nilai KMnO4, TDS, ortofosfat, dan ammonium secara signifikan. Penanaman vegetasi riparian sepanjang 275 m lebih efektif meningkatkan kualitas air dibandingkan penanaman sepanjang 125 m. Kata kunci:  Air irigasi, kualitas air, vegetasi riparian
STUDI KELAYAKAN KUALITAS AIR MINUM DELAPAN MATA AIR DI KECAMATAN KARANGPLOSO KABUPATEN MALANG Rahmawati, Rani; Retnaningdyah, Catur
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

Tujuan penelitian ini untuk melakukan evaluasi terhadap kualitas delapan mata air berdasarkan sifat fisik dan kimia air. Penelitian dilakukan di mata air Ngenep, PraNyolo, Umbulan, Langgar, Balittas, Lowoksari, Leses dan Soko yang terletak di Kecamatan Karangploso Kabupaten Malang. Pada  masing–masing  mata air  tersebut  dilakukan  pengambilan sampel air  pada dua titik sebagai ulangan. Parameter  yang diukur meliputi suhu, pH, DO, konduktivitas, turbiditas, BOD, nitrat, ortofosfat, alkalinitas, TDS, TSS, TOM dan debit. Perbedaan kualitas fisikokimia air antar mata air ditentukan dengan uji Anova yang dilanjutkan dengan Duncan Multiple Range test, analisis cluster dan PCA. Hasil penelitian menunjukkan bahwa delapan mata air yang dipantau sudah tidak layak digunakan sebagai bahan baku air minum. Parameter yang sudah tidak memenuhi  baku mutu  berdasarkan  PP No 82 tahun 2001 tentang  Pengelolaan  Kualitas  Air dan  Pengendalian Pencemaran Air adalah nilai DO (semua mata air), BOD (mata air  PraNyolo), Nitrat (mata air  Langgar, Balittas, Lowoksari, Leses, Soko).   Kata kunci: Indeks Kualitas air Prati’s, Karangploso, kualitas air,  mata air