Articles

Found 4 Documents
Search
Journal : Jurnal Kedokteran Maranatha

Perbandingan Pengaruh Ozon, Getah Jarak Cina (Jatropha Multifida L.) dan Povidone Iodine 10% terhadap Waktu Penyembuhan Luka pada Mencit Betina Galur Swiss Webster Dewiyanti, Alvina; Ratnawati, Hana; Puradisastra, Sugiarto
Jurnal Kedokteran Maranatha Vol 8, No 2 (2009)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Unlike the popular povidone iodine 10% and the effective Jatropha multifida L. latex, ozone has been used as an alternative wound healing therapy for a long time but the advantage is still controversial. The aim of this research is to compare the effect of ozone, Jatropha moultifida  L. latex and povidone iodine 10% on the wound healing process. The experiment used a Random Complete Design with comparative characteristics. The animal models were 24 adult Swiss Webster female mice, divided into 4 groups (n=6). The thighs of the mice were shaved before an 8-mm slice on the skin was made.  Group I had no treatment, group II was treated with povidone iodine 10% topically, group III with ozone and and group IV with  Jatropha multifida L. latex. The treatment and the wound length measurements were carried out every day until all the wounds were healed. The result showed that the average time span of wound healing of each of groups I, II, III, and IV was subsequently 6.80 days, 6.88 days, 4.17 days, and 5.83 days. The time span of the healing process in the 3rd (ozone) group was very significantly  different from that of the negative control group (p=0.001) and it was significantly different from that of Jatropha multifida L. latex group (p=0.0032). The time spans of the healing process of group II (povidone iodine 10%) and group IV (Jatropha multifida L. latex) were not significantly different from that of the negative control group (p=0.364). The conclusion is that ozone significantly accelerates the wound healing process more than Jatropha multifida L. latex and povidone iodine 10%.
Pengaruh Pemberian Ekstrak Buah Merah (Pandanus conoideus Lam.) terhadap Aktivitas Fagositosis Makrofag Ratnawati, Hana; Handoko, Yudhi; Purba, Leonard H.
Jurnal Kedokteran Maranatha Vol 7, No 1 (2007)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The immune system has an important role in human health. The innate immune-system defends the host against infection by phagocytes the antigen, which is the role of macrophage cell.  Red Fruit (Pandanus conoideus Lam.) had been empirically proved to increase body immune system. The aim of this study is to know the effect of Red Fruit toward the macrophage’s phagocytotic activity. This research was acted invitro on Swiss Webster mice’s macrophage cell culture, which was exposured by Red Fruit extract (concentration 0,25ug/dl; 0,50ug/dl; 1,00ug/dl; 2,50ug/dl; 5,00 ug/dL) in 5’, 10’, 30’, and 60’. The percentage of activated macrophages was counted, compared between treatment group and negative control group (aquadest). The data was processed by One Way Anova and Tukey HSD statistical methods. The result shows that 10’, 30’ and 60’ exposured of Red Fruit extract could increase Macrophage’s activity significantly (p=0,000). The optimal dose of Red Fruit extract which could increase the activity of macrophage is 0,25 µg/dl. With 10’, 30’ and 60’ exposured of Red Fruit could increase the phagocytotic activity of macrophages 76.15%  ug/dl, 65.32% ug/dl, and 83.41%  ug/dl. It can be concluded that the exposurd of Red Fruit extract 10’, 30’ and 60’ could increase macrophage’s phagocytotic activity, with the optimal dose is 0,25 µg/dl.
Pengaruh pH terhadap Pertumbuhan Salmonella typhi In Vitro ., Hanna; Tyasrini, Endah; Ratnawati, Hana
Jurnal Kedokteran Maranatha Vol 5, No 1 (2005)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Salmonella typhi merupakan salah satu penyebab penyakit infeksi tersering di daerah tropis. Manusia terinfeksi Salmonella typhi secara fecal-oral, biasanya melalui makanan yang kurang higienis dan atau kurang masak. Salmonella typhi pertama-tama menginfeksi Peyer’s patches pada ileum dan untuk mencapai ileum Salmonella typhi harus melalui gaster yang mempunyai pH antara 1,4 – 1,6. Pada umumnya, Salmonella typhi akan mati pada pH gaster yang rendah tersebut.Penelitian ini bersifat eksperimental dan bertujuan untuk mengetahui pH minimum di mana Salmonella typhi dapat hidup dan mengetahui pengaruh pH terhadap pertumbuhan Salmonella typhi. Suspensi Salmonella typhi berumur 18 – 24 jam dengan pengenceran 1/1.000.000 ditanamkan pada medium SS agar dengan pH 2,5 – 8 menggunakan metoda streak plate, kemudian diinkubasi selama 24 jam. pH medium diatur dengan menambahkan HCl pekat atau NaOH 2N ke dalam SS agar cair. Jumlah kuman yang tumbuh dihitung dalam colony forming unit (cfu) dikalikan faktor pengenceran, diambil rata-ratanya, kemudian dibandingkan dengan rata-rata jumlah kuman pada kontrol positif.Hasil penelitian menunjukkan tidak ada Salmonella typhi yang tumbuh pada medium dengan pH 2,5 dan 3. Rata-rata jumlah kuman pada medium dengan pH 3,5 adalah 30.333.333, pH 4 = 35.000.000, pH 5 = 44.666.667, pH 6 = 75.666.667, pH 7 = 71.000.000 dan pH 8 = 66.000.000. Rata-rata jumlah kuman pada medium kontrol positif adalah 50.666.667.Disimpulkan bahwa Salmonella typhi mulai dapat tumbuh pada pH 3,5 dan pertumbuhannya menunjukkan peningkatan dari pH 3,5–6 serta tumbuh optimal pada pH 6–8.
Leaky Gut sebagai Penyebab Gangguan Gastrointestinal pada ASD Ratnawati, Hana
Jurnal Kedokteran Maranatha Vol 2, No 2 (2003)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The increased of intestinal permeability, which is known as leaky gut is key to many theories of autism. The intestinal permeability plays a critical role in helping the body to adequatly absorb nutrients and to block toxins, bacteria, various allergens, and other potentially harmful molecules penetrating into the sistemic circulation.The abnormal structures in the brains of children with autism may be due to the toxic effects of the microbial metabolites or the abnormal peptides from wheat and milk. These harmful molecules could be transported across tight junctions between epithelial cells (paracellular route) due to the hyperpermeability of the intestine mucous and the damage of the mucous layer intestine (leaky gut). The damage of the intestinal mucous layer caused by a yeast overgrowth of the gastrointestinal tract and the yeast could produce enzymes which digest the lining of the intestinal tract itself. The microbial metabolites byproducts and undigested wheat products absorbed from the intestinal tract into the body and elicit an allergic response, nutritional deficiencies of key nutrients and may manifest as behavioral disorders if it broke through the blood brain barrier.Leaky gut usually provoked by exposure to substances which damage the integrity of the intestinal mucosa, such as infectious agents (bacteri, virus, fungi), antibiotic, NSAID, fungalmycotoxins, food dyes and cytotoxic drugs.