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Phytochemical Assay and Antiplatelet Activity of Fractions of Velvet Bean Seeds (Mucuna pruriens L.) WIDOWATI, WAHYU; RATNAWATI, HANA; RUSDI, UDJU DJUNAEDI; WINARNO, WAHYU; IMMANUEL, VICTOR
HAYATI Journal of Biosciences Vol 17, No 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.207 KB) | DOI: 10.4308/hjb.17.2.85

Abstract

Platelet aggregation is an important factor contributing to the formation of thrombus due to an uncontrolled blood clotting. An antiplatelet agent is a compound which decreases platelet aggregation and inhibits thrombus formation. The objectives of this study were to determine the class of compound employing phytochemical assay and to determine the in vitro antiplatelet activity of four fraction, namely hexane, ethyl acetate, butanol, and water fractions of velvet bean seeds (Mucuna pruriens L.) using epinephrine (EPN) as agonist of platelet aggregation. The antiplatelet activities were tested in human platelet rich plasma with hyperaggregation. To determine the activities, EPN was arranged at 4 level of concentrations  (300, 150, 75, and 30 μM), and antiplatelet agents were at 500 µg/ml. The results indicated that ethyl acetate, butanol and water fraction contained high flavonoids and moderate phenols. The water, butanol and ethyl acetate fractions of velvet bean seeds exhibited potential inhibition of EPN-induced platelet aggregation at all concentrations. The strongest antiplatelet agent was water fraction and had the same antiplatelet activity as aspirin at level 150, 75, and 30 μM of EPN. Butanol fraction had the same antiplatelet activity as aspirin at the lowest EPN (30 μM).
The Role of of Red Fruit (Pandanus conoideus Lam) Oil towards Colitis Ulcerative Mice Model Khiong, Khie; Soeng, Sylvia; Ratnawati, Hana
Journal of the Indonesian Medical Association Vol. 62 No. 4 April 2012
Publisher : Journal of the Indonesian Medical Association

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Abstract

Introduction: Ulcerative colitis (UC) is chronic inflammation process in gut due to dysregulationof mucous immune systems. Oxidative stress caused by free radicals is believed to aggravate the degree of the disease. Red fruit (Pandanus conoideus Lam.) is indigenous plant from Papua that contains high level of antioxidants. The aim of this research were to examine the role of red fruit oil towards leukocytes proliferation and hystological feature of colon in colitis mice modelMethod: Colitis induced group were divides into 2 gropus; with and without red fruit oil treatment. Red fruit treated groups were given red fruit oil in various dosages of 0,1 ml; 0,2 ml and 0,4 ml respectively. At the end of experiment, all mice were sacrificed and the colons were removed and subjected to histopathological analysis by staining with HE. Lymphocytes were measured using MTT assay method.Result: From histopathological analysis results, 0,1 ml -red fruit oil treated group showed improving colon structure compared to untreated group. Red fruit oil of 0.1 ml and 0.4 ml groups showed increased of non-T leukocytes proliferation significantly compared to untreated group.Conclusion: Red Fruit extract can increase non-T leukocytes and T lymphocytes proliferationsignificantly. J Indon Med Assoc. 2012;62:142-8.Keywords: Red fruit extract, leukocytes and lymphocytes proliferation, Dextran Sulfate Sodium (DSS)
Perbandingan Pengaruh Ozon, Getah Jarak Cina (Jatropha Multifida L.) dan Povidone Iodine 10% terhadap Waktu Penyembuhan Luka pada Mencit Betina Galur Swiss Webster Dewiyanti, Alvina; Ratnawati, Hana; Puradisastra, Sugiarto
Jurnal Kedokteran Maranatha Vol 8, No 2 (2009)
Publisher : Universitas Kristen Maranatha

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Abstract

Unlike the popular povidone iodine 10% and the effective Jatropha multifida L. latex, ozone has been used as an alternative wound healing therapy for a long time but the advantage is still controversial. The aim of this research is to compare the effect of ozone, Jatropha moultifida  L. latex and povidone iodine 10% on the wound healing process. The experiment used a Random Complete Design with comparative characteristics. The animal models were 24 adult Swiss Webster female mice, divided into 4 groups (n=6). The thighs of the mice were shaved before an 8-mm slice on the skin was made.  Group I had no treatment, group II was treated with povidone iodine 10% topically, group III with ozone and and group IV with  Jatropha multifida L. latex. The treatment and the wound length measurements were carried out every day until all the wounds were healed. The result showed that the average time span of wound healing of each of groups I, II, III, and IV was subsequently 6.80 days, 6.88 days, 4.17 days, and 5.83 days. The time span of the healing process in the 3rd (ozone) group was very significantly  different from that of the negative control group (p=0.001) and it was significantly different from that of Jatropha multifida L. latex group (p=0.0032). The time spans of the healing process of group II (povidone iodine 10%) and group IV (Jatropha multifida L. latex) were not significantly different from that of the negative control group (p=0.364). The conclusion is that ozone significantly accelerates the wound healing process more than Jatropha multifida L. latex and povidone iodine 10%.
Pengaruh Pemberian Ekstrak Buah Merah (Pandanus conoideus Lam.) terhadap Aktivitas Fagositosis Makrofag Ratnawati, Hana; Handoko, Yudhi; Purba, Leonard H.
Jurnal Kedokteran Maranatha Vol 7, No 1 (2007)
Publisher : Universitas Kristen Maranatha

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Abstract

The immune system has an important role in human health. The innate immune-system defends the host against infection by phagocytes the antigen, which is the role of macrophage cell.  Red Fruit (Pandanus conoideus Lam.) had been empirically proved to increase body immune system. The aim of this study is to know the effect of Red Fruit toward the macrophage’s phagocytotic activity. This research was acted invitro on Swiss Webster mice’s macrophage cell culture, which was exposured by Red Fruit extract (concentration 0,25ug/dl; 0,50ug/dl; 1,00ug/dl; 2,50ug/dl; 5,00 ug/dL) in 5’, 10’, 30’, and 60’. The percentage of activated macrophages was counted, compared between treatment group and negative control group (aquadest). The data was processed by One Way Anova and Tukey HSD statistical methods. The result shows that 10’, 30’ and 60’ exposured of Red Fruit extract could increase Macrophage’s activity significantly (p=0,000). The optimal dose of Red Fruit extract which could increase the activity of macrophage is 0,25 µg/dl. With 10’, 30’ and 60’ exposured of Red Fruit could increase the phagocytotic activity of macrophages 76.15%  ug/dl, 65.32% ug/dl, and 83.41%  ug/dl. It can be concluded that the exposurd of Red Fruit extract 10’, 30’ and 60’ could increase macrophage’s phagocytotic activity, with the optimal dose is 0,25 µg/dl.
Pengaruh pH terhadap Pertumbuhan Salmonella typhi In Vitro ., Hanna; Tyasrini, Endah; Ratnawati, Hana
Jurnal Kedokteran Maranatha Vol 5, No 1 (2005)
Publisher : Universitas Kristen Maranatha

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Abstract

Salmonella typhi merupakan salah satu penyebab penyakit infeksi tersering di daerah tropis. Manusia terinfeksi Salmonella typhi secara fecal-oral, biasanya melalui makanan yang kurang higienis dan atau kurang masak. Salmonella typhi pertama-tama menginfeksi Peyer’s patches pada ileum dan untuk mencapai ileum Salmonella typhi harus melalui gaster yang mempunyai pH antara 1,4 – 1,6. Pada umumnya, Salmonella typhi akan mati pada pH gaster yang rendah tersebut.Penelitian ini bersifat eksperimental dan bertujuan untuk mengetahui pH minimum di mana Salmonella typhi dapat hidup dan mengetahui pengaruh pH terhadap pertumbuhan Salmonella typhi. Suspensi Salmonella typhi berumur 18 – 24 jam dengan pengenceran 1/1.000.000 ditanamkan pada medium SS agar dengan pH 2,5 – 8 menggunakan metoda streak plate, kemudian diinkubasi selama 24 jam. pH medium diatur dengan menambahkan HCl pekat atau NaOH 2N ke dalam SS agar cair. Jumlah kuman yang tumbuh dihitung dalam colony forming unit (cfu) dikalikan faktor pengenceran, diambil rata-ratanya, kemudian dibandingkan dengan rata-rata jumlah kuman pada kontrol positif.Hasil penelitian menunjukkan tidak ada Salmonella typhi yang tumbuh pada medium dengan pH 2,5 dan 3. Rata-rata jumlah kuman pada medium dengan pH 3,5 adalah 30.333.333, pH 4 = 35.000.000, pH 5 = 44.666.667, pH 6 = 75.666.667, pH 7 = 71.000.000 dan pH 8 = 66.000.000. Rata-rata jumlah kuman pada medium kontrol positif adalah 50.666.667.Disimpulkan bahwa Salmonella typhi mulai dapat tumbuh pada pH 3,5 dan pertumbuhannya menunjukkan peningkatan dari pH 3,5–6 serta tumbuh optimal pada pH 6–8.
Leaky Gut sebagai Penyebab Gangguan Gastrointestinal pada ASD Ratnawati, Hana
Jurnal Kedokteran Maranatha Vol 2, No 2 (2003)
Publisher : Universitas Kristen Maranatha

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Abstract

The increased of intestinal permeability, which is known as leaky gut is key to many theories of autism. The intestinal permeability plays a critical role in helping the body to adequatly absorb nutrients and to block toxins, bacteria, various allergens, and other potentially harmful molecules penetrating into the sistemic circulation.The abnormal structures in the brains of children with autism may be due to the toxic effects of the microbial metabolites or the abnormal peptides from wheat and milk. These harmful molecules could be transported across tight junctions between epithelial cells (paracellular route) due to the hyperpermeability of the intestine mucous and the damage of the mucous layer intestine (leaky gut). The damage of the intestinal mucous layer caused by a yeast overgrowth of the gastrointestinal tract and the yeast could produce enzymes which digest the lining of the intestinal tract itself. The microbial metabolites byproducts and undigested wheat products absorbed from the intestinal tract into the body and elicit an allergic response, nutritional deficiencies of key nutrients and may manifest as behavioral disorders if it broke through the blood brain barrier.Leaky gut usually provoked by exposure to substances which damage the integrity of the intestinal mucosa, such as infectious agents (bacteri, virus, fungi), antibiotic, NSAID, fungalmycotoxins, food dyes and cytotoxic drugs.
EFFECT OF ETHANOL EXTRACT SAMBILOTO LEAF (Andrographidis folium) AGAINST BLOOD GLUCOSE LEVEL IN MALE Swiss Webster MICE INDUCED BY ALOXAN COMPARED TO JAMU D -, Rosnaeni; Ratnawati, Hana; Kastilani, Sarah
Jurnal Medika Planta Vol 1, No 1 (2010)
Publisher : Jurnal Medika Planta

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Abstract

 Introduction: Jamu D is Indonesian traditional medicine containing ethanol extract of sambiloto leaf (EESL) which can be used as diabetes mellitus alternative drug. The aim of this study is to know the effect of EESL in lowering blood glucose levels comparde to Jamu D. Methods :The real prospective experimental comparative with complete randomized design was done. Twenty four aloxan induced diabetic male Swiss Webster mice was divided into 6 groups of treatment i.e. EESL dose 1 (29.25 mg/ kgBW), EESL dose 2 (58.5 mg/ kgBW), EESL dose 3 (117 mg/ kgBW),  control (CMC 1% suspension), comparator (Glibenklamid 0.65 mg/ kgBW), Jamu D (195 mg/ kgBW) for 7 days. The fasting blood glucose level was examined at the eight day and blood glucose level decrease percentage was analyzed with ANOVA, continued with Tukey HSD, a = 0.05. Results: showed that the decrease of blood glucose level in EESL dose1(65.93 %), dose (66.66 %), was same with Jamu D (59.82 %)(p >0,05) while the decrease percentage in dose 2 was significant different with Jamu D (p < 0,01). Conclusions :  EESL dose 1 and dose 3 were as effective as Jamu D in decreasing blood glucose level while dose 2 was not effective 
EFFECT OF ETHYL ACETATE AND BUTANOL FRACTIONS OF VELVET BEAN SEED (Mucuna Pruriens L.) TO IMPROVE LIPID PROFILE IN DYSLIPIDEMIC RATS Ratnawati, Hana; Widowati, Wahyu; Chakravitha, Clarissa
Jurnal Medika Planta Vol 1, No 1 (2010)
Publisher : Jurnal Medika Planta

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Abstract

Introduction: Nowadays many people had dyslipidemic, affected by dietery habits and unhealthy lifestyle. High blood lipid can increase the risk of atherosclerosis and coronary heart disease (CHD). The CHD mortality rate are increasing and known as first leading cause of death in Indonesia. Objective: to find out the effect of ethyl acetate and butanol fractions of velvet bean seed (M. pruriens L.) to improve lipid profile in dyslipidemic rats. Methods: laboratory experimental prospective design, in vivo  preclinical test used dyslipidemic rats, which given ethyl acetate and butanol fractions in concentration at 15, 30, 60mg/kgBW/day, simvastatine 27mg/kgBW/day during ten days. The parameters used respectively are HDL, LDL, triglyceride, and total cholesterol level on dyslipidemic rats. Data were  analysed  with  Anava  and continued Post Hoc Duncan with level of significancy  95%. Results : Ethyl acetate fraction 15mg/kgBW/day significantly decreased total cholesterol level (56.667mg/dL) compared to positive control (70.667mg/dL), and ethyl acetate fraction 60mg/kgBW/day significantly decreased LDL level (7.333mg/dL) compared to positive control (14.333mg/dL), butanol fraction 60mg/kgBW/day significantly decreased  triglyceride level (65.667mg/dL) compared to positive control (112.333mg/dL) and significantly  increased HDL level (30.333g/dL) compared to positive control (24.333g/dL). Conclusion:  Ethyl acetate and butanol fractions of M. pruriens L. improve lipid profile such as decrease LDL, triglyceride, total cholesterol level and increase HDL level in dyslipidemic rats.
The Comparison Effect of Aloe vera L., Psidium guajava Linn, Curcuma domestica Val to Colitis Ulcerative Mice Models Histopatology Kurniawati, Dewi; Jasaputra, Diana Krisanti; Ratnawati, Hana; Tiono, Hartini; Sujatno, Muchtan; Dewi, Aprilin Krista; Mayestica S., Putu; Arifin, Samuel
Jurnal Medika Planta Vol 1, No 2 (2010)
Publisher : Jurnal Medika Planta

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Abstract

Introduction: Ulcerative colitis (UC) is an inflammatory disease limited to the mucosal and submucosal layers of the colon. UC treatments was aimed to decrease inflammation and oxidation process on colon. Nowadays people start using hermal medicine to attentuate inflammation and oxidation process in ulcerative colitis, e.g. Aloe vera, Psidium guajava Linn, Curcuma domestica Val.Objective: to compare the effect of Aloe vera ethanol extract, Psidium guajava Linn ethanol extract, Curcuma domestica Val ethanol extract on histopatology of ulcerative colitis.Methods: experimental comparative research with completely randomized design. Mice were divided on 5 groups (n=6) which were given Aloe vera ethanol extract 700 mg/kgBB and DSS 2,5%; Psidium guajava Linn ethanol extract 1.591,2 mg/kgBB and DSS 2,5%; Curcuma domestica Val ethanol extract 180.816 mg/kgBB and DSS 2,5%; aquadest and DSS 2,5% (positive control); aquadest (negative control). Data were analyze with ANOVA followed by Tukey HSD test with a=0.05.Results: there were a decrement percentage of crypt loss on Aloe vera ethanol extract group (10.5 +- 4,64681%), Psidium guajava Linn ethanol extract group (11 +- 2,09762%), Curcuma domestica Val ethanol extract group (9 +- 3,09839%) compared with positive control group (26.8 +- 1,72240%)(p<0.05). Aloe vera ethanol extract, Psidium guajava Linn ethanol extract, Curcuma domestica Val ethanol extract had same effectivity (p>0.05).Conclusion: Aloe vera ethanol extract, Psidium guajava Linn ethanol extract, Curcuma domestica Val ethanol extract had same effectivity on decreasing percentage of crypt loss on colitis ulcerative histopatology of mice induced by DSS 2.5%.
Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line Widowati, Wahyu; Mozef, Tjandrawati; Risdian, Chandra; Ratnawati, Hana; Tjahyani, Susy; Sandra, Ferry
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Tapak dara or Madagascar Periwinkle (Catharanthus roseus [L] G.Don), a natural plant, is empirically reported to have promising anticancer activity. To elucidate its mechanism, a research was conducted to investigate the possible ethanol extract of  C.  roseus in inducing apoptosis on breast cancer cell line (T47D). Antioxidant activity of C. roseus was investigated as well. Sub-G1 flowcytometric apoptotic analysis result showed that extract of C. roseus at 6.25 μg/mL induced apoptosis for 26.365%. Increasing extract concentration resulted an increasing apoptotic level as well, extract at concentration of 12.5 μg/mL induced apoptosis for 22.235%.  Meanwhile doxorubicin at concentration of 10  μg/mL induced apoptosis for 36.055%. The antioxidant activity was determined by using  in vitro assay: inhibition of  2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging  activity. Antioxidant activity of  C. roseus extract were compared to quercetin and butylated hydroxyanisol (BHA), as positive controls.  The results showed that DPPH IC50 of C. roseus extract, quercetin and BHA were 358.411 μg/mL, 19.200 μg/mL  and  94.178  μg/mL, respectively. We suggest that  C. roseus extract had a potential anticancer activity by inducing apoptosis.