FEDIK ABDUL RANTAM
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga

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Analyses of Precore and Core Promoter Mutations of Hepatitis B Virus in Patients with Chronic Hepatitis B in Surabaya, Indonesia JUNIASTUTI, .; AKSONO, EDUARDUS BIMO; UTSUMI, TAKAKO; YANO, YOSHIHIKO; SOETJIPTO, .; HAYASHI, YOSHITAKE; HOTTA, HAK; RANTAM, FEDIK ABDUL; KUSUMOBROTO, HERNOMO ONTOSENO; LUSIDA, MARIA INGE
Microbiology Indonesia Vol 4, No 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

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Mutations of precore (A1896) and core promoter (T1762/A1764) of hepatitis B virus can reduce HBeAg production. These mutations are frequently found in the late HBeAg seroconversion. However, it has been a controversy about the role played by precore and core promoter mutations in determining outcome of chronic hepatitis B. In the present study, the variability of precore and core promoter of hepatitis B virus were analyzed using PCR amplification and sequencing, according to the outcome (viral load and HBeAg/anti-HBe) in chronic hepatitis B patients in Surabaya. The study groups included 5 patients with uncomplicated chronic hepatitis B and 10 patients with chronic hepatitis B and liver cirrhosis in Dr. Soetomo Hospital, Surabaya. The control group included 6 blood donors obtained from Indonesia Red Cross, Surabaya. All groups were HBsAg positive. Precore mutation A1896 was predominant in all groups (60%-67% of each), together with precore variant T1858. As reported, precore variant T1858 is a prerequisite for precore A1896 and characteristic for viral genotype. Nevertheless, core promoter mutations T1762/A1764 were predominant only in LC patients (60%). All of these mutations were found mostly after HBeAg seroconversion (anti-HBe+). Of most samples with anti-HBe+, precore mutation was related with low viral load (<105 copies/mL), but core promoter mutations with high viral load (>105 copies/mL). Precore mutation A1896 was predominant in all groups, but core promoter mutations T1762/A1764 were only predominant in LC patients. The precore mutation alone is possible not critical to indicate a poor outcome, the core promoter mutations must be considered also.
The Relation Between Levels of TNF Alpha, IL-1B, PGE2 and PLA with the Severity Degree of Dengue Hemorrhagic PURWATI, .; NASRONUDIN, .; KUSUMOWIDAGDO, ENDANG RETNOWATI; RANTAM, FEDIK ABDUL
Microbiology Indonesia Vol 5, No 2 (2011): June 2011
Publisher : Indonesian Society for microbiology

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The pathogenesis of dengue virus infection is still being debated.  Based on the existing data, there is a strong evidence that the immunopathological mechanism plays a role in dengue virus infection with various complications.  Some unknown immune responses play a role in the pathogenesis of dengue virus infection. Researchers are trying to establish the role of several inflammatory mediators such as PLA2, IL-1, TNF-, PGE2, PGI2, Thromboxane A2, Leucotrien and MPTP, in relation to the severity degree of the dengue virus infection.  The aim of this study is to recognize the relation between the severity degrees of DHF (Dengue Hemorrhagic Fever) patients and the immunological profile in the sub-cellular level, such as PLA2, IL-1, TNF-, PGE2 and PLA 2.  The collected data was processed and presented analytically. The relation between each parameter (TNFα, SPLA2, PGE2,IL-1β) and the degree of DHF was analyzes, using Spearman´s correlation analysis, ordinal regression, and using ANOVA.  It was shown that there was no relation between the levels of TNF-ά, PGE2, IL-1β, and SPLA2  in patients with various degrees of DHF, but there were significant differences between DHF grade 1 and 3, and also 2 and 3, on IL-1β.  There were increased levels of the four parameters in dengue grade 1 to 2, but decreased levels in grade 3. This can be caused by inflammatory processes, but the severity degree of DHF can also be influenced by complement, thromboxane, and leukotrien.
Antibody of goat zona pellucida-3 (gzp3) protein of mice(Mus musculus) block in vitro fertilization of mice as an animal model= Antibodi protein zona pelusida-3 kambing (gZP3) asal mencit(Mus musculus) mencegah ... Mustofa, Imam; Mahaputra, Laba; Dachlan, Yoes Priyatna; Rantam, Fedik Abdul; ., Suwarno; ., Widjiati; Hinting, Aucky
Jurnal Sain Veteriner Vol 24, No 1 (2006)
Publisher : Fakultas Kedokteran Hewan

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The researchs of immunocontraception have done in ZP3 of several species, but have not been done in ZP3 of goat. In preliminary study, gZP3 protein was effective prohibited of graviditation of mice. The aim of this study was to prove the potency of gZP3 protein to prohibit in vitro fertilization of mice as an animal model. Antibody of gZP3 produced on mice. Immunized mice serum was analyzed using Elisa and Dot blotting method. Antibody of gZP3 supplemented into M-16 media for oocyte incubation, continued with in vitro fertilization. The result showed that antibody titer of immunized mice serum was higher (p
Determinan Antigen Gen omp2a Brucella abortus Isolat Lokal Ratnasari, Ratih; Handijatno, Didik; Suwarno, .; Rantam, Fedik Abdul
ACTA VETERINARIA INDONESIANA - Indonesian Veterinary Journal Vol 2, No 1 (2014): Januari 2014
Publisher : Bogor Agricultural University

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Penyakit Brucellosis pada sapi disebabkan oleh Brucella abortus dan dikenal sebagai penyakitreproduksi menular pada ternak. Brucellosis merupakan penyakit zoonosis karena dapat menularpada manusia. Penelitian ini bertujuan untuk mengetahui determinan antigen yang terdapat pada genomp2a B. abortus isolat lokal yang telah diblasting ke asam amino (protein). Sampel bakteri berasaldari sapi penderita Brucellosis asal Sulawesi Selatan dan Nusa Tenggara Timur. Gen omp2a diamplifikasimelalui tehnik Polymerase Chain Reaction (PCR) dengan menggunakan primer 2ab5F dan 2a900R.Produk PCR disekuensing untuk mendapatkan sekuen nukleotida gen omp2a B. abortus isolat lokal.Sekuen nukleotida ini dianalisis tingkat homologinya terhadap isolat asal mancanegara yang diaksesdari GenBank dengan menggunakan BLAST. Sekuen nukleotida gen omp2a diblasting ke asam aminokemudian dengan metode Kolaskar dan Tongaonkar antigenicity dapat diperoleh determinan antigenpada antigen protein membran luar (OMP) B. abortus isolat lokal. Hasil menunjukkan bahwa tingkathomologi antara sekuen gen omp2a B. abortus isolat lokal dengan isolat asal mancanegara mempunyaitingkat homologi yang tinggi (99% - 100%). Hasil prediksi determinan antigen didapatkan enamdeterminan antigen pada antigen OMP B. abortus isolat lokal.
Penentuan Protein Antigen Limfosit Sapi yang Berasal dari Sapi Bali dan Sapi Peranakan Ongole (DETERMINATION OF THE PROTEIN OF BOVINE LYMPHOCYTE ANTIGEN, OF BALI CATTLE AND ONGOLE CROSS BREED CATTLE) Suwiti, Ni Ketut; Rantam, Fedik Abdul
Jurnal Veteriner Vol 4, No 2 (2003)
Publisher : Jurnal Veteriner

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Efek ekstrak buah delima (Punica Granatum L) terhadap ekspresi wild p53 pada sel ganas rongga mulut mencit strain swiss webster Hernawati, Sri; Rantam, Fedik Abdul; Sudiana, I Ketut; Rahayu, Retno Pudji
Dental Journal (Majalah Kedokteran Gigi) Vol 46, No 3 (2013): (September 2013)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v46.i3.p148-151

Abstract

Background: Squamous cell carcinoma is the most common cancer in the oral cavity. DNA tests showed that almost 90% of cases revealed wild p53 gene mutations. Wild p53 gene mutations cause p53 inactivation so the cell cycle does not stop in G1 phase but continues to S phase and G2 and M, it makes the mutated DNA remains multiplied and apoptosis does not occur. One candidate of the cancer treatment alternatives is pomegranate extract (Punica granatum L – PGL). Purpose: The purpose of study was to examine the effect of PGL on wild p53 expression in oral cavity malignant cell of swiss webster strain mice. Methods: Thirty- two swiss webster strain mice (Balb/c) 5 months old were randomly divided into four groups. Two control groups (K0: no benzopirene exposed and untreated; K1: benzopirene exposed and untreated); and 2 treatment groups (P1: benzopirene exposed and given EA; P2: benzopirene exposed and given PGL extract). The expression of wild p53 was determined by immunohistochemical techniques. Results: The results showed that administration of PGL could increase the expression of wild p53 in malignant epithelial cells in the oral mucosa of mice, and the expression was higher than EA. Conclusion: This study suggested that the PGL extract could express wild p53 in the oral cavity malignant cells of swiss Webster strains mice.Latar belakang: Karsinoma sel skuamosa merupakan kanker yang sering terjadi pada rongga mulut. Pemeriksaan DNA menunjukkan hampir 90% kasus dijumpai adanya mutasi gen wild p53. Mutasi gen wild p53 menyebabkan inaktivasi wild p53 sehingga siklus sel tidak berhenti pada fase G1 tetapi berlanjut ke fase S dan G2 dan M, sehingga DNA yang mengalami mutasi tetap dilipatgandakan dan apoptosis tidak terjadi. Salah satu kandidat obat kanker adalah ekstrak buah delima (Punica Granatum L - PGL). Tujuan: Penelitian ini bertujuan untuk meneliti efek ekstrak PGL terhadap ekspresi wild p53 pada sel ganas rongga mulut mencit strain swiss webster. Metode: Tiga puluh dua ekor mencit (Balb/c) strain swiss webster jantan berumur 5 bulan dibagi secara random menjadi 4 kelompok, yaitu 2 kelompok kontrol (K0: tidak dipapar benzopirene dan tidak diberi perlakuan; K1: dipapar benzopirene dan tidak diberi perlakuan); serta 2 kelompok perlakuan (P1: dipapar benzopirene dan diberi EA; P2: dipapar benzopirene dan diberi ekstrak PGL). Pemeriksaan ekspresi wild p53 dilakukan dengan teknik  imunohistokimia. Hasil: Hasil penelitian menunjukkan bahwa pemberian ekstrak PGL dapat meningkatkan ekspresi wild p53 pada sel epitel ganas pada mukosa rongga mulut mencit, dan lebih tinggi dibanding dengan pemberian EA. Simpulan: Penelitian ini menunjukkan bahwa ekstrak PGL dapat meningkatkan ekspresi wild p53 pada sel ganas rongga mulut mencit strain swiss webster
CHARACTERIzATION of CD4 + T LYMPHOCYTE FROM BONE MAROW STEM CELL USING INDIRECT IMMUNOFLUORESENCE FOR HIV & AIDS TREATMENT Purwati, Purwati; Nasronudin, Nasronudin; Rantam, Fedik Abdul
Indonesian Journal of Tropical and Infectious Disease Vol 1, No 3 (2010)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v1i3.2195

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Acquired immune deficiency syndrome (AIDS) is caused by Human Immunodeficiency Virus (HIV). At the beginning of infection, gp120 virus interacts with CD4 receptor at the surface of the target cell. The interaction between gp120 and CD4 leads to the occurrence of the binding of specific chemokine receptor CXCR4 and CCR5, which are also present on the membrane of the target cell. Therefore, CCR5 and CXCR4 also determine the fate of the target cell. It is the performance of CCR5 and CXCR4, guided by controlling gene that determines susceptibility or resistance to HIV infection. Coding gene CCR5 may mutate to become protective or resistant against HIV infection. In homozygote individuals, it tends to be resistant against infection, while in heterozygote individuals it tends to be susceptible to HIV infection. Objective: To characterize TCD4 lymphocyte in the next that is resistant against HIV infection by using gene therapy deletion 32 CCR5 to use for HIV & AIDS treatment. Method: Sample collection, mononucleated cell collection, lymphocyte culture, CD4 identification, CCR5 variance analysis, co-cultivation with PBMC HIV and comparison to control. Result: This study was performed in several steps, such as mononucleated cell isolation, followed with cell culture, lymphocyte purification, lymphocyte and CD4 expression identification. Conclusion: Lymphocyte T CD4 had been mature after seven passages, once passage is about 5 days so for maturity lymphocyte T CD4 need 35 days and that cell as be candidate to resistant against HIV infection by using gene therapy deletion 32 CCR5 to use for HIV & AIDS treatment.
Utilization of viable bone marrow derivat stem cells through an adaption in low oxigen tension as an attempt to increase cellular transplantation efficacy for spermatogenesis process Safitri, Erma; Utama, Suzanita; Bumi, Candra; Mardi, Sri Wigati; Mulyani, .; Helen, .; Purwati, .; Prasetyo, R. Heru; Hariadi, Mas’ud; Rantam, Fedik Abdul
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Cellular transplantation using stem cells provides very promising solutions in the regeneration and repairment of cells that have experienced degeneration where recovery through medical or surgical intervension is impossible. However, the very low viability of transplanted stem cells limits the transplantation efficacy. The aim of this research was to obtain viable bone marrow derived stem cells by an adaptation treatment in a low oxygen tensioned in vitro culture. Low oxygen tension adaptation was adjusted to the niche of the stem cells in vivo. In this study, in vitro culture of stem cells in 1% oxygen was compared to those of the conventional culture in 21 % oxygen.Results showed that under 1% oxygentension cell proliferation was slower with larger or rounded triangle shaped cells, and senescence or dead cells was low. Meanwhile under 21 % oxygen tension cell proliferation was two fold faster with flattened and slender cells, and senescence or dead cells was higher. In conclusion, conventional in vitro culture under 21% oxygen caused cell aging (senescence) and rapid cell death, therefore the transplanted cells were not viable.
Antibody of goat zona pellucida-3 (gzp3) protein of mice(Mus musculus) block in vitro fertilization of mice as an animal model= Antibodi protein zona pelusida-3 kambing (gZP3) asal mencit(Mus musculus) mencegah ... Mustofa, Imam; Mahaputra, Laba; Dachlan, Yoes Priyatna; Rantam, Fedik Abdul; ., Suwarno; ., Widjiati; Hinting, Aucky
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.22146/jsv.344

Abstract

The researchs of immunocontraception have done in ZP3 of several species, but have not been done in ZP3 of goat. In preliminary study, gZP3 protein was effective prohibited of graviditation of mice. The aim of this study was to prove the potency of gZP3 protein to prohibit in vitro fertilization of mice as an animal model. Antibody of gZP3 produced on mice. Immunized mice serum was analyzed using Elisa and Dot blotting method. Antibody of gZP3 supplemented into M-16 media for oocyte incubation, continued with in vitro fertilization. The result showed that antibody titer of immunized mice serum was higher (p
Ekspresi protein P53 dan HSP70 pada sel punca karsinoma nasofaring yang resisten terhadap radioterapi Yusuf, Muhtarum; Romdhoni, Ahmad Chusnu; Kentjono, Widodo Ario; Rantam, Fedik Abdul
Oto Rhino Laryngologica Indonesiana Vol 44, No 2 (2014): Volume 44, No. 2 July - December 2014
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.659 KB) | DOI: 10.32637/orli.v44i2.93

Abstract

Latar belakang: Pada penderita Karsinoma Nasofaring (KNF) masih sering ditemukan kekambuhan meskipun sudah mendapat terapi yang lengkap. Penelitian terbaru membuktikan bahwa kekambuhan disebabkan oleh sel punca KNF yang resisten terhadap radioterapi. Mekanisme resistensi sel punca kanker terhadap radioterapi diduga karena hambatan terhadap apoptosis dan atau memicu proliferasi. Hambatan terhadap apoptosis disebabkan oleh penurunan protein p53 (wild type), selain over-ekspresiHsp70. Tujuan: Menjelaskan mekanisme resistensi sel punca KNF terhadap radioterapi berdasarkan profil ekspresi protein p53(wild type)dan Hsp70. Metode: Penelitian true experimental dengan menggunakan rancangan randomisasi kelompok kontrol sebelum dan sesudah tes. Kultur sel punca KNF dibagi menjadi dua kelompok, masing-masing 16 sampel. Pada kelompok perlakuan diberikan paparan radioterapi dengan dosis 1,5 Gy menggunakan pesawat Linac, lalu diinkubasi selama 24 jam. Sebelum dan sesudah perlakuan pada kedua kelompok diperiksa ekspresi p53 (wild type) dan Hsp70. Pemeriksaan menggunakan metode flowcytometry. Hasil: Ekspresi p53 (wild type) antara kelompok perlakuan dan kontrol terdapatperbedaan yang tidak bermakna dengan p=0,576 (p≥0,05). Ekspresi Hsp70, antara kelompok perlakuan dan kontrol terdapat perbedaan yang tidak bermakna dengan p=0,172 (p≥0,05). Kesimpulan: Tidak terdapatperubahan ekspresi p53 (wild type) dan Hsp70 pada sel punca KNF yang resisten terhadap radioterapi.Kata kunci : Sel punca KNF, p53 (wild type), Hsp70, karsinoma nasofaring ABSTRACTBackground: Recurrences are frequently occurred in nasopharyngeal  carcinoma (NPC) patients, eventhough they had received complete therapy. Recent studies have proved that those recurrences were caused by NPC cancer stem cells that resistant to radiotherapy. Mechanisms of resistance of cancer stem cells to radiotherapy is assumed due to the block of apoptosis and or proliferation inducing. The block of apoptosis was caused by the decrease of p53 (wild type) expression, in addition to Hsp70 over expression. Objective: To find out the mechanism of NPC stem cells that resistant to radiotherapy based on profiles of protein p53 (wild type) and Hsp70 expression. Methods: Using true experimental study by randomizedpre and post test control group design. The cultured NPC stem cells were divided into two groups, with 16 samples each. The treatment group had 1,5Gy dose of radiotherapy exposure with Linac device, then incubated for 24 hours. Both groups were examined for p53 (wild type) and Hsp70 expressions before and after treatment. The examinations were conducted by flowcytometry method. Result: The P53 (wild type) expression between the treatment and control group showed insignificant difference with p=0.576(p≥0.05). The Hsp70 expression between treatment and control group showed insignificant difference with p=0.172 (p≥0.05). Conclusion: There were no changes of p53 (wild type) and Hsp70 expressions on NPC stem cells that resistant to radiotherapyKeywords: NPC stem cells, p53 (wild type), Hsp70, nasopharyngeal carcinoma