Articles

Found 18 Documents
Search

Automated Detection of Binucleated Cell and Micronuclei using CellProfiler 2.0 Software RAMADHANI, DWI; PURNAMI, SOFIATI
HAYATI Journal of Biosciences Vol 20, No 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (573.964 KB)

Abstract

Micronucleus assay in human peripheral lymphocytes usually used to assess chromosomal damage. Manual scoring of micronuclei can be time consuming and large numbers of binucleated cells have to be analyzed to obtain statistically relevant data. Automation of the micronuclei analysis using image processing analysis software can provide a faster and more reliable analysis of micronucleus assay. Here the used of CellProfiler an open access cell image analysis software for automatic detection of binucleated cells and micronuclei were reported. We aimed to know whether there was a significant difference in the number of binucleated cells and micronuclei that obtained by manual and CellProfiler counting. Wilcoxon Rank test was used for statistical analysis to test H0 hypothesis that there was no significant difference in the number of binucleated cells and micronuclei that obtained by manual and CellProfiler counting. We analyzed 135 images for both manual and CellProfiler counting. Our results showed that there was no significant difference between manual and CellProfiler counting for binucleated cells (P = 0.851) and for micronuclei (P = 0.917). In conclusion, the binucleated cells and micronuclei counting using CellProfiler were comparable but not better than manual counting.
ABERASI KROMOSOM PADA PILOT DAN AWAK KABIN MASKAP AI PENERBANGAN KOMERSIAL Ramadhani, Dwi; Purnami, Sofiati; Suvifan, Viria Agesti
Buletin Alara Vol 13, No 2: Desember 2011
Publisher : BATAN

Show Abstract | Original Source | Check in Google Scholar | Full PDF (2351.169 KB)

Abstract

Tidak ada abstrak
RESPON SITOGENETIK PENDUDUK DAERAH RADIASI ALAM TINGGI DI KABUPATEN MAMUJU, SULAWESI BARAT A., Zubaidah; Lusiyanti, Yanti; P., Sofiati; Ramadhani, Dwi; L., Masnelly; AS., Viria
Jurnal Sains dan Teknologi Nuklir Indonesia Vol 13, No 1 (2012): Februari 2012
Publisher : BATAN

Show Abstract | Original Source | Check in Google Scholar

Abstract

RESPON SITOGENETIK PENDUDUK DAERAH RADIASI ALAM TINGGI DI KABUPATEN MAMUJU, SULAWESI BARAT. Manusia di dunia menerima paparan radiasi alam baik eksternal maupun internal. Total paparan tahunan dari radiasi alam dengan latar normal adalah 2,4 mSv, sedangkan daerah dengan tingkat paparan radiasi alam mencapai 20 mSv atau lebih dikategorikan sebagai High Natural Background Radiation (HNBR). Tingkat paparan radiasi di HNBR dianggap sebagai radiasi dosis rendah. Efek biologik radiasi dosis rendah khususnya efek sitogenetik telah dipelajari pada penduduk di beberapa daerah HNBR di dunia. Tulisan ini melaporkan hasil studi sitogenetik khususnya aberasi kromosom pada sel limfosit darah tepi penduduk di Kabupaten Mamuju, Sulawesi Barat, yang mempunyai tingkat paparan radiasi alam relatif tinggi di Indonesia. Pemeriksaan aberasi kromosom dilakukan dengan teknik Giemsa dan Fluoresence in situ Hybridization terhadap sampel darah penduduk yang telah dibiakkan dalam media pertumbuhan. Hasil penelitian tidak menunjukkan adanya perbedaan nyata frekuensi aberasi kromosom pada sel limfosit penduduk daerah radiasi alam tinggi dan latar normal. Terdapat indikasi adanya fenomena respon radioadaptif pada penduduk di Kabupaten Mamuju yang perlu diteliti lebih lanjut. Kata kunci : dosis rendah, aberasi kromosom, HNBR, Mamuju. ABSTRACT CYTOGENETIC  RESPONSE OF THE RESIDENTS OF HIGH NATURAL RADIATION AREA IN KABUPATEN MAMUJU, SULAWESI BARAT. People in the world are exposed to background  radiation from natural sources both internally and externally. Total exposure to natural radiation in areas of normal background averages 2.4 mSv per year, while the area that has total exposure to natural radiation up to 20 mSv or more is categorized as the High Natural Background Radiation (HNBR). Such radiation level of HNBR is considered as low dose radiation.The biological effects of low dose radiation, especially cytogenetic effects, have been extensively studied on the habitants of several HNBR areas in the world. This paper reports the results of cytogenetic study, especially chromosome aberrations, in peripheral blood lymphocyte cells of  the residents in Kabupaten Mamuju, West Sulawesi, which relatively has high level of natural radiation in Indonesia. Chromosome aberration examinations were performed using Geimsa staining method and fluorescence in situ hybridization painting technique after culturing the blood samples of the residents in enriched growth media. The results did not indicate significant difference on the frequency of chromosome aberrations in the lymphocytes of  people who live in HNBR area and that who live in normal natural radiation area. It is suggested that there is an indication of radioadaptive response phenomena in Kabupaten Mamuju people that needs further investigation. Keywords: low dose, chromosome aberration, HNBR, Mamuju
Haemozoin Detection in Mouse Liver Histology Using Simple Polarized Light Microscope RAMADHANI, DWI; NURHAYATI, SITI; RAHARDJO, TUR
HAYATI Journal of Biosciences Vol 21, No 1 (2014): March 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (877.9 KB)

Abstract

The presence of malarial pigment (haemozoin) due to Plasmodium infection is a common histopathological effect in mouse liver. Previous research showed that by using a polarized light microscope, researchers were better able to detect haemozoin in mouse liver histology section. Thus, the aim of this research was to compare the haemozoin area observed by a conventional vs. simple polarized light microscope by using image processing analysis. A total of 40 images produced from both conventional light microscope and simple polarized light microscope were collected. All images were analyzed using ImageJ 1.47 software to measure the haemozoin areas. Our results showed that non birefringent haemozoin and birefringent haemozoin area was significantly different. This was because when using conventional light microscope the brown area that contained images of non birefringent haemozoin images also contained Kupffer cells which appeared as the same brown color as haemozoin. In contrast, haemozoin gave bright effect and can be easily differentiated with Kupffer cells in the birefringent haemozoin images. This study concluded that haemozoin detection in mouse liver histology using a simple polarized light microscope was more accurate compared to that of conventional light microscope.
Liver Histophatological Studies of Mice (Mus musculus sp.) Infected With Gamma Rays Irradiated Plasmodium berghei Strains Anka RAHARDJO, TUR; NURHAYATI, SITI; RAMADHANI, DWI
HAYATI Journal of Biosciences Vol 20, No 3 (2013): September 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (156.115 KB)

Abstract

Malaria is a serious global disease and a leading cause of morbidity and mortality in tropical and sub-tropical countries. Radiation attenuated vaccines have been demonstrated to be an effective means of controlling certain parasitic infections such as malaria. Our previous research showed that irradiation dose of 150-175 Gy to Plasmodium berghei were enable to suppress the infected erythrocyte cells and irradiation dose at 150 Gy was more effective compared to 175 Gy in attenuate the parasites based on parasitemia index. Aim of this research was to investigate the most suitable irradiation dose to attenuated P. berghei based on the the histological changes in the liver mouse malaria model. Histological changes in mice liver because of Plasmodium is congestion in the sinusoids and masses of hemozoin. Fourty Swiss mice were randomly assigned to four equal groups. First group of mice were inoculated with the unirradiated malaria parasites as a positive control while negative control group represented uninfected normal control. The third group of mice was inoculated with the irradiated malaria parasites with dose 175 Gy. Last group were inoculated with the irradiated malaria parasites with dose 150 Gy. Mice were sacrificed at day 6, 8, 12, 16, 20, and 24 post inoculation for liver histological study. Their livers processed for histomorphometric assessment of sinusoidal area, haemozoin area and hepatosomatic index as a quantitative measure of altered morphology. Hepatic sinusoidal area in mice infected with the unirradiated malaria parasites increased by 104% in day 24 post inoculated compared to day 6 post inoculated, whereas a smaller increase of 46% occurred in mice that were inoculated with the irradiated malaria parasites with dose 150 Gy. The difference between positive control and 150 Gy group was significant (P < 0.05).  The highest average haemozoin area in all days post inoculation was in positive control whereas a smaller occurred in mice that were inoculated with the irradiated malaria parasites with dose 175 Gy, but the different was not significant (P > 0.05). For hepatosomatic index (HSI) at day 24 post inoculation the HSI value in group 150 Gy (5.33) was the smallest compared to other groups. Based on our research it can be concluded that irradiation dose at 150 Gy was more effective compared to 175 Gy in attenuate the parasites based on assessment of sinusoidal area, and HSI value at 24 day post inoculation.
PEMAHAMAN KONSEP MATEMATIS SISWA PADA MATERI KESEBANGUNAN MELALUI PEMBELAJARAN COOPERATIVE TIPE NUMBERED HEADS DISERTAI LKS Ramadhani, Dwi; Haryono, Yulia; Lovia, Lita
Pendidikan Matematika Vol 2, No 2 (2013): Jurnal Wisuda Ke 47, Genap 2013-2014 Pendidikan Matematika
Publisher : STKIP PGRI Sumbar

Show Abstract | Original Source | Check in Google Scholar

Abstract

The research is introduced by the lack of the students understanding of mathematical concept and students prefer to copy paste their friends exercise without paying attention it is right or wrong. This research is aimed to know the students understanding of mathematicalconcept by using learning model of Cooperative type Numbered Heads with LKS better than the students understanding concept by using conventional learning at class IX SMP PERTI Padang. The type of this research is experiment. Population in this research is students class IX SMP PERTI Padang who registered in academic year 2013/2014 that consists of three class. The tecgnique of sampling is random and selected class IX.1 as an experimental class and class IX.2 as control class. Insrument used was final test contain mathematical concept umderstanding indicators in the from of essay. Technique of data analysis used was Mann-Withney test with MINITAB sofware. Based on the hypothesis obtained P-value = 0,003, because p-less than α = 0,05 so the hypotesisis accepted the students understanding of mathematical concept by using Cooperative type Numbered Heads learning with LKS is better than students understanding mathematical concept by using conventional learning at class IX SMP PERTI Padang.
SEMI OTOMATISASI KARIOTIPE UNTUK DETEKSI ABERASI KROMOSOM AKIBAT PAPARAN RADIASI Ramadhani, Dwi; Lusiyanti, Yanti; Alatas, Zubaidah; Purnami, Sofiati
Jurnal Forum Nuklir JFN Vol 6 No 2 November 2012
Publisher : BATAN

Show Abstract | Original Source | Check in Google Scholar

Abstract

SEMI OTOMATISASI KARIOTIPE UNTUK DETEKSI ABERASI KROMOSOM AKIBAT PAPARAN RADIASI. Proses analisis citra kromosom dilakukan dengan mengklasifikasikan kromosom berdasarkan panjang dan bentuknya sehingga dihasilkan ideogram. Proses tersebut dinamakan kariotipe. Kariotipe umumnya dilakukan dengan cara mengambil citra sel pada saat metafase sehingga kromosom terlihat jelas terlebih dahulu, kemudian menggunting setiap citra kromosom dan mengidentifikasi masing­masing kromosom untuk dibuat ideogramnya. Proses kariotipe dapat digunakan untuk mendeteksi aberasi kromosom akibat paparan radiasi. Proses pembuatan kariotipe sangat menyita waktu dan tenaga sehingga telah banyak dikembangkan perangkat lunak untuk membantu kariotipe kromosom baik yang otomatis maupun semiotomatis. Salah satu perangkat lunak tersebut adalah Cytovision 3.6. Selain perangkat lunak yang bersifat komersil terdapat perangkat lunak lain yang dapat di unduh dan digunakan secara bebas uotuk membantu proses kariotipe yaitu SmartType Express. Tujuan kegiatan yang dilakukan adalah untuk membandingkan kemampuan perangkat lunak semi otomatis komersil dan yang bersifat bebas dalam membantu proses pembuatan ideogram untuk mendetek si aberasi kromosom akibat paparan radiasi. Metode yang digunakan adalah membandingkan waktu yang dibutuhkan oleh Cytovision 3.6 dan SmartType Express untuk menghilangkan kesepuluh background citra dan meningkatkan intensitas warna gelap pada pita kromosom juga pemisahan citra kromosom tumpang tindih hingga terbentuk citra kromosom yang lebih mudah untuk diklasifikasi dan dibuat ideogramnya. Data yang didapat kemudian diolah secara statistik menggunakan Uji Wilcoxon dengan hipotesis bahwa H0 adalah tidak terdapat perbedaan waktu secara nyata menggunakan kedua perangkat lunak untuk menghasilkan citra kromosom yang lebih baik dan H1 terdapat perbedaan secara nyata menggunakan kedua perangkat lunak untuk menghasilkan citra kromosom yang lebih baik. Taraf nyata yang digunakan (a) adalah 0,05. Hasil pengolahan secara statistik menunjukkan bahwa pengolahan citra kromosom mengunakan SmartType Express cukup baik dan tidak berbeda dengan perangkat lunak komersil Cytovision 3.6.
Analisa Efektifitas Fasilitas Zebra Cross Pada Jl. MT Haryono dan Jl. Gajayana Arifin, M. Zainul; Prawito, Gagoek Soenar; Ramadhani, Dwi
Rekayasa Sipil Vol 1, No 1 (2007)
Publisher : Department of Civil Engineering, Faculty of Engineering, University of Brawijaya

Show Abstract | Original Source | Check in Google Scholar | Full PDF (115.062 KB)

Abstract

Kemajuan Kota Malang yang semakin pesat kurang diimbangi dengan fasilitas jalan yang memadai terutama untuk pejalan kaki. Berdasarkan data kepolisian, rata-rata kecelakaan di JL. MT. Haryono dan Jl. Gajayana Malang melibatkan pengendara kendaraan bermotor dengan penyeberang jalan. Penyeberang jalan kurang memanfaatkan fasilitas zebra cross untuk menyeberang. Begitu pula dengan pengendara kendaraan bermotor, mereka cenderung kurang mengutamakan penyeberang jalan ketika akan menyeberang, kecepatan mereka relatif konstan. Maka dari itu diadakan penelitian untuk mengetahui efektifitas dari zebra cross apakah keberadaannnya sudah benar-benar tepat, sehingga dapat diambil sebagai dasar pengambilan tindakan dalam mengatasi masalah kecelakaan lalu lintas. Tujuan penelitian ini adalah untuk mengetahui efektifitas fasilitas zebra cross dengan parameter penurunan kecepatan kendaraan pada saat akan melewati zebra cross.Dari hasil perhitungan statistik, pada periode waktu pertama didapatkan kecepatan kendaraan pada jarak 30 m adalah 38,63150 km/jam dan pada jarak 50 m adalah 37,00380 km/jam. Sedangkan kecepatan kendaraan pada periode waktu kedua pada jarak 30 m adalah 37,3452 km/jam dan pada jarak 50 m adalah 35,5920 km/jam. Hal ini menunjukkan bahwa pada periode waktu pertama dan kedua, kecepatan kendaraan pada jarak 30 m lebih besar dibandingkan dengan kecepatan pada jarak 50 m. Jadi kesimpulannya zebra cross di Jl. MT Haryono dan Jl. Gajayana tidak efektif, berdasarkan tidak adanya penurunan kecepatan kendaraan pada saat akan melewati zebra cross. Kecepatan kendaraan cenderung naik pada saat akan melewati zebra cross. 
Haemozoin Detection in Mouse Liver Histology Using Simple Polarized Light Microscope RAMADHANI, DWI; NURHAYATI, SITI; RAHARDJO, TUR
HAYATI Journal of Biosciences Vol 21, No 1 (2014): March 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (877.9 KB)

Abstract

The presence of malarial pigment (haemozoin) due to Plasmodium infection is a common histopathological effect in mouse liver. Previous research showed that by using a polarized light microscope, researchers were better able to detect haemozoin in mouse liver histology section. Thus, the aim of this research was to compare the haemozoin area observed by a conventional vs. simple polarized light microscope by using image processing analysis. A total of 40 images produced from both conventional light microscope and simple polarized light microscope were collected. All images were analyzed using ImageJ 1.47 software to measure the haemozoin areas. Our results showed that non birefringent haemozoin and birefringent haemozoin area was significantly different. This was because when using conventional light microscope the brown area that contained images of non birefringent haemozoin images also contained Kupffer cells which appeared as the same brown color as haemozoin. In contrast, haemozoin gave bright effect and can be easily differentiated with Kupffer cells in the birefringent haemozoin images. This study concluded that haemozoin detection in mouse liver histology using a simple polarized light microscope was more accurate compared to that of conventional light microscope.
Automated Detection of Binucleated Cell and Micronuclei using CellProfiler 2.0 Software RAMADHANI, DWI; PURNAMI, SOFIATI
HAYATI Journal of Biosciences Vol 20, No 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (573.964 KB)

Abstract

Micronucleus assay in human peripheral lymphocytes usually used to assess chromosomal damage. Manual scoring of micronuclei can be time consuming and large numbers of binucleated cells have to be analyzed to obtain statistically relevant data. Automation of the micronuclei analysis using image processing analysis software can provide a faster and more reliable analysis of micronucleus assay. Here the used of CellProfiler an open access cell image analysis software for automatic detection of binucleated cells and micronuclei were reported. We aimed to know whether there was a significant difference in the number of binucleated cells and micronuclei that obtained by manual and CellProfiler counting. Wilcoxon Rank test was used for statistical analysis to test H0 hypothesis that there was no significant difference in the number of binucleated cells and micronuclei that obtained by manual and CellProfiler counting. We analyzed 135 images for both manual and CellProfiler counting. Our results showed that there was no significant difference between manual and CellProfiler counting for binucleated cells (P = 0.851) and for micronuclei (P = 0.917). In conclusion, the binucleated cells and micronuclei counting using CellProfiler were comparable but not better than manual counting.