K. B. Ramachandran
Department of Chemical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia

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Effect of Inoculum Age, Carbon and Nitrogen Sources on the Production of Lipase by Candida Cylindracea 2031 in Batch Fermentation Noor, I. M.; Hasan, M.; Ramachandran, K. B.
Jurnal Rekayasa Kimia & Lingkungan Vol 5, No 1 (2006): Jurnal Rekayasa Kimia & Lingkungan
Publisher : Chemical Engineering Department, Syiah Kuala University, Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.219 KB)

Abstract

Production of extracellular lipase by Candida cylindracea DSMZ 2031  was studied in a seven liters batch bioreactor, using palm oil (PO), palmitic acid (PA), lauric acid (LA), olive oil (OO) and cooking oil (CO) as carbon source.   The effect of  carbon and nitrogen sources  were studied by measuring the lipase activity.  The maximum lipase activity was found to be 12.7 kLU on palm oil as carbon source, urea as nitrogen sources and at 36 h inoculum age. This was achieved at a temperature of 30o C, pH of 6.0, agitation speed of 500 rpm and aeration of 1vvm.
Production of Medium Chain Length Polyhydroxyalkanoates From Oleic Acid Using Pseudomonas putida PGA1 by Fed Batch Culture Marsudi, Sidik; Tan, Irene K. P.; Gan, Seng-Neon; Ramachandran, K. B.
Makara Journal of Technology Vol 11, No 1 (2007)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7454/mst.v11i1.119

Abstract

Bacterial polyhydroxyalkanoates (PHAs) are a class of p0lymers currently receiving much attention because of their potential as renewable and biodegradable plastics. A wide variety of bacteria has been reported to produce PHAs including Pseudomonas strains. These strains are known as versatile medium chain length PHAs (PHAs-mcl) producers using fatty acids as carbon source. Oleic acid was used to produce PHAs-mcl using Pseudomonas putida PGA 1 by continuous feeding of both nitrogen and carbon source, in a fed batch culture. During cell growth, PHAs also accumulated, indicating that PHA production in this organism is growth associated. Residual cell increased until the nitrogen source was depleted. At the end of fermentation, final cell concentration, PHA content, and roductivity were 30.2 g/L, 44.8 % of cell dry weight, and 0.188 g/l/h, respectively.