T.N. Ralahalu
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PENGUJIAN UNDUR-UNDUR LAUT (Emerita analoga) SEBAGAI BAHAN PENURUN KOLESTEROL PADA MENCIT (Mus musculus BALB/C) Kardaya, D.; Ralahalu, T.N.; Zubir, -; Purba, M.; Parakkasi, A.
JURNAL ILMU DAN TEKNOLOGI PETERNAKAN Vol 1, No 2 (2011)
Publisher : Fakultas Peternakan Universitas Hasanuddin

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Abstract

A reasonable effort to reduce cholesterol content of meat could be done by ration manipulation. Sea animals are known having high free fatty acid of omega 3 and 6 series. These nutrients could reduce meat cholesterol level if included in ration. The study aimed to reveal the effect of various level of sand crab (Emerita analoga) powder supplemented in ration on performances and meat cholesterol level of Mus musculus balb/c mouse. Thirty two mice, consisted of 16 male and 16 female of a 28-day old, were fed on one of four different rations with four replications in a factorial arrangement according to completely randomized design. The rations contained isoprotein and isoenergy but with different level of sand crab powder, i.e. 0, 12.5, 25, and 37.5% of the ration dry matter. The mice were treated for five weeks and each week body weight or orts was measured.  At the end of experimental period, the mice were slaughtered and carcassed and each of the right leg was sampled for meat cholesterol analysis. The data collected were analyzed with analyzes of variance and Duncan’s multiple range test was applied to separate the different means of each treatment. In addition, a polynomial orthogonal regression was applied to predict optimal level of sand crab supplementation. Result of the study revealed that experimental ration affected (P<0.05) feed consumption, weight gain, feed conversion, and meat cholesterol level. Moreover, feed consumption, weight gain, and feed conversion were affected (P<0.01) by sex of the mouse. Meanwhile, meat cholesterol level was not affected by sex. The experimental rations containing 25% of sand crab powder resulted in the best performances of the mouse and the optimal level predicted from polynomial graph was 23.02%.   Key words: Sand crab, Emerita analoga, Cholesterol, Mouse
PENGARUH PEMBERIAN AMPAS SAGU, LIMBAH UDANG DAN KOMBINASINYA TERHADAP KADAR KOLESTEROL DAN PERTUMBUHAN TIKUS PUTIH Ralahalu, T.N.; Kartiarso, -; Parakkasi, A.; Wiryawan, K.G.; Priyanto, R.
JURNAL ILMU DAN TEKNOLOGI PETERNAKAN Vol 1, No 3 (2011)
Publisher : Fakultas Peternakan Universitas Hasanuddin

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Cholesterol is very beneficial for the body but it needs attention when its level is increasing in the blood. The objective of the study was to determine cholesterol level and growth rate of white rat fed on ration containing sago waste, shrimp waste or their combinations. Fourty males Spraque dowley rats, aged two months with average body weight 195.62 g, were randomly assigned to one of ten experimental treatments according to completely randomised design with four replication for each treatment combination. The treatments were  R0 (control diet), ASA 20 LU 0 (20% sago waste  and 0% shrimp waste),  ASA 0 LU 10 (0% sago waste and 10% shrimp waste), ASA 17.5 LU 2.5 (17.5% sago waste and 2.5% shrimp waste), ASA 15 LU 5 % (15% sago waste  and 5% shrimp waste), ASA 12.5 LU 7.5  (12.5% sago waste  and 7.5% shrimp waste),  ASA 10 LU 10 (10% sago waste  and 10% shrimp waste), ASA 7.5 LU 12.5  (7.5% sago waste and 12.5% shrimp waste), ASA 5 LU 15  (5% sago waste and 15% shrimp waste %), ASA 2.5 LU 17.5 (2.5% sago waste  and 17.5% shrimp waste).Variables measured included feed intake, live weight gain, plasma cholesterol, HDL, LDL, triglyceride and meat cholesterol. The results showed that treatments had no (P>0.05) effects on feed intake, live weight gain, feed efficiency, plasma HDL and LDL as well as meat cholesterol. However significant differences (P<0.01) were found on plasma cholesterol and plasma triglyceride due to treatment. The lowest levels of plasma cholesterol and plasma triglyceride were observed for treatment ASA 15 LU which were 45.95 mg/dl and 43.81 mg/dl,crespectively. It can be concluded that levels of sago waste, shrimp waste and the combinations in the ration did not affect feed intake, weight gain, feed efficiency, plasma HDL, plasma LDL and meat cholesterol but it affected plasma triglycerides and plasma cholesterol of rat. Key words: Sago waste, Shrimp waste, Rat