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Changes of Gibberellin and Total Sugar Content in Flower Developmental Stages of Mangosteen RAI, I NYOMAN; POERWANTO, ROEDHY; DARUSMAN, LATIFAH KOSIM; PURWOKO, BAMBANG SAPTA
HAYATI Journal of Biosciences Vol 13, No 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The objectives of this experiment were to study the changes of gibberellic acid and total sugar content in flower developmental stages of mangosteen. The result showed that flower development of mangosteen consisted of four stages: induction, differentiation, maturition of flower organs, and anthesis. Floral induction was microscopically characterized by the swelling of the basal structure of the new shoot. It was found that induction stage of mangosteen flowering was characterized by sharp decrease of gibberellic acid (GA3, GA5, GA7) and increase of total sugar content of leaf. On the other hand, it was found that leaf of the non-flowering shoot apices had high gibbrellic acid and low total sugar. Key words: mangosteen, flowering, induction, gibberellic acid
BUNGA DAN BUAH GUGUR PADA TANAMAN MANGGIS (GARCINA MANGOSTANA L.) ASAL BIJI DAN SAMBUNGAN RAI, I NYOMAN
AGRITROP Vol. 26, No. 2 Juni 2007
Publisher : AGRITROP

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Abstract

Grafted mangosteen tree have a shorter juvenile period than that of the seedling trees because it starts to flower earlier. However, productivity of the grafted trees is low because number of flowers and fruits drop is high. The research was conducted to study the difference of flowers and fruit drop between seedling and grafted trees related to N, P, and K plant nutrition levels and position of growing flower and fruit. The result showed that percentage of flowers and fruits drop of grafted mangosteen (14,65% and 85,38%) were higher than those of seedling plants (6,93% and 16,58%). Yield of grafted tree (2,17 kg/plant) was significanly lower than that of seedling (7,07 kg/plant) exhibited by low number of harvesting fruit. N, P, and K nutrition leaf levels had no effect on flower and fruit number drop, not only on grafted trees, but also on seedling trees. Flowers and fruits which grow at the basal of tree and/or branch drop were hihger than those of midle and upper part of trees and branchs.
Pengaturan Pembungaan Tanaman Manggis (Garcinia mangostana L.) di Luar Musim dengan Strangulasi, serta Aplikasi Paklobutrazol dan Etepon Rai, I Nyoman; Poerwanto, Roedy; Darusman, Latifah K.; Purwoko, Bambang S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 32, No 2 (2004): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

The objective of the research was to study the application of strangulation, packlobutrazol and ethephon to stimulate flowering of mangosteen trees. The experiment was conducted from June 2002 to September2 003, located at Farmer´s Mangosteen Orchards, Leuwiliang, Bogor. A randomized block design with two factors was used in thisexperiment. The first factor was treatments to stimulate flowering consisted of three levels i.e strangulation (8), (S) application of paclobubtrazol (P) and control (K). The second factor was treatments for dormancy breaking consisted of two levels i.e without ethephon (Eo) and with ethephon (EiJ. Each treatment was replicated four times. The resultshowed that strangulation and application of paclobutrazol were effective in stimulating flowering of mangosteen trees. Those treatments decreased gibberellic acid content and increased total sugar and C: N ratio. Interaction effect was found between treatments to stimulate flowering and ethephon on number of flowers, number of fruits and weight of fruits per tree. The highest fruits weight (2.,50 kg/tree) was obtained on the combination of paclobutrazol with ethephon while the lowest was in control without ethephon( 6.05 kg/tree).Key words: Mangosteen, Strangulation, Paclobutrazol, Ethephon, Flowering;
Pengaruh Komposisi Campuran Bahan Media Tanam dan Konsentrasi IBA terhadap Pertumbuhan Bibit Wani Ngumpen Bali (Mangifera caesia Jack) MAHARDIKA, I KADEK DWI; RAI, I NYOMAN; WIRATMAJA, I WAYAN
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.2, April 2013
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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Abstract

ABSTRACT The Influence of Composition Mixed Media Materials Plant and IBA Concentration on Growth of Seedlings Ngumpen Wani Bali (Mangifera caesia Jack) The experiment was conducted at plastic house at the Experimental Farm of the Faculty of Agriculture Udayana University Pegok, from January to May 2012. The objective of research was to determine the best concentration of growing media and plant growth regulators IBA to growth of seedlings wani Ngumpen Bali. The study used a randomized block design (RBD) with two factors. The first factor was growing media compositions comprising three types of media: soil + mixture of sand + organic matter (v: v: v = 3:2:1), soil + mixture of sand + organic matter (v: v: v = 3:1:2) and soil + mixture of sand + organic matter (v: v: v = 2:3:1). The second factor was four level of IBA i.e : 0 ppm, 50 ppm, 100 ppm, and 200 ppm. The results showed that treatment of growing media was not significant effect on plant height, number of leaves, stem segments, flushing cycle, stem diameter, leaf area and leaf NPK content of nutrients. While the concentration of IBA treatment was highly significant for plant height. Interactions between growing media and IBA concentrations was significantly on the nutrient content of N leaves only. The highest plant height was obtained at the 100 ppm IBA concentration (52.13 cm), while the lowest was obtained at control (36.74 cm). Keywords: Growing Media, IBA, Seeds, and Wani Ngumpen Bali
Biodiversity of Bali Banana (Musaceae) and its Usefulness Rai, I Nyoman; Dwivany, Fenny M.; Sutanto, Agus; Meitha, Karlia; Sukewijaya, I Made; Ustriyana, I Nyoman Gede
HAYATI Journal of Biosciences Vol 25, No 2 (2018): April 2018
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Banana (Musa spp.) is one the most important agriculture commodities in Indonesia. Archeological and molecular evidences suggest that speciation of this herb occurred in Indonesia, leading to the high diversity in the archipelago. In Bali Island, banana is not only sought for food but as well as for their symbolic role in religious and cultural ceremonies. However, the high demand for bananas in Bali is not yet supported by the adequate production of local farms. This presented study aimed to investigate the diversity of banana cultivars or sub-species in Bali and its usefulness to determine preferable cultivars to cultivate. We recorded and characterized 43 banana cultivars in 10 villages that represent the 8 regencies and 1 city of Bali province. Out of the 43 cultivars, 7 were highly used and at least one cultivar was discovered in each of the studied village.? The presence of these cultivars in the study areas were confirmed by site visit and characterization of the fruits. Among the highly ranked cultivars or species, only biu kayu is unique to Bali as it was not found in the closest provinces of East Java and Madura. Hence, the results suggested that to improve the cultivation and production of these 7 highly used cultivars could be an appropriate solution to meet Bali demand of bananas. Furthermore, cultivating biu kayu would also help conservation effort since this cultivar is also currently listed as a rare genetic resource.
Identifikasi Variabilitas Genetik Wani Bali (Mangifera caesia Jack.) dengan Analisis Penanda RAPD Rai, I Nyoman; Wijana, G; Semarajaya, C G A
Jurnal Hortikultura Vol 18, No 2 (2008): Juni 2008
Publisher : Indonesian Center for Horticultural Research and Development

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Abstract

ABSTRAK. Wani Bali (Mangifera caesia Jack.) merupakan salah satu tanaman buah-buahan tropika tergolong kerabat mangga. Citarasa Wani Bali disukai konsumen karena daging buahnya memiliki aroma khas, rasanya manis, enak, dan daging buahnya tebal. Terdapat banyak kultivar Wani Bali dengan sifat spesifik buahnya masing-masing, tetapi secara genetik belum diketahui variabilitasnya. Penelitian ini betujuan mengidentifikasi variabilitas genetik Wani Bali dengan analisis penanda random amplified polymorphic DNA (RAPD). Penelitian dilakukan mulai bulan Februari sampai Desember 2006, berlokasi di seluruh sentra produksi Wani Bali di Bali. Pelaksanaan terdiri atas 3 tahap, yaitu (1) survei kultivar dilanjutkan dengan identifikasi karakter daun, bunga, dan buah, (2) pengumpulan sampel untuk analisis RAPD (biji dari kultivar yang telah diidentifikasi ditanam dalam polibag di rumah plastik, setelah bibit berumur 6 bulan, 5-6 lembar daunnya dipanen untuk sampel), dan (3) analisis penanda RAPD, dilakukan di Laboratorium Biomolekuler dan Immunologi, Unit Penelitian Bioteknologi Perkebunan, Bogor. Berdasarkan karakter buahnya (bentuk, rasa, ukuran, dan warna kulit) teridentifikasi 22 kultivar Wani Bali, tetapi kultivar-kultivar tersebut tidak dapat dibedakan satu dengan yang lainnya berdasarkan habitus pohon, sifat percabangan, serta karakter daun dan bunga. Variabilitas genetik Wani Bali dianalisis dengan RAPD dengan keanekaragaman mencapai 43% terdiri atas 3 kelompok. Satu-satunya kultivar yang secara genetik sangat berbeda dengan yang lainnya adalah Wani Bali Ngumpen (kultivar tanpa biji) ditemukan di Desa Bebetin, Kabupaten Buleleng. Kultivar-kultivar yang berasal dari kabupaten yang sama dan atau pada 2 kabupaten yang berdekatan mengelompok pada kelompok yang sama, kecuali Wani Bali Ngumpen asal Desa Bebetin, Buleleng.ABSTRACT. Rai, I.N., G. Wijana, and C. G. A. Semarajaya. 2008. Identification of Genetic Variability of Wani Bali (Mangifera caesia Jack.) Using RAPD Analysis Marker. Wani Bali is one of tropical fruit which belongs to genus mangifera. Consumer prefers the fruit due to the specific flavor, sweet and delicious taste, and the thickness of edible pulp. There are many cultivars of Wani Bali with specific character. However, genetic variability has not been specified. The research was aimed to identify the genetic variability by random amplified polymorphic DNA (RAPD) marker. The research was conducted from February to December 2006, located at centrals of Wani Bali in Bali. It consisted of 3 steps (1) surveying of cultivars and identification of their leaf, flower, and fruit characters, (2) collecting sample for RAPD analysis (seed of identified cultivars grown in polybag at plastichouse and after 6 months seedling, 5-6 leaves were collected as sample), and (3) analyzing RAPD, which was conducted at Biomolecular and Immunology Laboratory, Research Unit of Plantation Biotechnology, Bogor. The results revealed that according to the fruit character (shape, taste, size, and skin color of fruit) had been identified 22 cultivars, but among cultivars could not be specified by plant shape, branch type, leaf and flower characters. There are 3 groups at 43% variability according to genetic variability of Wani Bali which was analyzed by RAPD. The sole cultivar genetically significantly different among the cultivars is Wani Bali Ngumpen (seedles cultivar) from Bebetin, Buleleng District. The cultivars that were planted at the same regency and/or at 2 neighbouring regencies genetically were clustered in 1 group, excluding Wani Bali Ngumpen from Bebetin, Buleleng District.
Changes of Gibberellin and Total Sugar Content in Flower Developmental Stages of Mangosteen RAI, I NYOMAN; POERWANTO, ROEDHY; DARUSMAN, LATIFAH KOSIM; PURWOKO, BAMBANG SAPTA
HAYATI Journal of Biosciences Vol 13, No 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The objectives of this experiment were to study the changes of gibberellic acid and total sugar content in flower developmental stages of mangosteen. The result showed that flower development of mangosteen consisted of four stages: induction, differentiation, maturition of flower organs, and anthesis. Floral induction was microscopically characterized by the swelling of the basal structure of the new shoot. It was found that induction stage of mangosteen flowering was characterized by sharp decrease of gibberellic acid (GA3, GA5, GA7) and increase of total sugar content of leaf. On the other hand, it was found that leaf of the non-flowering shoot apices had high gibbrellic acid and low total sugar. Key words: mangosteen, flowering, induction, gibberellic acid
PHENOTYPIC, GENOTYPIC CHARACTERS AND NUTRITIONAL VALUE OF SEEDLESS WANI (Mangivera caesia Jack. var. Ngumpen Bali) (A Review) Rai, I Nyoman; Semarajaya, Cokorda Gede Alit; Wijana, Gede; Wiraatmaja, I Wayan; Astawa, Ngurah Gede; Astiari, Ni Komang Alit
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

The diversity of Mangivera caesia Jack (Balinese name: wani) in Bali was quite high. Based on the morphological characters of the fruit, 22 cultivars had been explored in the previous research (Rai et al., 2008). One of the most superior cultivar among those and very potential to be commercially developed was seedless wani (M. caesia Jack. var. Ngumpen Bali). The cultivar had specific properties that were not possessed by the others. Ninety (90) % of the total fruits produced were seedless, while the remaining (10%) has small seed. Beside that, this seedless cultivar had thick flesh, very attractive skin color (glossy yellowish green), uniformity on the size and shape of fruits, a distinctive aroma, sweet, tasty, and highly nutritious. The results of RAPD analysis of 10 wani cultivars grown in Bali showed that this seedless cultivar (Ngumpen) was grouped in to different cluster, a part from others. In comparison with 4 seeded cultivars of wani, Ngumpen cultivar had a similar nutrient content, however, it had greater fiber and a greater percentage of edible part. We concluded that the Ngumpen cultivar was a specific and unique germplasm so that should be preserved and protected.
Pengaruh Konsentrasi Antitranspiran Chitosan Terhadap Pembuahan dan Produksi Salak Gula Pasir di Luar Musim SUNARKA, I KETUT; RAI, I NYOMAN; KARTINI, NI LUH
Agrotrop: Journal on Agriculture Science Vol 5, No 1 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

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The Effect of Antitranspirant Chitosan Consentrantions to Fruit-Set and Production ff Salacca zalacca var. Gula Pasir on Off-Season. Salak Gula Pasir (Salacca zalacca var. Gula Pasir) naturally flowering once every three months or four times a year. During the four times flowering, the best harvest or fruit production only once a year while the other three flowering are failed become a fruit-set. The failure was caused by rainfall and low rainy time. This research aimed to know the effect of several Antitranspirant Chitosan Consentrations to the success of flower to become a fruit (fruit-set) on Salacca zalacca var. Gula Pasir. This research used random sampling where the area was divided into four Antitranspirant Chitosan Consentrations. The factor of Antitranspirant Chitosan Consentrations consists of four levels (0%, 15%, 30%, and 45%). The research was conducted at the central production of Salacca zalacca var. Gula Pasir in Sibetan Village, Bebandem District, Karangasem Regency. The result of giving Antitranspirant Chitosan Consentrations at 45% was effective improved the success of flower to become a fruit-set of 64,48% on Gadu Season while on Sela II Season it effective improved 84,38% at the 30% consentrations.
Mengatasi Kegagalan Fruit-Set dengan Pembuangan Bekas Tandan Bunga dan Anakan Pada Salak Gula Pasir (Salacca zalacca var. Gula Pasir) SAPUTRA, I WAYAN EKA; WIRAATMAJA, I WAYAN; RAI, I NYOMAN
Agrotrop: Journal on Agriculture Science Vol 5, No 1 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Overcoming the Failure of Fruit-set by Removing Former Flower Stem and Shoot on Salak Gula Pasir Fruit (Salacca zalacca var. Gula Pasir). This research aimed to overcome the failure of fruit-set by removing former flower bunch and shoot on salak gula pasir (Salacca zalacca var. Gula Pasir). This research was a factorial experiment, designed using randomized completely block design with 2 factors. The first factor was removal of former flower bunch, consisted of 2 levels (removed and not removed), and the second factor was removal of shoot, consisted of 2 levels (removed and not removed). Thereby, there were 4 treatments combination and replicated 7 times so that 28 plants was required. The result of the research showed that the greatest percentage of fruit-set (80,15%) was obtained on the shoot removal treatment, whereas the fruit-set of the treatment on which shoot was not removed was 60,34%. On the removal of former flower bunch treatment, there was no significant difference on removal and not removal treatment between the percentages of fruit-set.