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ISOLATION AND IDENTIFICATION OF MICROORGANISMS DURING SPONTANEOUS FERMENTATION OF MAIZE [Isolasi dan Identifikasi Mikroorganisme pada Fermentasi Spontan Jagung] Rahmawati, .; -Hariyadi, Ratih Dewanti; Hariyadi, Purwiyatno; Fardiaz, Dedi; Richana, Nur
Jurnal Teknologi Dan Industri Pangan Vol 24, No 1 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

ISOLATION AND IDENTIFICATION OF MICROORGANISMS DURING SPONTANEOUS FERMENTATION OF MAIZE [Isolasi dan Identifikasi Mikroorganisme pada Fermentasi Spontan Jagung] Rahmawati1,2), Ratih Dewanti-Hariyadi1,3)*, Purwiyatno Hariyadi1,3), Dedi Fardiaz1,3) and Nur Richana4) 1) Department of Food Science and Technology, Bogor Agricultural University, Kampus IPB Darmaga, Bogor, Indonesia 2) Department of Food Technology, Sahid University, Jakarta, Indonesia 3) Southeast Asia Food Agricultural Science and Technology (SEAFAST) Center, Bogor Agricultural University, Kampus IPB Darmaga, Bogor, Indonesia 4) Indonesian Center for Agricultural Post Harvest Research & Development (ICAPRD), Bogor, Indonesia   Accepted April 05th 2013 / Approved June 12th  2013 ABSTRACT   Maize was traditionally the second most common staple food in Indonesia. Conversion to maize flour has been accomplished to improve its convenience. Traditionally, maize flour is produced by soaking the kernels in water followed by grinding. It was reported that final physicochemical characteristics of the maize flour were influenced by spontaneous fermentation which occurred during soaking. This research aimed to isolate and identify important microorganisms that grew during fermentation thus a standardized starter culture can be developed for a more controlled fermentation process. Soaking of maize grits was conducted in sterile water (grits:water=1:2, w/v) in a closed container at room temperature (±28ºC) for 72 hours. After 0, 4, 12, 24, 36, 48, 72 hours, water and maize grits were sampled and tested for the presence of mold, yeast, and lactic acid bacteria (LAB). Isolates obtained from the spontaneous fermentation were reinoculated into the appropriate media containing starch to observe their amylolytic activity. Individual isolate was then identified; mold by slide culture method, while yeast and LAB by biochemical rapid kits, i.e. API 20C AUX and API CH50, respectively. The number of each microorganism was plotted against time to obtain the growth curve of the microorganisms during spontaneous fermentation. The microorganisms were identified as Penicillium chrysogenum, P. citrinum, A. flavus, A. niger, Rhizopus stolonifer, R.oryzae, Fusarium oxysporum, Acremonium strictum, Candida famata, Kodamaea ohmeri, Candida krusei/incospicua, Lactobacillus plantarum 1a, Pediococcus pentosaceus, L. brevis 1, L. plantarum 1b, and L. paracasei ssp paracasei 3. Four molds and one yeast were amylolytic while none of the LAB was capable of starch hydrolysis. The growth curve suggested that the amylolitic mold and yeast grew to hydrolyze starch during the course of fermentation, while the LABs benefited from the hydrolyzed products and dominated the later stage of the fermentation.  
POLA MAKAN DAN AKTIFITAS FISIK DENGAN KADAR GLUKOSA DARAH PENDERITA DIABETES MELLITUS TIPE 2 RAWAT JALAN DI RSUP Dr. WAHIDIN SUDIROHUSODO MAKASSAR Rahmawati, .
MEDIA GIZI MASYARAKAT INDONESIA Vol 1, No 1 (2011)
Publisher : Fakultas Kesehatan Masyarakat UNHAS

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Abstract

The high increasing of diabetes mellitus associated with lifestyle changes, the most prominent factors are changes in dietary patterns of all instant, high-fat, contain lots of sugar, and plus the lacking of physical activity. The aim of this  study was to determine relationship of dietary pattern and physical activity with blood glucose levels in people with type 2 diabetes mellitus outpatient in Poly Endocrine RSUP Dr. WahidinSudirohusodo Makassar. The type of this study was a descriptive-analytic with cross-sectional study design. The sampling was done by using purposive sampling technique, there were 108 outpatients was selected  as samples but  only  81 people who meet the criteria. Data collection was done by direct interviews, the results of lain boratory, and the results clinical. The data analysis was performed by using chi-square test (p = 0,05). The result indicated that dietary (risk of dietary habit) and (food quality) had a significant association with blood glucose levels of people that suffer from type 2 DM  (p = 0,046) and (p = 0,001) means that p<α (0,05). As for the physical activity variable  was found that there was a significant association too, with blood glucose levels in people with type 2 DM  (p = 0,003) means that p<α (0,05). From this research, it was recommended to the patients to regard of meal intake that trigger to increase the blood glucose levels, encouraged to do regular exercise activities at least 3 times per week, and reduce sedentary life. Keywords : glucose, dietary pattern, physical activities
PENINGKATAN KEMAMPUAN MEMBACA PERMULAAN MELALUI MEDIA KARTU KATA PADA KELOMPOK USIA 5 – 6 TAHUN DI PAUD Rahmawati, .; ., Fadilah; Astuti, Indri
Jurnal Pendidikan dan Pembelajaran Vol 3, No 10 (2014): Oktober 2014
Publisher : Jurnal Pendidikan dan Pembelajaran

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Abstract

Abstrak : Penelitian ini bertujuan untuk meningkatkan kemampuan membaca permulaan  melalui media kartu kata pada Kelompok  Usia 5 - 6 Tahun di PAUD Laskar Pelangi  Kabupaten Kubu Raya. Metode penelitian yang digunakan adalah Penelitian Tindakan Kelas dengan metode sentra pembelajaran, sentra yang peneliti gunakan adalah sentra persiapan. Subjek penelitian ini adalah berjumlah 28 orang anak  terdiri dari 14 laki-laki dan 14 perempuan. Hasil analisis data menunjukan bahwa terdapat peningkatan secara umum pada perencanaan pembelajaran dalam Peningkatan Kemampuan  Membaca Permulaan Melalui Media Kartu Kata Pada Kelompok  Usia 5 – 6  Tahun Di Paud Laskar Pelangi  Kabupaten Kubu Raya, Namun disarankan kepada guru-guru sebaiknya mengajarkan membaca permulaan sebaiknya dilakukan dengan cara menyenangkan dan disesuaikan berdasarkan tahapan  umur atau kemampuan anak  sehingga anak menjalaninya dalam keadaan senang dan tidak tertekan. Kata kunci : membaca, metode, kartu kata Abstract : This study aims to improve the reading skills through the medium of flash cards beginning at age group 5-6 years in early childhood Rainbow Warriors Kubu Raya district. The research method used was Classroom Action Research method of learning centers, centers that researchers use is the center of preparation. The subjects were 28 in total consists of 14 children boy and 14 girl. The results of data analysis showed that there was a general increase in the learning plan in Beginning Reading Upgrades Through Media flash cards In Age Group 5-6 Years In early childhood Rainbow Warriors Kubu Raya District, however advised the teachers should teach beginning reading should be done by fun and adjusted based on the stage of the age or ability of the child so that the child live in a state of happy and not depressed. Keyword  : reading, method, flash cards
Implementation of Public Private Partnership in The Management Market RAU (Rau Trade Center) In Serang City Rahmawati, .
International Multidiciplinary Conference on Social Sciences (IMCoSS) Vol 1 (2015): 3rd IMCoSS 2015
Publisher : Bandar Lampung University

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Abstract

According to the approach of New Public Service, the governments main focus is public service. As an institution in charge of providing services to the public, the government certainly can not do it all by itself or single fighter. This is because many types of services that must be provided to the public. Therefore do a cooperation contract between the government and the private sector (KSP) in supplying and servicing the public. Memorandum of understanding contract between the government and private or Public Private Partnership (PPPs) considered very necessary to organize public services, as well as the procurement and management of Rau Market (Rau Trade Center). Besides as icons Kota Serang, Rau Trade Center also serves as a tool in the development of the local economy. This study uses the theory of policy implementation according to Charles O. Jones, namely the organization, interpretation and application. The method used is qualitative case study approach. The results showed that the form of cooperation between the government of Kota Serang with PT. Pesona Banten Persada in the management of rau market is Build Operate Transfers with a concession period of 20 years since 2002. The cooperation is carried out to increase the economic activity of society to accommodate many traders. Rau market management differentiated by region in the building is the responsibility of the private sector and the authorities covering the costs of lease / sale price rental kiosks and stalls, hygiene management of garbage and cleanliness and parking levy withdrawal. Outside the building into the management of local authorities in terms of cleanliness and parking levies, while for rental kiosk / los beyond the authority of the local government because it is owned by an individual. Contributions partnership on revenue of Serang not optimal, amounting to 300 million per month from the levy cleanliness and parking. There has been no further decisions regarding the management of rau by the private market will continue or will be submitted to the local government after the concession period ends.
USING READ, ENCODE, ANNOTATE AND PONDER (REAP) STRATEGY IN TEACHING READING COMPREHENSION Rahmawati, .; Supardi, Iwan; Bunau, Eusabinus
Jurnal Pendidikan dan Pembelajaran Vol 4, No 6 (2015): JUNI 2015
Publisher : Jurnal Pendidikan dan Pembelajaran

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Abstrak: Penelitian ini bertujuan untuk mengetahui apakah strategi REAP memberikan hasil belajar yang berbeda atau tidak dalam pengajaran pemahaman membaca pada teks naratif untuk  siswa kelas  sebelas MAN Mempawah. Penelitian ini juga dilakukan untuk mengetahui keefektifan penerapan strategi REAP untuk pengajaran pemahaman membaca. Experimen semu dengan rancangan kelompok non-equivalent digunakan dalam penelitian ini. Sample penelitian ini adalah siswa dari kelas XI Ilmu Sosial 1 sebagai kelompok eksperimen dan  siswa dari kelas XI Ilmu Sosial 2 sebagai kelompok kontrol. Hasil analisis data menunjukan bahwa hasil belajar siswa dalam pemahaman membaca berbeda setelah menerapkan strategi REAP. Hal ini di tunjukan oleh selisih skor antara pretest and posttest pada kelompok eksperimen, yaitu sebesar (11.59), lebih tinggi dari selisih skor antara pretest dan posttest pada kelompok kontrol, yaitu sebesar (6.37). Kefektifan strategi ini juga ditunjukan oleh efek size formula ( 0.98, yang tergolong mempunyai efek yang sedang. Kata Kunci : Membaca, Teks Naratif, Strategi REAP Abstract: This research aims to find out whether REAP strategy gives difference learning achievement or not for teaching reading comprehension on narrative text to the eleventh grade students of MAN Mempawah. It was also to know the effectiveness of using REAP Strategy for teaching reading comprehension. A quasi experimental with non-equivalent group design is used. The samples of this research were students of XI Social Sciences class 1 as experimental group and others of XI Social Sciences class 2 as the control group.  The result of data analysis showed that the students’ achievements in reading comprehension are different after using REAP strategy. It showed by the interval score between the pretest and posttest in experimental group (11.59) was higher than the interval score between pretest and posttest in control group (6.37). This strategy was also proved effective, showed by the effect size value ( 0.98, qualified as having moderate effect.   Key Words: Reading, Narrative Text, REAP Strategy
Hygiene, sanitation and the soil transmitted helminths (STH) infection among elementary school students in West Lombok Rahmawati, .; Soeyoko, .; Sumarni, Sri
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 02 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

The prevalenses of soil transmitted helminths (STH) infection in Lombok are relatively high. It isclosley associated with poor environmetal hygiene, non healthy sanitation status, poverty andimproper health services. The highest prevalences is normally observed in elementary school children.The aim of this study was to evalaute the relationship between hygiene, sanitation and STH infectionamong elementary school children in West Lombok. An observational study with cross-sectionaldesign was performed involving 197 elementary school children in Terong Tawah Village, LabuapiSub District, West Lombok District and 197 children in Ampenan, Mataram City as control. Thestool specimen of the children were collected and processed using Kato-Katz technique. Hygieneand sanitation of each subject that consisted nail hygiene, hand washing, using footwear, latrine,clean water availability, house floor, soil around the house were taken by questionnaire and directobservation. The result showed that the prevalences of STH among elementary school children inTerong Tawah (81.7%) was higher than that in Ampenan (12.7%).Trichuris trichiurawas thepredominant helminth both in Terong Tawah (36.0%) and Ampenan (7.0%) followed byA.lumbricoides(20.4% in Terong Tawah and 4.5% in Ampenan). The mix infections betweenT.trichiuraandA. lumbricoidesamong the children in Terong Tawah (24.4%) and Ampenan (1.0%)were also found. A significant relationship between hygiene and sanitaion i.e. nail cleaniness, washinghands, contaminated soil around the house and STH infection among elementary school children inTerong Tawah and Ampenan (p<0.05). In conclusion,there is a significant relationship betweenpersonal hygiene, environmental sanitation and STH infections among elementary school children.
ISOLATION AND IDENTIFICATION OF MICROORGANISMS DURING SPONTANEOUS FERMENTATION OF MAIZE [Isolasi dan Identifikasi Mikroorganisme pada Fermentasi Spontan Jagung] Rahmawati, .; -Hariyadi, Ratih Dewanti; Hariyadi, Purwiyatno; Fardiaz, Dedi; Richana, Nur
Jurnal Teknologi dan Industri Pangan Vol 24, No 1 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Maize was traditionally the second most common staple food in Indonesia. Conversion to maize flour has been accomplished to improve its convenience. Traditionally, maize flour is produced by soaking the kernels in water followed by grinding. It was reported that final physicochemical characteristics of the maize flour were influenced by spontaneous fermentation which occurred during soaking. This research aimed to isolate and identify important microorganisms that grew during fermentation thus a standardized starter culture can be developed for a more controlled fermentation process. Soaking of maize grits was conducted in sterile water (grits:water=1:2, w/v) in a closed container at room temperature (?28?C) for 72 hours. After 0, 4, 12, 24, 36, 48, 72 hours, water and maize grits were sampled and tested for the presence of mold, yeast, and lactic acid bacteria (LAB). Isolates obtained from the spontaneous fermentation were reinoculated into the appropriate media containing starch to observe their amylolytic activity. Individual isolate was then identified; mold by slide culture method, while yeast and LAB by biochemical rapid kits, i.e. API 20C AUX and API CH50, respectively. The number of each microorganism was plotted against time to obtain the growth curve of the microorganisms during spontaneous fermentation. The microorganisms were identified as Penicillium chrysogenum, P. citrinum, A. flavus, A. niger, Rhizopus stolonifer, R.oryzae, Fusarium oxysporum, Acremonium strictum, Candida famata, Kodamaea ohmeri, Candida krusei/incospicua, Lactobacillus plantarum 1a, Pediococcus pentosaceus, L. brevis 1, L. plantarum 1b, and L. paracasei ssp paracasei 3. Four molds and one yeast were amylolytic while none of the LAB was capable of starch hydrolysis. The growth curve suggested that the amylolitic mold and yeast grew to hydrolyze starch during the course of fermentation, while the LABs benefited from the hydrolyzed products and dominated the later stage of the fermentation.
Hubungan Kekerabatan Fenetik Tujuh Anggota Familia Apocynaceae Rahmawati, .
Jurnal Ilmiah Mahasiswa Pendidikan Biologi Vol 1, No 1 (2016): Agustus 2016
Publisher : Jurnal Ilmiah Mahasiswa Pendidikan Biologi

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Abstract

Penelitian berjudul “Hubungan Kekerabatan Fenetik Tujuh Anggota Familia Apocynaceae” telah dilaksanakan pada tanggal 22 s.d 29 Februari 2016. Tujuan penelitian untuk mengetahui hubungan kekerabatan fenetik tujuh anggota familia Apocynaceae. Metode penelitian yang digunakan dalam penelitian ini merupakan metode deskriptif. Sampel dalam penelitian ini tujuh spesies anggota familia Apocynaceae antara lain yaitu Allamanda cathartica, Plumeria acuminata, Alstonia scholaris, Catharanthus roseus, Nerium oleander, Cerbera manghas, dan Thevetia peruviana. Parameter yang digunakan yaitu ciri morfologi batang, daun, bunga, buah dan biji dan jenis habitus. Analisis data melalui perhitungan Indeks Similaritas (IS). Selanjutnya dilakukan Analisis Cluster untuk mengelompokkan tanaman yang memiliki kesamaan karakteristik dari tujuh spesies. Hasil penelitian menunjukkan bahwa kekerabatan sangat dekat terdapat pada kombinasi spesies Cerbera manghas dan Thevetia peruviana dengan nilai IS= 0,82. Adapun kekerabatan dekat terdapat pada 11 kombinasi spesies dengan nilai indek similaritas berkisar antara IS= 0,71 - 0,53. Sebaliknya Kekerabatan tidak dekat terdapat pada 9 kombinasi spesies dengan nilai Indeks Similaritas berkisar antara IS= 0,50 - 0,38. Kesimpulannya adalah hubungan kekerabatan pada 7 spesies anggota familia Apocynaceae terdapat tiga katagori kekerabatan yaitu kekerabatan sangat dekat, dekat, dan tidak dekat. Kekerabatan sangat dekat ditunjukkan pada kombinasi spesies Cerbera manghas dan Thevetia peruviana dengan Indeks Similaritas tertinggi yaitu 0,82. Sedangkan hubungan kekerabatan yang jauh ditunjukkan pada kombinasi spesies Allamanda cathartica dan Nerium oleander dengan nilai IS= 0,38.
SEX RATIO ANAK PUYUH (Coturnix-coturnix japonica) PADA TETUA YANG DIBERI RANSUM TERSUPLEMENTASI MINERAL Zn DAN VITAMIN E Rahmawati, .
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 3, No 2 (2016): JITRO, Mei
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

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Abstract

           Jenis kelamin unggas dapat dimanipulasi, yaitu melalui pengubahan jumlah hormon estrogen dan testosteron di dalam tubuh dan yolk.  Pengubahan ini dapat dilakukan melalui pengaturan kandungan Zn pada pakan.  Selain Zn yang dapat mempengaruhi fungsi reproduksi ternak, vitamin E juga  bermanfaat untuk meningkatkan fertilitas, pertumbuhan embrio normal dan sebagai antioksidan.  Penelitian bertujuan untuk mengkaji pengaruh suplemen mineral Zn, vitamin E dan interaksi keduanya terhadap sex ratio anak puyuh.  Penelitian menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 3 x 3 dengan 3 ulangan.  Faktor pertama adalah level mineral Zn (0mg, 50mg dan 100mg) ) dan faktor kedua adalah level vitamin E (0ppm, 45ppm dan 50ppm).  Setiap unit percobaan terdiri dari 2 ekor puyuh betina dan 1 ekor puyuh jantan. Parameter yang diukur adalah  konsumsi ransum,  bobot telur, daya tetas dan sex ratio anak puyuh. Hasil penelitian menunjukkan bahwa Interaksi antara mineral Zn dan vitamin E berpengaruh terhadap bobot telur, namun tidak berpengaruh terhadap konsumsi ransum, daya tetas dan sex ratio anak puyuh. Penambahan mineral Zn berpengaruh nyata terhadap bobot telur, namun tidak mempengaruhi konsumsi ransum, daya tetas dan sex ratio anak puyuh.  Penambahan vitamin E berpengaruh nyata terhadap konsumsi ransum, bobot telur dan daya tetas, namun tidak berpengaruh terhadap sex ratio anak puyuh. Kata kunci : Anak puyuh, mineral Zn, vitamin E, sex ratio. Abstract
PENGARUH PDB DAN IMPOR TERHADAP SEABORNE TRADE (Studi Pada Seaborne Trade Jenis General Cargo Containerized Indonesia Tahun 2001-2013) Rahmawati, .; Al Musadieq, Mochammad; Supriono, .
Jurnal Administrasi Bisnis Vol 38, No 1 (2016): SEPTEMBER
Publisher : Fakultas Ilmu Administrasi Universitas Brawijaya

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This study aims to determine the effect of GDP and imports Against General Cargo Contanerized Indonesia. The independent variable in this research is the GDP and Importswith the dependent variable is General Cargo Contanerized Indonesia.Type of this research is an explanatory research with quantitative approach.Focusing searching Indonesia General Cargo Containerized in 2001 up to 2013.Methods of data collection is by documentation of data from the Central Bureau of Statistics (BPS) and World Bank website. Method of data analysis is descriptive analysis and multiple linear regression performed classical assumption previously.F test results showed that GDP and imports were significant effect on the General Cargo Containerized Indonesia. The t-test showed that GDP had positive influence and no significant on the General Cargo Containerized Indonesia andImports have a positive and significant impact on the General Cargo Containerized Indonesia. Keywords : GDP, Imports, Seaborne Trade, General Cargo Containerized, Container Throughput. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh PDB dan impor Terhadap General Cargo Contanerized Indonesia. Variabel bebas dalam penelitian ini adalah PDB dan Import dengan variable dependen General Cargo Contanerized Indonesia. Jenis penelitian ini merupakan penelitian penjelasan dengan pendekatan kuantitatif. Fokus penelitian pada General Cargo Containerized Indonesia pada tahun 2001 sampai dengan 2013. Metode pengumpulan data adalah dengan dokumentasi data dari website Badan Pusat Statistik (BPS) dan Bank Dunia. Metode analisis data adalah analisis deskriptif dan regresi linier berganda dengan dilakukan asumsi klasik sebelumnya. Hasil uji F menunjukkan bahwa PDB dan impor berpengaruh signifikan dari General Cargo Containerized Indonesia. T-test menunjukkan bahwa PDB memiliki pengaruh positif dan tidak ada yang signifikan dari General Cargo Containerized Indonesia dan Impor memiliki dampak positif dan signifikan dariGeneral Cargo Containerized Indonesia. Kata Kunci : PDB, Impor, Seaborne Trade, General Cargo Containerized, Container Throughput.