Articles

Found 3 Documents
Search

MODIFIKASI SINTESIS NUKLEOTIDA BERTANDA [γ-32 P] ATP

Jurnal Radioisotop dan Radiofarmaka Vol 16, No 1 (2013): JURNAL PRR 2013
Publisher : Jurnal Radioisotop dan Radiofarmaka

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1248.646 KB)

Abstract

ABSTRAKMODIFIKASI SINTESIS NUKLEOTIDA BERTANDA [Y-32P]ATP.Dalam perkembangan biologimolekul, radionuklida dalam bentuk senyawa bertanda telah digunakan sebagai perunut deoxyribonucleicacid (DNA)/ribonucleic acid (RNA) untuk mendalami berbagai macam proses fisiologi dan patologi. Salahsatu senyawa tersebut adalah nukleotida bertanda fosfor-32 (32P) [γ-32P]-adenosine triphosphate {[γ-32P]-ATP} yang banyak digunakan dalam penelitian biologi molekul. Untuk dapat menunjang penelitian biologimolekul di Indonesia, pada penelitian ini telah dilakukan pembuatan senyawa nukleotida bertanda [γ-32P]-ATP melalui reaksi enzimatis dengan melakukan modifikasi pada metoda sintesisnya menggunakanprekursor DL-glyceraldehyde 3-phosphate, nukleotida adenosine di-phosphate (ADP) dan H332PO4, sertaenzim gliseraldehid 3-phosphat dehidrogense, 3-phosphogliserat-kinase dan laktat dehidrogenase. Pemurnian[γ-32P]-ATP hasil sintesis dengan menggunakan kolom kromatografi DEAE-Sephadex. Dari proses sintesisdan pemurnian yang telah dilakukan berhasil diperoleh [γ-32P]-ATP dengan aktifitas 1,175 mCi dankemurnian radiokimia 99,49%. Dengan berhasilnya dilakukan sintesis dan pemurnian [γ-32P]-ATP, makaPusat Radiosiotop dan Radiofarmaka akan dapat menyediakan nukleotida bertanda dimaksud di atas untukmenunjang penelitian biologi molekul di Indonesia.Kata Kunci: nukleotida bertanda [γ-32P]ATP, sintesis, reaksi enzimatis, pemurnianABSTRACTMODIFICATION OF SYNTHESIS NUCLEOTIDES [Y-32P] ATP.In molecular biology, radionuclidesin the form of radiolabeled compounds have been widely used as deoxyribonucleic acid (DNA) / ribonucleicacid (RNA) tracer in order to explore a wide range of physiological and pathological processes. One of suchcompounds is [γ-32P]-adenosine triphosphate {[γ-32P]-ATP} [γ-32P]-ATP which has been widely used in thebiotechnology research. In order to support the biotechnology research in Indonesia in this project, [γ-32P]-ATP had been synthesized by enzymatic reactions with modifying the method of synthesis using theprecursor DL-glyceraldehydde 3-phosphate, nucleotides Adenosine Diphosphate (ADP) and H332PO4 andenzymes glyceraldehid 3-phosphate dehydrogenase, 3-phosphoglyceryc phosphokinase and lactatedehydrogenase. The purification of the synthesized [γ-32P]-ATP, by using DEAE Sephadex columnchromatography.  The synthesis and purification process that had been performed were able in producing of[γ-32P]-ATP with radioactivity of 1,175 mCi and  radiochemical purity of 99,49%.. Having successfullyprepared the [γ-32P]-ATP and application, in the near future the Radioiotopes and RadiopharmaceuticalsCentre is expected to be able in providing the above-mentioned radiolabeled nucleotide for biotechnologyresearch in Indonesia.Key words : labeled nucleotide [γ-32P]-ATP, synthesis, enzimatic reaction, purification

Preliminary Study on Production Of 32P – Labeled Phosphate Chromic as A Material for Skin Patch

Indonesian Journal of Pharmaceutical Science and Technology Suppl. 1, No. 3 (2019)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Original Source | Check in Google Scholar

Abstract

Keloids are skin disorders or benign tumours that are due to abnormal wound healing in the binding tissue after a trauma, inflammation, surgical wounds, or burns. Low activity radioisotopes have shown to be effective in curing or eliminating keloids on the skin. One of these radioisotopes is phosphorus-32 (32P), a beta (β-) emitter with a half-life of 14.3 days. This radioisotope can also be developed for the treatment of keloid and skin tumours. Currently, keloid is treated by a conventional method e.g.by applying the bulk of 32P radioisotope directly on keloid area. However, this method is considered inefficient and less secure. The purpose of this research is to obtain a technology for preparing of 32P-labeled skin patch. The first step of this research is to produce 32P-labeled chromic phosphate (Cr32PO4) colloids, through condensation involving oxidation-reduction reaction. In this step, Cr (VI) is reduced to Cr (III) to form Cr32PO4 with particle size of <1 μm.  These particles (Cr32PO4) are to expect to distribute evenly when mixed with silicon to form skin patch which will not decompose easily. Characterization of the prepared Cr32PO4colloids gave a yield of 97,8% with particle size of greater than > 1μm.  Further study needs to be performed in due time in order to have Cr32PO4 colloids with a suitable particle size.Key words: keloid, chromic phosphate colloid, skin patch, condensation, oxidation-reduction reaction

Studi Awal Pembuatan Koloid Kromik Fosfat Bertanda Radioisotop 32P Sebagai Bahan Pembuatan Skin Patch

Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2019: PROSIDING SNTKK 2019
Publisher : Prosiding Seminar Nasional Teknik Kimia "Kejuangan"

Show Abstract | Original Source | Check in Google Scholar

Abstract

Keloids are skin disorders or benign tumors that are due to abnormal wound healing in the binding tissue after a trauma, inflammation, surgical wounds, or burns. Low activity radioisotopes have shown to be effective in curing or eliminating keloids on the skin. One of these radioisotopes is phosphorus-32 (32P), a beta (β-) emitter with a half-life of 14.3 days. This radioisotope can also be developed for the treatment of keloid and skin tumors. Currently, keloid is treated by a conventional method e.g. by applying the bulk of 32P radioisotope directly on keloid area and this method is considered inefficient and less secure. The purpose of this research is to obtain technology for preparing of a 32P-labeled skin patch. The first step of this research is to produce 32P-labeled chromic phosphate (Cr32PO4) colloids, through condensation involving oxidation-reduction reaction. In this step, Cr (VI) is reduced to Cr (III) to form Cr32PO4 with a particle size of <1 μm.  These particles (Cr32PO4) are to expect to distribute evenly when mixed with silicon to form skin patch which will not decompose easily.  Characterization of the prepared Cr32PO4 colloids gave a yield of 97,8%. Geometric standard deviation (sg) of colloidal particles amounted to 163.7 nm shaped poly-disperse.  Further study needs to be performed in due time in order to have Cr32PO4 colloids with suitable particle size.