Mohammad Zen Rahfiludin
FKM UNDIP Semarang

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Journal : Media Gizi Mikro Indonesia

Pengaruh Suplementasi Seng terhadap CD 4+ Pengidap Human Immunodeficiency Virus

Media Gizi Mikro Indonesia Vol 5, No 1 (2013)
Publisher : Media Gizi Mikro Indonesia

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Abstract

ABSTRACT Optimal immune response is needed to viral elimination, but in HIV infection the immune cell is the main target. Zinc has proved to increase immune response to various infection. However, its role in HIV infection has not understood. This study aimed to analyze the effect of zinc supplementation to increase CD4+ in HIV-infected patients. This was an experimental study with pre test post test with control group design. Twenty HIV-infected persons were devided into 2 groups: control group which received Anti Retroviral Therapy (AZT) and the Zn+ART group received 5 mg zinc/d orally for one month and AZT. Field workers visited patients for checking compliance. Venous blood was taken from all of the subjects, the CD4+ cell count was measured and daily nutrient intake was analyzed. CD4+ cell count was determined by flowcytometri method. Daily food intake was determined by two 24 hour recall periods during 2 non consecutive days. Differential test between the two groups was performed using independent t-test or Mann Whitney test, when the distribution was not normal. Analysis of CD4+ differences before and after treatment in each group by paired t-test. The mean of CD4+ before zinc supplementation was 371.3 ± 126.8 cell/μL and increase to 415.2 ± 194.1 cell/μL after supplementation. However the increase 43.9 ± 83.5 cell/μL was not significantly different (p= 0.131). There was not a significant change CD4+ (p= 0.112) between both groups, possibly because the dose and duration of zinc supplementation. Moreover, only one type of micronutrient given (zinc), also led to an increase CD4+ in our study did not significantly. Zinc supplementation in complement with AZT therapy was not significantly increase CD4+ in HIV patients. Keywords: zinc supplementation, CD4+, HIV, AZT.   ABSTRAK Respon imun yang optimal diperlukan untuk pemusnahan virus, namun dalam infeksi HIV justru sel sistem imun yang diserang. Seng telah terbukti dapat meningkatkan respon imun terhadap berbagai infeksi, namun belum banyak diketahui perannya terhadap infeksi HIV. Tujuan penelitian untuk menganalisis efek suplementasi seng terhadap CD4+ pengidap HIV. Penelitian ini merupakan studi eksperimen dengan pre test post test control group design. Dua puluh orang pengidap HIV ikut dalam penelitian ini, dibagi menjadi dua kelompok. Sepuluh orang menerima azidothymidine (AZT) ditambah suplementasi seng dengan dosis 5 mg seng/hari selama satu bulan, sepuluh orang memperoleh AZT saja. Kepatuhan mengkonsumsi AZT dan suplementasi seng dicatat dan disupervisi oleh petugas lapangan. Semua sampel diambil darah venanya, diperiksa CD4+ dan dianalisis masukan zat gizi hariannya. Pengukuran CD4+ dilakukan dengan metode flowcytometri. Data asupan makanan harian dikumpulkan dengan metode recall 2 X 24 jam tidak berturutan harinya. Uji beda antara kedua kelompok