Articles

KOMBINASI OBAT ANTITUBERKULOSIS PADA PASIEN ANAK RAWAT JALAN ASKES DI RSUD Prof. Dr. MARGONO SOEKARJO

PHARMACY Vol 5, No 1 (2007)
Publisher : PHARMACY

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Abstract

Telah dilakukan penelitian tentang kombinasi obat antituberkulosis yang paling banyakdigunakan pada pasien anak rawat jalan Askes di RSUD Prof. Dr. Margono SoekarjoPurwokerto. Tujuan penelitian ini adalah untuk mengetahui apakah kombinasi obat yang palingbanyak digunakan tersebut berinteraksi. Data kombinasi obat diteliti dengan mengamati rekanmedik pasien anak rawat jalan Askes. Data yang diambil meliputi pengamatan resep, tabulasidata yang kemudian dianalisis secara deskriptif non analitik. Hasil analisis menunjukkan bahwakombinasi obat antituberkulosis yang paling banyak digunakan adalah isoniazida dan rifampisin.Pasien yang diteliti sebanyak 179 pasien. 87,71% pasien potensial terjadi interaksi obat yangsignifikan secara klinis, 49,04% mengalami kenaikan SGPT dan atau perbandingan SGOTterhadap SGPT akibat kombinasi obat, 1,29% manifes dan 1,29% meninggal. Dari hasil tersebutternyata isoniazida dan rifamfisin mempunyai efek samping kombinasi obat hepatotoksik.Kata kunci: obat anti tuberkulosis, kombinasi obat antituberkulosis, pasien anak rawat jalan,RSUD Prof. Dr. Margono Soekarjo

PENGARUH KONSENTRASI DAN LAMA PERENDAMAN DALAM SUPERNATAN KULTUR Bacillus sp.2 DUCC-BR-KI.3 TERHADAP PERTUMBUHAN STEK HORISONTAL BATANG JARAK PAGAR (Jatropha curcas L.)

JURNAL SAINS DAN MATEMATIKA Volume 17 Issue 3 Year 2009
Publisher : JURNAL SAINS DAN MATEMATIKA

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ABSTRACT--Jarak pagar (J. curcas L.) is a plant whose the seed can produce oil or it is known as biodiesel. J.  curcass oil is the source of alternative oil energti that can be renewable and environmentally safe. Another ways  to increase the production of J. curcass oil is by improving its  productivity. This activity cannot be separated from the problem of supplying the seed. Stem cutting  is one of the most ways to provide the seed than another way. The growth of stem cutting can be stimulated by giving ZPT that is IAA both naturally and synthetically. Bacillus  sp.2  DUCC-BR-KL.3 supernatant culture that contain IAA can be one of naturally alternative hormone. The aims of this research was to study the effect of concentration and submersion period in Bacillus  sp.2 DUCC-BR-KL.3  supernatant culture to the growth of J. curcas L. hoizontal stem cutting. This experiment was  condueted in Plants Biologi Structure and Function Laboratory, MIPA faculty, Diponegoro University. Experimental design used in this research is Completely Random  Design with Factorial pattern 5x4 by using 5 replications. The first factor was concentration, ie P0 (control), P1 (25%), P2 (50%), P3 (75%) and P4 (100%). The  second factor was submerged periods, i.e T1 (1 hour), T2 (2 hours), and T3 (3 hours). The data were analyzed by using Kruskal-Wallis Test and ANOVA, if there are significantly  influence, were analyzed by using Duncan multiple range test with 95% confidence intevals that is in the variable of root lenght and weight of dry root. The result of this sudy showed that the giving of Bacillus sp.2 DUCC-BR-KL.3 supernatant culture influence to improve the bud and root growth of J. curcas L. Keywords :  Jatropha culcos L., supernalant, concentration, growth

MIX CULTURE INOCULANT PRODUCTION OF PHOSPHATE SOLUBILYZING AND INDOLE ACETIC ACID (IAA) PRODUCER RHIZOBACTERIA WITH AMBARAWA PEAT SOIL RAWAPENING AS CARRIER

JURNAL SAINS DAN MATEMATIKA Volume 18 Issue 2 Year 2010
Publisher : JURNAL SAINS DAN MATEMATIKA

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ABSTRACT---Plant production development  is the main goals  that do for increase the farming quality to fulfill the man needed  in  food. One of the ways is intensive farming, by using organic or inorganic  fertilizer. Phosphate is the essential  for plants. IAA is the necessary plant regulator for the root. Both phosphate  and IAA need in  plant  growing  and production.  Biological  fertilizer  is  fertilizer  with  microbial  as the main material. Bacillus sp.DUCC-BR-K1.7, Bacillus sp. DUCC-BR-KI3, Bacillus sp.DUCC-BR-KI.2b  and Pseudomonas fluorescens, Azotobacter chroococcum, Azotobqcter vinelandii, Azospirillum sp. and Azotobacter brazilensis are bacteria that can solubilization  the phosphate  and IAA  synthesize.  Those bacteria can be used as  inoculants or biological  fertilizer  that put on carrier. One way to support  the aim is giving  the altemative carrier with  suitable composition. The carrier should be support bacterial life  during the storage.  The aim ofthis  research  is find  ths right  consortia so can be used to optimized  viabilities  of  culture Bacillus  sp. DUCC-BR-KIJ,  Bacillus sp. DUCC-BR-K1.3, Bacillus sp. DUCC-BR-KI.2b, P. flourescens, A. chroococcum, A. vinelandii, Azospirillum  sp., and  A. brazilensis in mix  culture  on peat soil  as carrier. This research done in Microbiology Laboratory of Biology Department Diponegoro University. Subculture and activated culture in Nutrient  Broth medium, make the growth curve to the biomass production, make the inoculums, prepare the peat soil, biomass production and mixed biomass with  the carrier, enumeration bacterial culture viability  test in carrier during the storage  by TPC method.  The  results  show  that  all  consortia  culture  bacteria viabiliry Bacillus  sp. DUCC-BR-KI.3  with Azospirillum (A) and Bacillus  sp. DUCC-BR-K1.7, with P.  flourescens (B)  still viable and increasing number of population during seven weeks storage with  l0 t-n CFU/g at To and up to  l0 r3-ro CFU/g at  the end storage. The  consortium  C  between Bacillus  sp.  DUCC-BR-KI.2  b  and A-  brssilensis  increasing  the  number  of population  and  still viable during eight weeks  storage  (1013  - 1014  CFU/g).Key words: biofertilizer, peat soil, viability,  mix cultare, consortiumPermalink : http://ejournal.undip.ac.id/index.php/sm/article/view/3138

KajianKehilangan Hasil Pada Pengeringan dan Penggilingan Padi di Lahan Pasang Surut Sumatera Selatan

Jurnal Lahan Suboptimal Vol 1, No 1 (2012)
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Raharjo et al., 2000. Study of Yield Losses during Drying and Milling of Paddy in Tidal Lowland of South Sumatra. J. Lahan Suboptimal 1(1):72-82.In tidal lowland of South Sumatra low quality rice called beras batik is commonly found. Beras batik is rice that is low in both quality and prices. The low quality rice grains have high fracture, the percentage of head rice is only 25%, while the percentage of yellow grain exceeds 50%, and its selling price is only Rp. 1800/kg in 2004. The main causes were poor harvest and post harvest handling, delaying harvest and post harvest due to the lack of manpower and facilities owned by farmers. The condition was worsened by the heavy rainfall during harvest time and sometimes coincided with high tide. The study was conducted on July 2011 (dry season) in tidal lowland reclamation area of Telang Rejo village, Muara Telang Sub-district, Banyuasin Regency. In this study 2 post-harvest technologies were introduced to improve harvesting techniques and post harvest handling; (1) Drying assessment of Grain Dryer Machine Fueled withhusk, and (2) Study the Different Configurations of Rice Milling Unit. Lost during drying was 2.77 for sun drying compared to 7.13% with box dryer. Field testing at RMU 1 showed that milling recovery using box dryer (63.5%) was higher than sun drying (61.6%). But, at RMU 2 milling recovery for box dryer (61.3%) was lower than sun drying (63.7%). For milling losses, box dryer (4.99%) was lower than sun drying (5.99%).

Biodegradasi Senyawa Hidrokarbon Oleh Strain Bacillus cereus(VIC) Pada Kondisi Salinitas Yang Berbeda

Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Bacillus cereus has been noted as hydrocarbonoclastic microbe that has ability to degrade hydrocarbons in non-saline conditions and some often to be found on high salinity environtment conditions. The purpose of this research is to determine the ability of strains Bacillus cereus (VIC) were isolated from non-saline environment to degrade hydrocarbons in crude oil on the different salinity condition. Bacillus cereus (VIC) was inoculated on the medium that has contaminated by crude oil with salinity level of 0,3 , 9,4 , dan 19,6 and incubated for 15 days. Determination of microbial growth is by using Total Plate Count (TPC) method along with determination of Total Petroleum Hydrocarbon (TPH) percentage using gravimetry method for every 5 day. The determination of microbial growth showed that Bacillus cereus (VIC) has the tolerancefor salinity level up to 19,6 because it is able to grow to a density of 6.9 x 106 CFU/ml on the 15th day. Bacillus cereus (VIC) is also able to degrades hydrocarbons on crude oil pollutants that indicated from degradation of TPH percentage in the medium up to 21% during 15 days incubation period on the medium with salinity level of 19,6 . Biodegradation using Bacillus cereus (VIC) can increase TPH degradation on the medium up to 19,8% than TPH degradation because of weathering. Keywords: Biodegradation, Bacillus cereus(VIC), Total Petroleum Hydrocarbon (TPH), Salinity.

APLIKASI WEB GRABBER UNTUK MENGAMBIL HALAMAN WEB SESUAI DENGAN KEYWORD YANG DIINPUTKAN

Jurnal Informatika Vol 7, No 1 (2006): MAY 2006
Publisher : Institute of Research and Community Outreach - Petra Christian University

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At this time the use of search engine on the Internet still does not reach maximum result. Users need to browse every web page list as the result of the search that fit with the topic one by one. We need an application that has a capability to look and to save web pages with their links fit with the topic without browsing, so that the result can be accessed offline. This application is called Web Grabber. Web Grabber application uses inputs such as keyword and searching depth. The input will be processed to obtain the appropriate web pages with the keyword and the searching depth, which is automatically saved. Firstly a keyword is needed to be search in the available search engine to get the search lists, which can be picked by the user. Every pick list will be used as the first search page. This application results are web pages that contain the keyword. This application also has a facility to browse the results. This application was succeed to automatically save web pages that appropriate with the input keyword and the depth, that previously search in AltaVista or Google. Web pages that contain with the keyword saved in the computer so that it can be browsed offline. Abstract in Bahasa Indonesia : Saat ini penggunaan search engine pada internet masih belum memperoleh hasil yang maksimal. Pengguna masih harus browsing halaman-halaman web hasil pencarian untuk mencari topik yang diinginkan satu-persatu. Untuk itu perlu dibuatkan aplikasi yang dapat mencari dan menyimpan halaman-halaman web dengan link-link-nya sesuai topik tanpa harus melalui proses browsing, sehingga hasilnya dapat dilihat secara offline. Aplikasi ini biasa disebut Web Grabber. Program aplikasi Web Grabber ini mendapatkan inputan berupa keyword dan kedalaman pencarian. Inputan ini akan diproses untuk memperoleh hasil berupa halaman - halaman web yang disimpan secara otomatis sesuai dengan keyword yang diberikan dan tingkat kedalaman pencariannya. Proses didahului dengan pencarian keyword pada search engine yang tersedia menjadi sebuah list yang dapat dipilih oleh pengguna. Hasil aplikasi ini berupa halaman - halaman web yang mengandung keyword. Aplikasi juga menyediakan fasilitas untuk browsing hasil yang didapat. Program yang dibuat berhasil melakukan penyimpanan halaman-halaman web sesuai dengan keyword yang diberikan dengan tingkat kedalaman tertentu, yang sebelumnya dicari melalui search engine Altavista atau Google. Halaman-halaman web yang mengandung keyword dapat diambil dan disimpan pada komputer sehingga dapat di-browse secara offline. Kata kunci: web grabber, keyword, kedalaman, search engine, internet.

IDENTIFIKASI POTENSI INTERAKSI OBAT PADA PASIEN RAWAT INAP PENYAKIT DALAM DI RSUD Prof. Dr. MARGONO SOEKARJO PURWOKERTO DENGAN METODE OBSERVASIONAL RETROSPEKTIF PERIODE NOVEMBER 2009 - JANUARI 2010

PHARMACIANA Vol 2, No 2: November 2012
Publisher : PHARMACIANA

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Potensi interaksi obat adalah potensi aksi suatu obat diubah atau dipengaruhi oleh obat lain yang diberikan bersamaan. Interaksi obat didefinisikan sebagai fenomena yang terjadi ketika efek farmakodinamik dan farmakokinetik dari suatu obat berubah karena adanya pemberian obat yang lain. Interaksi obat dapat menyebabkan advers drug reaction apabila potensi terjadinya interaksi tersebut tidak diketahui sebelumnya upaya optimalisasi tidak dapat dilakukan. Tujuan penelitian ini adalah mengidentifikasi potensi interaksi obat pada pasien rawat inap penyakit dalam RSUD Prof. Dr. Margono Soekarjo Purwokerto. Metode penelitian adalah observasional retrospektif (November 2009 - Januari 2010) dengan menggunakan metoda deskriptif untuk analisa data. Hasil penelitian menunjukkan potensi interaksi obat pada pasien rawat inap penyakit dalam RSUD Prof. Dr. Margono Soekarjo Purwokerto sebesar 56,76% (n = 259). Berdasarkan kategori signifikansi yang dikemukakan Tatro (2006), terdapat potensi interaksi obat kategori signifikansi 1 pada pasien rawat inap penyakit dalam RSUD Prof. Dr. Margono Soekarjo Purwokerto sebesar 16,60%. Penggunaan obat berpotensi interaksi yang masuk kategori signifikansi 1 sebaiknya disesuaikan dengan kebutuhan pasien akan obat, risk and benefit serta dilakukan upaya optimalisasi.

AKTIVITAS SPESIFIK SELULASE Serratia marcescens DENGAN VARIASI KONSENTRASI AMONIUM SULFAT ((NH4)2SO4) DAN pH

Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Cellulose is a component found in the cellular structure in almost all plant matter, its existence considered to be the most abundant on earth, and even excreted by some bacteria. Cellulose degradation is performed by cellulase enzymes consisting of three components, namely, endoglucanase, exoglucanase, and β-glucosidase enzyme with glucose as the final product. Cellulase utilization is often used in the textile, food and paper industries, whereas in the field of pharmaceuticals, cellulase enzymes are used to maintain optimal digestive health or produce substances that act as binding tablets such as methylcellulose, ethylcellulose, and hydroxypropylcellulose. The purpose of this study is to determine the concentration of ammonium sulfate and optimum pH for cellulose specific activity of Serratia marcescens. Determination of cellulase activity was done by DNS method, while determination of protein content was done by Lowry method. This research uses Randomized Block Design (RAK) factorial pattern with two factors. The first factor was variation of ammonium sulfate concentration which consisted of (0%, 0,75% and 1%). The second factor is the variation of pH consisting of 6, 7, and 8. Each factor is repeated 3 times. The data obtained were analyzed using Analysis Of Variance (ANOVA). The results showed that the combination of ammonium sulphate concentration variation with pH was not optimum to increase cellulose specific activity of S. marcescens.    Keywords: Cellulase, Ammonium sulfate, pH, Serratia marcescens

ISOLASI DAN KARAKTERISASI BAKTERI PENDEGRADASI PESTISIDA DICOFOL DARI TANAH SAWAH DI KABUPATEN KARAWANG

Jurnal Akademika Biologi Vol. 1 No. 1 Oktober 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Karawang was the center of rice production, but rice production in paddy fields in Karawawang often faces obstacle hopper pest that caused crop failure. These condition prompted the farmers to using dicofol pesticides excessively, thus caused pollution of pesticides. The purpose of this research were get the isolates bacteria that could degrade the pesticide dicofol in Karawang paddy fields and discovered characteristics of bacteria that have potential to degrade pesticides. The research was conducted with the isolation and characterisation of bacteria from Karawang paddy soil, the characterisation of isolates bacteria by observation morphological and phenotypic include staining Gram and biochemical tests, test isolates bacteria resistence of dicofol, measuring the growth curve and High Performance Liquid Chromatography (HPLC) for discovering of decreased dicofol concentration during 24, 48, and 72 hours of incubation time. The result is six isolates, that are AA1, AA2, AA3, AA4, AA5, and AA6. Isolates AA1 is suspected as genus Phenylobacterium, isolates AA3 and AA6 are suspected as genus Pseudomonas, AA4 isolates is suspected as genus Bacillus, isolates AA5 is suspected as genus Alcaligenes, but isolates AA2 not yet be identified. The results indicate that isolate AA6 has most resistent to dicofol and most number of cells bacteria, that is 7.9x108. Isolate AA6 is also the most effective in degrading dicofol as much as it can reduce the concentration in amount of 76.0 ppm (84.45%) in MSM medium + 90 ppm dicofol. Keywords : Isolation, Characterisation, Bacteria, Resistention, Degradation, Dicofol Pesticide

PENAPISAN DAN PEMANFAATAN RHIZOBAKTERI TANAMAN SORGUM (Sorghum bicolor L. Moench) SEBAGAI INOKULAN PEMACU TUMBUH TANAMAN

Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Sorghum was a cereal crop that has many benefit such as food, feed, industrial, and bioenergy. Sorghum had a potency to be cultivated, but productivity of sorghum was still low both in quatity and quality. One way to increase production of sorghum  is using rhizobacteria as biofertilizer. The aim of this study is to get rhizobacteria that has the ability to produce IAA, solubility of phospat (P), Nitrogen (N) fixing, and analyze the effect of rhizobacteria inoculants for enhance sorgum plant growth. Isolation of rhizobacteria was done by diluting  rhizobacteria sorghum suspension from 10-1 to 10-5 and it were be platted on SEA medium. Isolates were screened by ability to produce IAA, solubility of P, and N fixing. Producing of IAA test was done by adding Salkowsky reagent on bacterial supernatant and measured absorbance at 530 nm wavelength. Solubility of P test was done by inoculating isolates in Pikovskaya media, while N fixing test was done on N fixing media (NFB). Isolates of rhizobacteria which had a potency to increase growth of plants were made inoculants to be applied in sorghum plants. The result of this study obtain 3 isolates i.e Sr 194.3; Sr 172.1; and Sr 209.1 which were considered effective for increase growth of sorghum. The conclusion  of this study isolates which showed the highest average plant height, root length, and dry weight Sr 194.3 isolate. The statistical analysis among the treatments showed that did not any significant differences on plant height, root length, and dry weight of sorghum age 28 days after farming. Keyword : Increase growth plants, Screening, Shorgum, Rhizophere.