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Journal : Jurnal Biologi

PERILAKU BERTELUR DAN SIKLUS HIDUP Aedes aegypti PADA BERBAGAI MEDIA AIR Agustin, Indira; Tarwotjo, Udi; Rahadian, Rully
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Aedes aegypti is the main vector that transmits dengue fever. The spread of Aedes aegypti mosquitos in Indonesia is very wide. This mosquito has its breeding site in clear water areas such as in the bath water containers, flower pots, pet containers as well as in the used goods that can hold stagnant water. However, the environmental conditions which are constantly changing due to the rampant pollution cause Ae. Aegypti to continue to adapt to its breeding environment. This research aims to find out the egg laying behavior, life cycle and mediums preferences selected by Aedes aegypti mosquitos in different water mediums. Stages of the research which are conducted include: preparation of research tools and materials, observation on Ae. Aegypti mosquitos’ preferences, observation on egg hatchability and life cycle of Ae. Aegypti in various water mediums. The result shows that from all contaminated water mediums which are tested, Ae. Aegypti mosquitos have the preference of laying eggs in Eichhornia crassipes water mediums, followed by leachate water mediums, whereas in tofu wastewater mediums and laundry waste, there were no eggs found. Meanwhile, in another study, it is known that the egg hatchability and life cycle of Ae. Aegypti mosquitos in contaminated water mediums such Eichhornia crassipes immersed water mediums, and leachate water are not significantly different from controlled water mediums (uncontaminated). The result of the research is expected to be the reference for relevant institute in expanding the eradication of Ae. Aegypti mosquitos to places that have been unusual for eradication.Keywords: Aedes aegypti, Eichhornia crassipes immersed water, Leachate, laundry waste, tofu wastewater
UJI EFIKASI BEBERAPA ISOLAT BAKTERI ENTOMOPATOGEN TERHADAP KECOA (Orthoptera) Periplaneta americana (L.) DAN Blatella germanica (L.) DALAM SKALA LABORATORIUM Rini, Monaliza Sekar; Rahadian, Rully; Hadi, mochamad; Zulfiana, Deni
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Cockroaches are the insect vectors of disease that can cause adverse effects on human health. Control cockroaches excessive use of insecticides can lead to residues in the environment and resistance cockroach. Therefore it is necessary to control the use of alternatives such as by biological agents such as entomopathogenic bacteria. This research used two isolates of entomopathogenic bacteria isolated from Spodoptera litura were found dead, Bacillus thuringiensis IPBCC collection and sterile distilled water as a control. Tests conducted by the spray and bait method at a concentration of 108. The results showed that all isolates entomopathogenic bacteria used in this research can cause mortality of cockroaches. Morphological observation and Koch's postulates test showed that the mortality of cockroaches likely caused by bacterial isolates were granted. SP4 bacterial isolates using spray method provides the highest mortality was 26.67% of the P. americana and 80% against B. germanica. Isolates of B. thuringiensis using bait influence the highest mortality of 10% of the P. americana and 6.67% against B. germanica. LT-50 was the most effective method of treatment of bacterial isolates SP4 spray against B. germanica was 2 hours 30 minutes 46 seconds.Keywords: Biological control, entomopathogenic bacterial, efficacy test
IDENTIFIKASI KANDUNGAN SENYAWA FITOKIMIA MINYAK BIJI MIMBA (Azadirachta Indica, A. Juss) Palupi, Dyah; Kusdiyantini, Endang; Rahadian, Rully; Prianto, A Heru
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Neem seeds oil (Azadirachta indica, A. Juss) have phytochemical compounds which act as antifeedant, repellent, antibacterial, and antifungal. The neem seeds oil were important identified their phytochemical compounds. Identifications were done with screening phytochemical and infrared spectrophotometer. Neem seed oils were obatained with pressing method. The group of compounds tested were alkaloid, flavonoid, triterpenoid, steroid, saponin and tannin. The results of phytochemical showed that neem seeds oil consist of alkaloid, flavonoid, triterpenoid, steroid, saponin and tannin. This results were strengthned with spectrum in infrared spectrophotometer that showed the functional groups of each those compounds. Keywords: neem seeds oil, phytochemical compounds, pressing method, functional groups
KEANEKERAGAMAN NGENGAT DI WANA WISATA GONOHARJO, LIMBANGAN, KENDAL, JAWA TENGAH Kamaludin, Nanang; Hadi, Mochamad; Rahadian, Rully
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Moths is insect in Order Lepidoptera of Nocturnal habit. Moths help to polinate the flower were blossom in the night. The relationship of moths and plants can be used as bio-indicators of environmental changes in the Gonoharjo forest habitats. The objective of this study are to compare the abundance and diversity of members of moth (Lepidoptera) in various habitats and to determine the status of rare and endemic species that are found in Wana Wisata Gonoharjo. This study used light traps method for moth in four different habitats, i.e., secondary forests, riparian area and forests of pine forest and coffee plantation. The research was conducted over two months during October-November 2012. The analysis used were relative abundance, diversity index, Huctheson test, evennes index and similarity index. Threety nine moth species from 10 families were found in 4 habitats of Wana Wisata Gonoharjo. The highest number of moth species found in secondary forest (30 species) and the lowest one found in the pine forests (6 species). Statistically, there were differences within the types of habitat diversity. Evennes indices in all habitats of Wana Wisata Gonoharjo were relatively high. In general, type of habitat affects species diversity of moths. Keyword : Diversity, moth and Gonoharjo forest.
PREFERENSI BERTELUR NYAMUK Aedes aegypti L. BERDASARKAN JARAK PENEMPATAN OVITRAP BERMEDIA AIR DOMESTIK TERHADAP OVITRAP BERMEDIA AIR RENDAMAN JERAMI Hidayah, Wahyu Nur; Hidayat, Jafron Wasiq; Rahadian, Rully
Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Ovitrap is one of the environmentally friendly equipment of controlling Aedes aegypti which causes dengue fever. The utilization of ovitraps by adding attractant substances which are placed in certain distances can stimulate the mosquitoes’ sense of smell in order to lure them. The objective of this study were to determine the effective placing distance of ovitrap’s domestic water media on ovitrap’s hay-soaked water medium in order to lure Ae. aegypti on laying eggs, and to understand the oviposition preferences of Ae. aegypti based on the  placing distance of ovitrap’s domestic water media on ovitrap’s hay-soaked water medium. This research design was factorial experiment by using Split Plot. The water medium used was hay-soaked water as the control. The domestic water media used were well water, tap water, and rain water which were placed with the distance of 0 meter, 1 meter, 3 meters, and 5 meters from the hay-soaked water by using 3 replications. The independent variable were the placing distance and water media, while the dependent variable was the number of eggs trapped. The data were analyzed by using two-way ANOVA with the significance level of 95%. The findings showed that the placing of ovitrap’s well water and tap water with the distance of 5 meters is still effective to distract the oviposition preferences of Ae aegypti on hay-soaked water, while in the well water was only effective up to 3 meters. The water media has no effect on oviposition preferences of Ae aegypti when they are placed up to 5 meters away from the hay-soaked water. This happens due to the high concentration of ammonia in the hay-soaked water which attract the mosquitoes to choose compared to other water media to lay eggs.    Keywords: Aedes aegypti, ovitrap, attractant, hay-soaked water, ammonia
Pengaruh Rendaman Eceng Gondok (Eichhornia crassipes [Mart.] Solms) Terhadap Daya Predasi Mesocyclop jakartensis Alekseev Absarina, Dheanda; Rahadian, Rully; Hadi, mochamad
Jurnal Akademika Biologi Vol. 4 No. 1 Januari 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Aedes aegypti has been reported as vectors of diseases, including dengue fever (dengue hemorrhagic fever) and Chikungunya. Various efforts have been done to overcome A. aegypti either physically, chemically and biologically control. However, these efforts have not been successful in reducing the density of the vector. The objectives of this study was to compare predation of M. jakartensis on A. aegypti mosquito larvae in several type of water i.e., well water (control), hyacinth infusion and hay infusion. The research method used complete randomized factorial design. Its effect on predation capability test of M. jakartensis used bioassay method on 25 initial instar larvae and 5 individuals of M. jakartensis. Predation capabilities test was analyzed using one-way ANOVA test. Correlation of the chemical factors on capabilities of M. jakartensis predation on the treatments was analyzed statistically using the Spearman test. The results show that predation capabilities of                      M. jakartensis on A. aegypti larvae are as follows, control > Hyacinth (3: 7) > Hay (1: 9) > Hyacinth (1:9). The percentage of predation for each treatment are 93,3; 72,0; 66,7 and 64,0%, respectively. Statistically, predation capabilities of M. jakartensis on A. aegypti larvae in each treatmens is not significantly different (P > 0.05). Hyacinth infusion is unproven in improving the predation of M. jakartensis on A. aegypti larvae, but it does not inhibit capabilities of M. jakartensis predation.  Keywords : Aedes aegypti, Eichhornia crassipes, Mesocyclop jakartensis
PREFERENSI PELETAKAN TELUR DAN PENGHAMBATAN PERKEMBANGAN PRADEWASA NYAMUK Aedes aegypti L. DI BERBAGAI MEDIA AIR Sabila, Makna Fathana; Rahadian, Rully; Hidayat, Jafron Wasiq
Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Water media influences the effectiveness of the use of ovitrap media for Ae. aegypti. Water media which is prefered by the mosquito as well as inhibition the premature growth have huge potency in controlling Ae. aegypti. The objective of this study were to investigate the preference of oviposition of Ae. Aegypti and to determine the premature growth of Ae. aegypti in water media containing a combination of organic matter and different chlorine concentrations, also to examine the interaction between organic matter and chlorine to the preferences of oviposition and the premature growth of Ae. Aegypti. The method of this study is done by experiment by applying Completely Randomize Design (RAL). This study used two types of water media combination. The media combinations contained organic materials and chlorine with seven different treatments. They were well water (control), water added with soil and hay-soaked water added with chlorine by 5, 10, and 15 ppm respectively. The findings show that Ae. aegypti have the tendency in choosing hay-soaked water in lay the eggs since it has abundant content of organic matter in it. The mean amount of Ae. aegypti oviposition in the water medium combination ranged from 84 to 152.7 eggs, while the percentage of premature survival rate ranged from 59.4% to 97%. In conclusion, the study showed that the preference of oviposition is highly influenced by the organic matter content of the media. Both organic matter and chlorine do not inhibit the premature growth of Ae. aegypti. However, the interaction between organic matter and chlorine to the preferences of oviposition and the premature growth of Ae. aegypti was not found. Keywords: Aedes aegypti, combination, ovitrap, organic matter, chlorine.
STRUKTUR KOMUNITAS LARVA TRICHOPTERA DI SUNGAI GARANG SEMARANG Purdyaningrum, Lila Ris; Rahadian, Rully; Muhammad, Fuad
Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

River is the natural habitat for macrobiotic organisms, one of them is Trichoptera larvae.      Trichoptera larvae can be used as bio-indicator of water pollution because it is sensitive to environmental and habitat characteristics changes. The objective of this research is to examine the community structure of Trichoptera larvae in Garang River Semarang and the river quality based on the biological and physical-chemical factors. This study used survey method and purposive sampling technique to collect the sample.      The locations of the research are four stations which were determined by the land use around the river. Three samples were taken from each station by using 25 x 40 cm surber net. The findings show that the Trichoptera larvae found in four Garang River Semarang observation stations consisted of five genus; they are Cheumatopsyche, Chimarra, Glossosoma, Hydropsyche, and Tinodes. Relative abundance of Glossosoma and Cheumatopsyche at station I were almost balance, thus there was no dominant genus in the station. Trichoptera larvae dominated station II are Glossosoma and Hydropsyche. The Cheumatopsyche and Chimarra were the dominant genus in station III. While at the station IV, the researcher only found Hydropsyche. Trichoptera larvae which were highly diverse was in station III (H' = 1.41) and the lowest was in station IV    (H' = 0). Trichoptera larvae spread evenly in station I, station II, and station III, while station IV was dominated by genus Hydropsyche. In conclusion, the study showed that the highest density, abundance, and diversity level of the Trichoptera larvae in Garang River was in Tinjomoyo area, which had substrate rocks and fast water currents. Based on biological and physical-chemical factors, the condition of the four observation stations                        in Garang River could be grouped into two categories; not polluted and polluted. Keyword: Community structure, Trichoptera larvae, Garang River
STRUKTUR KOMUNITAS BRYOFAUNA LUMUT EPIFIT PADA ZONA MONTANA DI KAWASAN GUNUNG UNGARAN, SEMARANG, JAWA TENGAH Anggraini, Lucia Puspita; Rahadian, Rully; Hidayat, Jafron Wasiq
Jurnal Akademika Biologi Vol. 3 No. 1 Januari 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Plant moss (bryoflora) is in important in the tropical forest, including Ungaran mount which is significantly play an  important role in water balance. Bryofauna can be found in moss attacking on the tree and is often referred  as moss epiphytic bryofauna. The objective of the research  were to compare the abundance and diversity of moss epiphytic bryofauna in Montana zone at three different altitudes of Ungaran mount. The sampling methods used was square plots. Bryophytes were collected in 10x10 cm plots on tree trunks. The specimens were extracted using Barlese funnel up to seven days at Laboratory of Ecology and Biosistematics, Departement of Biology, Diponegoro University. The results shows there were 5 classes of 15 ordes and 27 sub ordes/families. The highest abundance of moss epiphytic bryofauna was  in altitudes 2040 m asl (24.175 individu/m2), while the lowest was in altitude 1355 m asl (10.275 individu/m2). The biggest diversity of moss epiphytic bryofauna was found in altitude 2040 m asl (H’=2,20), while the lowest was in altitude 1355 m asl (H’=2,00). Acarina has the highest abundance in a these three altitudes. Keywords : Community Structure, Epiphytic Bryophytes, Bryofauna, Montana Zone
STRUKTUR KOMUNITAS MIKROARTHROPODA BRYOFAUNA EPIFIT DI ZONA TROPIK GUNUNG UNGARAN, JAWA TENGAH Yuniarti, Rina Eka; Rahadian, Rully; Perwati, Lilih Khotim
Jurnal Akademika Biologi Vol. 2 No. 1 Januari 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

       Mount Ungaran has various diversity of flora, including bryophytes. Epiphytic bryophytes are the habitat of bryofauna most of them are  microarthropods. The objective of this research was to determine community structure of epiphytic bryofauna in tropical zone of Mount Ungaran. This research was conducted in tropical zone of Mount Ungaran at three different  altitudes. The sampling methods was using square plots. Bryophytes were collected in plots 10x10 cm on tree trunks. Furthermore, the specimens were extracted using Barlese funnel at Laboratory of Ecology and Biosistematics, Department of Biology, Diponegoro University up to seven days. The results shows there are 5 classes of 16 orders and 30 sub orders/families. The most diverse of epiphytic bryofauna found at altitude 980 m asl and 1100 m asl (H’= 2,30), while the lowest at 750 m asl (H’= 1,87). Order of Acarina is consistantly found dominant in all altitudes. Meanwhile, at 1100 m asl the order of Thysanopera has also high abundant or dominant. Keywords : community structure, epiphytic bryophytes, bryofauna, tropic zone.