Articles

Antibacterial Property of a Coral-Associated Bacterium Pseudoalteromonas luteoviolacea Against Shrimp Pathogenic Vibrio harveyi (In Vitro Study) RADJASA, OCKY KARNA; MARTENS, TORBEN; GROSSART, HANS- PETER; SABDONO, AGUS; SIMON, MEINHARD; BACHTIAR, TONNY
HAYATI Journal of Biosciences Vol 12, No 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

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Abstract

A coral-associated bacterium was successfully screened for secondary metabolites production based on PCR amplification of the nonribosomal peptide synthetase gene and was identified as closely related to Pseudoalteromonas luteoviolacea based on its 16S rDNA.The bacterium was found to inhibit the growth of shrimp pathogenic bacterium tested, Vibrio harveyi. To characterize the inhibiting metabolite, a 279 bp long DNA fragment was obtained and the deduced amino acid sequence showed conserved signature regions for peptide synthetases and revealed a high similarity to NosD (40% identity), a multifunctional peptide synthetase from Nostoc sp. GSV224, and NdaB (44% identity), a peptide synthetase module of Nodularia spumigena.
APPLICATION OF REPETITIVE SEQUENCE-BASED PCR ON THE RICHNESS OF VIBRIO ON THE TIGER SHRIMP (Penaeus monodon Fab.) arjito, S; Ningrum, Nestin E.W.; Radjasa, Ocky Karna; Prayitno, Slamet Budi
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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A molecular-based study was conducted to estimate the richness of the Vibrio on ?tiger shrimp (Penaeus monodon Fab.) from brackish water pond of Mororejo, Kendal. Tiger shrimps were collected from the extensive brackish water pond and a total of 22 isolates were obtained from hepatopancreas and telson of tiger shrimp. Based on the repetitive sequence-based polymerase chain reaction (rep-PCR), it was found that two groups of Vibrio. To investige the efectiveness of rep-PCR in estimating the richness of Vibrio on tiger shrimps, three isolates ?(JTW 01, JTW 03 and JTW 06) were chosen for further investigation. On the basis of sequence analysis, the result showed ?that the JTW 01, JTW 03 and JTW 06 were closely related to Vibrio sp. Absa7 clone 423.1, Vibrio splendidus and Vibrio splendidus, respectively. ?The result proved that two assosiated of Vibrio on tiger shrimp were Vibrio sp. Absa7 clone 423.1 and Vibrio splendidus. Therefore ?the present study highlights the effectiveness of rep-PCR in rapid grouping and estimating the richness of Vibrio on ?tiger shrimp.
Karakterisasi Molekuler Bakteri yang Berasosiasi dengan Penyakit BBD (Black Band Disease) pada Karang Acropora sp di Perairan Karimunjawa Sabdono, Agus; Radjasa, Ocky Karna
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 11, No 3 (2006): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Black Band Disease (BBD) merupakan penyakit yang bersifat virulen terutama menyerang jenis karang batu.Meskipun komunitas bakteri BBD didominasi oleh jenis cyanobakterium, namun penelitian tentang jumlahkomposisi bakteri yang menyusun komunitas belum pernah dilakukan. Komunitas bakteri yang berasosiasi dengan penyakit BBD (black band disease) pada karang cabang Acropora sp. dalam penelitian ini diujidengan menggunakan teknik kultur dependent. Teknik molekuler gen 16S rDNA (amplifikasi 16S DNA ribosom) digunakan untuk karakterisasi komunitas secara komprehensif. Berdasarkan analisis sekuen gen 16SrDNA, data menunjukkan bahwa isolate BBD1 memiliki kekerabatan terdekat dengan Myroides odoratimimus (99.0%), isolat BBD2 adalah Bacillus algicola (99.6%) dan isolat BBD3 adalah Marine Alcaligenaceae bacteriumico (96.0%). Hasil identifikasi bakteri yang berasosiasi dengan penyakit BBD pada karang cabang Acropora sp di Karimunjawa merupakan komunitas baru yang berbeda dengan hasil penelitian terdahulu. Hasil inimemungkinkan untuk dilakukan penelitian lanjutan tentang isolasi dan kultur bakteri tersebut untuk bisa menerangkan etiologi penyakit.Kata kunci: Black Band Disease (BBD), Acropora sp., 16S rDNA.Black band disease (BBD) is a virulent disease primarily affecting scleractinian corals. Eventhough the BBD bacterial mat is dominated by a cyanobacterium, the quantitative composition of the BBD bacterial matcommunity has not described previously. The bacterial community associated with black band disease (BBD) of the branching corals Acropora sp. in this study was examined using culture-dependent techniques. Acomplementary molecular techniques of 16S rDNA genes [amplified 16S ribosomal DNA) was used to give a comprehensive characterization of the community. On the basis of the results of sequen analysis, our datashow that BBD1 isolate was closely related with Myroides odoratimimus (99.0%), BBD2 isolate was Bacillus algicola (99.6%) and BBD3 isolate was Marine Alcaligenaceae bacterium (96.0%). Of the three bacteria identified,these were not previously found in other studies. This result will allow the dominant BBD bacteria to be targeted for isolation and culturing experiments designed at interpreting the disease etiology.Key words: Black Band Disease, Acropora sp., 16S rDNA.
Kajian Temporal Indeks Pencemaran Akibat Pengaruh Limbah Domestik dan Pola Penyebarannya di Perairan Pantai Kartini, Jepara Bachtiar, Tonny; Khasanah, Uswatun; Radjasa, Ocky Karna; Suhartono, Edi; Hardiyanto, Agus
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 10, No 4 (2005): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Penelitian ini dilakukan pada kondisi musim kemarau (Agustus 2003, monsun Timur), dan pada kondisi musim hujan (Maret 2004, kondisi monsun Barat), pada tiga kondisi lingkungan, yaitu: sungai dan muara Sungai Demaan, dan perairan pantai Kartini Jepara. Pengukuran beberapa parameter kualitas air dan pengambilan sampel air dilakukan pada 12 stasion. Dengan menggunakan metoda Indeks Pencemaran (pollution indexs) diketahui bahwa indeks pencemaran pada kondisi musim kemarau mempunyai nilai yang lebih tinggi dari pada kondisi musim hujan, baik di lingkungan sungai, muara, naupun perairan pantai. Kondisi lingkungan sungai pada kondisi musim kemarau mempunyai kriteria pencemaran cemar berat, dengan IP 11,48, pada kondisi musim hujan menjadi cemar ringan, dengan IP 4,49. Pada kondisi lingkungan muara saat musim kemarau, termasuk cemar sedang dengan IP 6,71, sedangkan pada kondisi musim hujan menjadi cemar ringan, dengan IP menjadi 3,11. Status mutu air di perairan pantai ke arah laut hingga stasiun kontrol termasuk dalam cemar ringan, baik pada kondisi musim kemarau maupun kondisi musim hujan. Secara umum pola penyebaran Indeks Pencemaran pada monsun Timur dan monsun Barat, mempunyai pola yang relatif tinggi di sekitar muara dan menurun dengan meningkatnya jarak dari muara, dan tersebar mengikuti dinamikaperairan ke Selatan, kemudian ke Baratdaya mengikuti bentuk pantai Teluk Kartini.Kata kunci: indeks pencemaran, monsun, muara, perairan pantai, sungaiThis research was conducted on the condition of dry season (August 2003, East monsoon), and the condition of wet season (March 2004, West monsoon), on three environmental conditions: river and river mouth ofDemaan River, and Kartini coastal waters, Jepara. Measurement of several water quality parameters and collecting of water samples was carried out at 12 stations. By using Pollution Index method, it could be recognized that pollution indexes (PI) in the river, river mouth, and coastal waters during dry season were higher than during wet season. In the river environment during dry season was heavy polluted with PI 11.48,and during wet season became light polluted with PI 4.49. River mouth environment during dry season was fairly polluted with PI 6.71, and during wet season became light polluted with PI 3.11. The status waterquality of coastal water to the sea direction until control station was in light polluted, both in dry and wet seasons. In general, the pattern distribution of PI in East and West monsoon were relatively higher around theriver mouth and decreased with increasing of distance from the river mouth, and distributed by dynamic of waters to the South, and then to the Southwest went along the coast of Kartini Bay.Key words: pollution index, monsoon, river mouth, coastal waters, river.
Causitive Agent Vibriosis dari Ikan Kerapu Bebek (Cromileptis altivelis) Bermulut Merah : 1. Patogenitas pada Ikan Kerapu Macan (Epinephelus fuscoguttatus) Sarjito, Sarjito; Radjasa, Ocky Karna; Hutabarat, Sahala; Prayitno, Slamet B
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 3 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

The research aims were to find out the causative agent vibriosis of Cromileptis altivelis ?having clinical symptom red mouth and its pathogenecity to Epinephelus fuscoguttatus.?? Seven isolats Vibrio were isolated from wound and kidney of ?C.? Altivelis. ?The result of Koch postulate indicated that five vibrios as a causative agent of vibriosis, consisted of three vibrios (isolat JT 07,?? JT 10, JT 20) and ?two vibrios (isolat JT 4,? JT 29) caused? mortality of 100% and 40% on E. fuscogutatus respectively.? Three isolat vibrios ( JT 7,?? JT 10, JT 20 ) with higher pathogenicity were continued to futher investigation. ?Mean time to death of? V. fuscus (JT 07), V. alginolyticus and V. anguillarum, to E. fuscogutatus? on concentration of 108 CFU/mL ?were 83,33%? (11,25 hours); 79,16%(15,63 hours); dan 50% (20,5 hours) respectively; whereas ?on concentration of 109 CFU/mL were 95,83% (10,8 hours); 87,5%(15,28 hours); dan 62,5% (19,6 hours) respectively. Lethal Concentration Median (LC50) of V. Fuscus, V. alginolyticus, ?V. anguillarum were? 3,2X107 CFU/mL; 4,8 X 108 CFU/mL; dan 2,24X108 CFU/mL. All isolates on concentration of 106 dan 107 CFU/mL did not cause 50% ?tested fish mortality Key words : Causative agent, Vibriosis, E. Fuscogutatus, ?V. parahaemolyticus, Pathogenicity?Penelitian ini bertujuan untuk mengkaji causative agent ?vibriosis pada ikan Kerapu Bebek (Cromileptis altivelis) dengan gejala klinis mulut merah serta patogenisitasnya terhadap ikan Kerapu Macan (Epinephelus fuscoguttatus).? Tujuh ?isolat Vibrio berhasil diisolasi dari bagian luka dan ginjal Kerapu Bebek Mulut Merah.? Hasil uji postulat koch memperlihatkan lima isolat dimana isolat ?JT 07,?? JT 10, JT 20 dapat mengakibatkan kematian 100%, sedangkan isolat? JT 04 dan JT 29 menyebabkan kematian 40%. Pada tiga isolat (Vibrio JT 07, JT 10 dan JT 20) yang memperlihatkan patogenitas yang lebih tinggi dilakukan uji lanjutan. ?Hasil karakterisasi melalui uji morfologi dan biokimia diperoleh bahwa isolat JT 07 memiliki kemiripan 96,15 % dengan Vibrio fuscus; ?JT 10 memiliki kemiripan 100% dengan Vibrio anguillarum dan JT 20 memiliki kemiripan 100% dengan Vibrio alginolyticus. Patogenisitas ketiga isolat vibrio tersebut secara berurutan adalah V. fuscus, V. alginolyticus, ?V. anguillarum, dimana diperoleh bahwa? persentase kematian dan rerata waktu kematian (Mean Time to Death, MTD) pada penyuntikan intraperitoneal V. fuscus (JT 07), V. alginolyticus (JT 20) dan V. anguillarum (JT 10) dengan konsentrasi 108 CFU/mL adalah berturut-turut 83,33%? (11,25 jam); 79,16% (15,63 jam); dan 50% (20,5 jam); sedangkan untuk konsentrasi 109 CFU/mL secara berurutan adalah 95,83% (10,8 jam); 87,5% (15,28 jam); dan 62,5% (19,6 jam). Lethal Concentration Median (LC50) V. fuscus,? V. ?alginolyticus, ?V. ?anguillarum secara berurutan adalah ?sebesar? 3,2X107 CFU/mL; 4,8 X 108 CFU/mL; dan 2,24X108 CFU/mL.? Sedang pada konsentrasi 106 dan 107 CFU/mL semua isolat tidak menimbulkan kematian pada ikan uji.? Hasil ini menunjukkan bahwa tiga causative agent tersebut bersifat patogen pada ikan kerapu. Kata kunci:? Causative agent, Vibriosis, E. fuscogutatus V. parahaemolyticus, Patogenisitas
Isolasi dan Identifikasi Bakteri Pendegradasi Koprostanol dari Lingkungan Sungai, Muara, dan Perairan Pantai Banjir Kanal Timur Semarang pada Monsun Timur Munir, Misbakul; Afiati, Norma; Radjasa, Ocky Karna; Sabdono, Agus; Bachtiar, Tonny
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 2 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Aktifitas manusia yang terus meningkat di wilayah pesisir, telah menyebabkan terjadinya peningkatan tekanan lingkungan yang berasal dari berbagai macam sumber pencemaran, antara lain limbah industri, pertanian, transportasi, dan limbah domestik. Salah satu sumber pencemar yang cukup dominan di lingkungan perairan pantai adalah pencemaran akibat limbah domestik. Selama ini yang dipakai untuk mengetahui pencemaran lingkungan oleh limbah domestik adalah menggunakan indikator biologi yaitu bakteri coliform. Namunpenggunaan bakteri coliform sebagai indikator pencemaran limbah domestik mempunyai permasalahan antara lain tidak terdeteksinya bakteri coliform tersebut pada perairan pantai, sementara diduga kuat bahwa perairan tersebut tercemar oleh limbah domestik termasuk feces. Oleh karena itu indikator alternatif sangat diperlukan. Salah satu indikator alternatif pencemaran limbah domestik adalah koprostanol, yang mempunyai sifat cukup konservatif, dapat dikuntitatifkan dan dapat dihubungkan dengan sumber pencemar yang spesifik. Namun perlu diingat bahwa di alam, koprostanol mengalami proses degradasi oleh bakteri, sehingga perlu dilakukan penelitian mengenai isolasi dan identifikasi bakteri pendegradasi koprostanol pada lingkungan sungai, muara, dan perairan pantai Banjir Kanal Timur Semarang pada monsun timur. Penelitian dilakukan pada bulan Juli s/d Agustus 2003 pada lingkungan sungai, muara, dan perairan pantai Banjir Kanal TimurSemarang. Isolasi bakteri dari sampel air dan sedimen dilakukan di Laboratorium Mikrobiogenetika, FMIPA Jurusan Biologi UNDIP Semarang dan identifikasi bakteri pendegradasi koprostanol terseleksi dilakukan diLaboratorium Hama dan Penyakit Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara. Hasil penelitian diperoleh bahwa bakteri pendegradasi koprostanol yang berasal dari lokasi Banjir Kanal TimurSemarang pada berbagai variasi lingkungan dan habitat diperoleh 69 isolat (83,13%) dari 83 isolat yang diuji. Sedangkan hasil identifikasi terhadap 6 isolat terbaik diperoleh 3 (tiga) genus bakteri mampumendegradasi koprostanol, yaitu Achromobacter, Bacillus, dan Branhamella. Dari 3 genus bakteri pendegradasi koprostanol yang didapatkan, ada 2 (dua) genus yang selama ini belum dilaporkan, yaitu genus Achromobacter, dan Branhamella.Kata kunci : Koprostanol, Limbah Domestik, Indikator Pencemar, Isolasi, Identifikasi, BakteriIncreasing human activities in coastal areas have caused an increase of environmental pressures that come from various sources such as industrial disposal, agriculture, transportation, and domestic wastes. One of dominant sources in coastal waters is contamination by domestic wastes. So far to determine environmental contamination by domestic waste is by using biological indicator, coliform bacteria. However the use ofcoliform bacteria have problems for example, they cannot be detected in coastal waters contaminated by domestic waste including faeces. Therefore, an indicator alternative is urgently needed. Alternative indicatorof domestic waste contamination is coprostanol, which is conservative, easy to quantity and related to specific pollutant source. It is important to note coprostanol is naturally degraded by indigenous bacteria.Therefore it is necessary to conduct a study on isolation and identification of coprostanol-degrading bacteria in river, estuarine, and coastal environments of Banjir Kanal Timur Semarang during dry season. The research had been carried out from July to August 2003 at environmental of river, estuarine, and coastal of Banjir Kanal Timur Semarang. Isolation of bacteria from water and sediment samples were conducted atMicrobiogenetics Laboratory, Faculty of Mathematics and Natural Sciences Diponegoro University, meanwhile identificaton of coprostanol-degrading bacteria selected was conducted by at Pest and Diseases Laboratory,Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara. The result showed that coprostanol-degrading bacteria obtained from Banjir Kanal Timur Semarang at various environments and habitat were 69 bacterial isolates (83,13%) from 83 isolat tested. Identification revealed that (three) genus were found to degrade coprostanol, namely Achromobacter, Bacillus, and Branhamella. From 3 genus of coprostanol-degrading bacteria identified, 2 of them : Achromobacter, and Branhamella have not been reported so far.Key words : Coprostanol, Domestic Waste, Pollution Indicator, Isolation, Identification, Bacteria
Kandungan Koprostanol dan Bakteri Coliform Pada Lingkungan Perairan Sungai, Muara, dan Pantai di Banjir Kanal Timur, Semarang Pada Monsun Timur Atmojo, Tri Yuni; Bachtiar, Tony; Radjasa, Ocky Karna; Sabdono, Agus
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 1 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Limbah domestik merupakan salah satu sumber utama pencemaran di perairan pantai pada negara yang sedang berkembang yang masih kurang mendapatkan perhatian serius bila dibandingkan dengan pencemaran oleh industri. Namun dengan terus meningkatnya aktivitas manusia di wilayah pesisir dan kesadaran akan pentingnya lingkungan bersih bagi kesehatan, estetika dan alasan ekologis lainnya, deteksi tentang kontaminasi limbah menjadi penting untuk diketahui secara lebih baik. Selama ini indikator kontaminasi limbah domestik ditentukan berdasarkan jumlah mikroorganisme intestinal khususnya kelompok bakteri coliform. Koprostanol diusulkan sebagai alternatif indikator limbah domestik, sehingga diperlukan kajian eksistensi koprostanol untuk persyaratan kelayakannya sebagai indikator, serta bakteri coliform sebagai pembanding. Penelitian dilakukan pada bulan Juli-Agustus 2003 pada lingkungan sungai, muara dan pantai di sungai Banjir Kanal Timur di Semarang. Analisa coliform dari sampel air dan sedimen dilakukan di laboratorium Mikrobiogenetika, FMIPA UNDIP Semarang dan analisa konsentrasi koprostanol dilakukan di laboratorium Kimia dan FisikaPusat (LAKFIP) UGM Yogyakarta. Hasil menunjukkan bahwa koprostanol dapat terdeteksi pada sedimen dan tidak terdeteksi pada kolom air. Eksistensi koprostanol didapatkan nilai tertinggi pada lingkungan perairansungai (14,9 ?g/g) dibandingkan muara (1,04 ?g/g), dan pantai (5,25 ?g/g). Bakteri total coliform terdeteksi pada kolom air maupun sedimen pada lingkungan perairan sungai (2,80 x 104 sel/100 ml), muara (0,4 x104 sel/100 ml), dan pantai (0 - 0,4 x 104) sel/100 ml, sementara fecal coliform terdeteksi di lingkungan perairan sungai (2 x 104 - 2,8 x 104 ) sel/100 ml, dan muara (0,4 x 104 sel/100 ml), namun tidak terdeteksipada lingkungan perairan pantai.Kata kunci : Koprostanol, limbah domestik, indikator pencemaran, coliformDomestic waste is one of the major sources of the pollution in coastal waters of most developing, countries, which has got less attention than industrial pollution. However, along with the increase of human activitiesin coastal areas coupled with the importance of clean environment for the health, esthetics, and ecological reasons, the detection of waste contamination has become important to be recognized. So far, the indicator of domestic waste contamination has been intestinal microorganism, especially coliform bacteria. Coprostanol is a proposed alternative indicator in the detected domestic waste those, it is definitely important to study the existence of coprostanol and coliform bacteria in order to fine its application. The research carried out from July to August 2003 at the environmental waters of river, estuarine, and coastal of Ciliwung, Jakarta; Banjir Kanal Timur, Semarang, analysis of coliform form water and sediment samples was conducted at micro biogenetic laboratory Faculty of Mathematic and Natural sciences, Diponegoro University, meanwhile analysis of coprostanil concentration was performed at central laboratory of chemistry and physic UGM. The results showed that coprostanol was detected in sediment but not in the water phase at all locations. The existence of coprostanol was detected either at environmental waters of river (14,9 ?g/g) or estuarine (1,04 ?g/g), and coastal (5,25 ?g/g). Total coliform bacteria were detected both in water column and sediment of river (2,80 x 104 ) sel/100 ml, at estuarine (0,4 x 104) sel/100 ml and coastal (0 - 0,4 x104) sel/100 ml, meanwhile fecal coliform bacteria were detected in environmental water of river (2 x 104 - 2,8 x 104 ) sel/ 100 ml, and at estuarine (0 - 4 x 104) sel/100 ml, but not detected at coastal area. The use of coprostanol to considered for the indicator alternative because the existence can influence by various condition at water of river and used of coliform bacteria as domestic waste contamination indicator require to be considered because owning various weakness.Key words : Coprostanol, coliform, domestic waste, pollution indicator
PHYLOGENETIC CHARACTERIZATION OF PSYCHROPHILIC ° BACTERIA ISOLATED FROM DEEP-SEA WATER OF THE NANKAI TROUGH Radjasa, Ocky Karna; Kita-Tsukamotoz, Kumiko; Urakawal, Hidetoshi; Ohwadai, Kouichi
JOURNAL OF COASTAL DEVELOPMENT Vol 4, No 3 (2001): Volume 4, Number 3, Year 2001
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

A screening among 4"C-culturable isolates obtainedfom dcep~sea water of Nankai Trough from a depth of approximately 4,000 m resulted in a total of 5 psychrophilic bacteria showing no growth at 20? C. Phylogenetic characterization based on l6S ribosomal DNA sequence analysis showed that the isolates belonged to the genus Colwellia, Moritella, and CNPT-3, which could be assigned to Psychromonas.
DEEP- SEA BACTERIA AND THEIR BIOTECHNOLOGICAL POTENTIALS radjasa, Ocky Karna
JOURNAL OF COASTAL DEVELOPMENT Vol 7, No 3 (2004): Volume 7, Number 3, Year 2004
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Deep-sea environment characterized by its low temperature, high hydrostatic pressure, limited nutrient contents, and constant darkness has been a hostile environment for most marine microorganisms. Therefore it is a home for low temperature and high pressure-loving bacteria believed to functionally over shallow water intruders. Deep-sea has been regarded as rainforests in terms of their microbial diversity. Thus, the deep-sea should commend itself to microbiologists and biotechnologists alike as source of novel microorganisms and exploitable properties. Near Indonesia several deep-sea trenches exist with diverse environmental conditions. However, up to now there has been no effort to study the microbial communities of those deep-sea environments by Indonesian scientists. This is mainly because of the lack of knowledge in the field of deep-sea microbiology in Indonesia.
ANTIFOULING ACTIVITY OF BACTERIA ASSOCIATED WITH SOFT CORAL Sarcophyton sp. AGAINST MARINE BIOFILM-FORMING BACTERIA Sabdono, Agus; Radjasa, Ocky Karna
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 1 (2006): Volume 10, Number 1, Year 2006
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Marine bacteria associated with soft coral Sarcophyton sp collected from vicinity of Peucang island, Ujung Kulon, West Java, were successfully screened for antifouling activity against marine biofilm-forming bacteria isolated from the surrounding colonies of Sarcophyton sp. Six bacterial isolates were found to inhibit the growth of at least one of 7 biofilm-forming isolates.? The most active strain USP3.37 was identified as Pelagiobacter variabilis by using 16S rDNA gene sequence analysis. Similarly, the active strains USP3.3, USP8.43, USP3.12, USP3.16 and USP8.6 were identified as Arthrobacter nicotianae,? Shewanella alga, Pseudomonas synxantha, Pseudomonas falgida, Pseudovibrio denitrificans and Bacillus aquamaris, respectively. USP3.37 strain was found to amplify gene fragments of non-ribosomal peptide synthetase (NRPS).? This raises the possibility the use of softcoral bacteria as the source of antibacterial compounds for controlling the antifouling in the sea. Therefore, ?this bacterium would be better to select eco-friendly antifouling compounds than the other antibacterial activities.
Co-Authors A B Susanto A. Sabdono Adi Santoso Afriyanto, Rachmat Agung Dhamar Syakti Agus Hardiyanto Agus Sabdono Agus Trianto Awang Surya Wiguna, Awang Surya Bachtiarini, Tiara Ulfa Bahry, Muhammad Syaifudien Boedi Hendrarto Damar Bayu Murti, Damar Bayu Delianis Pringgenies Desy Wulan Triningsih Dietrich G Bengen Dietriech G. Bengen Edi Suhartono Elena Zocchi Endang Supriyantini Fera Nur Idawati Sahara Ferdy S. Rondonuwu Ferdy Semuel Rondonuwu, Ferdy Semuel Ferry F. Karwur Ferry Fredy Karwur Gede Iwan Setiabudi, Gede Iwan Handung Nuryadi HANS- PETER GROSSART Hefni Effendi Hendi Perdian Yunianto Herawati Sudoyo Hidetoshi Urakawa Hidetoshi Urakawal Ika Wulan Santi Iqna Kamila Abfa IRFAN DWIDYA PRIJAMBADA Ita Riniatsih Ita Widowati JOEDORO SOEDARSONO Johannes Hutabarat Khoeri, Miftahuddin M Kouichi Ohwada Kouichi Ohwadai Kumiko Kita-Tsukamoto Kumiko Kita-Tsukamotoz Kustiariyah Tarman Leenawaty Limantara Lia Kusmita Marhaeni, Bintang Meinhard Simon Meutia Samira Ismet Miftahuddin Majid Khoeri Misbakul Munir Muchlisin, Sakti Imam MUJIZAT KAWAROE Mukti K. Diana Putri Nestin E.W. Ningrum Norma Afiati Nuning Vita Hidayati Nurhayati Nurhayati Nurul Ria Arlita Paulus Damar Bayu Murti Pinandoyo, . Pratiwi Sudharmono, Pratiwi Radjasa, Septhy Kusuma Rahmawati, Meris Richardus F Kaswadji Riyanti Riyanti Rr. Citra Permata Rudhi Pribadi S arjito S. Sulistiyani, S. Sahala Hutabarat Sarjito Sarjito Sibero, Mada Triandala Sibero, Mada Triandala Slamet B Prayitno Slamet Budi Prayitno Sri Achadi Nugraheni Sri Sedjati Stöhr, Rudiger Suharsono . Supriharyono ,, Supriharyono Suyono ,, Suyono Tedi Septiadi Tonny Bachtiar Tony Bachtiar TORBEN MARTENS Tri Yuni Atmojo Uswatun Khasanah Vivi Endar Herawati Widyaningsih, Sekar Widyastuti, Yustin Wittriansyah, Khoeruddin Wiwik Astuti Yatnita Parama Cita