Banundari Rachmawati
Dosen Patologi Klinik, Fakultas Kedokteran, Universitas Diponegoro

Published : 9 Documents
Articles

Found 9 Documents
Search

Suplementasi Folate, Kadar Homocysteine, Nitric Oxide dan Petanda Retinopati Diabetik Studi pada Tikus Sprague Dawley Diabetes

MEDIA MEDIKA INDONESIANA 2012:MMI VOLUME 46 ISSUE 2 YEAR 2012
Publisher : MEDIA MEDIKA INDONESIANA

Show Abstract | Original Source | Check in Google Scholar | Full PDF (244.225 KB)

Abstract

Folate supplementation homocysteine, nitric oxide levels and marker for diabetic retinopathy: Study on Sprague Dawley diabetic ratsIntroduction: Diabetic retinopathy (DR) is the leading cause of blindness among diabetes mellitus (DM) patients. Hyperhomocysteinemia (HHcy) is reported to increase the risk for DM complications, however, the underlying mechanism is still unclear. Retina is particularly vulnerable to oxidative stress due to a high demand for oxygen. Hyperglycemia stimulates retinal oxidative stress and increases Nitric Oxide (NO). NO contributes to the regulation of retinal blood vessel function and the occurrence of retinopathy. The administration of folic acid (FA) 0.65 mg/day on HHcy subject, decrease serum Hcy by 42%. This study was aimed to analyze the effect of folate administration on Hcy, NO levels and markers of DR (VEGF serum).Methods: An experimental study using a randomized controlled group pretest posttest design was conducted in UGM Yogyakarta. The total of 40 male Sprague Dawley rats were divided into 5 groups: negative control and the other four group were induced with Streptozotocin 40 mg/kgBW intraperitoneal. Intervention group were given FA 2, 4, 8 ppm through a nasogastric tube for 30 days. Before and after intervention, serum Hcy, NO and VEGF were assessed, and Wilcoxon tests were used to measure the difference ofit.Result: Administration of 8 ppm FA significantly decreased serum Hcy (p=0.043), NO levels (p=0.043) but not for markers of DR.Conclusion: Folic acid supplementation of 8 ppm gives benefits to diabetic rats.Keywords: Diabetic retinopathy, homocysteine, NO, VEGFABSTRAKPendahuluan: Retinopati diabetik (RD) merupakan komplikasi diabetes mellitus yang dapat menjadi salah satu penyebab kebutaan. Hyperhomocysteinemia (HHcy) meningkatkan risiko komplikasi diabetes, namun bagaimana interaksinya masih belum jelas. Retina sangat peka terhadap stres oksidatif karena kebutuhan O2 yang tinggi sedangkan hiperglikemi akan memacu retinal oxidative stres dan peningkatan nitric oxide (NO). NO berperan pada regulasi fungsi pembuluh darah retina dan terjadinya retinopati. Pemberian folic acid (FA) 0,65 mg/hr pada subyek HHcy menurunkan Hcy serum 42%. Tujuan penelitian ini adalah menganalisis pengaruh pemberian folate terhadap kadar Hcy, NO dan petanda RD (VEGF serum)Metode: Penelitian ini bersifat eksperimental dengan rancangan randomized controlled group pretest posttest design. Penelitian dilakukan di LPPT unit IV UGM Yogyakarta. Sampel 40 tikus Sprague Dawley jantan, umur 2-3 bulan, BB 190-275 g dibagi 5 kelompok: kelompok kontrol negatif dan 4 kelompok lain diinduksi dengan STZ 40mg/kgBB intra peritoneal. Kelompok intervensi diberi FA 2, 4, 8 ppm (sonde) selama 30 hari. Kemudian diperiksa kadar Hcy, VEGF, NO serum. Perbedaan parameter sebelum dan sesudah intervensi dianalisis dengan uji Wilcoxon.Hasil: Pemberian FA 8 ppm menurunkan secara bermakna kadar Hcy (p=0,043), NO serum (p=0,043) dan tidak menurunkan petanda RD (VEGF serum) secara bermakna.Simpulan: Pemberian FA 8 ppm bermanfaat pada tikus diabetes.

Pengaruh suplementasi β-carotene terhadap kadar glukosa darah dan kadar malondialdehida pada tikus sprague dawley yang diinduksi Streptozotocin

JURNAL GIZI INDONESIA Vol 2, No 2 (2014)
Publisher : JURNAL GIZI INDONESIA

Show Abstract | Original Source | Check in Google Scholar | Full PDF (217.009 KB)

Abstract

Background : Hyperglycemia on diabetes mellitus can cause increasing of free radicals production. Free radicals caused lipid peroxidation reaction by forming malondialdehyde (MDA). β-carotene has antioxidant activity may inhibit the formation of ROS.Objective : To prove the effect of multilevel doses β-carotene 1 mg/kg BW, 20 mg/kg BW and 20 mg/kg BW on alternate days within 30 days orally supplementation on blood glucose level and MDA level on Sprague Dawley male rats induced by streptozotocin (STZ). Methods : Thirty rats were randomly divided into 5 groups: X1=Negative control/diabetic, X2 (STZ 40 mg/kg BW + BC 1 mg/kg BW), X3 (STZ 40 mg/kg BW + BC 10mg/kg BW), X4 (STZ 40 mg/kg BW + BC 20 mg/kg BW), X5 (technic control/non diabetic). β-Carotene supplementation was given by nasogastric tube on alternate days within thirty days. Blood glucose level was measured by GOD-PAP and MDA level by ELISA with TBARS methods. Data was analized using paired t-test, wilcoxon, one way anova and post hoc bonferroni. Results : there was a significant difference of blood glucose level (p = 0,0001) and MDA level (p = 0,0001) after suplementation β-carotene on alternate days within 30 days orally. β-carotene 10 mg/kg BW was the most effective and efficient dose to lowering blood glucose, while 20 mg/kg BW to lowering MDA level. Conclusion : The multilevel doses β-carotene (1 mg/kg BW, 10 mg/kg BW and 20 mg/kg BW) on alternate days within 30 days orally supplementation can decrease blood glucose and MDA level. β-carotene 10 mg/kg BW is the most effecetive and efficient to decrease blood glucose and β-carotene 20 mg/kg BW to decrease MDA level.

Efek suplementasi β-carotene terhadap kolesterol total, trigliserida dan malondialdehid pada tikus sprague dawley yang diabet

JURNAL GIZI INDONESIA Vol 2, No 2 (2014)
Publisher : JURNAL GIZI INDONESIA

Show Abstract | Original Source | Check in Google Scholar | Full PDF (392.108 KB)

Abstract

Background : diabetes mellitus (DM) is associated with increased total cholesterol and triglyceride, also well characterized by increased malondialdehyde production. β-carotene has antioxidant activity, glycemic and lipid control. Objective : to analyze the effect of β-carotene on total cholesterol, triglyceride and MDA on diabetic Rattus norvegicus sprague dawley. Methods : thirty rats were randomly divided into 5 groups : 1 (STZ), 2 (STZ+β-carotene 1 mg/kg BW), 3 (STZ+β-carotene 10 mg/kg BW), 4 (STZ+β-carotene 20 mg/kg BW), 5 (normal). Streptozotocin induced intraperitoneal 40 mg/kg BW. β-carotene was given by nasogastric tube on alternate days within thirty days. Blood glucose level was measured by GOD-PAP, total cholesterol by CHOD-PAP, triglyceride by GPO and MDA by ELISA with TBARS methods. Hypothesis test used one way anova then followed by post hoc bonferroni to analyze the efficient dose effect. Results : there was a significant difference of total cholesterol (p=0.002) after β-carotene 10 mg/kg BW supplementation on alternate days within 30 days orally. β-carotene 10 mg/kg BW was the most efficient dose to lowering total cholesterol. There were significant differences of triglyceride (p=0.0001) and MDA (p=0.0001) after β-carotene 1, mg/kg BW, 10 mg/kg BW and 20 mg/kg BW supplementation on alternate days within 30 days orally. β-carotene 10 mg/kg BW was the most efficient dose to lowering triglyceride, while 20 mg/kg BW to lowering MDA. Conclusion : β-carotene 10 mg/kg BW is the most efficient dose to lowering total cholesterol and triglyceride. β-carotene 20 mg/kg BW is the most efficient dose to lowering MDA.

Effectiveness of Brown Algae Extract to Reduce Serum Malondialdehyde and Protein Carbonyl Levels in Streptozotocin-Induced Sprague Dawley Rats

Sains Medika Vol 7, No 2 (2016): July-December 2016
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Original Source | Check in Google Scholar | Full PDF (334.148 KB)

Abstract

Introduction: Brown algae (Sargassum duplicatum) contains bioactive compound with natural antioxidant that may potentially decrease lipid peroxidation and protein oxidation process in diabetes mellitus. Objective: to prove the effectiveness of brown algae extract administration (150mg/kgBW; 300mg/kgBW and 450 mg/kGBW respectively) in reducing serum MDA and PCO levels in streptozotocin-induced sprague dawley rat. Methods: This experiment study used the post test only control group design. Twenty eight (28) Sprague dawley rats induced with Streptozotocin (STZ) 40 mg/kgBW (i.p) were divided into four groups (n=7). The first group was diabetic non treated group (control). The second to fourth groups were the diabetic rats which given brown algae extract (150mg/kgBW; 300mg/kgBW and 450 mg/kgBW respectively) for 30 days. Serum MDA and PCO levels were examined by using ELISA method. Data were analyzed using one way ANOVA.Results: There were no significant differences in serum MDA levels among groups (p=0,405). However, serum PCO level in group administered with 450mg/kgBW of brown algae extract decreased significantly compared to control group (p=0.001), group administered with 150mg/kgBW (p=0.001) and 300mg/kgBW (p=0,037). Conclusion: Administration of brown algae extract did not decrease serum MDA levels significantly. Administration of 450mg/kgBW brown algae extract is effective to decrease serum PCO level significantly.

Effectiveness of Brown Algae Extract to Reduce Serum Malondialdehyde and Protein Carbonyl Levels in Streptozotocin-Induced Sprague Dawley Rats

Sains Medika Vol 7, No 2 (2016): July-December (2016)
Publisher : Fakultas Kedokteran, Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Original Source | Check in Google Scholar

Abstract

Introduction: Brown algae (Sargassum duplicatum) contains bioactive compound with natural antioxidant that may potentially decrease lipid peroxidation and protein oxidation process in diabetes mellitus. Objective: to prove the effectiveness of brown algae extract administration (150mg/kgBW; 300mg/kgBW and 450 mg/kGBW respectively) in reducing serum MDA and PCO levels in streptozotocin-induced sprague dawley rat.Methods: This experiment study used the post test only control group design. Twenty eight (28) Sprague dawley rats induced with Streptozotocin (STZ) 40 mg/kgBW (i.p) were divided into four groups (n=7). The first group was diabetic non treated group (control). The second to fourth groups were the diabetic rats which given brown algae extract (150mg/kgBW; 300mg/kgBW and 450 mg/kgBW respectively) for 30 days. Serum MDA and PCO levels were examined by using ELISA method. Data were analyzed using one way ANOVA.Results: There were no significant differences in serum MDA levels among groups (p=0,405). However, serum PCO level in group administered with 450mg/kgBW of brown algae extract decreased significantly compared to control group (p=0.001), group administered with 150mg/kgBW (p=0.001) and 300mg/kgBW (p=0,037).Conclusion: Administration of brown algae extract did not decrease serum MDA levels significantly. Administration of 450mg/kgBW brown algae extract is effective to decrease serum PCO level significantly.

Effect of zinc supplementation on triglyceride and malondialdehyde levels: study on diabetic Wistar rats induced with streptozotocin

Medical Journal of Indonesia Vol 27, No 2 (2018): June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (313.971 KB)

Abstract

Background: Diabetes mellitus is associated with increased blood glucose and triglyceride levels, which can lead to an oxidative stress. Zinc (Zn) is a micronutrient that has antioxidant properties and involved in lipid and glucose metabolism. This study aimed to evaluate the effect of zinc on the levels of fasting blood glucose (FBG), triglycerides (TG), and malondialdehyde (MDA) in male diabetic Rattus norvegicus Wistar rats.Methods: Diabetes was induced by intraperitoneal (i.p.) injection of 40 mg/kg BW streptozotocin (STZ) and confirmed by FBG level higher than 200 mg/dL after 2 weeks. The rats were randomly divided into three groups: control group (STZ), treatment I (STZ + zinc 5 mg/kg BW), and treatment II (STZ + zinc 10 mg/kg BW). Zinc was administered by oral gavage for 30 days. At the end of the experiment, levels of FBG, TG, and MDA were measured. Data were analyzed using paired t-test or Wilcoxon test as appropriate.Results: Supplementation of 5 mg/kg zinc significantly decreased the levels of FBG (pre-intervention: 328.95±70.90 mg/dL, post-intervention: 144.35±34.27 mg/dL, p<0.05), TG (pre-intervention: 252.48±26.30 mg/dL, post-intervention: 147.90±12.18 mg/dL, p<0.05), and MDA (pre-intervention: 12.11±6.46 nm/mL, post-intervention: 4.75±2.34 nm/mL, p<0.05). Moreover, supplementation of 10 mg/kg zinc decreased the levels of TG (pre-intervention: 275.62±56.25nm/mL, post-intervention: 165.58±22.63 nm/mL, p<0.05) and MDA (pre-intervention: 13.08±6.60 nm/mL, post-intervention: 5.08±2.40 nm/mL, p<0.05).Conclusion: Supplementation of zinc significantly reduced the levels of FBG, TG, and MDA in diabetic rats.

PERBEDAAN KADAR UREUM DAN KREATININ PADA ST-ELEVATION MYOCARD INFARCTION (STEMI) DAN NON ST ELEVATION MYOCARD INFARCTION (N-STEMI)

Media Medika Muda Vol 2, No 3 (2017)
Publisher : Faculty of Medicine Diponegoro University

Show Abstract | Original Source | Check in Google Scholar

Abstract

Latar belakang: Infark Miokard Akut (IMA) menyebabkan penurunan curah jantung dan aliran darah menuju ginjal sehingga dapat mengakibatkan fungsi ginjal menurun yang dapat dilihat dari kenaikan ureum dan kreatinin. Kenaikan ureum dan kreatinin dapat sebagai petanda laboratorium untuk membedakan antara ST-elevation  myocard infartion (STEMI) dengan non ST elevation myocard infarction (NSTEMI) sehingga mempercepat penanganan dan menurunkan kasus kematian IMA. Tujuan penelitian ini untuk mengetahui apakah terdapat perbedaan kadar ureum dan kreatinin antara pasien STEMI dengan NSTEMI.Metode: Desain penelitian belah lintang, data sekunder diambil dari rekam medik dari bulan Januari - September 2017 di RSUP Dr. Kariadi. Jumlah sampel 80 pasien, digolongkan dua kelompok yaitu STEMI dan NSTEMI berdasarkan pemeriksaan EKG dan enzim jantung (troponin dan CKMB). Kadar ureum dan kreatinin serum diperiksa dengan alat ADVIA 1800. Data dianalisis dengan program statistik SPSS v.15 menggunakan uji beda mann whitney.Hasil: Ada perbedaan bermakna kadar ureum dan kreatinin antara STEMI dan NSTEMI (p=0,010 dan p=0,003). Hal ini dikarenakan kerusakan otot jantung menyebabkan penurunan curah jantung yang menuju ginjal lebih tinggi pada kasus STEMI dibandingkan NSTEMI.Simpulan: Simpulan: Kadar Ureum dan kreatinin lebih tinggi bermakna pada STEMI. Peningkatan kadar ureum dan kreatinin perlu diperhatikan pada kasus infark miokard. Kata Kunci: Ureum, kreatinin, Infark Miokard Akut, STEMI, NSTEMI

PERBEDAAN KADAR SGOT DAN SGPT ANTARA SUBYEK DENGAN DAN TANPA DIABETES MELLITUS

JURNAL KEDOKTERAN DIPONEGORO Vol 6, No 2 (2017): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Program Studi Kedokteran, Fakultas Kedokteran, Universitas Diponegoro

Show Abstract | Original Source | Check in Google Scholar

Abstract

Latar Belakang: Diabetes Mellitus (DM) masih menjadi sebuah masalah yang serius di kesehatan global terbesar di abad ke-21 ini. Penyakit DM dapat menyebabkan banyak komplikasi salah satunya kelainan penyakit hati. Pemeriksaan kadar SGOT dan SGPT diperlukan untuk meningkatkan kewaspadaan terhadap komplikasi yang didapat dari pasien DM.Tujuan: Membuktikan perbedaan kadar SGOT dan SGPT antara subyek dengan dan tanpa DM.Metode: Jenis penelitian ini adalah observasional analitik dengan pendekatan case control. Data dari semua subyekDM dan subyek tanpa DM di RSUP Dr. Kariadi Semarang yang berusia 20-79 tahun dari tahun 2013 sampai tahun  2016 yang didapatkan dari Instalasi Rekam Medis dan Instalasi Laboratorium  RSUP Dr. Kariadi Semarang. Data dianalisis menggunakan program SPSS.Hasil: Terdapat 25 subyek DM dan 25 subyek tanpa DM. Mayoritas pasien berumur 20-66 tahun. Hasil penelitian didapatkan,rata-rata kadar SGOT pada subyek DM didapatkan45,64 IU/L,kadar SGOT minimum didapatkan 11 IU/L dan maksimum didapatkan 190 IU/L. Rata-rata kadar SGPT pasien DM adalah 54,12 IU/L dengan kadar SGPT minimum 11 IU/L dan maksimum 309 IU/L.  Pada subyek tanpa DM didapatkan rata-rata kadar SGOT 24,32 IU/L, dengan kadar SGOT maksimum adalah 59 IU/L dan minimum 13 IU/L. Rata-rata kadar SGPT pada subyek tanpa DM adalah 42,32 IU/L dengan kadar minimum 23 IU/L dan maksimum 132 IU/L. Berdasarkan analisis bivariat penelitian kadar SGOT dan SGPT antara subyek DM dan subyek tanpa DM menunjukkan nilai p = 0,047 dan p = 0,573.Simpulan: Terdapat perbedaan kadar SGOT antara subyek DM dan subyek tanpa DM dan tidak terdapat perbedaan kadar SGPT antara subyek DM dan subyek tanpa DM.

PERBEDAAN INDEX ERITROSIT PADA PASIEN ANEMIA GAGAL GINJAL KRONIK DAN THALASSEMIA MAYOR

JURNAL KEDOKTERAN DIPONEGORO Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Program Studi Kedokteran, Fakultas Kedokteran, Universitas Diponegoro

Show Abstract | Original Source | Check in Google Scholar

Abstract

Latar Belakang: Pasien GGK dan thalassemia keduanya mengalami anemia mikrositik hipokromik. Beberapa rumus index eritrosit dapat digunakan sebagai parameter penapisan thalassemia. Beberapa contohnya yaitu: Mentzer Index (MI), RBC distribution width index (RDWI), Hisham Index (HI) dan Hameed Index (HA).Tujuan: Membuktikan perbedaan index eritrosit pada pasien gagal ginjal kronik dan thalassemia.Metode: Penelitian ini merupakan penelitian observasional analitik dengan pendekatan belah lintang. Masing-masing populasi berjumlah 40 orang. Data Complete Blood Count (CBC) yang didapat dengan menggunakan alat analisa hematologi otomatis dimasukkan ke dalam rumus MI, RDWI, HI, dan HA. Uji statistik menggunakan uji T tidak berpasangan.Hasil: Rerata MI pada GGK yaitu 28,03 dan pada thalassemia yaitu 20,24 . Rerata RDWI pada GGK yaitu 399,64 dan pada thalassemia yaitu 402,01. Rerata HI pada GGK yaitu 132,39 dan pada thalassemia yaitu 138,49. Rerata HA pada GGK yaitu 14,37 dan pada thalassemia yaitu 23,8. Terdapat perbedaan bermakna antara dua kelompok pada MI (p=0,00) dan HA (p=0,01). Tidak terdapat perbedaan bermakna antara dua kelompok pada RDWI (p=0,917) dan HI (p=0,448).Kesimpulan: Terdapat perbedaan MI dan HA, tetapi tidak terdapat perbedaan pada RDWI dan HI antara 2 kelompok.