Herwandhani Putri
Cancer Chemoprevention Research Center, Fakultas Farmasi, Universitas Gadjah Mada
Ethanolic Extract of Moringa oleifera L. Increases Sensitivity of WiDr Colon Cancer Cell Line Towards 5-Fluorouracil

Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

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For more than four decades, combination chemotherapy (co-chemotherapy) has been employed as a means to increase the effectiveness of chemotherapy regiments. The aim of our research is to investigate the activity of  Moringa oleifera  L. (tanaman kelor) ethanolic extract (MEE) as a co-chemotherapy agent with 5-fluorouracil (5-FU) on WiDr colon cancer cell line. Evaluation of MEE potency as a co-chemotherapy agent with 5-FU was based on cytotoxic activity based on percent cell viability via MTT  assay, and based on apoptosis observation via the double staining method using acrydin orange – ethidium bromide (AE) as the staining reagent.Cytotoxicity evaluation of single treatment using concentrations of 5, 20, 50, 100,125, and 250 µg/ml of MEE reduced cell viability 24 hours post-treatment. 5, 50, and 250 µg/ml of MEE was chosen as the combination concentrations with 1000 µM 5-FU. MTT assay 24 hours and 48 hours post-combination treatment showed significant cell viability reduction in comparison to those of single treatments. Apoptosis observation using the double staining method shows the presence of apoptotic cells 48 hours post combination treatment. MEE is a potential co-chemotherapy agent  by increasing the sensitivity  of WiDr colon cancer cell line towards 5-FU.

Ethanolic Extract of Mangosteen (Garcinia mangostana) Peel Inhibits T47D and Hela Cells Line Proliferation Via Nf-kB Pathway Inhibition

Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

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Effective and selective chemoterapeutic and chemopreventive agent is needed to cure breast and cervical cancers. One of the potential natural material is mangosteen peel (Garcinia mangostana). In this study, we observed cytotoxic effect of ethanolic extract of mangosteen peel (EMP) on HeLa cells line and T47D cells line. The cytotoxic effect was determined using MTT assay. EMP showed cytotoxic effect on T47D cells and HeLa cells with IC50 values of 2.07 μg/ml and 10.58 μg/ml respectively. Molecular docking simulation was done to predict the molecular mechanism of active compund in mangosteen peel extract, α-mangostin, in NFκB pathway which is one of the potential pathway to induce cytotoxicity on T47D and HeLa cells. Docking was done using PLANTS software and the binding score between α-mangostin and proteasom is -78,12, whereas the binding score between α-mangostin and IKK is -86.84. These results showed the possiblity mechanism of mangostin peel extract containing α-mangostin inhibits IKK activation in NFκB pathway. Based on this study, we conclude that mangosteen peel extract is potential to be developed as chemopreventive agent toward cervical and breast cancers.Keywords : Mangosteen peel (Garcinia mangostana), cytotoxic, T47D cells, HeLa cells, NFκB

The Selectivity of Ethanolic Extract of Buah Makassar (Brucea javanica) on Metastatic Breast Cancer Cells

Journal of Agromedicine and Medical Sciences Vol 2, No 1 (2016)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

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Treatment of cancer such as surgery, radiotherapy and chemotherapy has many side effects. Chemopreventive agent is needed to reduce the side effect and increase the effectivity of therapy. The discovery of  cochemopreventive agent should consider on its selectivity to reduce side effects. The selective cochemopreventive agents work effectively in cancer cells and safe for normal cells. Buah Makassar (Brucea javanica) is a natural product that is empirically used for anti-inflammatory and antitumor. The purpose of this study is to determine the cytotoxic effect of ethanolic extract of buah Makassar against 4T1, MCF7, HeLa, and Vero cell lines. The cytotoxic test is performed by MTT assay. The parameter obtained from the cytotoxic test was IC50. Selectivity index is determined from IC50 ratio of cancer cells to normal cells. The results showed that ethanolic extract of buah Makassar has a cytotoxic activity on 4T1, MCF7, HeLa, and Vero cells with IC50 were 49,9±0,83 μg/mL; 107,6±8,14 μg/mL; 228,9±4,16 μg/mL and 395,5± 4,21 μg/mL respectively. It also has high selectivity on 4T1 metastatic breast cancer cell with selectivity index of 7,93. It can be concluded that the ethanolic extract of buah Makassar has potential to be delevoped as cochemopreventive agent especially on metastatic breast cancer. Keywords: Brucea javanica, MTT assay, selectivity index, 4T1, MCF7, HeLa, Vero


UNEJ e-Proceeding 2016: Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UNEJ e-Proceeding

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Breast cancer is one of the most commonmalignancy on woman in Indonesia (Kemenkes RI,2015). Breast cancer patients usually come todoctor in a late stage & the deaths are due to thecomplications of metastasis in vital organs (NationalCancer Institute, 2012). The death case mostprobably happened when cancer is aggresive &distant metastazed. One factor that causingaggresivity & metastasis is presence of HER2 protein.HER2 is found in 25% cases of Ca mammae. Her2status also influences preference of drug for cancertreatment. Besides of antibodi monoklonal &antireseptor of tirosine kinase, doxorubicin is stillused for treatment breast cancer with overexpressHER2 (Nielsen et al., 2009). Long term use ofdoxorubicin causes several side effects, resistanceand toxicity to normal tissues (Smith et al., 2010).Therefore, combining doxorubicin withchemopreventive agent is needed to increaseactivity of doxorubicin, to overcome the drugresistance and to reduce its side effect (Sarkar andLi, 2006). Chemopreventive agent used in thisresearch are temulawak (Curcuma xanthorrhiza) andawar-awar (Ficus septica). The leaves of Ficus septicahave been used to treat colds, fungal and bacterialdiseases, and various cancers. The ethanolic extractof Ficus septica showed a cytotoxic effect on breastcancer T47D cell lines with IC50 value of 13 μg/mL.The extract at 4.88 μg/mL also showed an optimumsynergistic effect in combination with doxorubicin(3.75 nmol) (Pratama et al., 2010). In addition, theextract induced apoptosis and down regulated theexpression of Bcl-2 protein in breast cancer cellsMCF-7 (Sekti et al., 2010). The major components ofC. xanthorrhiza are curcumin and xanthorrhizol.Curcumin suppresses a number of key elements incellular signal transduction pathways pertinent togrowth, differentiation, and malignanttransformation (Kunumakkara et al., 2008). Choi etal. (2005) observed that injection of 0.2-1.0 mg/kgbw xanthorrhizol had an antimetastatic effect in amouse lung metastasis model.It is a general assumption that combinationtreatment is sometimes more beneficial than singlecompounds. The previous study reported thatcombination of doxorubicin and chemopreventiveagent showed synergistic effect in cancer treatment(Lewandowska et al., 2014). The purpose of thisstudy is to prove antimetastasis effect ofcombination of ethanolic extract of rhizome ofCurcuma xanthorrhiza (ECx), ethanolic extract ofleave of Ficus septica (EFs), and doxorubicin solelyand its combination on MCF7-HER2 breast cancercell line through inhibition activity of MMP-9.Cytotoxic assay is used to determine the synergy ofcombinations. Antimetastasis effect was observedthrough inhibition of cancer cell invasion. Gelatinzymography signify the inhibitory activity of MMP-9,which plays an important role in cancer cell invasion.