Dyaningtyas Dewi Pamungkas Putri
Cancer Chemoprevention Research Center Faculty of Pharmacy, Universitas Gadjah Mada

Published : 14 Documents
Articles

Found 14 Documents
Search

Aktivitas Sitotoksik Ekstrak Etanolik Rimpang Temu Kunci (Boesenbergia pandurata) Terhadap Sel Kanker Serviks HeLa dan Sel Kanker Kolon WiDr Handoko, Fransiscus Feby; Maruti, Astrid Ayu; Rivanti, Erlina; Putri, Dyaningtyas Dewi Pamungkas; Meiyanto, Edy
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
Publisher : Majalah Kesehatan Pharmamedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Temu kunci (Boesenbergia pandurata) rhizome showed cytotoxic effect against T47D breast cancer cell line. It contains Panduratin, a chalcone compund, that has been investigated as chemopreventive agent. The exploration of extract of temu kuci as chemopreventive agent was expected to be an alternative for cancer therapy. The aim of this research was to determine the cytotoxic activities of ethanolic extract of temu kunci against HeLa cervix cancer and WiDr colon cancer cell line. The cytotoxic activities of ethanolic extract of temu kunci were tested using MTT assay against HeLa and WiDr cells. The IC50 values were obtained using linier regression equation. The ethanolic extract of temu kunci showed cytotoxic activities on HeLa cervix cancer and WiDr colon cancer cell lines with IC50 at 87 µg/mL and 76 µg/mL, respectively. Low IC50 values (<100 µg/mL) showed that ethanolic extract of temu kunci is potential to be developed as chemoprevention agent on cervix cancer and colon cancer. However, its molecular mecahanism need to be explored.
Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells Ismiyati, Nur; Putri, Dyaningtyas Dewi Pamungkas; Kusumastuti, Siska Andrina; Febriansyah, Rifki
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Eugenia uniflora Lam. is one of herbal products developed for anticancer. The aim of the present study was to identify the antiproliferative effect of ethanolic extract of Eugenia Uniflora Lam. leaves (EEU) on breast cancer cell line T47D. This Research was initiated by extracting the active contents of Eugenia uniflora Lam. leaves by maceration with ethanol 96%. The extract was then analyzed by thin layer chromatography (TLC). Cytotoxic assay of EEU was carried out by using MTT assay. Apoptosis phenomenon was observed with double staining using acridine orange-ethidium bromide. EEU showed cytotoxic effct on T47D cells with IC50 value of 65 μg/ml. Moreover, EEU 50 μg/ml and 100 μg/ml induced apoptosis. TLC examination showed that EEU used in this study contain phenolic, flavonoid, and saponin compounds which were suggested to be responsible for antiproliferative effect. Further molecular mechanism underlying EEU antiproliferative effect needs to be done.Keywords : Eugenia uniflora Lam., T47D cells, antiproliferative, apoptosis
Secang (Caesalpinia sappan L.) Heartwood Ethanolic Extract Shows Activity as Doxorubicin Co-chemotherapeutic Agent by Apoptosis Induction on T47D Breast Cancer Cells Nurzijah, Ika; Putri, Dyaningtyas Dewi Pamungkas; Rivanti, Erlina; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Doxorubicin, primary chemoteurapeutic agent used for breast cancer treatment, is known to have various side effects included multi drug resistance 9MDR) phenomenon. Therefore, exploration of co-chemotherapeutic agent is important to be conducted in order to prevent MDR. Secang (Caesalpinia sappan L.) which contains active compounds brazilin and brazilein, is proven to have activity as anticancer. The aim of this study is to determine the potency of Caesalpinia sappan L.ethanolic extract (CEE) as co-chemotherapeutic agent of doxorubicin and its mechanism through apoptosis induction on T47D breast cancer cells. Caesalpinia sappan L. heartwood powder was macerated with ethanol 70%. The cytotoxic effect of CEE alone and its combination with doxorubicin was analyzed using MTT assay. Apoptosis assay was done by flowcytometry-annexin V method. CEE showed cytotoxic activity on T47D cells with IC50 value of 35 μg/ml, while combinatorial test showed that all of combination doses of CEE and doxorubicin gave synergistic effect. Flowcytometry-annexin V assay proved that treatment of CEE induced apoptosis of doxorubicin. Based on these results, we conclude that Caesalpinia sappan L. heartwood ethanolic extract is potential to be developed as co-chemotherapeutic agent of doxorubicin.Keywords : Caesalpinia sappan L., doxorubicib, apoptosis, T47D cells. 
Ethanolic Extract of Secang (Caesalpinia sappan L.) Wood Performs as Chemosensitizing Agent Through Apoptotic Induction on Breast Cancer MCF-7 Cells Khamsita, Rahmi; Hermawan, Adam; Putri, Dyaningtyas Dewi Pamungkas; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 3, No 3 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Resistance  to  chemotherapy  is  believed  to  cause  treatment  failure  of  the  patient cancer. Secang (Caesalpinia sappan L.) has been proven to possess anticancer activity on some cancer cell lines. The aimed of this study to develop ethanolic extract of secang wood (EES) as  chemosensitizing  agent  through  apoptotic  induction  on  breast  cancer  MCF-7  cells. Extraction  of  secang  was  done  by  using  maceration  with  70  %  ethanol.  Single  and combinatorial treatment of EES and doxorubicin on MCF-7 breast cancer cells were analyzed by using MTT assay to determine the IC50 value and combination index (CI) to evaluate the combinatorial effect. Apoptosis was analyzed with flowcytometry (annexin V).  EES showed a dose-dependent cytotoxicity (IC50 value of 37 µg/ml), while combinatorial treatment showed that  7  concentrations  was  found  to  be  synergist  with  doxorubicin  on  MCF-7  cells. Combinatorial treatment also triggered apoptotic instead of single treatment. Based on this result,  we  conclude  that  ethanolic  extract  of  secang  wood  is  potential  as  chemosensitizing agent in breast cancer.Keyword: Caesalpinia sappan L, MCF-7 cells, doxorubicin, apoptosis.
Combination of Leunca Herb Ethanolic Extract and Doxorubicin Suppresses HeLa Cells’ Growth Sarmoko, Sarmoko; Putri, Dyaningtyas Dewi Pamungkas; Puspitasari, Endah; Anindyajati, Anindyajati; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (572.029 KB) | DOI: 10.14499/indonesianjcanchemoprev2iss3pp281-285

Abstract

Leunca (Solanum nigrum L.)ethanolic extractshowedcytotoxic activity on several cancer cell lines (HepG2, HT-29) and showed anti-proliferative activityon MCF-7 cells. Its application as a combinationagent in chemotherapy will increase the effectivity and reduce the toxicity of chemotherapy. We predict that application of combinatorial chemotherapy in cancer treatment will be more effective and less toxic compared to single treatment. Our research aims to investigate the cytotoxic activitiy of leunca herbs ethanolic extract alone and in combination with doxorubicin on HeLa cell line. MTT assay was conducted to measure the growth inhibitory effect of leunca herbs ethanolic extract and combinatorial treatments. Leunca herb ethanolic extract (5, 50, 250 μg/ml) increased the cytotoxic effect of  doxorubicin compared to doxorubicin alone. The strongest cytotoxic activity resulted from the combination of 250 μg/ml leunca herbs ethanolic extract and 250 nM doxorubicin. Based on our results, leunca herbs ethanolic extract is a potential chemopreventive agent, while its molecular mechanism needs to be explored.Keywords : Leunca herbs ethanolic extract, doxorubicin, HeLa, MTT assay
MCF-7 Resistant Doxorubicin are Characterized by Lamelapodia, Strong Adhesion on Substrate and P-gp Overexpression Putri, Dyaningtyas Dewi Pamungkas; Sarmoko, Sarmoko; Febriansah, Rifki; Puspitasari, Endah; Ismiyati, Nur; Fitriasari, Aditya
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (672.1 KB) | DOI: 10.14499/indonesianjcanchemoprev2iss3pp304-309

Abstract

The prognosis of breast cancer patients is closely associated with the response of tumor cells to chemotherapy agent. Doxorubicin is one of the primary chemotherapeutic agents used for the treatment of breast cancer. Resistance to chemotherapy is believed to cause treatment failure in cancer patients. Furthermore, long time exposure to chemotherapeutic agent induces cancer cells resistance. MCF-7 sensitive cells used as chemoresistance model have overexpression P-gp (P-glycoprotein). Chemoresistance was established by treating MCF-7 cells with 0.5 µg/ml doxorubicin-contained medium for a week. 50% inhibiting concentration (IC50) doxorubicin on MCF-7 cells/DOX were determined using MTT assay. Western blot assay and immunocytochemistry assay was performed to determine the expression of P-gp. Morphological of MCF-7 cell/DOX was changing to become larger and have lamellapodia. IC50 value of doxorubicin was 700 nM on MCF-7/DOX and 400 nM on sensitive MCF-7 cells. The MCF-7/DOX sensitivity to doxorubicin was decreased, shown by 1.5 fold higher IC50 of doxorubicin on MCF-7/DOX compared to MCF-7 sensitive cells. Treatment doxorubicin to sensitive MCF-7 cells leads to the increasing P-gp expression. The P-gp level expression has strong correlation with the low sensitivity of MCF-7/DOX to doxorubicin.Keywords: doxorubicin, resistance cells, sensitive MCF-7 cell
Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells Ismiyati, Nur; Putri, Dyaningtyas Dewi Pamungkas; Kusumastuti, Siska Andrina; Febriansyah, Rifki
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (711.437 KB) | DOI: 10.14499/indonesianjcanchemoprev3iss2pp370-375

Abstract

Eugenia uniflora Lam. is one of herbal products developed for anticancer. The aim of the present study was to identify the antiproliferative effect of ethanolic extract of Eugenia Uniflora Lam. leaves (EEU) on breast cancer cell line T47D. This Research was initiated by extracting the active contents of Eugenia uniflora Lam. leaves by maceration with ethanol 96%. The extract was then analyzed by thin layer chromatography (TLC). Cytotoxic assay of EEU was carried out by using MTT assay. Apoptosis phenomenon was observed with double staining using acridine orange-ethidium bromide. EEU showed cytotoxic effect on T47D cells with IC50 value of 65 µg/ml. Moreover, EEU 50µg/ml and 100µg/ml induced apoptosis. TLC examination showed that EEU used in this study contain phenolic, flavonoid, and saponin compounds which were suggested to be responsible for antiproliferative effect. Further molecular mechanism underlying EEU antiproliferative effect needs to be done.Keywords: Eugenia uniflora Lam., T47D cells, antiproliferative, apoptosis
The Cytotoxic Activity of Solanum Nigrum Ethanolic Extract on Widr Human Colon Cancer Cells Maruti, Astrid Ayu; F., Ilham Augusta; Putri, Dyaningtyas Dewi Pamungkas; Hermawan, Adam; Ikawati, Muthi'
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.206 KB) | DOI: 10.14499/indonesianjcanchemoprev2iss3pp291-294

Abstract

Solanum nigrum L. or Leunca in Indonesia has been traditionally used as a herbal plant, which is believed to have anti-tumor properties, although the mechanism for the activity remains unknown. The resecarch aim to examine the cytotoxic effect of the ethanolic extract of Solanum nigrum on WiDr human colon cancer cells. In this study, we prepared an ethanol extract from herb of Solanum nigrum and investigated the mechanism involved in its growth-inhibitory effect on WiDr human colon cancer cells. Herbs of Solanum nigrum dry powder is extracted with 70% ethanol then added into the WiDr cell culture in 96 wells plate in various concentration : 50, 100, 250, and 500 µg/ml. Cytotoxicity of the Solanum nigrum ethanolic extract  was analyzed with MTT assay on WiDr human colon cancer cell lines. Results from the MTT assay showed WiDr cells was weakly suppressed in the presence of the extract. The result of the assay also showed a very close correlation between the Solanum nigrum extract concentration and the surviving cell numbers which means the extract caused cell death in a dose-dependent fashion in WiDr cancer cells with the IC50 of 359,23 µg/ml. Collectively, the research suggest further studies to explore other chemopreventive possibilites of Solanum nigrum ethanolic extract.Keywords : colon cancer, MTT assay, cytotoxic, WiDr, Solanum nigrum
Secang (Caesalpinia sappan L.) Heartwood Ethanolic Extract Shows Activity as Doxorubicin Co-chemotherapeutic Agent by Apoptotis Induction on T47D Breast Cancer Cells Nurzijah, Ika; Putri, Dyaningtyas Dewi Pamungkas; Rivanti, Erlina; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.23 KB) | DOI: 10.14499/indonesianjcanchemoprev3iss2pp376-383

Abstract

Doxorubicin, primary chemoteurapeutic agent used for breast cancer treatment, is known to have various side effects included multi drug resistance (MDR) phenomenon. Therefore, exploration of co-chemotherapeutic agent is important to be conducted in order to prevent MDR. Secang (Caesalpinia sappan L.) which contains active compounds brazilin and brazilein, is proven to have activity as anticancer. The aim of this study is to determine the potency of Caesalpinia sappan L. ethanolic extract (CEE) as co-chemotherapeutic agent of doxorubicin and its mechanism through apoptosis induction on T47D breast cancer cells. Caesalpinia sappan L. heartwood powder was macerated with ethanol 70%. The cytotoxic effect of CEE alone and its combination with doxorubicin was analyzed using MTT assay. Apoptosis assay was done by flowcytometry-annexin V method. CEE showed cytotoxic activity on T47D cells with IC50 value of 35 µg/ml, while combinatorial test showed that all of combination doses of CEE and doxorubicin gave synergistic effect. Flowcytometry-annexin V assay proved that treatment of CEE induced apoptosis of doxorubicin. Based on these results, we conclude that Caesalpinia sappan L. heartwood ethanolic extract is potential to be developed as co-chemotherapeutic agent of doxorubicin.Keywords : Caesalpinia sappan L., doxorubicin, apoptosis, T47D cells
Cytotoxic Activity and Apoptosis Induction of Ethanolic Extract of Pericarps of Mangosteen (Garcinia mangostana Linn.) on WiDr Cells and Interaction Study of Alpha-mangosteen to IKK and VEGF Based on Molecular Docking Rohmah, Annishfia Lailatur; Amalia, Fikri; Rivanti, Erlina; Putri, Dyaningtyas Dewi Pamungkas; Nurulita, Nunuk Aries
Indonesian Journal of Cancer Chemoprevention Vol 4, No 1 (2013)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (631.54 KB) | DOI: 10.14499/indonesianjcanchemoprev4iss1pp470-476

Abstract

One of the compounds found efficacious as an anti-proliferative on colon cancer is alpha-mangosteen, a xanthon compound that is abundant in pericarp of mangosteen. This study focused to evaluate anticancer activity of the ethanolic extract of pericarp of mangosteen (Garcinia mangostana Linn.) on WiDr colon cancer cells and to observe the affinity of alpha-mangosteen in inhibiting IKK and VEGF. Cytotoxic effect was tested by MTT assay and apoptosis induction was evaluated by double staining method on WiDr colon cancer cells, while interaction between alpha-mangosteen to the receptors was measured by molecular docking. The ethanolic extract was proven to have cytotoxic activity against WiDr colon cancer cells with IC50 of 25 µg/mL. In addition, the observation of apoptosis induction by double-staining method showed that apoptosis associated the cytotoxic effect of ethanolic extract of pericarp of mangosteen (EPM) on WiDr colon cancer cells. Molecular docking showed that active compounds in pericarp of mangosteen could compete with the native ligand of VEGF because the docking score of alpha-mangosteen was almost equal with native ligand. From these results we could be concluded that the cytotoxic effect of EPM to WiDr cells was mediated by cell apoptosis. This extract was potential to be developed as chemopreventive agent.Keywords : Garcinia mangostana Linn., cytotoxic effect, apoptosis, WiDr cell, molecular docking