Diah Puspitosari
Faculty of Medicine, Universitas Padjdajaran-Dr. Hasan Sadikin General Hospital

Published : 6 Documents
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Gonorrhoea Infection Prevalence in Human Immunodeficiency Virus Positive Patients Based on Polymerase Chain Reaction Examination

International Journal of Integrated Health Sciences VOL 2, NO 1, July (2014)
Publisher : International Journal of Integrated Health Sciences

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Abstract

Objective: To determine the prevalence of gonorrhoea infection in male and female HIV positive patients based on polymerase chain reaction (PCR)  examination at the Teratai Clinic, Dr. Hasan Sadikin General Hospital Bandung.Methods: This study was conducted in July, 2012 at the Teratai Clinic, while the PCR examination was performed at the Molecular Biology Laboratory of Microbiology Department, Faculty of Medicine, Universitas Padjadjaran, Bandung. This was a cross-sectional observational study. The subjects were 81 HIV positive patients who were taken in consecutive admission. They underwent history taking and physical examination. Samples were taken from urethral swab in males and cervical swab in females for PCR examination.Results: The PCR examination result was positive for gonorrhea in 36% subjects. From all male subjects participating in the study, 37% were positive while 33% of the female subjects were also positive for gonorrhea. Conclusions: The prevalence of gonorrhoea infection in male and female HIV positive patients at the Teratai Clinic, Dr. Hasan  Sadikin  General Hospital  Bandung is quite high, i.e 37% and 33%, respectively.Keywords: Gonorrhoea, human immunodeficiency virus, polymerase chain reaction, prevalence DOI: 10.15850/ijihs.v2n1.270

Chlamydial Infection Prevalence in Human Immunodeficiency Virus Patients

International Journal of Integrated Health Sciences VOL 1, NO 1, July (2013)
Publisher : International Journal of Integrated Health Sciences

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Abstract

Objective: To determine the prevalence of chlamydial infection in male urethra and female cervix of HIV-positive patients based on PCR assay in Teratai Clinic, Dr. Hasan Sadikin General Hospital Bandung. Methods: A cross sectional observational study with consecutive sampling method, which was conducted in Teratai Clinic during the month of July 2012. All participants underwent history taking, physical examination, and PCR assay for C. trachomatis. Swab samples were obtained from male urethra and female endocervix. Results: Eighty one HIV-positive patients, consisted of 48 males and 33 females were included in this study. The PCR examination for C. trachomatis was positive in 14 of 81 subjects (17.3%), with 10 (20.8%) urethral chlamydial infection in 48 males and four (12.1%) cervical chlamydial infection in 33 females. Conclusions: The study concluded that the prevalence of chlamydial infection in male urethra and female cervix of HIV-positive patients were 20.8% and 12.1%, respectively. Identifying chlamydial infection based on PCR testing should be considered for routine screening test in HIV-positive patients.    Keywords: Chlamydia trachomatis, HIV, prevalence, PCR DOI: 10.15850/ijihs.v1n1.107

Mycoplasma Genitalium Infection Prevalence in Patients with Human Immunodeficiency Virus

International Journal of Integrated Health Sciences VOL 2, NO 1, July (2014)
Publisher : International Journal of Integrated Health Sciences

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Abstract

Objective: To determine the prevalence of Mycoplasma genitalium (M. genitalium) infection in HIV positive patients by PCR examination in Teratai Clinic of Dr. Hasan Sadikin General Hospital, Bandung in order to reduce sexually transmitted diseases, especially M. genitalium infection in HIV positive patients.Methods: This study was a cross-sectional study with consecutive sampling methods. Eighty one HIV positive patients attending the Teratai Clinic of Dr. Hasan Sadikin General Hospital, Bandung were recruited to be the subjects of this study. All subjects underwent history taking, physical examination, and PCR examination for M. genitalium. Specimens were taken from cervical smear in females and first void urine in male.Results: The prevalence of M. genitalium based on the PCR examination in HIV positive patients attended to Teratai Clinic Dr. Hasan Sadikin General Hospital Bandung was 9%. Conclusions: Mycoplasmal infection identification based on PCR examination should be considered for routine screening test to reduce the incidence of sexually transmitted diseases in HIV positive patients.Keywords: Human immunodeficiency virus, Mycoplama genitalium, polymerase chain reaction DOI: 10.15850/ijihs.v2n1.274

Chlamydial Infection Prevalence in Human Immunodeficiency Virus Patients

International Journal of Integrated Health Sciences Vol 1, No 1 (2013)
Publisher : International Journal of Integrated Health Sciences

Show Abstract | Original Source | Check in Google Scholar

Abstract

Objective: To determine the prevalence of chlamydial infection in male urethra and female cervix of HIV-positive patients based on PCR assay in Teratai Clinic, Dr. Hasan Sadikin General Hospital Bandung. Methods: A cross sectional observational study with consecutive sampling method, which was conducted in Teratai Clinic during the month of July 2012. All participants underwent history taking, physical examination, and PCR assay for C. trachomatis. Swab samples were obtained from male urethra and female endocervix. Results: Eighty one HIV-positive patients, consisted of 48 males and 33 females were included in this study. The PCR examination for C. trachomatis was positive in 14 of 81 subjects (17.3%), with 10 (20.8%) urethral chlamydial infection in 48 males and four (12.1%) cervical chlamydial infection in 33 females. Conclusions: The study concluded that the prevalence of chlamydial infection in male urethra and female cervix of HIV-positive patients were 20.8% and 12.1%, respectively. Identifying chlamydial infection based on PCR testing should be considered for routine screening test in HIV-positive patients.    Keywords: Chlamydia trachomatis, HIV, prevalence, PCR DOI: 10.15850/ijihs.v1n1.107

Mycoplasma Genitalium Infection Prevalence in Patients with Human Immunodeficiency Virus

International Journal of Integrated Health Sciences Vol 2, No 1 (2014)
Publisher : International Journal of Integrated Health Sciences

Show Abstract | Original Source | Check in Google Scholar

Abstract

Objective: To determine the prevalence of Mycoplasma genitalium (M. genitalium) infection in HIV positive patients by PCR examination in Teratai Clinic of Dr. Hasan Sadikin General Hospital, Bandung in order to reduce sexually transmitted diseases, especially M. genitalium infection in HIV positive patients.Methods: This study was a cross-sectional study with consecutive sampling methods. Eighty one HIV positive patients attending the Teratai Clinic of Dr. Hasan Sadikin General Hospital, Bandung were recruited to be the subjects of this study. All subjects underwent history taking, physical examination, and PCR examination for M. genitalium. Specimens were taken from cervical smear in females and first void urine in male.Results: The prevalence of M. genitalium based on the PCR examination in HIV positive patients attended to Teratai Clinic Dr. Hasan Sadikin General Hospital Bandung was 9%. Conclusions: Mycoplasmal infection identification based on PCR examination should be considered for routine screening test to reduce the incidence of sexually transmitted diseases in HIV positive patients.Keywords: Human immunodeficiency virus, Mycoplama genitalium, polymerase chain reaction DOI: 10.15850/ijihs.v2n1.274

Gonorrhoea Infection Prevalence in Human Immunodeficiency Virus Positive Patients Based on Polymerase Chain Reaction Examination

International Journal of Integrated Health Sciences Vol 2, No 1 (2014)
Publisher : International Journal of Integrated Health Sciences

Show Abstract | Original Source | Check in Google Scholar

Abstract

Objective: To determine the prevalence of gonorrhoea infection in male and female HIV positive patients based on polymerase chain reaction (PCR)  examination at the Teratai Clinic, Dr. Hasan Sadikin General Hospital Bandung.Methods: This study was conducted in July, 2012 at the Teratai Clinic, while the PCR examination was performed at the Molecular Biology Laboratory of Microbiology Department, Faculty of Medicine, Universitas Padjadjaran, Bandung. This was a cross-sectional observational study. The subjects were 81 HIV positive patients who were taken in consecutive admission. They underwent history taking and physical examination. Samples were taken from urethral swab in males and cervical swab in females for PCR examination.Results: The PCR examination result was positive for gonorrhea in 36% subjects. From all male subjects participating in the study, 37% were positive while 33% of the female subjects were also positive for gonorrhea. Conclusions: The prevalence of gonorrhoea infection in male and female HIV positive patients at the Teratai Clinic, Dr. Hasan  Sadikin  General Hospital  Bandung is quite high, i.e 37% and 33%, respectively.Keywords: Gonorrhoea, human immunodeficiency virus, polymerase chain reaction, prevalence DOI: 10.15850/ijihs.v2n1.270