Rachma Puspitasari
Research Center for Oceanography, Indonesian Institute of Sciences, Jl. Pasir Putih I, Ancol Timur Jakarta Utara 14430

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A PRELIMINARY STUDY ON THE RESPONSE OF AMPHIPHOD GRANDIDIERELLA SP. TO CONTAMINATED SEDIMENT OF JAKARTA BAY Hindarti, Dwi; Puspitasari, Rachma; Arifin, Zainal
Marine Research in Indonesia Vol 35 No 2 (2010)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

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Abstract

A preliminary study on the response of amphipod exposed to contaminated sediments of Jakarta Bay was carried out in October 2009. The objective of the study was to determine the mortality rate of amphipod in response to exposure of contaminated sediments. Seven sediment samples were taken from the bay, i.e, reference site (St. A), and six-contaminated sites (St. B3, B5, C3, C5, D3, and D5). Amphipod (Grandidierella sp.) was collected from reference site. The study showed that average of mortality rate of amphipod ranged between 50.0 and 76.3% at contaminated sediments and 38.3% at reference sediments. There was significant difference on mortality rate of amphipod exposed to sediments of reference site and that of contaminated sites. The mortality of Grandidierella sp. was not strongly correlated with the concentration of Pb and Cu in contaminated sediments. The critical mortality factor of amphipod in this study might be due to variation in grain size of sediments and short period of acclimatization.
Chaetoceros gracilis AS A BIOINDICATOR OF SEDIMENT QUALITY Puspitasari, Rachma; Lestari, Lestari
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 6, No 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Jurnal Ilmu dan Teknologi Kelautan Tropis

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Abstract

Chronic sediment toxicity tests could be used to evaluate the effects of sediment on critical stages in the life of an organism like invertebrate larvae and microalgae. Microalgae play an important role in aquatic ecosystem due to their role as producer in food chain producing organic matter and oxygen through photosynthesis process. This paper aimed to assess sediment quality of Semarang water using chronic sediment toxicity test. Research was conducted by testing 13 sediment samples from Semarang coastal region in August, 2010. Results showed that sediments exposed for 96 hours were still able to stimulate the growth of C. gracilis eventhough the copper concentration was above standard value provided by the CCME. The conclusion from this research was that sediment in Semarang still in good condition and can support growth of C. gracilis as primary producer. Chronic sediment toxicity test using C. gracilis could be combined with heavy metal measurement for sediment quality asessment. Keywords: sediment, bioindicator, diatomae, C. gracilis, chronic toxicity, Semarang
Sensitivitas Nitzschia Sp Terhadap Tembaga dan Kadmium Puspitasari, Rachma; Purbonegoro, Triyoni
Jurnal Segara Vol 15, No 1 (2019): April
Publisher : Pusat Riset Kelautan

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Abstract

Penggunaan organisme uji yang sesuai dengan bahan yang diuji adalah faktor yang sangat penting dalam bioassay. Berbagai organisme uji tropis telah banyak digunakan di Indonesia seperti ikan, bulu babi, dan fitoplankton. Fitoplankton seperti Chaetoceros gracilis, Tetraselmis sp., Isochrysis sp., Pavlova sp., yang hidup di kolom air telah dimanfaatkan dengan baik untuk bioassay. Namun, fitoplankton bentik seperti Nitszchia sp. belum dikenal secara luas. Tulisan ini bertujuan untuk menguji potensi Nitzschia sp. sebagai biota uji sedimen. Parameter yang diteliti adalah kurva pertumbuhan dan sensitifitas terhadap kadmium dan tembaga. Hasil penelitian menunjukkan bahwa pertumbuhan Nitszchia sp. meningkat pesat pada hari keempat hingga hari keenam. Setelah hari keenam, pertumbuhannya cenderung menurun. Kepekaan terhadap toksikan diindikasikan dengan nilai IC50 (Inhibition Concentration) sebesar 0,078 mg/Luntuk tembaga dan 0,26 mg/L untuk kadmium. Kesimpulan dari penelitian ini adalah Nitszchia sp. dapat digunakan sebagai organisme uji untuk bioassay sedimen karena memenuhi persyaratan kepadatan minimum uji serta sensitif terhadap kadmium dan tembaga. Nilai LOEC dan NOEC untuk tembaga berturut-turut sebesar 0,056 dan 0,032 mg/L sedangkan untuk kadmium sebesar 0,18 dan <0,18 mg/L. Nitzschia sp. tiga kali lebih sensitif terhadap tembaga dibandingkan dengan kadmium.
Chaetoceros gracilis AS A BIOINDICATOR OF SEDIMENT QUALITY Puspitasari, Rachma; Lestari, Lestari
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 6, No 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Bogor Agricultural University

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Abstract

Chronic sediment toxicity tests could be used to evaluate the effects of sediment on critical stages in the life of an organism like invertebrate larvae and microalgae. Microalgae play an important role in aquatic ecosystem due to their role as producer in food chain producing organic matter and oxygen through photosynthesis process. This paper aimed to assess sediment quality of Semarang water using chronic sediment toxicity test. Research was conducted by testing 13 sediment samples from Semarang coastal region in August, 2010. Results showed that sediments exposed for 96 hours were still able to stimulate the growth of C. gracilis eventhough the copper concentration was above standard value provided by the CCME. The conclusion from this research was that sediment in Semarang still in good condition and can support growth of C. gracilis as primary producer. Chronic sediment toxicity test using C. gracilis could be combined with heavy metal measurement for sediment quality asessment. Keywords: sediment, bioindicator, diatomae, C. gracilis, chronic toxicity, Semarang
PRELIMINARY STUDY OF LARVAL DEVELOPMENT Oryzias javanicus IN INDONESIA Puspitasari, Rachma; Suratno, .
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 9, No 1 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Bogor Agricultural University

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Abstract

Java medaka Oryzias javanicus potentially developed as test organism, represents the coastal region because it has a high adaptability in freshwater, brackishwater and marine environments. Utilization of it as a test organism has some obstacles such as lack of number of test organisms with same size or age. The fulfillment of number can be solved if the test organism is cultivated exclusively in the laboratory. This study was a preliminary study to get information about suitable salinity for spawning and hatching. Parameter observed were spawning ability in 0 and 20 ppt and hatching rate of egg among 0. 15 and 30 ppt and development of larval fish in 0 ppt. Result indicated that the fish was be able to spawn in 0 and 20 ppt. Eggs were hatched within 9 days in 30 ppt, faster than in freshwater and 15 ppt. In general, O. javanicus was be able to spawn either in freshwater or seawater, but there are differences in the behavior of fish in the laying of egg. Fish will carried their eggs in the abdomen in freshwater, while 20 ppt salinity fish tends to release the eggs. O. javanicus be able to live and lay eggs on freshwater and seawater. Selection of salinity is adjusted to test requirement in egg phase, pascalarva or adult. In general, breeding of fish easier and faster done in fresh water while hatching eggs take place more quickly in high salinity.Keywords: Indonesia, Java Medaka, Oryzias javanicus, test organism
Copper and Cadmium Toxicity to Marine Phytoplankton, Chaetoceros gracilis and Isochrysis sp. Suratno, Suratno; Puspitasari, Rachma; Purbonegoro, Triyoni; Mansur, Dieni
Indonesian Journal of Chemistry Vol 15, No 2 (2015)
Publisher : Universitas Gadjah Mada

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Abstract

In Copper (Cu) based antifouling (AF) paints Cu was largely used as booster biocide after organotin was banned. Cu is micronutrient which is important in photosynthesis process because Cu is an essential metal as component of enzyme and electron transport chain. But in certain dosage, Cu could be toxic to marine organism. Chaetoceros gracilis and Isochrysis sp. are dominant microalgae in aquatic ecosystem. In this study the effect of Cu and Cadmium (Cd) on two marine microalgae, C. gracilis and Isochrysis sp. were compared. Toxicity test was based on American Standard for Testing Material (ASTM). IC50-96 h of Cd as reference toxicant was 2,370 mg.L-1 for C. gracilis and 490 mg.L-1 for Isochrysis sp. IC50-96 h of Cu to growth of C. gracilis was 63.75 mg.L-1 and Isochrysis sp. was 31.80 mg.L-1. Both Cd and Cu were inhibited growth of microalgae. Based on IC50-96 h value, it could be concluded that Cu was more toxic than Cd. Toxicity of Cu was 37 times stronger than Cd for C. gracilis and 15 times for Isochrysis sp. It was estimated that at concentration 10 mg.L-1 Cu does not show observable effect (NOEC) to C. gracilis and 5 mg.L-1 to Isochrysis sp. The lowest observable effect of Cu (LOEC) to C. gracilis was at concentration 17 mg.L-1 and 10 mg.L-1 for Isochrysis sp.
A PRELIMINARY STUDY ON THE RESPONSE OF AMPHIPHOD GRANDIDIERELLA SP. TO CONTAMINATED SEDIMENT OF JAKARTA BAY Hindarti, Dwi; Puspitasari, Rachma; Arifin, Zainal
Marine Research in Indonesia Vol 35, No 2 (2010)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Original Source | Check in Google Scholar | Full PDF (9313.793 KB)

Abstract

A preliminary study on the response of amphipod exposed to contaminated sediments of Jakarta Bay was carried out in October 2009. The objective of the study was to determine the mortality rate of amphipod in response to exposure of contaminated sediments. Seven sediment samples were taken from the bay, i.e, reference site (St. A), and six-contaminated sites (St. B3, B5, C3, C5, D3, and D5). Amphipod (Grandidierella sp.) was collected from reference site. The study showed that average of mortality rate of amphipod ranged between 50.0 and 76.3% at contaminated sediments and 38.3% at reference sediments. There was significant difference on mortality rate of amphipod exposed to sediments of reference site and that of contaminated sites. The mortality of Grandidierella sp. was not strongly correlated with the concentration of Pb and Cu in contaminated sediments. The critical mortality factor of amphipod in this study might be due to variation in grain size of sediments and short period of acclimatization.
ASSESSING CONTAMINATION LEVEL OF JAKARTA BAY NEARSHORE SEDIMENTS USING GREEN MUSSEL (PERNA VIRIDIS) LARVAE Cordova, Muhammad Reza; Purbonegoro, Triyoni; Puspitasari, Rachma; Hindarti, Dwi
Marine Research in Indonesia Vol 41, No 2 (2016)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

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Abstract

Indication of accumulation of heavy metal in sediments will lead to problem for shore and sea ecosystems, biota living in that area and human’s health. The research is aiming to analyse the toxicity of sediment from thirty one locations in Jakarta Bay using Perna Viridis. High amount in larval abnormality found in sediments from the area near mainland and estuary are an indication of high influence of waste from anthropogenic activities from Jakarta mainland. These areas are down stream of Cengkareng Drain, Kamal River, Grogol River and Cakung River, area around sea transportation and North Jakarta Integrated Industrial Area. Sediment of Jakarta Bay, especially coming from estuary area and near to the land area causes an increase in green mussel larval abnormality. This indicates the high of waste influence from anthropogenic activity from Jakarta land area.
SEDIMENT CONTAMINANT AND TOXICITY IN KELABAT BAY, BANGKA BELITUNG PROVINCE Hindarti, Dwi; Arifin, Zainal; Puspitasari, Rachma; Rochyatun, Endang
Marine Research in Indonesia Vol 33, No 2 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

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Abstract

Sediment of 16 sites in the Kelabat Bay were monitored for metal contamination and toxicity in 2006. Two patterns of metal contaminants distribution in the sediment were observed. Higher concentrations of lead and copper were found in March than those in July, and so was in the inner bay than those observed in the outer bay, while different pattern was observed for cadmium concentration. The highest lead concentrations were observed at site 7 (inner bay) both in March and July. Although elevated levels of cadmium, lead and copper were observed in sediments, they were still in the tolerable level for marine life. Sediment of the bay was also evaluated for its toxicity using marine phytoplankton, Chaetoceros gracilis and sea urchin, Tripneustes gratila. Results of the tests reveal that no toxic effects of sediment to the growth of phytoplankton were observed, but to sea urchin fertilization of the sediment may have toxic effect.
HEAVY METAL CONDITION IN KRAMAT KEBO ESTUARY, WEST JAVA, INDONESIA as HABITAT of Oryzias Javanicus Puspitasari, Rachma; Lestari, Lestari
Jurnal Segara Vol 14, No 2 (2018): Agustus
Publisher : Pusat Riset Kelautan

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Abstract

Nowadays, contamination monitoring in Indonesia still focused on traditional method like only depend on measurement of chemical and physical parameter.  In addition, the use of organism, known as bioindicator brings some advantages such as knowing about habitat alteration and relationship between organism and environment. Fish from genus Oryzias had been known in Malaysia and Japan as a sensitive bioindicator. One of species, O. javanicus, was found in Kramat Kebo estuary, West Java, Indonesia. This preliminary study aims to determine metal distribution in water and sediment in Kramat kebo estuary, as a habitat of O. javanicus. It is also a preliminary study to assess possibility of O. javanicus as a bioindicator of heavy metal contamination in coastal area.  Sampling was conducted on March and September, 2014 and analysis of total metal was done by Atomic Absorbance Spectrophotometry. Result showed that metal in water still below the threshold value by Ministry of Environment. Metal in sediment analyzed by Sediment Quality Guidelines Quetiont (SQG-Q) index, showed that all stations has SQG-Q value < 0.1, which is means that Kramat Kebo estuary is unimpacted area. We conclude for this preliminary study that existence of O. javanicus were related to less polluted of heavy metal in estuary and it suitable for bioindicator of uncontaminated heavy metal area.