Bambang S. Purwoko
Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor, Jl. Raya Darmaga, Bogor 16680

Published : 79 Documents
Articles

Characterization of Doubled Haploid Derived from Anther Culture for New Type Upland Rice Herawati, Reny; Purwoko, Bambang S.; Dewi, Iswari S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 3 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Anther culture is one of tissue culture methods which can be applied to plant breeding programs in order to accelerate the process of obtaining pure lines. The successful development of rice varieties is highly dependent on genetic diversity and desirable traits. To obtain the genetic variability of doubled haploid lines through anther culture techniques, anther F1 or F2 were used as explants sources. The objectives of the study were to select and characterize doubled haploid lines of upland rice having the characters of new plant type, and to study the genetic variability and agronomic characters of tested doubled haploid lines. A total of 58 doubled haploid lines, and four parents i.e. Fatmawati, SGJT-28, SGJT-36, and Way Rarem were used in this study. The experiment used completely randomized design with three replications. Results showed that the characters of the doubled haploid lines vary considerably. Selection of the character i.e. number of productive tillers, number of filled grain per panicle, and percentage of empty grain was more effective to be selected because they were well correlated to weight of grain per hill, possessed high heritability values, and have wide genetic variability. Based on productive tillers number, number of filled grains per panicle, fertility, weight of 1,000 grains, and weight of grains per hill, the lines of P3-26, P3-27, P3-28, P4-45, P5-50, P6-103, P6-105,  P3-120, P3-134, P3-135, P3-150, P3-158, P3-248, P3-249, P6-271, P6-272, P6-274, P6-276, and  P6-295 were potential for further selection for new type of upland rice.   Keywords:  variability, characters, doubled haploid, new plant type, upland rice
Karakteristik Daun Jarak Pagar (Jatropha curcas L.) dan Hubungannya dengan Fotosintesis Raden, Ince; Purwoko, Bambang S.; Hariyadi, ,; Ghulamahdi, Munif; Santosa, Edi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 2 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of this research was to study the morphology and physiology of jatropha leaves and its relation to photosynthesis. Research was conducted using Randomized Complete Block Design with single factor,  i.e., leaf ages at the canopy with ten replications. The results showed that jatropha had phyllotaxy 5/13 with  angular divergence 1380. In early growing leaf enlarged sharply and then constant after 9 week, remaining green for 14 weeks.  Chlorophyll a, b, and total achieved maximum at 9 week, i.e., 0.45 g/cm2, 0.19 g/cm2, and 0.62 g/cm2, respectively. Photosynthesic rate was maximum at 6 week, i.e., 8.99 µmol/m2/s, while number of  stomata was maximum at 9 week (289.47 mm2). This finding implies that leaf number 11 to 13 or age 6 week after emergence could be used as reference for photosynthetic evaluation.   Key words:  Jatropha curcas L., leaf arrangement, photosynthetic, reference leaf
Pertumbuhan Bibit Tanaman Jarak Pagar (Jatropha curcas L.) pada Berbagai Kedalaman dan Posisi Tanam Benih Santoso, Bambang B.; Purwoko, Bambang S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Germination and growth at seedling stage are critical phases in plant life cycle, especially for physic nut (Jatropha curcas L.) usually grown in a dry land. Therefore seedling preparation plays an important role in nursery stock production. Position and depth of seed sowing affected the germination process and further seedling growth and development. A Completely Randomized Design with two factors was used in the experiment, i.e., depth of seed sowing (1, 2, 3, 4, and 5 cm) and seed position (seed with micropyle at the bottom, face-down, and layback). The result showed that position and depth of seed sowing affected the germination and early growth and development of seedling. The effect of seed position on growth and development of seedling persisted until the end of the experiment when the seedlings were two months old. Face-down position with 2 - 3 cm depth represented the best position and depth for seedling growth.   Key words:  Early seedling growth, micropyle, face-down position, layback position
Pengaruh Alelopati Jarak Pagar (Jatropha curcas L.) terhadap Perkecambahan Benih Jagung, Tomat dan Padi Gogo Raden, Ince; Purwoko, Bambang S.; Santosa, Edi; Hariyadi, ,; Ghulamahdi, Munif
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

  The objective of this research was to evaluate the effect of extract of leaves, seed and root of physic nut on the germination of corn, tomato, and upland rice. Research was conducted by using Complete Randomized Block Design with two factors, i.e., plant organ (leaves, seed and root) and species (corn, tomato and upland rice). The results showed that extract of 36 g dried organ/200 ml water (0.18 g/ml water) affected seed germination of corn, tomato, and upland rice. Extract of leaves tended to reduce percentage of corn seed germination (55.30% vs 70.68%), and extract of root to reduce germination of tomato and upland rice, 52.70% and 68.67%, compared to control 76% and 84%, respectively.   Key words:  Plant organ extract, physic nut, germination  
Evaluasi Beberapa Ekotipe Jarak Pagar (Jatropha curcas L.) untuk Toleransi Cekaman Kekeringan Lapanjang, Iskandar; Purwoko, Bambang S.; Hariyadi, ,; Budi R, Sri Wilarso; Melati, Maya
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 3 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Drought stress in crop  occurs due to imbalance between water supply and demand.  Crop responses to drought stress  depend on their ecotypes.  This study was aimed at examining the tolerance of the physic nut ecotype to drought stress in inceptisol. This study was conducted at Silviculture greenhouse, Forestry Faculty IPB, from September to December 2007. A Completely Randomized Design with two factors was used in the experiment, i.e. water content (80, 60, and 40 % of field capacity) and physic nut ecotypes (Palu, NTB, IP-A, and IP-P) The results showed that (1) drought reduced stem diameter (31.4%), root length (65.49%) and leaf area (72.73%), and decreased plant dry weight (74.83%),(2) tolerance level of the evaluated physic nuts on the drought stress were moderate tolerance (IP-1A, NTB,  Palu) and sensitive (IP- Pakuwon).   Key words:  physic nut, drought stress, tolerance
Keragaan Varietas dan Galur Padi Tipe Baru Indonesia dalam Sistem Ratun Susilawati, ,; Purwoko, Bambang S.; l Aswidinnoor, Hajria; Santosa, Edi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 3 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Eighteen Indonesian new plant type (NPT) of rice varieties and lines were evaluated in a ratoon system. The results showed that ratooning ability was related to the vegetative growth prior to harvest, which gave high ability ratoon growth.  New plant type varieties and several lines of rice had better vegetative growth and produced higher ratoon than other genotypes.  Ratoons started to develop 2-7 days after harvest, with  an average number of leaf 2-4 per tiller.  Number of productive tillers of ratoon ranged from 6.0 to 30.0 per hill, with  number of grain per panicle ranged from 38.0 to 228.2, and grain weight per hill of ratoon 10.4 to 31.2 g, dependent on genotypes. Maturity of ratoon was also dependent on genotypes.  On average, maturity was 68 days after harvest of the main crop.  Grouping of ratooning ability based on hierarchical cluster analysis resulted in three groups, namely nine genotypes had high potential, five genotypes had medium potential, and four genotypes had low potential.  This study indicated that ratoon is potential to increase rice planting index in Indonesia.   The genotypes with high ratoon ability need to be evaluated for agronomic performance in the field.   Keywords:  new plant type, rice, ratooning  ability
PENGARUH BEBERAPA PERLAKUAN PASCA PANEN DAN SUHU PENYIMPANAN TERHADAP KUALITAS DAN DAYA SIMPAN BUAH PISANG CAVENDISH (Musa (GRUP AAA, SUBGRUP Cavendish)) Purwoko, Bambang S.; Juniarti, Diah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 26, No 2 (1998): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

The objective of this research was to determine the effect of prestorage infiltration of CaCl2, infiltration of spermidine, plastic wrapping and waxing on the maintenance of banana shelflife and qualities at different storage temperatures. Prestorage infiltration of Cavendish banana (Musa Cavendishii) with CaCl2 inhibited fruit softening, the increase of sugar content in peel color index. Plastic wrapping could inhibit the increase of weight loss, pulp peel ratio, peel color index, and the decrease of appearance score. Treatment of fruit with spermidine infiltration store in cool temperature could maintain banana shelflife until 27.8 days or 8.1 days longer than control.
Regenerasi Tanaman pada Kultur Antera Padi: Pengaruh Persilangan dan Aplikasi Putresin Dewi, Iswari S.; Purwoko, Bambang S.; Aswidinnoor, Hajrial; Somantri, Ida H.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 2 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The efficient use of anther culture in crop improvement depends on the success of plant regeneration techniques and the recovery of sufficient number of either green haploid plants that may be artificially or spontaneously doubled yielding homozygous diploid pure lines in first generation.  Putrescine, one of growth regulator polyamines, was known as essential factor in embryogenesis on various plant tissues.  The objective of this research was to study the effect of crosses and putrescine application on certain stages of culture most efficient in increasing green plant regeneration.  The experiment was arranged factorially. The first factor was reciprocal crosses of Taipei 309 to Asemandi and Asemandi to Taipei 309.  The second factor was application of putrescine consisted of control (M0: N6 induction medium and MS regeneration medium); M1: N6 induction medium,  MS regeneration medium + 10-3 M putrescine;  M2: N6 induction medium + 10-3 M putrescine, MS regeneration medium;  and M3: N6 induction medium + 10-3 M putrescine, MS regeneration medium + 10-3 M putrescine.  The results indicated that the addition of 10-3 M putrescine into callus induction and plant regeneration media was the best in increasing number and percentage of responding anther, callus formation, responding calli, green plants and number of total plants.  Rice anther culture efficiency, which was indicated by ratio of green plant to responding calli and percentage of green plant to number of anther innoculated, was also increased by the addition of 10-3 M putrescine. At that best treatment maternal effect showed in number of calli producing green plantlets, number of green plantlets, and percentage of green plant to number of anther innoculated. F1 from Taipei309/Asemandi was better  than F1 from Asemandi/Taipei30.   Key words :  Rice, anther culture, putrescine, green plant, reciprocal crosses
Pengaruh Tinggi Pangkasan Batang Utama dan Jumlah Cabang Primer yang Dipelihara terhadap Produksi Minyak Jarak Pagar (Jatropha curcas L.) Raden, Ince; Purwoko, Bambang S.; Hariyadi, ,; Ghulamahdi, Munif; Santosa, Edi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 2 (2009): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of this research was to study shoot architecture in relation to growth, increase production and seed oil content through various stem pruning and number of primary branches. This research was conducted using Randomized Complete Block Design with single factor. It consisted of ten treatments, i.e., K=kontrol, T20= height of stem pruning at 20 cm from soil and without control on number of primary branches, T20-2=height of stem pruning at 20 cm from soil and two primary branches, T20-3= height of stem pruning at 20 cm and three primary branches, T30= height of stem pruning at 30 cm and without control on number of primary branches, T30-2= height of stem pruning at 30 cm and two primary branches, T30-3=height of stem pruning 30 cm and three primary branches, T40=height of stem pruning at 40 cm and without control on number of  primary branches, T40-2=height of stem pruning at 40 cm and two primary branches, and T40-3=height of stem pruning 40 cm and three primary branches. The results showed that stem pruning increased number of primary branches. The highest seed oil content and seed production was achieved T40 and T30-3 treatments i.e., 323.81 g/plant or 0.810 ton/ha and 320.61 g/plant or 0.802 ton/ha with seed oil yield 244.56 kg/ha and 276.61 kg/ha, respectively.   Key words : Jatropha curcas L., seed oil content, pruning, primary branches
Pewarisan Sifat Toleransi Padi Sawah (Oryza sativa L) Terhadap Cekaman Suhu Rendah Limbongan, Yusuf L.; Aswidinnoor, Hajrial; Purwoko, Bambang S.; Trikoesoemaningtyas, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 2 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

One of the  definite successful of rice breeding programs for increasing yield and tolerance to environmental stress condition is selection method.  Selection method will be effectively if it is supported by a perfect knowledge of genetics character inheritance.  The objectives of this study is to investigate the information of inheritance pattern adapted of rice towards low temperature stress condition.  The experiment was conducted in both Sesean rural area, Toraja regency, South Sulawesi on 1500 m above sea levels, an mean temperature 15 oC, type of soil is Podzolic and at Bogor Agriculture Institute.  This experiment is done in January 2006 until July 2007.  The result showed that tolerance parental grew well and higher yield than sensitive parental.  There were no significant differences between F1 and F1 reciprocal for all characters.  Tolerance parental is more superior than sensitive parental in all characters have been tested.  Mean of  F1 generation is in between tolerance parental and sensitive parental.  BCP1 has spheres wider than parentals and F1 generation, but BCP2 smaller than parentals and F1 generation.  F2 generation is spheres wider than the average of both parentals.  The m [d] [h] genetic model is the most suitable for all characters.  Except, date of flowering and weight of filled rice where appropriate models is m [d] [h] [l].  Broad sense and narrow sense heritability is range between low to high.  Based on the degree of dominant, additive effect is higher than dominant effect. The negative F value reached on date of flowering and fur lenght; and others characters, F value is positive.    Key words: Low temperaure stress tolerance, backcross, reciprocal,  broad sense heritability, narrow sense heritability