Bambang S Purwoko
Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor, Kampus Darmaga, Bogor 16680

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Penyaringan Ketahanan Jalur Haploid Ganda Padi Gogo Hasil Kultur Antera Terhadap Penyakit Blas Daun Bakhtiar, Bakhtiar; Purwoko, Bambang S; Amir, Mukelar
Jurnal Agrista Vol 13, No 2 (2009): Volume 13 Nomor 2 Agustus 2009
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Abstract

Screening of Doubled Haploid Lines Derived from Anther Culture of Upland Rice to Leaf Blast DiseaseABSTRACT. Rice blast disease caused by Pyricularia grisea (cooke) Sacc, is one of limiting factors to increase upland rice production. Many resistant varieties have been developed, but they have become susceptible within a few years after their release. Breeding for blast disease resistance should be done continuously. This study was conducted in Laboratory and Screenhouse at ICABIOGARD, Bogor. Preparation of rice seedlings and inoculums, inoculation technique and scoring system were done according to standard procedures established by IRRI. The evaluation of doubled haploid lines showed that SGJT3, SGJT16, SGJT28, SGJT29, SGJT34, SGGM5, SGGM8, GRGM9, and GRJT12 lines were resistant to leaf blast disease race 173, 033, and 001.
PENGUJIAN VARIETAS DAN DOSIS PUPUK UNTUK MENINGKATKAN PERTUMBUHAN DAN HASIL RATUN-PADI DI SAWAH PASANG SURUT ., Susilawati; Purwoko, Bambang S
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 15, No 1 (2012): Maret 2012
Publisher : Jurnal Pengkajian dan Pengembangan Teknologi Pertanian

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Test of Rice Varieties and Fertilizer Dose for Increasing Yield of Rice Ratoon in Tidal Swamp Land. The main cause of low productivity of rice in the tidal swamp land is a high level of soil acidity and toxicity of pyrite. To improve the productivity of rice per season in tidal swamp land is to establish the ratoon. The experiment was conducted in type B tidal swamp land, Dadahup A-2 village, Kapuas District, Central Kalimantan. The objectives of this study were (a) to determine the effect of doses of fertilizers combined with water-logging after cutting, (b) to obtain technology information to enhance the ratoon yield, and (c) to produce recommendations of the farming system in tidal swamp land. The experiment was arranged in a split plot design with three replications. The main plots were five rice varieties, i.e. IR42, Batanghari, Ciherang, Intani-2, and Batang Samo, while subplots were two levels of doses fertilizers, i e. half doses of the main crops (N1 = 75 kg Urea, 50 kg SP-36 and 50 kg of KCl per hectare) and one quarter doses of the main crops (N2 = 37,5 kg of Urea, 25 kg SP-36 and 25 kg of KCl per ha). The results of the experiment indicated that application of fertilizers with a half dose of the main crops increased the grain per panicle, number of filled grain and yield, that was higher than a quarter doses of the main crops. Batang Samo variety was the most responsive to fertilization with higher doses, while Intani-2 variety was responsive to a lower dose of the fertilizer. Batanghari was superior variety with the highest yield of ratoon both a half doses fertilization and a quarter of the main crops. Based on the farming analysis, the ratooning system is very feasible in the tidal swamp lands because it can increasing productivity ± 2.4 – 2.7 t/ha. Salah satu upaya untuk meningkatkan produktivitas padi di lahan pasang surut adalah dengan memanfaatkan ratun. Penerapan budidaya padi dengan sistem ratun, dapat memberikan tambahan produksi 40-60% per musim tanam, hemat input, biaya, tenaga dan waktu. Suatu penelitian dilaksanakan di lahan pasang surut tipe B, Dadahup A-2, Kabupaten Kapuas, Kalimatan Tengah, mulai bulan Juni 2007 sampai Januari 2008. Penelitian bertujuan untuk : (a) mengetahui pengaruh dosis pupuk dan penggenangan air setelah panen tanaman utama, (b) mendapatkan informasi teknologi untuk meningkatkan potensi ratun, dan (c) menghasilkan rekomendasi usahatani dengan sistem ratun di lahan pasang surut. Percobaan menggunakan rancangan petak terpisah dengan tiga ulangan. Petak utama terdiri atas lima varietas padi, yaitu : IR42, Batanghari, Ciherang, Intani-2, dan Batang Samo. Anak petak terdiri atas dua takaran dosis pemupukan, yaitu : setengah dari dosis tanaman utama (N1 = 75 kg Urea, 50 kg SP-36 dan 50 kg KCl per ha) dan seperempat dari dosis tanaman utama (N2 = 37.5 kg Urea, 25 kg SP-36 dan 25 kg KCl per hektar). Ratun yang dipupuk dengan perlakuan N1 menghasilkan jumlah gabah per malai, jumlah gabah isi dan hasil yang lebih tinggi dibandingkan dengan perlakuan N2. Varietas Batang Samo paling responsif tehadap pemupukan dengan dosis tinggi, sedangkan varietas Intani-2 paling responsif terhadap pemupukan dengan dosis rendah. Varietas Batanghari merupakan varietas terbaik yang mampu memberikan hasil ratun tertinggi, baik pada perlakuan N1 maupun N2. Usahatani dengan sistem ratun layak diusahakan di lahan pasang surut karena mampu memberikan tambahan poduksi  2,4 – 2,7 t/ha.
Penentuan Metode Terbaik Uji Kalium untuk Tanaman Tomat Pada Tanah Inceptisols Izhar, Luthfi; Susila, Anas D; Purwoko, Bambang S; Sutandi, Atang; Mangku, Iwayan
Jurnal Hortikultura Vol 23, No 3 (2013): September 2013
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.086 KB) | DOI: 10.21082/jhort.v23n3.2013.p218-224

Abstract

Penelitian tentang studi analisis kalium tanah dan aplikasi pupuk kalium pada budidaya tomat pada tanah Inceptisols dilakukan di Kebun Percobaan dan Rumah Kaca di Cikabayan, Institut Pertanian Bogor, dari Bulan April hingga November 2011. Tujuan penelitian untuk mendapatkan metode ekstraksi kalium tanah yang terbaik guna menentukan dosis pupuk kalium pada budidaya tomat pada tanah Inceptisols. Penelitian menggunakan rancangan acak kelompok dengan perlakuan pemberian pupuk kalium pada beberapa tingkat dosis, yaitu 0, ¼, ½, ¾, dan 1X, dimana nilai X ialah 608,6 kg K O kg/ha dengan empat ulangan. Perlakuan pemupukan kalium diterapkan pada 3 bulan sebelum penanaman tomat. Analisis korelasi dilakukan antara kandungan K tanah dan pertumbuhan tanaman di dalam rumah kaca menggunakan media inkubasi berasal dari tanah setelah diberi perlakuan. Uji kalium tanah menggunakan lima metode ekstraksi, yaitu metode HCl 25%, NH422) OAc 1 M pH 7, Mehlich I, Truog, dan Morgan Vanema. Hasil penelitian menunjukkan perbedaan yang nyata antara pengaruh perlakuan pupuk K terhadap parameter tinggi tanaman, jumlah daun, dan diameter batang tomat. Bobot segar biomassa dan bobot kering tomat juga menunjukkan perbedaan pengaruh yang nyata antarperlakuan. Nilai korelasi tertinggi ditunjukkan pada metode pengekstrak Truog melalui parameter bobot kering dan basah relatif tanaman (r = 0,7). Dengan demikian, uji K tanah menggunakan metode Truog dapat digunakan sebagai metode ekstraksi yang paling tepat untuk menganalisis unsur hara kalium dalam rangka penyediaan rekomendasi pemupukan K pada budidaya tomat pada tanah Inceptisols.
Konservasi In Vitro Tanaman Jeruk Besar (Citrus maxima (Burm.) Merr.) Kultivar Srinyonya Menggunakan Osmotikum dan Retardan Dewi, Iswari S; Jawak, Gani; Roostika, Ika; Sabda, Muhammad; Purwoko, Bambang S; Adil, Widiati H
Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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In Vitro Conservation of Pomelo (Citrus maxima (Burm.)Merr.) cv Srinyonya Using Osmoticum and Retardant.Iswari S. Dewi, Gani Jawak, Ika Roostika, M. Sabda,Bambang S. Purwoko, and Widiati H. Adil. Pomelo is anunderutilized citrus fruit with a potential for commercialization.Only some cultivars have been conserved ex situ, suchas in home yards or in botanical gardens. Such collectionsare vulnerable to biotic and abiotic hazards. The goal of theexperiment was to study the effect of osmoticum (sorbitol)and retardant (ancymidol) on in vitro growth of pomelo.Four-leaf in vitro shoots of pomelo cultivar Srinyonya wereused as plant materials. Murashige-Skoog (MS) medium wasused as the basal medium for the culture. The trial wasarranged in a completely randomized design with threereplications. The treatments consisting of MS + sorbitol (0,20, 40, and 60 g/l) and MS + ancymidol (0, 1, 3, and 5 mg/l).The results indicated that based on plant height, number ofnew leaves, and visual plant architecture, sorbitol treatmentsfrom 20-60 g/l retard the growth of the pomelo plant significantly.On the other hand, ancymidol did not inhibit thepomelo growth significantly, but it was a suitable osmoticumfor improvement of in vitro plant vigor, increasing greencolor of leaf, and increasing root initiation. Leaf senescenceof in vitro plants cultured on media containing sorbitol 40and 60 g/l began 20 week after storage. The best medium forconservation of pomelo cv Srinyonya was MS + 20 gsorbitol/l.
Pengaruh TDZ terhadap induksi embrio somatik sagu (Metroxylon sagu Rottb.) pada tiga metode kultur berbeda (Effect of TDZ on the somatic embryo induction of sago palm (Metroxylon sagu Rottb.) in three different culture methods) Riyadi, Imron; EFENDI, Darda; PURWOKO, Bambang S; SANTOSO, Djoko
E-Journal Menara Perkebunan Vol 86, No 1 (2018): April, 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.376 KB) | DOI: 10.22302/iribb.jur.mp.v1i1.258

Abstract

AbstractA right combination of cytokinin is able to support the process of callus differentiation to somatic embryo formation in plant somatic embryogenesis. Liquid culture application could increase the efficiency of in vitro culture process on plants. This research aimed to determine the best concentration of TDZ combined with kinetin for callus differentiation to  somatic embryo of sago palm on three culture methods. Plant material used was embryogenic callus derived from tips meristem culture from sucker of Alitir sago palm. Callus was cultured on modified MS media added with: 0.0, 0.1, 0.5 and 1.0 mg/L TDZ combined with 0.5 mg/L kinetin for 12 weeks with subcultures every 6 weeks. Three culture methods used were suspension, temporary immersion system (TIS), and solid media. There were 12 treatments with 4 replicates. The results showed that the highest number of somatic embryos was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin in 6 weeks (167.3 embryos/flask) and 12 weeks (389.2 embryos/flask) with its fresh weight of 18.4 g and 29.1 g, respectively. The highset survival rate in final culture (12 weeks) was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin (100%). The shortest time for somatic embryos expression was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin in two weeks after culture. Histological analysis of early-stage somatic embryos showed the presence of dense and compact cellular arrangements which formed growth spot axis for shoot or SAM (shoot apical meristem) and root or RAM (root apical meristem) that connected each other. [Key words: culture method, embryogenic callus, Metroxylon sagu Rottb., kinetin, sago palm, TDZ]   AbstrakAplikasi kombinasi sitokinin yang tepat dapat mendorong proses diferensiasi kalus membentuk embrio somatik pada proses embriogenesis somatik tanaman. Penggunaan metode kultur cair dapat meningkatkan efisiensi proses kultur in vitro tanaman. Penelitian ini bertujuan untuk menentukan konsentrasi TDZ terbaik dikombinasikan dengan kinetin dalam proses diferensiasi kalus membentuk embrio somatik tanaman sagu pada tiga metode kultur. Bahan tanam penelitian  berupa kalus embriogenik tanaman sagu asal kultur meristem pucuk dari anakan sagu jenis Alitir. Kalus dikulturkan pada media modifikasi dengan penambahan  TDZ dengan konsentrasi 0,1; 0,5; dan 1,0 mg/L dikombinasikan dengan kinetin 0,5 mg/L selama 12 minggu yang disubkultur pada umur 6 minggu. Metode kultur yang digunakan terdiri atas tiga macam yaitu: kultur suspensi, sistem perendaman sesaat (SPS) dan media padat. Perlakuan terdiri atas 12 kombinasi perlakuan dengan empat ulangan. Hasil penelitian menunjukkan bahwa rerata jumlah embrio somatik tertinggi dicapai pada perlakuan metode kultur SPS dengan TDZ 1,0 mg/L baik pada umur kultur 6 minggu (167,3 buah) maupun umur 12 minggu (389,2 buah). Rerata bobot segar tertinggi juga diperoleh pada perlakuan metode kultur SPS dengan TDZ 1,0 mg/L  pada umur kultur 6 minggu (18,4 g) dan  12 minggu (29,1 g). Rerata daya hidup kultur akhir (12 minggu) tertinggi  sebesar 100% diperoleh pada perlakuan SPS. Induksi embrio somatik  tercepat yakni setelah  dua minggu diperoleh pada  metode kultur SPS dengan TDZ 1,0 mg/L dikombinasikan dengan kinetin 0,5 mg/L. Analisis histologi embrio somatik stadium awal  menunjukkan adanya susunan sel yang rapat dan kompak yang menyusun semacam poros atau berkas titik tumbuh tunas atau SAM (shoot apical meristem) maupun akar atau RAM (root apical mersitem) yang saling terhubung.[Kata kunci: kalus embriogenik, metode kultur, kinetin, TDZ, sagu, Metroxylon sagu]
Pengaruh TDZ terhadap induksi embrio somatik sagu (Metroxylon sagu Rottb.) pada tiga metode kultur berbeda (Effect of TDZ on the somatic embryo induction of sago palm (Metroxylon sagu Rottb.) in three different culture methods) Riyadi, Imron; EFENDI, Darda; PURWOKO, Bambang S; SANTOSO, Djoko
E-Journal Menara Perkebunan Vol 86, No 1 (2018): April, 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.376 KB) | DOI: 10.22302/iribb.jur.mp.v1i1.258

Abstract

AbstractA right combination of cytokinin is able to support the process of callus differentiation to somatic embryo formation in plant somatic embryogenesis. Liquid culture application could increase the efficiency of in vitro culture process on plants. This research aimed to determine the best concentration of TDZ combined with kinetin for callus differentiation to  somatic embryo of sago palm on three culture methods. Plant material used was embryogenic callus derived from tips meristem culture from sucker of Alitir sago palm. Callus was cultured on modified MS media added with: 0.0, 0.1, 0.5 and 1.0 mg/L TDZ combined with 0.5 mg/L kinetin for 12 weeks with subcultures every 6 weeks. Three culture methods used were suspension, temporary immersion system (TIS), and solid media. There were 12 treatments with 4 replicates. The results showed that the highest number of somatic embryos was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin in 6 weeks (167.3 embryos/flask) and 12 weeks (389.2 embryos/flask) with its fresh weight of 18.4 g and 29.1 g, respectively. The highset survival rate in final culture (12 weeks) was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin (100%). The shortest time for somatic embryos expression was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin in two weeks after culture. Histological analysis of early-stage somatic embryos showed the presence of dense and compact cellular arrangements which formed growth spot axis for shoot or SAM (shoot apical meristem) and root or RAM (root apical meristem) that connected each other. [Key words: culture method, embryogenic callus, Metroxylon sagu Rottb., kinetin, sago palm, TDZ]   AbstrakAplikasi kombinasi sitokinin yang tepat dapat mendorong proses diferensiasi kalus membentuk embrio somatik pada proses embriogenesis somatik tanaman. Penggunaan metode kultur cair dapat meningkatkan efisiensi proses kultur in vitro tanaman. Penelitian ini bertujuan untuk menentukan konsentrasi TDZ terbaik dikombinasikan dengan kinetin dalam proses diferensiasi kalus membentuk embrio somatik tanaman sagu pada tiga metode kultur. Bahan tanam penelitian  berupa kalus embriogenik tanaman sagu asal kultur meristem pucuk dari anakan sagu jenis Alitir. Kalus dikulturkan pada media modifikasi dengan penambahan  TDZ dengan konsentrasi 0,1; 0,5; dan 1,0 mg/L dikombinasikan dengan kinetin 0,5 mg/L selama 12 minggu yang disubkultur pada umur 6 minggu. Metode kultur yang digunakan terdiri atas tiga macam yaitu: kultur suspensi, sistem perendaman sesaat (SPS) dan media padat. Perlakuan terdiri atas 12 kombinasi perlakuan dengan empat ulangan. Hasil penelitian menunjukkan bahwa rerata jumlah embrio somatik tertinggi dicapai pada perlakuan metode kultur SPS dengan TDZ 1,0 mg/L baik pada umur kultur 6 minggu (167,3 buah) maupun umur 12 minggu (389,2 buah). Rerata bobot segar tertinggi juga diperoleh pada perlakuan metode kultur SPS dengan TDZ 1,0 mg/L  pada umur kultur 6 minggu (18,4 g) dan  12 minggu (29,1 g). Rerata daya hidup kultur akhir (12 minggu) tertinggi  sebesar 100% diperoleh pada perlakuan SPS. Induksi embrio somatik  tercepat yakni setelah  dua minggu diperoleh pada  metode kultur SPS dengan TDZ 1,0 mg/L dikombinasikan dengan kinetin 0,5 mg/L. Analisis histologi embrio somatik stadium awal  menunjukkan adanya susunan sel yang rapat dan kompak yang menyusun semacam poros atau berkas titik tumbuh tunas atau SAM (shoot apical meristem) maupun akar atau RAM (root apical mersitem) yang saling terhubung.[Kata kunci: kalus embriogenik, metode kultur, kinetin, TDZ, sagu, Metroxylon sagu]