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Journal : Jurnal Ilmu Pertanian

PENGARUH GIBERELIN TERHADAP KARAKTER MORFOLOGI DAN HASIL BUAH PARTENOKARPI PADA TUJUH GENOTIPE TOMAT (SOLANUM LYCOPERSICUM L.) Setiawan, Agus Budi; Murti, Rudi Hari; Purwantoro, Aziz
Jurnal Ilmu Pertanian Vol 18, No 2 (2015)
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.456 KB) | DOI: 10.22146/ipas.6521

Abstract

Giberelin merupakan zat pengatur tumbuh yang berperan dalam pertumbuhan dan perkembangan buah tomat. Buah partenokarpi dapat diinduksi dengan menggunakan giberelin. Penelitian ini bertujuan untuk menentukan respon tujuh genotipe tomat terhadap GA3 terkait karakter fruit set, ukuran, dan hasil buah tomat. Penelitian ini dilakukan di Green House UPTD Balai Pengembangan Perbenihan Tanaman Pangan dan Hortikultura, Dinas Pertanian Pemerintah Daerah Istimewa Yogyakarta serta Laboratorium Genetika dan Pemuliaan Tanaman, Fakultas Pertanian, UGM mulai bulan Oktober 2014 hingga Februari 2015. Penelitian menggunakan rancangan faktorial 7 genotipe x 2 konsentrasi GA3 yang disusun dalam rancangan acak kelompok lengkap dengan 3 blok. Kluster bunga dengan bunga pertama fase 12 yang tidak dikastrasi disemprot GA3 dengan interval 3 hari sekali sebanyak 6 kali. Hasil penelitian menunjukkan bahwa B78 merupakan genotipe yang responsif terhadap GA3dengan ditandai dengan peningkatan jumlah lokul menjadi 6 (lokul/buah) danmengalami penurunan fruit set sebesar 81,96% serta ukuran buah yang menurun secara nyata dibandingkan dengan buah berbiji. Genotipe yang tanggap terhadap aplikasi GA3 untuk menginduksi buah partenokarpi dengan hasil dan ukuran buah yang bagus adalah Gamato 1 ditandai dengan penurunan bobot buah per tandan yang relatif kecil yaitu 28,38% serta buah partenokarpi yang dihasilkan memiliki ukuran panjang dan diameter buah yang masih jauh lebih besar (41,68 mm dan 46,11 mm) dibandingkan genotipe lainnya. Buah partenokarpi A65, Gamato 3, A175, Gamato 5 dan Kaliurang 206 mengalami penurunan ukuran (panjang, diameter, dan ketebalan daging buah) serta penurunan hasil buah tomat dibandingkan dengan buah berbiji.
GENETIC DIVERSITY OF CROTON (CODIAEUM VARIEGATUM (L.) RUMPH.EX A. JUSS) AND ITS OFFSPRING BASED ON RAPD MARKERS Asniawati, Mufit Daryatun; Purwantoro, Aziz
Jurnal Ilmu Pertanian Vol 4, No 2 (2019): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5061.12 KB) | DOI: 10.22146/ipas.40899

Abstract

Croton (Puring) is a native  plant of Indonesia which has varied leaf shapes and colors. The diversity of croton increase through hybridization. The information on genetic diversity and relationship between parent and its offspring of crotons is very limited. This study aims to analyze the genetic diversity of cultivar Mawar (MW) and Walet (W) as parent compare to their offspring i.e, Black Marlet (BM), Kingkit 1 (KA), Kingkit 2 (KB), and Kamaratih (KM) using RAPD markers. This study used DNA extraction from the fresh leaf of six cultivars. The next steps were DNA quantification, primary optimization, DNA amplification with PCR, and electrophoresis. Statistical analysis was carried out using Genalex software. A total of 40 primers were screened, out of which 10 were selected for the analysis of genetic diversity. A total of 106 polymorphic bands were generated, ranging from 130 to 1850 bp. The results of RAPD analysis showed that Mawar as female parent had the highest polymorphic bands percentage of 69.01%, while Walet as male parent and its offspring ranged from 31.15 % to 43.94%. The genetic distance of the offspring with Walet ranged from 0.176 to 0.234 and genetic distance of the offspring with Mawar ranged from 0.314 to 0.372. It was indicated that all of offspring were closer to the male parent.
TELOMERE LENGTH DETERMINATION BASED ON DIFFERENT SEX AND LEAF DEVELOPMENT OF SNAKE FRUIT (SALACCA ZALACCA GART. VOSS.) REVEALED BY POLYMERASE CHAIN REACTION Indhirawati, Rima; Purwantoro, Aziz
Jurnal Ilmu Pertanian Vol 3, No 3 (2018): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2131.873 KB) | DOI: 10.22146/ipas.36172

Abstract

Telomere has special roles at the ends of eukaryotic chromosomes that consisting of repeated DNA sequences. It protects chromosomes and DNA from damage. The plant sex and the leaf development may change in telomere length. Snake fruit (Salacca zalacca GART. VOSS.) is dioecious plants that have female and male organs on separate plants. The aim of this research is to determine the telomere length of snake fruit from different plant sex and the leaf development. In this research, we observed telomere length in snake fruit (female and male plants) using Polymerase Chain Reaction (PCR) procedure. The results from this study showed that telomere lengths of male and female are equal. Telomere lengths in the leaf development showed that younger leaves of both male and female leaves are longer compared to older and dried leaves.
THE EFFECT OF DRYING AND STORAGE ON THE QUALITY OF SHALLOT (ALLIUM CEPA L. AGGREGATUM GROUP) BULBS Sri Lestari, Rohimah Handayani; Sulistyaningsih, Endang; Purwantoro, Aziz
Jurnal Ilmu Pertanian Vol 3, No 3 (2018): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.835 KB) | DOI: 10.22146/ipas.34203

Abstract

Post-harvest handling in shallot such as drying of bulbs can influence its quality during and after storage. The objective of this study was to determine the impact of drying and storage treatment on the quality of shallot bulbs during 12 weeks of storage . The study was carried out in Samiran hamlet, Parangtritis village, Bantul district, Special Region of Yogyakarta and Crop Science Laboratory of the Faculty of Agriculture, Universitas Gadjah Mada, from June until November 2016. It was arranged in factorial randomized complete block design (RCBD) and consisted of two factors. The first was the drying treatments: drying the bulbs on the field and on woven bamboo nets both plastic covered and uncovered. The second was the storage treatments: storing the bulbs in the farmer?s warehouse (31,030C±0,04 and RH of 60,50%±0,28), in air-conditioned room (22,40oC± 0,02 and RH of 61,60%±0,09), and at room temperature (30,47oC±0,03 and RH of 60,50%±0,12). Each treatment combination was replicated three times as blocks. The results showed that water content of all treatments were changing followed by fluctuating of the total soluble solid throughout the storage period while bulb firmness tended to decrease. Bulbs which were stored in air-conditioned rooms showed the highest percentage of sprouted bulbs, vigor index and germination rate than other treatments. Meanwhile, drying treatment did not give significant influence.          
DIVERSITY ANALYSIS OF 15 HIBISCUS ACCESSION BASED ON RAPD MARKER Sari, Miranda Ferwita; Purwantoro, Aziz
Jurnal Ilmu Pertanian Vol 3, No 2 (2018): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2477.064 KB) | DOI: 10.22146/ipas.33211

Abstract

Genus Hibiscus consist of 300 species in tropical and subtropical regions. Indonesia has many species of Hibiscus genus such as Hibiscus rosa-sinensis, kenaf, rosella, waru, sharon, and others. These species have similar flower morphology despite their different benefits. Flower morphology can be use as morphological marker characters to identify the genetic relationship in one genus of Hibiscus. However, morphological markers are less accurate because they are strongly influenced by the environment, requiring quite amount of time, and showing limited and inconsistent diversity. These limitations make researcher chooses molecular markers that are considered more accurate because the material used is plant DNA. One of the most used molecular marker is Random Amplified Polymorphic DNA (RAPD). This study used DNA extraction from the leaf of 15 Hibiscus genus. The next steps were DNA quantification, temperature and primer optimization, DNA amplification with PCR, electrophoresis with agarose gel, and data analysis. The result showed 10 primers operon used for Hibiscus to produce many polymorphic bands. Analysis result showed a high diversity in control population (Hibiscus cannabinus (K1), Hibiscus sabdariffa (K2) Hibiscus mutabilis (K3), Hibiscus syriacus (K4), Hibiscus schizopetalus (K5)) while in Hibiscus rosa-sinensis, the diversity was low especially on the genotypes of flowers with the same color. Molecular marker is a right way to identify the diversity in a population. Control group which consist of Hibiscus cannabinus, Hibiscus sabdariffa, Hibiscus mutabilis, Hibiscus syriacus and Hibiscus schizopetalus has high heterozygosity means control group has high diversity. From the PCA result, grouping in control plants is based on their age (perennial and annual), while in Hibiscus rosa-sinensis the grouping is based on the flowers color. Hibiscus rosa-sinensis is cross pollinated plants caused the position stigma higher than anthers, its prooved by this experiment that varians within population Hibiscus rosa-sinensis higher than among population.