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Journal : Majalah Kedokteran Bandung

Deteksi Aging pada Perempuan Berdasarkan Status Antioksidan Winarsi, Hery; Yuniati, Alice; Purwanto, Agus
Majalah Kedokteran Bandung Vol 45, No 3 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Aging diinisiasi oleh terakumulasinya radikal bebas. Antioksidan yang diketahui dapat mengendalikan reaktivitas radikal bebas diasumsi sebagai anti-aging. Penelitian ini bertujuan untuk mengetahui usia awal terjadi aging pada perempuan berdasarkan status antioksidan. Penelitian ini bersifat observasional analitik yang dirancang secara purposive random sampling dilakukan di Purwokerto pada tahun 2008 dengan melibatkan 34 perempuan usia balita, anak-anak, remaja, dewasa, tua dan lansia, yang sehat, dan tinggal di Purwokerto. Status antioksidan diketahui berdasarkan aktivitas enzim superoksida dismutase (SOD), katalase, dan glutation peroksidase (GSHPX) eritrosit serta kadar malondialdehid (MDA) plasma responden. Data diuji menggunakan analysis of varians (ANOVA). Status antioksidan perempuan sehat dari usia balita hingga lansia adalah prima yang ditunjukkan oleh tingginya aktivitas SOD berkisar 1.469+3,58–2.009+4,12 U/mg; katalase 20,37+0,01–31,45+0.04 UI/mg; GSH-PX 79,03+0,0–225,2+0,04 μmol/g protein eritrosit; didukung oleh kadar MDA yang rendah dengan kisaran 3.134+2,56 –3.185+3,06 pmol/mL plasma. Secara umum, penurunan status antioksidan terjadi pada usia dewasa (24–<45 tahun), sehingga pada usia tersebut para perempuan mulai memerlukan tambahan suplemen antioksidan guna menghambat laju proses aging dalam tubuhnya. [MKB. 2013;45(3):141–6] Aging Detection in Female Based on Antioxidant StatusAging is initiated by the accumulation of free radicals. Antioxidants which were known to control the reactivity of free radicals can be assumed as an anti aging. This study aimed to determine the age of the onset of aging in female based on antioxidant status. The analytical observational study with purposively random sampling design was conducted in Purwokerto in the year of 2008 with 34 females included toddlers, children, adolescents, adults, old, and elderly who were healthy, and live in Purwokerto. Antioxidant status was known by enzyme activities of superoxide dismutase (SOD), catalase, gluthathion peroxidase (GSH-PX) in the erythrocytes and malondialdehyde (MDA) levels in the plasma. Data were tested by analysis of varians (ANOVA). Antioxidant status in healthy female from toddlers to the elderly were prime as indicated by the high SOD, catalase, and GSH-PX activities that ranged 1,469+3.58–2,009+4.12 U/mg; 20.37+0.01–31.45+0.04 UI/mg; 79.03+0.01–225.2+0.04 μmol/g protein of erythrocytes, respectively; supported by low levels of MDA that ranged from 3,134+2.56–3,185+3.06 pmol/mL plasma. In general, the decrease in antioxidant status occurred in adults, so at that age female began to need additional antioxidant supplements in order to inhibit the rate of aging processes in the body. [MKB. 2013;45(3):141–6] DOI: http://dx.doi.org/10.15395/mkb.v45n3.143
Aktivitas Enzim Superoksida Dismutase, Katalase, dan Glutation Peroksidase Wanita Penderita Sindrom Metabolik Winarsi, Hery; M. Wijayanti, Siwi P.; Purwanto, Agus
Majalah Kedokteran Bandung Vol 44, No 1 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rendahnya status antioksidan endogenus memicu perkembangan kondisi sindrom metabolik (SM). Penelitian ini bertujuan untuk mengeksplorasi aktivitas enzim antioksidan superoksida dismutase (SOD), katalase, dan glutation peroksidase (GSH-PX) dalam plasma wanita penderita sindrom metabolik. Penelitian dilakukan periode Maret–November 2010, dengan responden penelitian adalah 30 wanita yang memiliki kriteria sebagai berikut: kadar guladarah >normal, body mass index >25 kg/m2, hipertrigliseridemia, high-density lipoprotein (HDL) rendah, usia >40 tahun, dan tinggal di Purwokerto. Seleksi responden dimulai melalui survey di poliklinik internis Rumah Sakit Umum Margono Soekarjo Purwokerto. Kepada calon responden dijelaskan tentang tujuan penelitian, kondisi SM, dan perkembangannya. Calon responden yang bersedia bergabung diminta menandatangani informed consent, diukur berat badan, tinggi badan, dan tekanan darahnya. Diambil sampel darahnya 2 mL, intravena, ditentukan kadar glukosa darah, trigliserida, dan HDL. Bagian plasma, diuji aktivitas enzim SOD, katalase, dan GSH-PX. Data dipresentasikan sebagai rata-rata + standard error. Status antioksidan wanita penderita sindrom metabolik rendah, ditunjukkan oleh aktivitas SOD 379,3 Unit/mg protein, katalase 6,42 UI/mg protein, dan GSH-PX 804,9 nmol/g protein, serta kadar malondialdehid (MDA) sebesar 2.943,4 pmol/mL. Karena itu responden perlu asupan kaya antioksidan. Simpulan, status antioksidan peroksidase sama rendah yang ditunjukkan oleh rendahnya aktivitas enzim SOD, katalase, dan glutation peroksidase. [MKB. 2012;44(1):7–12].Kata kunci: Glutation peroksidase, katalase, sindrom metabolik, superoksida dismutase The Activity of Superoxide Dismutase, Catalase and Glutathione Peroxidase Enzymes in Metabolic Syndrome WomenThe low endogenous antioxidant status induced development of metabolic syndrome (MetS) condition. The aim of this research was to explore superoxide dismutase (SOD), catalase (Cat), and glutathione peroxidase (GSH-PX) plasma activity on metabolic syndrome women plasma. Respondents were thirty women with blood glucose level >normal, body mass index >25 kg/m2, hypertrigliceridemic, low level cholesterol-high-density lipoprotein (HDL), the age >40 years and live in Purwokerto. Respondents’ selection was started by a survey at internist polyclinic of Margono Soekarjo General Hospital Purwokerto. They were motivated and given an explanation about aim of the research, their diseases and development risk who were willing to be respondents were asked to sign the informed consent. Then, their body weight, height and blood pressure were measured. Their blood sample of 2 mL were taken intravenously and tested for blood glucose, triglyceride and HDL levels. Plasma was tested for the activities of SOD, Cat and GSH-PX enzymes. Presented data was mean + standard error. The antioxidant status of MetS women were low, showed on the activities of SOD, catalase, and glutathione peroxidase enzyme were 379.3 Unit/mg protein, 6.42 UI/mL, 804.9 nmol/g protein respectively, and malondialdehyde (MDA) level was 2,943.4 pmol/mL. Therefore, they need food enrich antioxidant. In conclusions, peroxidase antioxidant status was similar shown by low SOD, catalase and glutathione peroxidase enzyme activity, [MKB. 2012;44(1):7–12].Key words: Catalase, glutathione peroxidase, metabolic syndrome, superoxide dismutase DOI: http://dx.doi.org/10.15395/mkb.v44n1.75
Aktivitas Enzim Superoksida Dismutase, Katalase, dan Glutation Peroksidase Wanita Penderita Sindrom Metabolik Winarsi, Hery; M. Wijayanti, Siwi P.; Purwanto, Agus
Majalah Kedokteran Bandung Vol 44, No 1 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rendahnya status antioksidan endogenus memicu perkembangan kondisi sindrom metabolik (SM). Penelitian ini bertujuan untuk mengeksplorasi aktivitas enzim antioksidan superoksida dismutase (SOD), katalase, dan glutation peroksidase (GSH-PX) dalam plasma wanita penderita sindrom metabolik. Penelitian dilakukan periode Maret–November 2010, dengan responden penelitian adalah 30 wanita yang memiliki kriteria sebagai berikut: kadar guladarah >normal, body mass index >25 kg/m2, hipertrigliseridemia, high-density lipoprotein (HDL) rendah, usia >40 tahun, dan tinggal di Purwokerto. Seleksi responden dimulai melalui survey di poliklinik internis Rumah Sakit Umum Margono Soekarjo Purwokerto. Kepada calon responden dijelaskan tentang tujuan penelitian, kondisi SM, dan perkembangannya. Calon responden yang bersedia bergabung diminta menandatangani informed consent, diukur berat badan, tinggi badan, dan tekanan darahnya. Diambil sampel darahnya 2 mL, intravena, ditentukan kadar glukosa darah, trigliserida, dan HDL. Bagian plasma, diuji aktivitas enzim SOD, katalase, dan GSH-PX. Data dipresentasikan sebagai rata-rata + standard error. Status antioksidan wanita penderita sindrom metabolik rendah, ditunjukkan oleh aktivitas SOD 379,3 Unit/mg protein, katalase 6,42 UI/mg protein, dan GSH-PX 804,9 nmol/g protein, serta kadar malondialdehid (MDA) sebesar 2.943,4 pmol/mL. Karena itu responden perlu asupan kaya antioksidan. Simpulan, status antioksidan peroksidase sama rendah yang ditunjukkan oleh rendahnya aktivitas enzim SOD, katalase, dan glutation peroksidase. [MKB. 2012;44(1):7–12].Kata kunci: Glutation peroksidase, katalase, sindrom metabolik, superoksida dismutase The Activity of Superoxide Dismutase, Catalase and Glutathione Peroxidase Enzymes in Metabolic Syndrome WomenThe low endogenous antioxidant status induced development of metabolic syndrome (MetS) condition. The aim of this research was to explore superoxide dismutase (SOD), catalase (Cat), and glutathione peroxidase (GSH-PX) plasma activity on metabolic syndrome women plasma. Respondents were thirty women with blood glucose level >normal, body mass index >25 kg/m2, hypertrigliceridemic, low level cholesterol-high-density lipoprotein (HDL), the age >40 years and live in Purwokerto. Respondents’ selection was started by a survey at internist polyclinic of Margono Soekarjo General Hospital Purwokerto. They were motivated and given an explanation about aim of the research, their diseases and development risk who were willing to be respondents were asked to sign the informed consent. Then, their body weight, height and blood pressure were measured. Their blood sample of 2 mL were taken intravenously and tested for blood glucose, triglyceride and HDL levels. Plasma was tested for the activities of SOD, Cat and GSH-PX enzymes. Presented data was mean + standard error. The antioxidant status of MetS women were low, showed on the activities of SOD, catalase, and glutathione peroxidase enzyme were 379.3 Unit/mg protein, 6.42 UI/mL, 804.9 nmol/g protein respectively, and malondialdehyde (MDA) level was 2,943.4 pmol/mL. Therefore, they need food enrich antioxidant. In conclusions, peroxidase antioxidant status was similar shown by low SOD, catalase and glutathione peroxidase enzyme activity, [MKB. 2012;44(1):7–12].Key words: Catalase, glutathione peroxidase, metabolic syndrome, superoxide dismutase DOI: http://dx.doi.org/10.15395/mkb.v44n1.75
Deteksi Aging pada Perempuan Berdasarkan Status Antioksidan Winarsi, Hery; Yuniati, Alice; Purwanto, Agus
Majalah Kedokteran Bandung Vol 45, No 3 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Aging diinisiasi oleh terakumulasinya radikal bebas. Antioksidan yang diketahui dapat mengendalikan reaktivitas radikal bebas diasumsi sebagai anti-aging. Penelitian ini bertujuan untuk mengetahui usia awal terjadi aging pada perempuan berdasarkan status antioksidan. Penelitian ini bersifat observasional analitik yang dirancang secara purposive random sampling dilakukan di Purwokerto pada tahun 2008 dengan melibatkan 34 perempuan usia balita, anak-anak, remaja, dewasa, tua dan lansia, yang sehat, dan tinggal di Purwokerto. Status antioksidan diketahui berdasarkan aktivitas enzim superoksida dismutase (SOD), katalase, dan glutation peroksidase (GSHPX) eritrosit serta kadar malondialdehid (MDA) plasma responden. Data diuji menggunakan analysis of varians (ANOVA). Status antioksidan perempuan sehat dari usia balita hingga lansia adalah prima yang ditunjukkan oleh tingginya aktivitas SOD berkisar 1.469+3,58–2.009+4,12 U/mg; katalase 20,37+0,01–31,45+0.04 UI/mg; GSH-PX 79,03+0,0–225,2+0,04 μmol/g protein eritrosit; didukung oleh kadar MDA yang rendah dengan kisaran 3.134+2,56 –3.185+3,06 pmol/mL plasma. Secara umum, penurunan status antioksidan terjadi pada usia dewasa (24–<45 tahun), sehingga pada usia tersebut para perempuan mulai memerlukan tambahan suplemen antioksidan guna menghambat laju proses aging dalam tubuhnya. [MKB. 2013;45(3):141–6] Aging Detection in Female Based on Antioxidant StatusAging is initiated by the accumulation of free radicals. Antioxidants which were known to control the reactivity of free radicals can be assumed as an anti aging. This study aimed to determine the age of the onset of aging in female based on antioxidant status. The analytical observational study with purposively random sampling design was conducted in Purwokerto in the year of 2008 with 34 females included toddlers, children, adolescents, adults, old, and elderly who were healthy, and live in Purwokerto. Antioxidant status was known by enzyme activities of superoxide dismutase (SOD), catalase, gluthathion peroxidase (GSH-PX) in the erythrocytes and malondialdehyde (MDA) levels in the plasma. Data were tested by analysis of varians (ANOVA). Antioxidant status in healthy female from toddlers to the elderly were prime as indicated by the high SOD, catalase, and GSH-PX activities that ranged 1,469+3.58–2,009+4.12 U/mg; 20.37+0.01–31.45+0.04 UI/mg; 79.03+0.01–225.2+0.04 μmol/g protein of erythrocytes, respectively; supported by low levels of MDA that ranged from 3,134+2.56–3,185+3.06 pmol/mL plasma. In general, the decrease in antioxidant status occurred in adults, so at that age female began to need additional antioxidant supplements in order to inhibit the rate of aging processes in the body. [MKB. 2013;45(3):141–6] DOI: http://dx.doi.org/10.15395/mkb.v45n3.143
Co-Authors - Isdiyarto . HERNAYANTI . HERY WINARSI Abdurohman Muslim Abdurrahman, Naufal Adhy Karyo Nugroho Adi Putra Setianto Adila Ashari Partono, Adila Ashari Adimas Wahyu Saputro Agung Budiarto, Agung Agustikasa, Dyah Kurniawati Akhmad Aminuddin Bama, Akhmad Aminuddin Alice Yuniati Alqodri, Achmad Fatih Andika Fajar Andreani Hanjani Anggun Nugroho, Anggun Anindita Putri Nurmalita Sari Anisa Agni Putri Aris Mukimin Arkhan Subari Artawan, I Nengah Arum Purwandari Bagus, Asep Bhagas Adhitya Ardia Pratama Bintoro Anang Subagyo, Bintoro Anang Budhy Kurniawan Budiwati Budiwati Budiyono Budiyono Chintya Fadila Laksmitaningrum Dadan Rosana Darmadi, Eko Agus Decy Lukitasari DEDDY MUCHTADI Dewitasari, Dinny Islamiah Dhanindra Prabowo, Dhanindra Dina Dina, Dina Dwi Harjanto, Ganang Dwi Ratmono Eddy Santoso Endang Kwartiningsih Endang Mastuti Eny Sri Widyastuti Erika Rani Eva Oktavia Ningrum, Eva Oktavia Febryanita, Renna Firdaus, Muhammad Sai FRANSISKA RUNGKAT ZAKARIA Gde Sastrawangsa, Gde Hanny Vistanty, Hanny Hatmoko, Bayu Dwi Hendri Widiyandari Heny Kusumayanti HERNAYANTI HERNAYANTI Heru Sukamto, Heru HERY WINARSI I Kadek Ariana, I Kadek Ida Hayu Dwimawanti Ihyaul Ulum Imam Ghozali Indah Nuraeni Intan Fatimah Hizbullah, Intan Fatimah Irfan Hafid Irnaning Handayani, Novarina Ischiadica Elharomy Isni Nurruhwati Jidanah Darmiyati Kuncoro Diharjo Kusumadewi, RR Karlina Aprilia Lila Yuwana Lilik Eka Radiati M. Refai Muslih Mety Nuraini Miratul Atiqah Mugihardjo, Herry Muhammad Baru Herman, Muhammad Baru Muhammad Setio Priambodo, Muhammad Mulyadi, Eka Octaviyatna Mulyoto ,, Mulyoto Nilawati Nilawati Nugraheni, Anggiyani Ratnaningtyas Eka Nur Cahyonowati Nur Kadarisman Nur Zen, Nur Nurkholis Nurkholis Nurtjahjo Dwi Sasongko Oktita Earning Hanifah Paryanto Paryanto Paryanto Prasetiyo, Agus Eko Purwaningrum, Fajar Adhi Rafika Rahmawati Rahma Ayu Maharani Rame Rame, Rame Rame, Rame Ratnawati Ratnawati Reny Yesiana, Reny Richardus Eko Indrajit Risha Aristiani N, Risha Rosita, Galuh Chynintya Rosita Ruliaty, Lisa Sari, Septi Shofwan Hanief, Shofwan Silvy Djayanti, Silvy Siwi P. M. Wijayanti Siwi P.M. Wijayanti SIWI PRAMATAMA MARS WIJAYANTI Stephani Novitasari Christianingsih Kaluti Suharno Suharno Suharto, Toni Suharto, Toni Sumarna Sumarna Sumarna, . Sumarno Sumarno Suratsih Suratsih Taufiq Bagus Prasojo Wicaksono, Kukuh Aryo Wisnu Ari Adi Yohanes Dwi Saputra, Yohanes Dwi Yuniar Mulyani Yuvita Avrie Diany